IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Association of Catsper1 or -2 with Ca(v)3.3 leads to suppression of T-type calcium channel activity. Association of Catsper1 or -2 with Ca(v)3.3 leads to suppression of T-type calcium channel activity. J. Biol. Chem. (0021-9258) 2006 imex curation Zhang D., Chen J., Saraf A., Cassar S., Han P., Rogers JC., Brioni JD., Sullivan JP., Gopalakrishnan M. di.zhang@abbott.com 09-April-2009: Contacted by JYOTI. davet Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang D., Chen J., Saraf A., Cassar S., Han P., Rogers JC., Brioni JD., Sullivan JP., Gopalakrishnan M. di.zhang@abbott.com Accepted 2009-MAR-17 by DAVET. 09-April-2009: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 davet Only protein-protein interactions imex curation Association of Catsper1 or -2 with Ca(v)3.3 leads to suppression of T-type calcium channel activity. Association of Catsper1 or -2 with Ca(v)3.3 leads to suppression of T-type calcium channel activity. J. Biol. Chem. (0021-9258) 2006 imex curation Zhang D., Chen J., Saraf A., Cassar S., Han P., Rogers JC., Brioni JD., Sullivan JP., Gopalakrishnan M. di.zhang@abbott.com 09-April-2009: Contacted by JYOTI. davet Only protein-protein interactions human-spermatocyte Homo sapiens spermatocyte spermatocyte Spermatocyte fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret immunostaining immunostaining Zhang D., Chen J., Saraf A., Cassar S., Han P., Rogers JC., Brioni JD., Sullivan JP., Gopalakrishnan M. di.zhang@abbott.com Accepted 2009-MAR-17 by DAVET. 09-April-2009: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 davet Only protein-protein interactions imex curation Association of Catsper1 or -2 with Ca(v)3.3 leads to suppression of T-type calcium channel activity. Association of Catsper1 or -2 with Ca(v)3.3 leads to suppression of T-type calcium channel activity. J. Biol. Chem. (0021-9258) 2006 imex curation Zhang D., Chen J., Saraf A., Cassar S., Han P., Rogers JC., Brioni JD., Sullivan JP., Gopalakrishnan M. di.zhang@abbott.com 09-April-2009: Contacted by JYOTI. davet Only protein-protein interactions in vitro In vitro pull down pull down mudpit multidimensional protein identification technology mudpit Zhang D., Chen J., Saraf A., Cassar S., Han P., Rogers JC., Brioni JD., Sullivan JP., Gopalakrishnan M. di.zhang@abbott.com Accepted 2009-MAR-17 by DAVET. 09-April-2009: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 davet Only protein-protein interactions imex curation Association of Catsper1 or -2 with Ca(v)3.3 leads to suppression of T-type calcium channel activity. Association of Catsper1 or -2 with Ca(v)3.3 leads to suppression of T-type calcium channel activity. J. Biol. Chem. (0021-9258) 2006 imex curation Zhang D., Chen J., Saraf A., Cassar S., Han P., Rogers JC., Brioni JD., Sullivan JP., Gopalakrishnan M. di.zhang@abbott.com 09-April-2009: Contacted by JYOTI. davet Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Zhang D., Chen J., Saraf A., Cassar S., Han P., Rogers JC., Brioni JD., Sullivan JP., Gopalakrishnan M. di.zhang@abbott.com Accepted 2009-MAR-17 by DAVET. 09-April-2009: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 davet Only protein-protein interactions imex curation Association of Catsper1 or -2 with Ca(v)3.3 leads to suppression of T-type calcium channel activity. Association of Catsper1 or -2 with Ca(v)3.3 leads to suppression of T-type calcium channel activity. J. Biol. Chem. (0021-9258) 2006 imex curation Zhang D., Chen J., Saraf A., Cassar S., Han P., Rogers JC., Brioni JD., Sullivan JP., Gopalakrishnan M. di.zhang@abbott.com 09-April-2009: Contacted by JYOTI. davet Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Zhang D., Chen J., Saraf A., Cassar S., Han P., Rogers JC., Brioni JD., Sullivan JP., Gopalakrishnan M. di.zhang@abbott.com Accepted 2009-MAR-17 by DAVET. 09-April-2009: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 davet Only protein-protein interactions imex curation cac1i_human Voltage-dependent T-type calcium channel subunit alpha-1I CACNA1I KIAA1120 Voltage-gated calcium channel subunit alpha Cav3.3 protein protein human Homo sapiens Human MAESASPPSSSAAAPAAEPGVTTEQPGPRSPPSSPPGLEEPLDGADPHVPHPDLAPIAFFCLRQTTSPRNWCIKMVCNPWFECVSMLVILLNCVTLGMYQPCDDMDCLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEENFTIQGDVALPPYYQPEEDDEMPFICSLSGDNGIMGCHEIPPLKEQGRECCLSKDDVYDFGAGRQDLNASGLCVNWNRYYNVCRTGSANPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMLEQRQRYLSSSTVASYAEPGDCYEEIFQYVCHILRKAKRRALGLYQALQSRRQALGPEAPAPAKPGPHAKEPRHYHGKTKGQGDEGRHLGSRHCQTLHGPASPGNDHSGRELCPQHSPLDATPHTLVQPIPATLASDPASCPCCQHEDGRRPSGLGSTDSGQEGSGSGSSAGGEDEADGDGARSSEDGASSELGKEEEEEEQADGAVWLCGDVWRETRAKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHEQPEELTNILEICNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIVGQADGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNIEEFDKLQEGLDSSGDPKLCPIPMTPNGHLDPSLPLGGHLGPAGAAGPAPRLSLQPDPMLVALGSRKSSVMSLGRMSYDQRSLSSSRSSYYGPWGRSAAWASRRSSWNSLKHKPPSAEHESLLSAERGGGARVCEVAADEGPPRAAPLHTPHAHHIHHGPHLAHRHRHHRRTLSLDNRDSVDLAELVPAVGAHPRAAWRAAGPAPGHEDCNGRMPSIAKDVFTKMGDRGDRGEDEEEIDYTLCFRVRKMIDVYKPDWCEVREDWSVYLFSPENRFRVLCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSLASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSDCMAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCHTRLLIHSMCTSHYLDIFITFIICLNVVTMSLEHYNQPTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSSLQFVSPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAELELEMAHGLGPGPRLPTGSPGAPGRGPGGAGGGGDTEGGLCRRCYSPAQENLWLDSVSLIIKDSLEGELTIIDNLSGSIFHHYSSPAGCKKCHHDKQEVQLAETEAFSLNSDRSSSILLGDDLSLEDPTACPPGRKDSKGELDPPEPMRVGDLGECFFPLSSTAVSPDPENFLCEMEEIPFNPVRSWLKHDSSQAPPSPFSPDASSPLLPMPAEFFHPAVSASQKGPEKGTGTGTLPKIALQGSWASLRSPRVNCTLLRQATGSDTSLDASPSSSAGSLQTTLEDSLTLSDSPRRALGPPAPAPGPRAGLSPAARRRLSLRGRGLFSLRGLRAHQRSHSSGGSTSPGCTHHDSMDPSDEEGRGGAGGGGAGSEHSETLSSLSLTSLFCPPPPPPAPGLTPARKFSSTSSLAAPGRPHAAALAHGLARSPSWAADRSKDPPGRAPLPMGLGPLAPPPQPLPGELEPGDAASKRKR 3CAE4D1C4289D08B ctsr1_human Cation channel sperm-associated protein 1 CATSPER1 protein protein human Homo sapiens Human MDQNSVPEKAQNEADTNNADRFFRSHSSPPHHRPGHSRALHHYELHHHGVPHQRGESHHPPEFQDFHDQALSSHVHQSHHHSEARNHGRAHGPTGFGLAPSQGAVPSHRSYGEDYHDELQRDGRRHHDGSQYGGFHQQSDSHYHRGSHHGRPQYLGENLSHYSSGVPHHGEASHHGGSYLPHGPNPYSESFHHSEASHLSGLQHDESQHHQVPHRGWPHHHQVHHHGRSRHHEAHQHGKSPHHGETISPHSSVGSYQRGISDYHSEYHQGDHHPSEYHHGDHPHHTQHHYHQTHRHRDYHQHQDHHGAYHSSYLHGDYVQSTSQLSIPHTSRSLIHDAPGPAASRTGVFPYHVAHPRGSAHSMTRSSSTIRSRVTQMSKKVHTQDISTKHSEDWGKEEGQFQKRKTGRLQRTRKKGHSTNLFQWLWEKLTFLIQGFREMIRNLTQSLAFETFIFFVVCLNTVMLVAQTFAEVEIRGEWYFMALDSIFFCIYVVEALLKIIALGLSYFFDFWNNLDFFIMAMAVLDFLLMQTHSFAIYHQSLFRILKVFKSLRALRAIRVLRRLSFLTSVQEVTGTLGQSLPSIAAILILMFTCLFLFSAVLRALFRKSDPKRFQNIFTTIFTLFTLLTLDDWSLIYMDSRAQGAWYIIPILVIYIIIQYFIFLNLVITVLVDSFQTALFKGLEKAKQERAARIQEKLLEDSLTELRAAEPKEVASEGTMLKRLIEKKFGTMTEKQQELLFHYLQLVASVEQEQQKFRSQAAVIDEIVDTTFEAGEEDFRN E09C4339F8AF5044 ctsr2_human Cation channel sperm-associated protein 2 CATSPER2 protein protein human Homo sapiens Human MAAYQQEEQMQLPRADAIRSRLIDTFSLIEHLQGLSQAVPRHTIRELLDPSRQKKLVLGDQHQLVRFSIKPQRIEQISHAQRLLSRLHVRCSQRPPLSLWAGWVLECPLFKNFIIFLVFLNTIILMVEIELLESTNTKLWPLKLTLEVAAWFILLIFILEILLKWLSNFSVFWKSAWNVFDFVVTMLSLLPEVVVLVGVTGQSVWLQLLRICRVLRSLKLLAQFRQIQIIILVLVRALKSMTFLLMLLLIFFYIFAVTGVYVFSEYTRSPRQDLEYHVFFSDLPNSLVTVFILFTLDHWYALLQDVWKVPEVSRIFSSIYFILWLLLGSIIFRSIIVAMMVTNFQNIRKELNEEMARREVQLKADMFKRQIIQRRKNMSHEALTSSHSKIEDSSRGASQQRESLDLSEVSEVESNYGATEEDLITSASKTEETLSKKREYQSSSCVSSTSSSYSSSSESRFSESIGRLDWETLVHENLPGLMEMDQDDRVWPRDSLFRYFELLEKLQYNLEERKKLQEFAVQALMNLEDK 8561CC0E9F3590B0 catsper1-cacna1i-1 3 6 unspecified role unspecified role prey prey human-seminal _fluid Human seminal fluid seminal _fluid Seminal fluid seminal plasma 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Identification of Cav3.3 as a CatSper1- and CatSper2-associated Protein from Human Sperm Extracts by GST Pulldown Approach Fig. 1 Accession number given for CACNA1l is for isoform 4 but it is not clear if this was realised at the time so interaction has been annotated to the parent UniProtKB entry. catsper2-cacna1i-1 3 8 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey human-seminal _fluid Human seminal fluid seminal _fluid Seminal fluid seminal plasma physical association physical association Identification of Cav3.3 as a CatSper1- and CatSper2-associated Protein from Human Sperm Extracts by GST Pulldown Approach Fig. 1 The bait proteins were a mixture of the N- and C-termini of CATSPER2. As it impossible to tell to which the prey bound, binding sites have not been annotated. Accession number given for CACNA1l is for isoform 4 but it is not clear if this was realised at the time so interaction has been annotated to the parent UniProtKB entry. catsper1-cacna1i-2 1 7 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association CatSper1 was detected in the proteins immunoprecipitated by anti-Cav3.3 antibody only when Cav3.3 and CatSper1 co-expressed in the cells. Fig. 2A Accession number given for CACNA1l is for isoform 4 but it is not clear if this was realised at the time so interaction has been annotated to the parent UniProtKB entry. catsper1-cacna1i-3 4 6 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association CatSper1 was detected in the proteins immunoprecipitated by anti-Cav3.3 antibody only when Cav3.3 and CatSper1 co-expressed in the cells. Fig. 2C Accession number given for CACNA1l is for isoform 4 but it is not clear if this was realised at the time so interaction has been annotated to the parent UniProtKB entry. catsper2-cacna1i-2 5 6 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association similar co-immunoprecipitation experiments demonstrating the association of CatSper2 with Cav3.3 in HEK cells. Fig. 2C Accession number given for CACNA1l is for isoform 4 but it is not clear if this was realised at the time so interaction has been annotated to the parent UniProtKB entry. catsper2-cacna1i-3 5 6 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association similar co-immunoprecipitation experiments demonstrating the association of CatSper2 with Cav3.3 in HEK cells. Fig. 2B Accession number given for CACNA1l is for isoform 4 but it is not clear if this was realised at the time so interaction has been annotated to the parent UniProtKB entry. catsper1-cacna1i-4 5 7 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association CatSper1 was detected in the proteins immunoprecipitated by anti-Cav3.3 antibody only when Cav3.3 and CatSper1 co-expressed in the cells. Fig. 2C Accession number given for CACNA1l is for isoform 4 but it is not clear if this was realised at the time so interaction has been annotated to the parent UniProtKB entry. catsper1-cacna1i-6 2 6 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction FRET Analysis of CatSper1 and Cav3.3 Interaction on Human Sperm Tail Fig. 7 anti-CACNA1I antibody was labelled with Alexa Fluor 488 dye as the donor (Cav3.3-488) and anti-CATSPER1 antibody with Alexa 555 dye as the acceptor Accession number given for CACNA1l is for isoform 4 but it is not clear if this was realised at the time so interaction has been annotated to the parent UniProtKB entry.