UniProt UniProt http://www.uniprot.org Role of a Cdc42p effector pathway in recruitment of the yeast septins to the presumptive bud site. Role of a Cdc42p effector pathway in recruitment of the yeast septins to the presumptive bud site. Mol. Biol. Cell (1059-1524) 2006 imex curation Iwase M., Luo J., Nagaraj S., Longtine M., Kim HB., Haarer BK., Caruso C., Tong Z., Pringle JR., Bi E. ebi@mail.med.upenn.edu 09-April-2009: Contacted by JYOTI. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Iwase M., Luo J., Nagaraj S., Longtine M., Kim HB., Haarer BK., Caruso C., Tong Z., Pringle JR., Bi E. ebi@mail.med.upenn.edu Accepted 2009-FEB-30 by JYOTI. 09-April-2009: Contacted by JYOTI. Mol. Biol. Cell (1059-1524) 2006 jyoti Only protein-protein interactions imex curation Role of a Cdc42p effector pathway in recruitment of the yeast septins to the presumptive bud site. Role of a Cdc42p effector pathway in recruitment of the yeast septins to the presumptive bud site. Mol. Biol. Cell (1059-1524) 2006 imex curation Iwase M., Luo J., Nagaraj S., Longtine M., Kim HB., Haarer BK., Caruso C., Tong Z., Pringle JR., Bi E. ebi@mail.med.upenn.edu 09-April-2009: Contacted by JYOTI. jyoti Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Iwase M., Luo J., Nagaraj S., Longtine M., Kim HB., Haarer BK., Caruso C., Tong Z., Pringle JR., Bi E. ebi@mail.med.upenn.edu Accepted 2009-FEB-30 by JYOTI. 09-April-2009: Contacted by JYOTI. Mol. Biol. Cell (1059-1524) 2006 jyoti Only protein-protein interactions imex curation Role of a Cdc42p effector pathway in recruitment of the yeast septins to the presumptive bud site. Role of a Cdc42p effector pathway in recruitment of the yeast septins to the presumptive bud site. Mol. Biol. Cell (1059-1524) 2006 imex curation Iwase M., Luo J., Nagaraj S., Longtine M., Kim HB., Haarer BK., Caruso C., Tong Z., Pringle JR., Bi E. ebi@mail.med.upenn.edu 09-April-2009: Contacted by JYOTI. jyoti Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Iwase M., Luo J., Nagaraj S., Longtine M., Kim HB., Haarer BK., Caruso C., Tong Z., Pringle JR., Bi E. ebi@mail.med.upenn.edu Accepted 2009-FEB-30 by JYOTI. 09-April-2009: Contacted by JYOTI. Mol. Biol. Cell (1059-1524) 2006 jyoti Only protein-protein interactions imex curation Role of a Cdc42p effector pathway in recruitment of the yeast septins to the presumptive bud site. Role of a Cdc42p effector pathway in recruitment of the yeast septins to the presumptive bud site. Mol. Biol. Cell (1059-1524) 2006 imex curation Iwase M., Luo J., Nagaraj S., Longtine M., Kim HB., Haarer BK., Caruso C., Tong Z., Pringle JR., Bi E. ebi@mail.med.upenn.edu 09-April-2009: Contacted by JYOTI. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Iwase M., Luo J., Nagaraj S., Longtine M., Kim HB., Haarer BK., Caruso C., Tong Z., Pringle JR., Bi E. ebi@mail.med.upenn.edu Accepted 2009-FEB-30 by JYOTI. 09-April-2009: Contacted by JYOTI. Mol. Biol. Cell (1059-1524) 2006 jyoti Only protein-protein interactions imex curation Role of a Cdc42p effector pathway in recruitment of the yeast septins to the presumptive bud site. Role of a Cdc42p effector pathway in recruitment of the yeast septins to the presumptive bud site. Mol. Biol. Cell (1059-1524) 2006 imex curation Iwase M., Luo J., Nagaraj S., Longtine M., Kim HB., Haarer BK., Caruso C., Tong Z., Pringle JR., Bi E. ebi@mail.med.upenn.edu 09-April-2009: Contacted by JYOTI. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast fluorescence fluorescence technology fluorescence immunostaining immunostaining Iwase M., Luo J., Nagaraj S., Longtine M., Kim HB., Haarer BK., Caruso C., Tong Z., Pringle JR., Bi E. ebi@mail.med.upenn.edu Accepted 2009-FEB-30 by JYOTI. 09-April-2009: Contacted by JYOTI. Mol. Biol. Cell (1059-1524) 2006 jyoti Only protein-protein interactions imex curation gic2_yeast GTPase-interacting component 2 D9740.18 YDR309C GIC2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTSASITNTGNETMNLPQMRSIWLDEDEEAEKLYGLQAQQFMGSDDEENLGITFINSDKPVLSNKKNIELPPLSPNSHPSCHHRRSNSNSAKSKESSSSSSSANKTNHKKVFLKLNLLKKKLLGAQPDIRGKGISTPFDFQHISHADTRNGFQDEQLQEPSSLSTEIKDDYTSSSSKRDSKSLNKAFVTERIPANRESKLISRSHENKTSRLSVARSISVTSSNYSKNTQGNNHSINGRVVSTSTMATSIFEYSPNASPKQFKNKSHALGHRYTNSTDSSESSLDFLKNYNFPTLLEDKPILDFLPRSQRSSAYRSLLETPNSNKDSAKAFFPSRQSPLPKRRNSIATPSPQSKFSYSDSPVNHRKSFDDVLYSFNQLEPLQT D1BE002BCD48EB03 D1BE002BCD48EB03 cdc11_yeast Cell division control protein 11 CDC11 PSL9 YJR076C J1833 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGIIDASSALRKRKHLKRGITFTVMIVGQSGSGRSTFINTLCGQQVVDTSTTILLPTDTSTEIDLQLREETVELEDDEGVKIQLNIIDTPGFGDSLDNSPSFEIISDYIRHQYDEILLEESRVRRNPRFKDGRVHCCLYLINPTGHGLKEIDVEFIRQLGSLVNIIPVISKSDSLTRDELKLNKKLIMEDIDRWNLPIYNFPFDEDEISDEDYETNMYLRTLLPFAIIGSNEVYEMGGDVGTIRGRKYPWGILDVEDSSISDFVILRNALLISHLHDLKNYTHEILYERYRTEALSGESVAAESIRPNLTKLNGSSSSSTTTRRNTNPFKQSNNINNDVLNPASDMHGQSTGENNETYMTREEQIRLEEERLKAFEERVQQELLLKRQELLQREKELREIEARLEKEAKIKQEE 5CFFD4CD1F6C57D4 5CFFD4CD1F6C57D4 cdc3_yeast Cell division control protein 3 CDC3 L8543.7 YLR314C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLKEEQVSIKQDPEQEERQHDQFNDVQIKQESQDHDGVDSQYTNGTQNDDSERFEAAESDVKVEPGLGMGITSSQSEKGQVLPDQPEIKFIRRQINGYVGFANLPKQWHRRSIKNGFSFNLLCVGPDGIGKTTLMKTLFNNDDIEANLVKDYEEELANDQEEEEGQGEGHENQSQEQRHKVKIKSYESVIEENGVKLNLNVIDTEGFGDFLNNDQKSWDPIIKEIDSRFDQYLDAENKINRHSINDKRIHACLYFIEPTGHYLKPLDLKFMQSVYEKCNLIPVIAKSDILTDEEILSFKKTIMNQLIQSNIELFKPPIYSNDDAENSHLSERLFSSLPYAVIGSNDIVENYSGNQVRGRSYPWGVIEVDNDNHSDFNLLKNLLIKQFMEELKERTSKILYENYRSSKLAKLGIKQDNSVFKEFDPISKQQEEKTLHEAKLAKLEIEMKTVFQQKVSEKEKKLQKSETELFARHKEMKEKLTKQLKALEDKKKQLELSINSASPNVNHSPVPTKKKGFLR DCCD4C8C16AA5E25 DCCD4C8C16AA5E25 gic1_yeast GTPase-interacting component 1 YHR061C GIC1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTEGKRLQQMELPQMKSIWIDEDQEMEKLYGFQVRQRFMNGPSTDSDEDADEDLGIVLVDSKKLALPNKNNIKLPPLPNYMTINPNINSNHKSLTNKKKNFLGMFKKKDLLSRRHGSAKTAKQSSISTPFDFHHISHANGKREDNPLESHEEKHDVESLVKFTSLAPQPRPDSNVSSKYSNVVMNDSSRIVSSSTIATTMDSHHDGNETNNTPNGNKQLDSPTDLEMTLEDLRNYTFPSVLGDSVSEKTNPSSPSVSSFSGKFKPRELSALHTPELGNCFNVDQSLNSPGNRISVDDVLKFYYQCSETSTPRNT C6B2FE253799500B cdc12_yeast Cell division control protein 12 CDC12 CLA10 PSL7 YHR107C Septin protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAATATAAPVPPPVGISNLPNQRYKIVNEEGGTFTVMLCGESGLGKTTFINTLFQTVLKRADGQQHRQEPIRKTVEIDITRALLEEKHFELRVNVIDTPGFGDNVNNNKAWQPLVDFIDDQHDSYMRQEQQPYRTKKFDLRVHAVLYFIRPTGHGLKPIDIETMKRLSTRANLIPVIAKADTLTAQELQQFKSRIRQVIEAQEIRIFTPPLDADSKEDAKSGSNPDSAAVEHARQLIEAMPFAIVGSEKKFDNGQGTQVVARKYPWGLVEIENDSHCDFRKLRALLLRTYLLDLISTTQEMHYETYRRLRLEGHENTGEGNEDFTLPAIAPARKLSHNPRYKEEENALKKYFTDQVKAEEQRFRQWEQNIVNERIRLNGDLEEIQGKVKKLEEQVKSLQVKKSHLK A72DC393A6DBF924 A72DC393A6DBF924 gic1-cdc11-1 1 9 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Fig 8A gic1-cdc12-2 1 9 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Fig 8A gic2-cdc12-2 1 10 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Fig 8A gic2-cdc11-1 1 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Fig 8A gic1-cdc12-3 2 9 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) 10 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Fig 8B gic2-cdc12-3 2 6 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) 10 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Fig 8B gic1-cdc3-1 2 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 9 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) physical association physical association Fig 8B gic2-cdc3-1 2 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 6 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) physical association physical association Fig 8B gic1-cdc12-4 3 10 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 9 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) physical association physical association Fig 8B gic2-cdc12-4 3 6 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) 10 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Fig 8B gic1-cdc3-2 3 9 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Fig 8B gic2-cdc3-2 3 6 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Fig 8B cdc11-cdc3-1 4 9 unspecified role unspecified role prey prey c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Fig 8C, 8D cdc11-gic2-1 4 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 8D gic2-cdc3-3 5 8 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig S1