IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits. Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits. J. Biol. Chem. (0021-9258) 2004 imex curation Galani K., Nissan TA., Petfalski E., Tollervey D., Hurt E. cg5@ix.urz.uniheidelberg.de davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast tap tandem affinity purification TAP TAP tap fingerprinting peptide massfingerprinting fingerprinting Galani K., Nissan TA., Petfalski E., Tollervey D., Hurt E. cg5@ix.urz.uniheidelberg.de Accepted 2009-SEP-24 by DAVET. J. Biol. Chem. (0021-9258) 2004 davet Only protein-protein interactions imex curation rix1_yeast Pre-rRNA-processing protein RIX1 Ribosomal export protein 1 Involved in processing IST2 protein 2 RIX1 IPI2 YHR197W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEEFIAVSTLARNLEIAKGNEFHTILATLRSPVYINEQLLKSELSFLVTKILKLIRSGNDFDLWKGCHTSVVTCAYNPLVLSTHGGQLLAAIYSRLEQKTGFYSSVISSSHGKQLFNTLISSVAIIIDLMKNKPTLSREALVPKLKAIIPTLITLSQYEPELVLPVLQRILKRNTTTFKPFTNKFRTVLINLIISDYASLGTKTQRLVCENFAYLHLLKIQVSDTSDDETQAHHKIYADSNWRTGLMSILSQFKPIIQLCGEILDFEQDNELYKLIKSLPVIDESNNKEEFLPSLKLDFNAPLTLWEIPQRLSLLADMLVAFISLPTPFPIRVPLGGINSLCEVLLGVSNKYLPLKKELRHDNELNGVINTILPQIQFQGIRLWEIMVSKYGKCGLSFFEGILSSIELFIPLKKKSNNEIDFNVVGSLKFEFATVFRLVNMILSHLGHQLNIISVISQLIEVALFLSHDKTLIDSLFKNRKSIMKQQTKTKQSKRSKSAEGAFSDIYTHPELFVCKNSMNWFNEINDFFITALNNWILPSTPHIQILKYSITQSLRLKERFGYIPESFVNLLRCEVLHPGSERVSILPIAISLLKNINDDMFELLCHPKVPVGMVYQLHKPLDLGEDGEVRDDINKKEVETNESSSNANTGLETLKALENLENVTIPEPKHEVPKVVDDTAIFKKRSVEEVIERESTSSHKKVKFVEETTVDNGEELIVKKAVSQTKEEEKPMEDSEDEEQEEFEIPAIELSDDEEEEEEEE 14A0CE9A21F11711 14A0CE9A21F11711 mdn1_yeast Midasin MDN1 L8004.13 MIDAS-containing protein Dynein-related AAA-ATPase REA1 REA1 Ribosome export/assembly protein 1 YLR106C L2901 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQDRILLDLDVVNQRLILFNSAFPSDAIEAPFHFSNKESTSENLDNLAGTILHSRSITGHVFLYKHIFLEIVARWIKDSKKKDYVLVIEKLASIITIFPVAMPLIEDYLDKENDHFITILQNPSTQKDSDMFKILLAYYRLLYHNKEVFARFIQPDILYQLVDLLTKEQENQVVIFLALKVLSLYLDMGEKTLNDMLDTYIKSRDSLLGHFEGDSGIDYSFLELNEAKRCANFSKLPSVPECFTIEKKSSYFIIEPQDLSTKVASICGVIVPKVHTIHDKVFYPLTFVPTHKTVSSLRQLGRKIQNSTPIMLIGKAGSGKTFLINELSKYMGCHDSIVKIHLGEQTDAKLLIGTYTSGDKPGTFEWRAGVLATAVKEGRWVLIEDIDKAPTDVLSILLSLLEKRELTIPSRGETVKAANGFQLISTVRINEDHQKDSSNKIYNLNMIGMRIWNVIELEEPSEEDLTHILAQKFPILTNLIPKLIDSYKNVKSIYMNTKFISLNKGAHTRVVSVRDLIKLCERLDILFKNNGINKPDQLIQSSVYDSIFSEAADCFAGAIGEFKALEPIIQAIGESLDIASSRISLFLTQHVPTLENLDDSIKIGRAVLLKEKLNIQKKSMNSTLFAFTNHSLRLMEQISVCIQMTEPVLLVGETGTGKTTVVQQLAKMLAKKLTVINVSQQTETGDLLGGYKPVNSKTVAVPIQENFETLFNATFSLKKNEKFHKMLHRCFNKNQWKNVVKLWNEAYKMAQSILKITNTENENENAKKKKRRLNTHEKKLLLDKWADFNDSVKKFEAQSSSIENSFVFNFVEGSLVKTIRAGEWLLLDEVNLATADTLESISDLLTEPDSRSILLSEKGDAEPIKAHPDFRIFACMNPATDVGKRDLPMGIRSRFTEIYVHSPERDITDLLSIIDKYIGKYSVSDEWVGNDIAELYLEAKKLSDNNTIVDGSNQKPHFSIRTLTRTLLYVTDIIHIYGLRRSLYDGFCMSFLTLLDQKSEAILKPVIEKFTLGRLKNVKSIMSQTPPSPGPDYVQFKHYWMKKGPNTIQEQAHYIITPFVEKNMMNLVRATSGKRFPVLIQGPTSSGKTSMIKYLADITGHKFVRINNHEHTDLQEYLGTYVTDDTGKLSFKEGVLVEALRKGYWIVLDELNLAPTDVLEALNRLLDDNRELFIPETQEVVHPHPDFLLFATQNPPGIYGGRKILSRAFRNRFLELHFDDIPQDELEIILRERCQIAPSYAKKIVEVYRQLSIERSASRLFEQKNSFATLRDLFRWALRDAVGYEQLAASGYMLLAERCRTPQEKVTVKKTLEKVMKVKLDMDQYYASLEDKSLEAIGSVTWTKGMRRLSVLVSSCLKNKEPVLLVGETGCGKTTICQLLAQFMGRELITLNAHQNTETGDILGAQRPVRNRSEIQYKLIKSLKTALNIANDQDVDLKELLQLYSKSDNKNIAEDVQLEIQKLRDSLNVLFEWSDGPLIQAMRTGNFFLLDEISLADDSVLERLNSVLEPERSLLLAEQGSSDSLVTASENFQFFATMNPGGDYGKKELSPALRNRFTEIWVPSMEDFNDVNMIVSSRLLEDLKDLANPIVKFSEWFGKKLGGGNATSGVISLRDILAWVEFINKVFPKIQNKSTALIQGASMVFIDALGTNNTAYLAENENDLKSLRTECIIQLLKLCGDDLELQQIETNEIIVTQDELQVGMFKIPRFPDAQSSSFNLTAPTTASNLVRVVRAMQVHKPILLEGSPGVGKTSLITALANITGNKLTRINLSEQTDLVDLFGADAPGERSGEFLWHDAPFLRAMKKGEWVLLDEMNLASQSVLEGLNACLDHRGEAYIPELDISFSCHPNFLVFAAQNPQYQGGGRKGLPKSFVNRFSVVFIDMLTSDDLLLIAKHLYPSIEPDIIAKMIKLMSTLEDQVCKRKLWGNSGSPWEFNLRDTLRWLKLLNQYSICEDVDVFDFVDIIVKQRFRTISDKNKAQLLIEDIFGKFSTKENFFKLTEDYVQINNEVALRNPHYRYPITQNLFPLECNVAVYESVLKAINNNWPLVLVGPSNSGKTETIRFLASILGPRVDVFSMNSDIDSMDILGGYEQVDLTRQISYITEELTNIVREIISMNMKLSPNATAIMEGLNLLKYLLNNIVTPEKFQDFRNRFNRFFSHLEGHPLLKTMSMNIEKMTEIITKEASVKFEWFDGMLVKAVEKGHWLILDNANLCSPSVLDRLNSLLEIDGSLLINECSQEDGQPRVLKPHPNFRLFLTMDPKYGELSRAMRNRGVEIYIDELHSRSTAFDRLTLGFELGENIDFVSIDDGIKKIKLNEPDMSIPLKHYVPSYLSRPCIFAQVHDILLLSDEEPIEESLAAVIPISHLGEVGKWANNVLNCTEYSEKKIAERLYVFITFLTDMGVLEKINNLYKPANLKFQKALGLHDKQLTEETVSLTLNEYVLPTVSKYSDKIKSPESLYLLSSLRLLLNSLNALKLINEKSTHGKIDELTYIELSAAAFNGRHLKNIPRIPIFCILYNILTVMSENLKTESLFCGSNQYQYYWDLLVIVIAALETAVTKDEARLRVYKELIDSWIASVKSKSDIEITPFLNINLEFTDVLQLSRGHSITLLWDIFRKNYPTTSNSWLAFEKLINLSEKFDKVRLLQFSESYNSIKDLMDVFRLLNDDVLNNKLSEFNLLLSKLEDGINELELISNKFLNKRKHYFADEFDNLIRYTFSVDTAELIKELAPASSLATQKLTKLITNKYNYPPIFDVLWTEKNAKLTSFTSTIFSSQFLEDVVRKSNNLKSFSGNQIKQSISDAELLLSSTIKCSPNLLKSQMEYYKNMLLSWLRKVIDIHVGGDCLKLTLKELCSLIEEKTASETRVTFAEYIFPALDLAESSKSLEELGEAWITFGTGLLLLFVPDSPYDPAIHDYVLYDLFLKTKTFSQNLMKSWRNVRKVISGDEEIFTEKLINTISDDDAPQSPRVYRTGMSIDSLFDEWMAFLSSTMSSRQIKELVSSYKCNSDQSDRRLEMLQQNSAHFLNRLESGYSKFADLNDILAGYIYSINFGFDLLKLQKSKDRASFQISPLWSMDPINISCAENVLSAYHELSRFFKKGDMEDTSIEKVLMYFLTLFKFHKRDTNLLEIFEAALYTLYSRWSVRRFRQEQEENEKSNMFKFNDNSDDYEADFRKLFPDYEDTALVTNEKDISSPENLDDIYFKLADTYISVFDKDHDANFSSELKSGAIITTILSEDLKNTRIEELKSGSLSAVINTLDAETQSFKNTEVFGNIDFYHDFSIPEFQKAGDIIETVLKSVLKLLKQWPEHATLKELYRVSQEFLNYPIKTPLARQLQKIEQIYTYLAEWEKYASSEVSLNNTVKLITDLIVSWRKLELRTWKGLFNSEDAKTRKSIGKWWFYLYESIVISNFVSEKKETAPNATLLVSSLNLFFSKSTLGEFNARLDLVKAFYKHIQLIGLRSSKIAGLLHNTIKFYYQFKPLIDERITNGKKSLEKEIDDIILLASWKDVNVDALKQSSRKSHNNLYKIVRKYRDLLNGDAKTIIEAGLLYSNENKLKLPTLKQHFYEDPNLEASKNLVKEISTWSMRAAPLRNIDTVASNMDSYLEKISSQEFPNFADLASDFYAEAERLRKETPNVYTKENKKRLAYLKTQKSKLLGDALKELRRIGLKVNFREDIQKVQSSTTTILANIAPFNNEYLNSSDAFFFKILDLLPKLRSAASNPSDDIPVAAIERGMALAQSLMFSLITVRHPLSEFTNDYCKINGMMLDLEHFTCLKGDIVHSSLKANVDNVRLFEKWLPSLLDYAAQTLSVISKYSATSEQQKILLDAKSTLSSFFVHFNSSRIFDSSFIESYSRFELFINELLKKLENAKETGNAFVFDIIIEWIKANKGGPIKKEQKRGPSVEDVEQAFRRTFTSIILSFQKVIGDGIESISETDDNWLSASFKKVMVNVKLLRSSVVSKNIETALSLLKDFDFTTTESIYVKSVISFTLPVITRYYNAMTVVLERSRIYYTNTSRGMYILSTILHSLAKNGFCSPQPPSEEVDDKNLQEGTGLGDGEGAQNNNKDVEQDEDLTEDAQNENKEQQDKDERDDENEDDAVEMEGDMAGELEDLSNGEENDDEDTDSEEEELDEEIDDLNEDDPNAIDDKMWDDKASDNSKEKDTDQNLDGKNQEEDVQAAENDEQQRDNKEGGDEDPNAPEDGDEEIENDENAEEENDVGEQEDEVKDEEGEDLEANVPEIETLDLPEDMNLDSEHEESDEDVDMSDGMPDDLNKEEVGNEDEEVKQESGIESDNENDEPGPEEDAGETETALDEEEGAEEDVDMTNDEGKEDEENGPEEQAMSDEEELKQDAAMEENKEKGGEQNTEGLDGVEEKADTEDIDQEAAVQQDSGSKGAGADATDTQEQDDVGGSGTTQNTYEEDQEDVTKNNEESREEATAALKQLGDSMKEYHRRRQDIKEAQTNGEEDENLEKNNERPDEFEHVEGANTETDTQALGSATQDQLQTIDEDMAIDDDREEQEVDQKELVEDADDEKMDIDEEEMLSDIDAHDANNDVDSKKSGFIGKRKSEEDFENELSNEHFSADQEDDSEIQSLIENIEDNPPDASASLTPERSLEESRELWHKSEISTADLVSRLGEQLRLILEPTLATKLKGDYKTGKRLNMKRIIPYIASQFRKDKIWLRRTKPSKRQYQIMIALDDSKSMSESKCVKLAFDSLCLVSKTLTQLEAGGLSIVKFGENIKEVHSFDQQFSNESGARAFQWFGFQETKTDVKKLVAESTKIFERARAMVHNDQWQLEIVISDGICEDHETIQKLVRRARENKIMLVFVIIDGITSNESILDMSQVNYIPDQYGNPQLKITKYLDTFPFEFYVVVHDISELPEMLSLILRQYFTDLASS E4E873BEDF6E1E5B E4E873BEDF6E1E5B ipi3_yeast Pre-rRNA-processing protein IPI3 Involved in processing IST2 protein 3 YNL182C N1636 IPI3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDEQVIFTTNTSGTIASVHSFEQINLRQCSTQSRNSCVQVGNKYLFIAQAQKALINVYNLSGSFKRESVEQRLPLPEILKCLEVVENDGVQYDRIQGVNHNLPDFNLPYLLLGSTESGKLYIWELNSGILLNVKPMAHYQSITKIKSILNGKYIITSGNDSRVIIWQTVDLVSASNDDPKPLCILHDHTLPVTDFQVSSSQGKFLSCTDTKLFTVSQDATIRCYDLSLIGSKKKQKANENDVSIGKTPVLLATFTTPYSIKSIVLDPADRACYIGTAEGCFSLNLFYKLKGNAIVNLLQSAGVNTVQKGRVFSLVQRNSLTGGENEDLDALYAMGQLVCENVLNSNVSCLEISMDGTLLLIGDTEGKVSIAEIYSKQIIRTIQTLTTSQDSVGEVTNLLTNPYRLERGNLLFEGESKGKQPSNNNGHNFMKIPNLQRVIFDGKNKGHLHDIWYQIGEPEAETDPNLALPLNDFNAYLEQVKTQESIFSHIGKVSSNVKVIDNKIDATSSLDSNAAKDEEITELKTNIEALTHAYKELRDMHEKLYEEHQQMLDKQ 8EED6854DF9405A6 8EED6854DF9405A6 ipi1_yeast Pre-rRNA-processing protein IPI1 YHR085W IPI1 Involved in processing IST2 protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTKSRKQKQKKQDFLRKKLKVGKPKEKARNATDTSFVSKTISIRNQHLDQNPHDLTKRLTLLKHHNINVRKETLTTFQKSIPSIIKSRLMTPLLTQSIPLICDESQQVRQGLIDLVDEIGSHDAEILKLHCNIFVLYINMAMTHIVTQIQADSTKFLSHLLKYCGDEVVRKSWVKLLNGVFGVLGWGQVGKNDSASIVQTKKRNAKYVTIHLNALYTLVEYGCQDERARSDGDTAETTEDSGTLRNPYLIPDYPQPFEHLKLFTRELKVQDATSSGVNATLLSLATQDIDTRKAVFIEQFLPIVRKKIEVIIKEGGECGKSANKLKTLLAKIFD 1AB0B318BBFC8096 1AB0B318BBFC8096 rix1-ipi3-1 1 2 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey association association The observation that Rix1, Ipi1, Ipi3, and Rea1 co-enrich during biochemical purification indicates that these four proteins could be organized in a complex attached to nascent 60 S subunits. Fig. 1A rix1-ipi3-2 1 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 2 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association However, Rix1, Ipi1, and Ipi3 remained bound under these conditions and were also resistant to 200 mM MgCl2, but they eluted by SDS-sample buffer (Fig. 1B, lane 3) Fig. 1B Complex is resistant to high salt or magnesium.