IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Metal-protein complex-mediated transport and delivery of Ni2+ to TCR/MHC contact sites in nickel-specific human T cell activation. Metal-protein complex-mediated transport and delivery of Ni2+ to TCR/MHC contact sites in nickel-specific human T cell activation. J. Immunol. (0022-1767) 2004 imex curation Thierse HJ., Moulon C., Allespach Y., Zimmermann B., Doetze A., Kuppig S., Wild D., Herberg F., Weltzien HU. SPR signal measured in relative units was taken as a measure of author confidence in the interaction thierse@immunbio.mpg.de 14-May-2009: Contacted by JYOTI. 2009-03-30: Hermann-Josef Thierse, Dept. of Dermatoloy, Univ. Heidelberg, Germany. davet Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Thierse HJ., Moulon C., Allespach Y., Zimmermann B., Doetze A., Kuppig S., Wild D., Herberg F., Weltzien HU. SPR signal measured in relative units was taken as a measure of author confidence in the interaction thierse@immunbio.mpg.de thierse@immunbio.mpg.de Accepted 2009-MAY-7 by DAVET. 2009-03-30: Hermann-Josef Thierse, Dept. of Dermatoloy, Univ. Heidelberg, Germany. J. Immunol. (0022-1767) 2004 davet Only protein-protein interactions imex curation albu_pig Albumin ALB protein protein pig Sus scrofa (Pig) Pig MKWVTFISLLFLFSSAYSRGVFRRDTYKSEIAHRFKDLGEQYFKGLVLIAFSQHLQQCPYEEHVKLVREVTEFAKTCVADESAENCDKSIHTLFGDKLCAIPSLREHYGDLADCCEKEEPERNECFLQHKNDNPDIPKLKPDPVALCADFQEDEQKFWGKYLYEIARRHPYFYAPELLYYAIIYKDVFSECCQAADKAACLLPKIEHLREKVLTSAAKQRLKCASIQKFGERAFKAWSLARLSQRFPKADFTEISKIVTDLAKVHKECCHGDLLECADDRADLAKYICENQDTISTKLKECCDKPLLEKSHCIAEAKRDELPADLNPLEHDFVEDKEVCKNYKEAKHVFLGTFLYEYSRRHPDYSVSLLLRIAKIYEATLEDCCAKEDPPACYATVFDKFQPLVDEPKNLIKQNCELFEKLGEYGFQNALIVRYTKKVPQVSTPTLVEVARKLGLVGSRCCKRPEEERLSCAEDYLSLVLNRLCVLHEKTPVSEKVTKCCTESLVNRRPCFSALTPDETYKPKEFVEGTFTFHADLCTLPEDEKQIKKQTALVELLKHKPHATEEQLRTVLGNFAAFVQKCCAAPDHEACFAVEGPKFVIEIRGILA 7B8DA13543CA99D8 albu_human Albumin GIG42 ALB PRO0903 PRO1708 PRO2044 PRO2619 PRO2675 UNQ696/PRO1341 GIG20 protein protein human Homo sapiens Human MKWVTFISLLFLFSSAYSRGVFRRDAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL DIP protein P02768 original sequence version: 132 DIP protein P02768-1 original sequence version: 174 F88FF61DD242E818 albu_bovin Albumin ALB BSA protein protein bovin Bos taurus (Bovine) Bovine MKWVTFISLLLLFSSAYSRGVFRRDTHKSEIAHRFKDLGEEHFKGLVLIAFSQYLQQCPFDEHVKLVNELTEFAKTCVADESHAGCEKSLHTLFGDELCKVASLRETYGDMADCCEKQEPERNECFLSHKDDSPDLPKLKPDPNTLCDEFKADEKKFWGKYLYEIARRHPYFYAPELLYYANKYNGVFQECCQAEDKGACLLPKIETMREKVLASSARQRLRCASIQKFGERALKAWSVARLSQKFPKAEFVEVTKLVTDLTKVHKECCHGDLLECADDRADLAKYICDNQDTISSKLKECCDKPLLEKSHCIAEVEKDAIPENLPPLTADFAEDKDVCKNYQEAKDAFLGSFLYEYSRRHPEYAVSVLLRLAKEYEATLEECCAKDDPHACYSTVFDKLKHLVDEPQNLIKQNCDQFEKLGEYGFQNALIVRYTRKVPQVSTPTLVEVSRSLGKVGTRCCTKPESERMPCTEDYLSLILNRLCVLHEKTPVSEKVTKCCTESLVNRRPCFSALTPDETYVPKAFDEKLFTFHADICTLPDTEKQIKKQTALVELLKHKPKATEEQLKTVMENFVAFVDKCCAADDKEACFAVEGPKLVVSTQTALA 39167DFE768585D4 albu_mouse Albumin Alb Alb1 Alb-1 protein protein mouse Mus musculus Mouse MKWVTFLLLLFVSGSAFSRGVFRREAHKSEIAHRYNDLGEQHFKGLVLIAFSQYLQKCSYDEHAKLVQEVTDFAKTCVADESAANCDKSLHTLFGDKLCAIPNLRENYGELADCCTKQEPERNECFLQHKDDNPSLPPFERPEAEAMCTSFKENPTTFMGHYLHEVARRHPYFYAPELLYYAEQYNEILTQCCAEADKESCLTPKLDGVKEKALVSSVRQRMKCSSMQKFGERAFKAWAVARLSQTFPNADFAEITKLATDLTKVNKECCHGDLLECADDRAELAKYMCENQATISSKLQTCCDKPLLKKAHCLSEVEHDTMPADLPAIAADFVEDQEVCKNYAEAKDVFLGTFLYEYSRRHPDYSVSLLLRLAKKYEATLEKCCAEANPPACYGTVLAEFQPLVEEPKNLVKTNCDLYEKLGEYGFQNAILVRYTQKAPQVSTPTLVEAARNLGRVGTKCCTLPEDQRLPCVEDYLSAILNRVCLLHEKTPVSEHVTKCCSGSLVERRPCFSALTVDETYVPKEFKAETFTFHSDICTLPEKEKQIKKQTALAELVKHKPKATAEQLKTVMDDFAQFLDTCCKAADKDTCFSTEGPNLVTRCKDALA 292F7C7EED3A61B4 nickel(2+) Nickel(2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) his-his Histidine dipepetide protein protein in vitro In vitro HH An amino acid sequence which is not represented in UniProt 61E1800000000000 albu_chick Albumin ALB Alpha-livetin protein protein chick Gallus gallus (Chicken) Chicken MKWVTLISFIFLFSSATSRNLQRFARDAEHKSEIAHRYNDLKEETFKAVAMITFAQYLQRCSYEGLSKLVKDVVDLAQKCVANEDAPECSKPLPSIILDEICQVEKLRDSYGAMADCCSKADPERNECFLSFKVSQPDFVQPYQRPASDVICQEYQDNRVSFLGHFIYSVARRHPFLYAPAILSFAVDFEHALQSCCKESDVGACLDTKEIVMREKAKGVSVKQQYFCGILKQFGDRVFQARQLIYLSQKYPKAPFSEVSKFVHDSIGVHKECCEGDMVECMDDMARMMSNLCSQQDVFSGKIKDCCEKPIVERSQCIMEAEFDEKPADLPSLVEKYIEDKEVCKSFEAGHDAFMAEFVYEYSRRHPEFSIQLIMRIAKGYESLLEKCCKTDNPAECYANAQEQLNQHIKETQDVVKTNCDLLHDHGEADFLKSILIRYTKKMPQVPTDLLLETGKKMTTIGTKCCQLGEDRRMACSEGYLSIVIHDTCRKQETTPINDNVSQCCSQLYANRRPCFTAMGVDTKYVPPPFNPDMFSFDEKLCSAPAEEREVGQMKLLINLIKRKPQMTEEQIKTIADGFTAMVDKCCKQSDINTCFGEEGANLIVQSRATLGIGA E59E4BBCAEC066C6 ni2plus-albu-1 1 3 unspecified role unspecified role prey prey human-serum Homo sapiens serum Serum 6 unspecified role unspecified role bait bait direct interaction direct interaction author score author-based confidence author score 1425 RU The results of the Biacore analysis (Fig. 3A) also correlate well with the albumins biological activities (Table II) in that the strongest binding was observed for HSA, reduced but significant binding was observed for BSA, CSA, PSA, and MSA, and almost no interaction was observed for DSA. Figs. 3A, 3B ni2plus-albu-2 1 4 unspecified role unspecified role prey prey bovin-serum Bovine serum serum Serum 6 unspecified role unspecified role bait bait direct interaction direct interaction author score author-based confidence author score 326 RU The results of the Biacore analysis (Fig. 3A) also correlate well with the albumins biological activities (Table II) in that the strongest binding was observed for HSA, reduced but significant binding was observed for BSA, CSA, PSA, and MSA, and almost no interaction was observed for DSA. Fig. 3A ni2plus-albu-3 1 6 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey chick-serum Chick serum serum Serum direct interaction direct interaction author score author-based confidence author score 167 RU The results of the Biacore analysis (Fig. 3A) also correlate well with the albumins biological activities (Table II) in that the strongest binding was observed for HSA, reduced but significant binding was observed for BSA, CSA, PSA, and MSA, and almost no interaction was observed for DSA. Fig. 3A ni2plus-albu-4 1 6 unspecified role unspecified role bait bait 2 unspecified role unspecified role prey prey pig-serum Pig serum serum Serum direct interaction direct interaction author score author-based confidence author score 220 RU The results of the Biacore analysis (Fig. 3A) also correlate well with the albumins biological activities (Table II) in that the strongest binding was observed for HSA, reduced but significant binding was observed for BSA, CSA, PSA, and MSA, and almost no interaction was observed for DSA. Fig. 3A ni2plus-albu-5 1 5 unspecified role unspecified role prey prey mouse-serum Mouse serum serum Serum 6 unspecified role unspecified role bait bait direct interaction direct interaction author score author-based confidence author score 205 RU The results of the Biacore analysis (Fig. 3A) also correlate well with the albumins biological activities (Table II) in that the strongest binding was observed for HSA, reduced but significant binding was observed for BSA, CSA, PSA, and MSA, and almost no interaction was observed for DSA. Fig. 3A ni2plus-albu-6 1 6 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction Consequently, the Biacore-determined binding constant of 7.5 10 4 M (Fig. 3C) is lower than most previously reported affinities for albumin-Ni interactions Figs. 3C Temp.=20C, pH=7.4 ni2plus-albu-7 1 3 unspecified role unspecified role prey prey human-serum Homo sapiens serum Serum 3 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro 6 unspecified role unspecified role bait bait physical association physical association We also found that coincubation of HSA-Ni with either a dihistidyl peptide or the N-terminal tetrapeptide of HSA and subsequent dialysis, already at 1:1 molar ratios, resulted in the significant loss of HSAassociated Ni (Fig. 3D). Figs. 3D The synthetic HSA tetrapeptide competed with full length HSA for nickel binding. ni2plus-albu-8 1 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey in vitro In vitro 3 unspecified role unspecified role prey prey in vitro In vitro physical association physical association We also found that coincubation of HSA-Ni with either a dihistidyl peptide or the N-terminal tetrapeptide of HSA and subsequent dialysis, already at 1:1 molar ratios, resulted in the significant loss of HSAassociated Ni (Fig. 3D). Figs. 3D The synthetic HSA tetrapeptide competed with full length HSA for nickel binding.