IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Bms1p, a G-domain-containing protein, associates with Rcl1p and is required for 18S rRNA biogenesis in yeast. Bms1p, a G-domain-containing protein, associates with Rcl1p and is required for 18S rRNA biogenesis in yeast. RNA (1355-8382) 2001 imex curation Wegierski T., Billy E., Nasr F., Filipowicz W. Filipowi@fmi.ch 27-Oct-2007: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wegierski T., Billy E., Nasr F., Filipowicz W. Filipowi@fmi.ch Accepted 2009-APR-18 by ORCHARD. RNA (1355-8382) 2001 Only protein-protein interactions imex curation Bms1p, a G-domain-containing protein, associates with Rcl1p and is required for 18S rRNA biogenesis in yeast. Bms1p, a G-domain-containing protein, associates with Rcl1p and is required for 18S rRNA biogenesis in yeast. RNA (1355-8382) 2001 imex curation Wegierski T., Billy E., Nasr F., Filipowicz W. Filipowi@fmi.ch 27-Oct-2007: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Wegierski T., Billy E., Nasr F., Filipowicz W. Filipowi@fmi.ch Accepted 2009-APR-18 by ORCHARD. 27-Oct-2007: Contacted by JYOTI. RNA (1355-8382) 2001 Only protein-protein interactions imex curation Bms1p, a G-domain-containing protein, associates with Rcl1p and is required for 18S rRNA biogenesis in yeast. Bms1p, a G-domain-containing protein, associates with Rcl1p and is required for 18S rRNA biogenesis in yeast. RNA (1355-8382) 2001 imex curation Wegierski T., Billy E., Nasr F., Filipowicz W. Filipowi@fmi.ch 27-Oct-2007: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Wegierski T., Billy E., Nasr F., Filipowicz W. 10-30% glycerol Filipowi@fmi.ch Accepted 2009-APR-18 by ORCHARD. 27-Oct-2007: Contacted by JYOTI. RNA (1355-8382) 2001 Only protein-protein interactions imex curation bms1_yeast Ribosome biogenesis protein BMS1 YPL217C BMS1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MEQSNKQHRKAKEKNTAKKKLHTQGHNAKAFAVAAPGKMARTMQRSSDVNERKLHVPMVDRTPEDDPPPFIVAVVGPPGTGKTTLIRSLVRRMTKSTLNDIQGPITVVSGKHRRLTFLECPADDLNAMIDIAKIADLVLLLIDGNFGFEMETMEFLNIAQHHGMPRVLGVATHLDLFKSQSTLRASKKRLKHRFWTEVYQGAKLFYLSGVINGRYPDREILNLSRFISVMKFRPLKWRNEHPYMLADRFTDLTHPELIETQGLQIDRKVAIYGYLHGTPLPSAPGTRVHIAGVGDFSVAQIEKLPDPCPTPFYQQKLDDFEREKMKEEAKANGEITTASTTRRRKRLDDKDKLIYAPMSDVGGVLMDKDAVYIDIGKKNEEPSFVPGQERGEGEKLMTGLQSVEQSIAEKFDGVGLQLFSNGTELHEVADHEGMDVESGEESIEDDEGKSKGRTSLRKPRIYGKPVQEEDADIDNLPSDEEPYTNDDDVQDSEPRMVEIDFNNTGEQGAEKLALETDSEFEESEDEFSWERTAANKLKKTESKKRTWNIGKLIYMDNISPEECIRRWRGEDDDSKDESDIEEDVDDDFFRKKDGTVTKEGNKDHAVDLEKFVPYFDTFEKLAKKWKSVDAIKERFLGAGILGNDNKTKSDSNEGGEELYGDFEDLEDGNPSEQAEDNSDKESEDEDENEDTNGDDDNSFTNFDAEEKKDLTMEQEREMNAAKKEKLRAQFEIEEGENFKEDDENNEYDTWYELQKAKISKQLEINNIEYQEMTPEQRQRIEGFKAGSYVRIVFEKVPMEFVKNFNPKFPIVMGGLLPTEIKFGIVKARLRRHRWHKKILKTNDPLVLSLGWRRFQTLPIYTTTDSRTRTRMLKYTPEHTYCNAAFYGPLCSPNTPFCGVQIVANSDTGNGFRIAATGIVEEIDVNIEIVKKLKLVGFPYKIFKNTAFIKDMFSSAMEVARFEGAQIKTVSGIRGEIKRALSKPEGHYRAAFEDKILMSDIVILRSWYPVRVKKFYNPVTSLLLKEKTEWKGLRLTGQIRAAMNLETPSNPDSAYHKIERVERHFNGLKVPKAVQKELPFKSQIHQMKPQKKKTYMAKRAVVLGGDEKKARSFIQKVLTISKAKDSKRKEQKASQRKERLKKLAKMEEEKSQRDKEKKKEYFAQNGKRTTMGGDDESRPRKMRR 9A337F1EE0B0F21D 9A337F1EE0B0F21D rcl1_yeast RNA 3'-terminal phosphate cyclase-like protein RCL1 RTC1 YOL010W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSSAPKYTTFQGSQNFRLRIVLATLSGKPIKIEKIRSGDLNPGLKDYEVSFLRLIESVTNGSVIEISYTGTTVIYRPGIIVGGASTHICPSSKPVGYFVEPMLYLAPFSKKKFSILFKGITASHNDAGIEAIKWGLMPVMEKFGVRECALHTLKRGSPPLGGGEVHLVVDSLIAQPITMHEIDRPIISSITGVAYSTRVSPSLVNRMIDGAKKVLKNLQCEVNITADVWRGENSGKSPGWGITLVAQSKQKGWSYFAEDIGDAGSIPEELGEKVACQLLEEISKSAAVGRNQLPLAIVYMVIGKEDIGRLRINKEQIDERFIILLRDIKKIFNTEVFLKPVDEADNEDMIATIKGIGFTNTSKKIA C5DDA7F9B0C1E296 C5DDA7F9B0C1E296 bms1-rcl1 1 4 unspecified role unspecified role bait bait c-terminal protein a tag protein a tag protein A protein A c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role prey prey association association Figure 1a Bms1p-ProtA immunoprecipitates Rcl1p. bms1-rcl1-1 2 4 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role bait bait physical association physical association Of these, 31 contained prey plasmids harboring fragments of the same ORF, YPL217C (hereafter referred to as BMS1), fused in-frame with the GAL4 activation domain. bms-rcl1 3 5 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component colocalization colocalization Figure 6a Bms1p and Rcl1p cosediment together in the 10S complex and in larger structures representing nascent ribosomes.