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        <shortLabel>IntAct</shortLabel>
        <fullName>European Bioinformatics Institute</fullName>
        <alias type="synonym" typeAc="MI:1041">IntAct</alias>
        <alias type="synonym" typeAc="MI:1041">intact</alias>
      </names>
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        <attribute name="id-validation-regexp" nameAc="MI:0628">EBI-[0-9]+|IA:[0-9]+</attribute>
        <attribute name="definition">INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions.</attribute>
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        <attribute name="postaladdress">European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact/</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Identification of a possible MAP kinase cascade in Arabidopsis thaliana based on pairwise yeast two-hybrid analysis and functional complementation tests of yeast mutants.</fullName>
        </names>
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          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Identification of a possible MAP kinase cascade in Arabidopsis thaliana based on pairwise yeast two-hybrid analysis and functional complementation tests of yeast mutants.</attribute>
            <attribute name="journal" nameAc="MI:0885">FEBS Lett. (0014-5793)</attribute>
            <attribute name="publication year" nameAc="MI:0886">1998</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Mizoguchi T., Ichimura K., Irie K., Morris P., Giraudat J., Matsumoto K., Shinozaki K.</attribute>
            <attribute name="contact-email" nameAc="MI:0634">sinozaki@rtc.riken.go.jp</attribute>
            <attribute name="author-announcement">14-Nov-2008: Contacted by JYOTI.</attribute>
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            <attribute name="imex curation" nameAc="MI:0959"/>
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        <hostOrganismList>
          <hostOrganism ncbiTaxId="4932">
            <names>
              <shortLabel>yeasx</shortLabel>
              <fullName>Saccharomyces cerevisiae (Baker's yeast)</fullName>
              <alias type="synonym" typeAc="MI:1041">Baker's yeast</alias>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>2 hybrid</shortLabel>
            <fullName>two hybrid</fullName>
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            <alias type="go synonym" typeAc="MI:0303">2h</alias>
            <alias type="go synonym" typeAc="MI:0303">Gal4 transcription regeneration</alias>
            <alias type="go synonym" typeAc="MI:0303">two-hybrid</alias>
            <alias type="go synonym" typeAc="MI:0303">yeast two hybrid</alias>
            <alias type="go synonym" typeAc="MI:0303">2-hybrid</alias>
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            <alias type="synonym" typeAc="MI:1041">Gal4 transcription regeneration</alias>
            <alias type="synonym" typeAc="MI:1041">2h</alias>
            <alias type="synonym" typeAc="MI:1041">yeast two hybrid</alias>
            <alias type="synonym" typeAc="MI:1041">Y2H</alias>
            <alias type="synonym" typeAc="MI:1041">Y-2H</alias>
            <alias type="synonym" typeAc="MI:1041">2 hybrid</alias>
          </names>
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        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
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        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Mizoguchi T., Ichimura K., Irie K., Morris P., Giraudat J., Matsumoto K., Shinozaki K.</attribute>
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          <attribute name="accepted">Accepted 2008-NOV-20 by JYOTI.</attribute>
          <attribute name="journal" nameAc="MI:0885">FEBS Lett. (0014-5793)</attribute>
          <attribute name="publication year" nameAc="MI:0886">1998</attribute>
          <attribute name="reviewer">jyoti</attribute>
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          <attribute name="imex curation" nameAc="MI:0959"/>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
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      </experimentDescription>
    </experimentList>
    <interactorList>
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        <names>
          <shortLabel>mpk4_arath</shortLabel>
          <fullName>Mitogen-activated protein kinase 4</fullName>
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          <alias type="locus name" typeAc="MI:0305">At4g01370</alias>
          <alias type="orf name" typeAc="MI:0306">F2N1.1</alias>
          <alias type="orf name" typeAc="MI:0306">F2N1_2-t</alias>
        </names>
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          <names>
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            <fullName>protein</fullName>
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          <names>
            <shortLabel>arath</shortLabel>
            <fullName>Arabidopsis thaliana (Mouse-ear cress)</fullName>
            <alias type="synonym" typeAc="MI:1041">Mouse-ear cress</alias>
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        <sequence>MSAESCFGSSGDQSSSKGVATHGGSYVQYNVYGNLFEVSRKYVPPLRPIGRGAYGIVCAATNSETGEEVAIKKIGNAFDNIIDAKRTLREIKLLKHMDHENVIAVKDIIKPPQRENFNDVYIVYELMDTDLHQIIRSNQPLTDDHCRFFLYQLLRGLKYVHSANVLHRDLKPSNLLLNANCDLKLGDFGLARTKSETDFMTEYVVTRWYRAPELLLNCSEYTAAIDIWSVGCILGETMTREPLFPGKDYVHQLRLITELIGSPDDSSLGFLRSDNARRYVRQLPQYPRQNFAARFPNMSAGAVDLLEKMLVFDPSRRITVDEALCHPYLAPLHDINEEPVCVRPFNFDFEQPTLTEENIKELIYRETVKFNPQDSV</sequence>
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      </interactor>
      <interactor id="3">
        <names>
          <shortLabel>m3k1_arath</shortLabel>
          <fullName>Mitogen-activated protein kinase kinase kinase 1</fullName>
          <alias type="gene name" typeAc="MI:0301">MEKK1</alias>
          <alias type="locus name" typeAc="MI:0305">At4g08500</alias>
          <alias type="orf name" typeAc="MI:0306">T15F16.5</alias>
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        <sequence>MDRILARMKKSTGRRGGDKNITPVRRLERRDAARNINYDAASCSSSSAEDLSVSTSSLMTRSLEFPEPTSFRIGGGVGEMDRIYRSLGVSGPDDLAISFDAWEACKKRSSSDVVNRFKSFDLDKVRDQDLSEEGPSGVVVGSDSMNHKVQGQDLSEAGPSGGIVTELSEIGNLITPVDRLVADGVVENRRVMERTPTIVKSKGYLVPNNVVAVGVGVGGGIKGLRPPVLKPPPAMKRPPIDHRGSSWDFLTHFAPSETVKRPSSSSSSSEDGCDEEEGKEEEAEAEEMGARFIQLGDTADETCSFTTNEGDSSSTVSNTSPIYPDGGAIITSWQKGQLLGRGSFGSVYEGISGDGDFFAVKEVSLLDQGSQAQECIQQLEGEIKLLSQLQHQNIVRYRGTAKDGSNLYIFLELVTQGSLLKLYQRYQLRDSVVSLYTRQILDGLKYLHDKGFIHRDIKCANILVDANGAVKLADFGLAKVSKFNDIKSCKGTPFWMAPEVINRKDSDGYGSPADIWSLGCTVLEMCTGQIPYSDLEPVQALFRIGRGTLPEVPDTLSLDARLFILKCLKVNPEERPTAAELLNHPFVRRPLPSVGSGGSGSASPLLRR</sequence>
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          <shortLabel>m2k1_arath</shortLabel>
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          <alias type="gene name synonym" typeAc="MI:0302">NMAPKK</alias>
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          <alias type="gene name" typeAc="MI:0301">MKK1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">MEK1</alias>
          <alias type="locus name" typeAc="MI:0305">At4g26070</alias>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0042542" version="SP_153"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0042742" version="SP_153"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0045087" version="SP_153"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0098542" version="SP_153"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0106310" version="SP_153"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0106311" version="SP_153"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_194337.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_849446.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_974619.1"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR011009"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR000719"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR017441"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR008271"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="3702">
          <names>
            <shortLabel>arath</shortLabel>
            <fullName>Arabidopsis thaliana (Mouse-ear cress)</fullName>
            <alias type="synonym" typeAc="MI:1041">Mouse-ear cress</alias>
          </names>
        </organism>
        <sequence>MNRGSLCPNPICLPPLEQSISKFLTQSGTFKDGDLRVNKDGIQTVSLSEPGAPPPIEPLDNQLSLADLEVIKVIGKGSSGNVQLVKHKLTQQFFALKVIQLNTEESTCRAISQELRINLSSQCPYLVSCYQSFYHNGLVSIILEFMDGGSLADLLKKVGKVPENMLSAICKRVLRGLCYIHHERRIIHRDLKPSNLLINHRGEVKITDFGVSKILTSTSSLANSFVGTYPYMSPERISGSLYSNKSDIWSLGLVLLECATGKFPYTPPEHKKGWSSVYELVDAIVENPPPCAPSNLFSPEFCSFISQCVQKDPRDRKSAKELLEHKFVKMFEDSDTNLSAYFTDAGSLIPPLAN</sequence>
        <attributeList>
          <attribute name="crc64">D889CFBB97653B8E</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="5" imexId="IM-20122-1">
        <names>
          <shortLabel>mek1-mpk4</shortLabel>
        </names>
        <xref>
          <primaryRef db="intact" dbAc="MI:0469" id="EBI-2011364" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-20122-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="6">
            <interactorRef>4</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>bait</shortLabel>
                  <fullName>bait</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
          <participant id="7">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>prey</shortLabel>
                  <fullName>prey</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">Table 1</attribute>
          <attribute name="source-text">As shown in Table 1, interaction between ATMPK4 and MEK1 was detected, based on both growth on His-lacking medium and &amp;#946;-galactosidase activity</attribute>
        </attributeList>
      </interaction>
      <interaction id="8" imexId="IM-20122-2">
        <names>
          <shortLabel>mkk1-mek1</shortLabel>
        </names>
        <xref>
          <primaryRef db="intact" dbAc="MI:0469" id="EBI-2011374" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-20122-2" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="9">
            <interactorRef>4</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>prey</shortLabel>
                  <fullName>prey</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
          <participant id="10">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>bait</shortLabel>
                  <fullName>bait</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">Table 1</attribute>
          <attribute name="source-text">Interaction between ATMEKK1 and MEK1 was detected, based on both growth on His-lacking medium and &amp;#946;-galactosidase activity</attribute>
        </attributeList>
      </interaction>
      <interaction id="11" imexId="IM-20122-3">
        <names>
          <shortLabel>mek1-mkk1</shortLabel>
        </names>
        <xref>
          <primaryRef db="intact" dbAc="MI:0469" id="EBI-2011379" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-20122-3" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="12">
            <interactorRef>4</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>bait</shortLabel>
                  <fullName>bait</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
          <participant id="13">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>prey</shortLabel>
                  <fullName>prey</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">Table 1</attribute>
          <attribute name="source-text">Interaction between ATMEKK1 and MEK1 was detected, based on both growth on His-lacking medium and &amp;#946;-galactosidase activity</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>