UniProt UniProt http://www.uniprot.org Interaction of Hic-5, A senescence-related protein, with focal adhesion kinase. Interaction of Hic-5, A senescence-related protein, with focal adhesion kinase. J. Biol. Chem. (0021-9258) 1998 Fujita H., Kamiguchi K., Cho D., Shibanuma M., Morimoto C., Tachibana K. COS1 cells have been co-transfected with Tgfb1i1/Hic-5 and PTK2/FAK plasmids. tachiban@mbcrr.harvard.edu 14-Nov-2008: Contacted by JYOTI. orchard chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Fujita H., Kamiguchi K., Cho D., Shibanuma M., Morimoto C., Tachibana K. COS1 cells have been co-transfected with Tgfb1i1/Hic-5 and PTK2/FAK plasmids. tachiban@mbcrr.harvard.edu Accepted 2008-OCT-22 by ORCHARD. J. Biol. Chem. (0021-9258) 1998 orchard Interaction of Hic-5, A senescence-related protein, with focal adhesion kinase. Interaction of Hic-5, A senescence-related protein, with focal adhesion kinase. J. Biol. Chem. (0021-9258) 1998 Fujita H., Kamiguchi K., Cho D., Shibanuma M., Morimoto C., Tachibana K. COS1 cells have been co-transfected with Tgfb1i1/Hic-5 and PTK2/FAK plasmids. tachiban@mbcrr.harvard.edu 14-Nov-2008: Contacted by JYOTI. orchard in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Fujita H., Kamiguchi K., Cho D., Shibanuma M., Morimoto C., Tachibana K. COS1 cells have been co-transfected with Tgfb1i1/Hic-5 and PTK2/FAK plasmids. tachiban@mbcrr.harvard.edu Accepted 2008-OCT-22 by ORCHARD. 14-Nov-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 1998 orchard Interaction of Hic-5, A senescence-related protein, with focal adhesion kinase. Interaction of Hic-5, A senescence-related protein, with focal adhesion kinase. J. Biol. Chem. (0021-9258) 1998 Fujita H., Kamiguchi K., Cho D., Shibanuma M., Morimoto C., Tachibana K. COS1 cells have been co-transfected with Tgfb1i1/Hic-5 and PTK2/FAK plasmids. tachiban@mbcrr.harvard.edu 14-Nov-2008: Contacted by JYOTI. orchard human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip antibody detection identification by antibody antibody detection Fujita H., Kamiguchi K., Cho D., Shibanuma M., Morimoto C., Tachibana K. COS1 cells have been co-transfected with Tgfb1i1/Hic-5 and PTK2/FAK plasmids. tachiban@mbcrr.harvard.edu Accepted 2008-OCT-22 by ORCHARD. 14-Nov-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 1998 orchard paxi_human Paxillin PXN protein protein human Homo sapiens Human MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTILDPLDQWQPSSSRFIHQQPQSSSPVYGSSAKTSSVSNPQDSVGSPCSRVGEEEHVYSFPNKQKSAEPSPTVMSTSLGSNLSELDRLLLELNAVQHNPPGFPADEANSSPPLPGALSPLYGVPETNSPLGGKAGPLTKEKPKRNGGRGLEDVRPSVESLLDELESSVPSPVPAITVNQGEMSSPQRVTSTQQQTRISASSATRELDELMASLSDFKIQGLEQRADGERCWAAGWPRDGGRSSPGGQDEGGFMAQGKTGSSSPPGGPPKPGSQLDSMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHNLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCGAFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLWHPECFVCRECFTPFVNGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRCITAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQNCFLKLFC DIP protein P49023 original sequence version: 115 DIP protein P49023 original sequence version: 126 DIP protein P49023 original sequence version: 169 DIP protein P49023 original sequence version: 140 DIP protein P49023 original sequence version: 175 ABF6C0BE5939623F fak1_human Focal adhesion kinase 1 PTK2 FAK FAK1 pp125FAK Protein-tyrosine kinase 2 Focal adhesion kinase-related nonkinase Protein phosphatase 1 regulatory subunit 71 p125FAK protein protein human Homo sapiens Human MAAAYLDPNLNHTPNSSTKTHLGTGMERSPGAMERVLKVFHYFESNSEPTTWASIIRHGDATDVRGIIQKIVDSHKVKHVACYGFRLSHLRSEEVHWLHVDMGVSSVREKYELAHPPEEWKYELRIRYLPKGFLNQFTEDKPTLNFFYQQVKSDYMLEIADQVDQEIALKLGCLEIRRSYWEMRGNALEKKSNYEVLEKDVGLKRFFPKSLLDSVKAKTLRKLIQQTFRQFANLNREESILKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGISYLTDKGCNPTHLADFTQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVNGTSQSFIIRPQKEGERALPSIPKLANSEKQGMRTHAVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRERIELGRCIGEGQFGDVHQGIYMSPENPALAVAIKTCKNCTSDSVREKFLQEALTMRQFDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQVRKYSLDLASLILYAYQLSTALAYLESKRFVHRDIAARNVLVSSNDCVKLGDFGLSRYMEDSTYYKASKGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILEEEKAQQEERMRMESRRQATVSWDSGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQPVGKPDPAAPPKKPPRPGAPGHLGSLASLSSPADSYNEGVKLQPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDETIPLLPASTHREIEMAQKLLNSDLGELINKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMLGQTRPH D8A4C15138AB0243 tgfi1_human Transforming growth factor beta-1-induced transcript 1 protein TGFB1I1 ARA55 Hydrogen peroxide-inducible clone 5 protein Androgen receptor-associated protein of 55 kDa Androgen receptor coactivator 55 kDa protein protein protein human Homo sapiens Human MEDLDALLSDLETTTSHMPRSGAPKERPAEPLTPPPSYGHQPQTGSGESSGASGDKDHLYSTVCKPRSPKPAAPAAPPFSSSSGVLGTGLCELDRLLQELNATQFNITDEIMSQFPSSKVASGEQKEDQSEDKKRPSLPSSPSPGLPKASATSATLELDRLMASLSDFRVQNHLPASGPTQPPVVSSTNEGSPSPPEPTGKGSLDTMLGLLQSDLSRRGVPTQAKGLCGSCNKPIAGQVVTALGRAWHPEHFVCGGCSTALGGSSFFEKDGAPFCPECYFERFSPRCGFCNQPIRHKMVTALGTHWHPEHFCCVSCGEPFGDEGFHEREGRPYCRRDFLQLFAPRCQGCQGPILDNYISALSALWHPDCFVCRECFAPFSGGSFFEHEGRPLCENHFHARRGSLCATCGLPVTGRCVSALGRRFHPDHFTCTFCLRPLTKGSFQERAGKPYCQPCFLKLFG D2C7C32C3FD2C496 tgfi1_mouse Transforming growth factor beta-1-induced transcript 1 protein Tgfb1i1 Ara55 Hydrogen peroxide-inducible clone 5 protein Androgen receptor-associated protein of 55 kDa TGF beta-stimulated clone 5 protein protein mouse Mus musculus Mouse MEDLDALLSDLETTTSHMSRLGAPKERPPETLTPPPPYGHQPQTGSGESSGTTGDKDHLYSTVCKPRSPKPVAPVAPPFSSSSGVLGNGLCELDRLLQELNATQFNITDEIMSQFPSSKMAEGEEKEDQSEDKSSPTVPPSPFPAPSKPSATSATQELDRLMASLSDFRVQNHLPASGPPQPPAASPTREGCPSPPGQTSKGSLDTMLGLLQSDLSRRGVPTQAKGLCGSCNKPIAGQVVTALGRAWHPEHFLCSGCSTTLGGSSFFEKDGAPFCPECYFERFSPRCGFCNQPIRHKMVTALGTHWHPEHFCCVSCGEPFGEEGFHEREGRPYCRRDFLQLFAPRCQGCQGPILDNYISALSALWHPDCFVCRECLAPFSGGSFFEHEGRPLCENHFHAQRGSLCATCGLPVTGRCVSALGRRFHPDHFTCTFCLRPLTKGSFQERASKPYCQPCFLKLFG 6AB15FFF466FEE73 tgfb1i1-ptk2 1 7 unspecified role unspecified role bait bait c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 5 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 physical association physical association 1A co-immunoprecipitation of FAK with Hic-5 pxn-ptk2-1 1 4 unspecified role unspecified role bait bait c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 5 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 physical association physical association 1A Paxillin binds to the C-terminal domain of FAK by its N-terminal half. pxn-ptk2-2 2 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q05397:p.Leu1034Ser mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L S Wed May 02 14:28:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu1034ser' Tue Jun 30 16:42:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1034Ser' Q05397:p.Val928Gly mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain V G Wed May 02 17:57:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val928gly' Tue Jun 30 19:06:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val928Gly' region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 physical association physical association 1C both Hic-5 and paxillin were precipitated with GST-FAK (903-1052) and its substitution mutants, K933E and Q1040G. However, neither protein were precipitated with GST and GST-FAK mutants, V928G and L1034S. tgfb1i1-ptk2-1 2 5 unspecified role unspecified role bait bait Q05397:p.Val928Gly mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain V G Wed May 02 17:48:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val928gly' Tue Jun 30 16:45:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val928Gly' region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q05397:p.Leu1034Ser mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L S Wed May 02 18:24:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu1034ser' Tue Jun 30 15:41:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1034Ser' ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 physical association physical association 1C both Hic-5 and paxillin were precipitated with GST-FAK (903-1052) and its substitution mutants, K933E and Q1040G. However, neither protein were precipitated with GST and GST-FAK mutants, V928G and L1034S. tgfb1i1-ptk2-2 3 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association 2B As shown in Fig. 2B, endogenous FAK was coprecipiated with endogenous Hic-5, indicating the FAK-Hic-5 interaction in vivo tgfb1i1-ptk2-3 2 7 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 1B As shown in Fig. 1B, the wild-type and N-terminal half of Hic-5 were precipitated with GST-FAK-CT, whereas the C-terminal half of Hic-5 was not precipitated with GST-FAK-CT.