UniProt UniProt http://www.uniprot.org Functional dissection of yeast Hir1p, a WD repeat-containing transcriptional corepressor. Functional dissection of yeast Hir1p, a WD repeat-containing transcriptional corepressor. Genetics 148 (657-667) 1998 imex curation DeSilva H., Lee K., Osley MA. Experiement was performed in a protease-defiscient strain to reduce non-specific proteolysis during extract preparation. m-osley@ski.mskcc.org 03-Mar-2009: Contacted by JYOTI. 03-Feb-2009: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western DeSilva H., Lee K., Osley MA. Experiement was performed in a protease-defiscient strain to reduce non-specific proteolysis during extract preparation. m-osley@ski.mskcc.org Accepted 2009-FEB-22 by ORCHARD. 03-Mar-2009: Contacted by JYOTI. Genetics 148 (657-667) 1998 orchard Only protein-protein interactions imex curation spt4_yeast Transcription elongation factor SPT4 YGR063C SPT4 Chromatin elongation factor SPT4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSERACMLCGIVQTTNEFNRDGCPNCQGIFEEAGVSTMECTSPSFEGLVGMCKPTKSWVAKWLSVDHSIAGMYAIKVDGRLPAEVVELLPHYKPRDGSQVE 59C164193ABA883B 59C164193ABA883B hir1_yeast Protein HIR1 HIR1 YBL008W YBL0318 Histone transcription regulator 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKVVKFPWLAHREESRKYEIYTVDVSHDGKRLATGGLDGKIRIWSIDSILRCMELESLTPEIPLPQDLQMPLCSMSRHTGSITCVKFSPDGKYLASGSDDRILLIWALDEEQSSQPAFGSEHEREHWTVRKRLVAHDNDIQDICWAPDSSILVTVGLDRSVIVWNGSTFEKLKRFDVHQSLVKGVVFDPANKYFATTSDDRTMKIFRYHKTGDISFTIEHIITEPFKESPLTTYFRRPSWSPDGQHIAVPNATNGPVSSVAIVNRGTWDTNVSLIGHDAPTEVARFNPRLFERNAGVKQKKDDDPENALVGQNDDKVHHFDKNIDSVVATAGQDKSLAVWSTSRPRPILVAFDIANKSITDMSWNPDGSLLFVASLDSSITLFKFENNELGKPIPLEKNMEQLYRYGVDKDSLDFPESINQLLLEDQTKSFKHTKISTSKLGENHPTLATNSASNQKDNNDASVSRSEHINILIPKRKKDAILNKAVTLKSGKKRVAPTLISTSSSSPFSNGIKKPTLDSKRIENNVKSSTKTINSKNTLLNVPEGVEKKISISSFPLPRLGIHSLIMGTKERSAWKISNSELENDDADNAGGKGSDGTSNSIDDIAVLSEEENDFHRMTLNAKLTQEKIWSEEPTTRCLLQSDVIPDTDVVVLEGGSLDDIAVLEIRNGVERSIQFDSEALLDNPTRILGYQGGKRTIETFIPEVIICAIGSKDCKCWCLASANGSIYILSYNGQQRIPKICLGHKVIKMVTSSKYLLVLTERGLFFAWDLLDLKLVLRNVPILPILNGQPIHGNKVRINKVIKCFRLDGSSCDLLLEVGDPKNVYKWTKDLGCWSLYK 40A2932F3306358A 40A2932F3306358A hir2_yeast Protein HIR2 HIR2 YOR038C OR26.31 Histone transcription regulator 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MRLLKYPLDIHNEQVNALAALGPYIILAGSGGHVMAWRQQQLVDTAFDRVMIKDLKPEVSFQVDQDTTGDIFFITGDLETLYIGSEHRLWGYSGWLCRDTNNINSVEKMNSKLLFECKSPSTITDVKYDINLGILFVLLSNENKILLFRHKTFDKLSEITIDKASKPITGIIDPTGQTFTVMTSDRSILVYQINKTGTHKLINKLTQHVQMYPLHYRISMSPQADILPVINSVKGVPNNATSCTALLDRNNNYKVTKTLVTPSSNGCRVLVYSPAFYEKPNLKKGTSTRYNLIATSGSTDGTILVWNTKRMKPLFNALQVSSTAINDMSWSQDGFTLFAISNDATLYTFAFQEKDLGVALPQTEIKSLQEVNKKLPKLEEPLAEQIPKSFPENIKLEESASAAPIPNDIGRSAVGKKPTKKKTANNQTNGIKTIQSTSMEFNTPSYTVPRDLKRKPKEATPSNIAPGSKKQKKELQPIDFLDTGLLLPNTSFSRIRLATPKIRSTFKYSPINNPNLILDVKNGSGNEQRPTIVKLTSKVLDQDQVLFQDFIPKLITICTAGDTFWSFCSEDGSIYIYSDSGRKLMAPLVLGVSISFLEACGTYLLCLTSIGELYCWNIEQKKLAFPTNTIYPLLNPSLRYSDDILTRAENITLCSITKKGVPLVTLSNGDGYLFDKNMETWLLVSDGWWAYGSQYWDTTNTTGLSSSKANTDSFNGSESNINEIVSDIKNDNQSIINFLECKTNDELNRKGRIKNLQRFARTILMKEGFENMEEIVTLSHLENKILISIRLEEPEEFSKLMMVYCIRLSELGYMDRLNDVFQWLYDDLPISGTGSAFADKDFKRNLLKKILIACGDIRQVQRVTTRYAKEMNIIS C77D677BF424680D C77D677BF424680D hir1-hir1-1 1 3 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey n-terminus 7 WD repeats region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal LexA fusion protein fusion protein fusion protein n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 2A Both C-termnus and N-terminus binding sites are sufficient for the interaction. The results showed that both the Hir1p WD repeats and C terminus could be coprecipitated with fulllength HA-Hir1p hir1-hir2-2 1 4 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey c-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain n-terminal LexA fusion protein fusion protein fusion protein n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 2B In contrast, only the LexA-Hir1(Cterm) fusion protein was coprecipitated with HA-Hir2p (Figure 2B, lane 6), spt4-hir1 1 3 unspecified role unspecified role prey prey n-terminal LexA fusion protein fusion protein fusion protein n-term range n-terminal range n-term range n-term range n-terminal range n-term range 2 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 6A Coprecipitation of Spt4p and Hir1p from yeast cell extracts. spt4-hir1-1 1 3 unspecified role unspecified role prey prey region LexA fusion protein ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 2 unspecified role unspecified role bait bait n-terminal LexA fusion protein fusion protein fusion protein n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 6B Coprecipitation of Spt4p and Hir1p from yeast cell extracts.