IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Response regulators implicated in His-to-Asp phosphotransfer signaling in Arabidopsis. Response regulators implicated in His-to-Asp phosphotransfer signaling in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1998 Imamura A., Hanaki N., Umeda H., Nakamura A., Suzuki T., Ueguchi C., Mizuno T. Proteins and preparation methods were identified from references provided in the text. i45455a@nucc.cc.nagoya-u.ac.jp 27-Aug-2008: Contacted by JYOTI. in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Imamura A., Hanaki N., Umeda H., Nakamura A., Suzuki T., Ueguchi C., Mizuno T. Proteins and preparation methods were identified from references provided in the text. i45455a@nucc.cc.nagoya-u.ac.jp Accepted 2008-AUG-14 by CDEROW. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1998 a0a0h2vbi7_ecol6 Aerobic respiration control sensor protein arcB c3970 protein protein ecol6 Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTMVLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDLSLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSFLDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEKVFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDALERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNIFNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQVITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKIFAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSVAEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFKPGEYDLVLLDIQLPDMTGLDISRALTKRYPREDLPPLVALTANVLKDKQEYLNAGMDDVLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLITDGLAVFERMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQSPDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK 83ABBBE115D8AFE4 arr3_arath Two-component response regulator ARR3 ARR3 F23H11.25 At1g59940 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAKDGGVSCLRRSEMIGIGIGELESPPLDSDQVHVLAVDDSLVDRIVIERLLRITSCKVTAVDSGWRALEFLGLDDDKAAVEFDRLKVDLIITDYCMPGMTGYELLKKIKESTSFKEVPVVIMSSENVMTRIDRCLEEGAEDFLLKPVKLADVKRLRSYLTRDVKVAAEGNKRKLTTPPPPPPLSATSSMESSDSTVESPLSMVDDEDSLTMSPESATSLVDSPMRSPGLA CD4950AE87FAFC91 arcb-arr3 1 2 enzyme enzyme neutral component neutral component 3 enzyme target enzyme target substrate substrate neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain phosphorylation phosphorylation reaction phosphorylation Figure 3C phosphoryllation of Q9ZWS9 Based on this example, we determined whether the purified ARR polypeptides (designated as ARR-H6) can acquire the phosphoryl group from a labeled phospho-HPt domain in a purified system The authors proposed "the phosphoryl group on the HPt domain was transferred rapidly to an aspartate residue of ARR-H6, and then the phosphoryl group was released from ARR-H6". Q8FD66 was isolated using anion exchange chromatography and fast protein liquid chromatography. It is not certain that the resulting purified samples contains only Q8FD66.