IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The protein-folding activity of chaperonins correlates with the symmetric GroEL14(GroES7)2 heterooligomer. The protein-folding activity of chaperonins correlates with the symmetric GroEL14(GroES7)2 heterooligomer. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1995 imex curation Azem A., Diamant S., Kessel M., Weiss C., Goloubinoff P. Due to the better resolution on SDS gels of the intermediate crosslinking species GroEL7 and GroEL7GroES7, as compared to the poor resolution of the final crosslinking products of GroEL14GroES7 and GroEL14(GroES7)2, the fraction of GroEL14GroES7 and GroEL14(GroES7)2 particles in a chaperonin solution was determined from the ratio of crosslinked species GroEL7 and GroEL7GroES7. orchard Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight by staining molecular weight estimation by staining weight by staining Azem A., Diamant S., Kessel M., Weiss C., Goloubinoff P. Due to the better resolution on SDS gels of the intermediate crosslinking species GroEL7 and GroEL7GroES7, as compared to the poor resolution of the final crosslinking products of GroEL14GroES7 and GroEL14(GroES7)2, the fraction of GroEL14GroES7 and GroEL14(GroES7)2 particles in a chaperonin solution was determined from the ratio of crosslinked species GroEL7 and GroEL7GroES7. Due to the better resolution on SDS gels of the intermediate crosslinking species GroEL7 and GroEL7GroES7, as compared to the poor resolution of the final crosslinking products of GroEL14GroES7 and GroEL14(GroES7)2, the fraction of GroEL14GroES7 and GroEL14(GroES7)2 particles in a chaperonin solution was determined from the ratio of crosslinked species GroEL7 and GroEL7GroES7. Accepted 2008-Not a month!-10 by ORCHARD. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1995 orchard Only protein-protein interactions imex curation ch10_ecoli Co-chaperonin GroES groES 10 kDa chaperonin Chaperonin-10 groS mopB JW4102 b4142 protein protein ecoli Escherichia coli (strain K12) MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVGNGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA 76829E09B11217EF 76829E09B11217EF ch60_ecoli Chaperonin GroEL groEL 60 kDa chaperonin Chaperonin-60 GroEL protein groL mopA JW4103 b4143 protein protein ecoli Escherichia coli (strain K12) MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM CD3A0FB505F74AD1 CD3A0FB505F74AD1 groes-groel 1 3 unspecified role unspecified role neutral component neutral component 2 unspecified role unspecified role neutral component neutral component association association 1A, B and Table 1 Oligomers formed. amount of crosslinked GroEL7 and GroEL7GroES7 species and, in contrast, a native GroEL14(GroES7)2 particle produces only GroEL7GroES7, with no GroEL7 crosslinked species (Table 1, Fig. 1A). groel-groel 1 3 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component association association 1A, B and Table 1 Oligomers formed. amount of crosslinked GroEL7 and GroEL7GroES7 species and, in contrast, a native GroEL14(GroES7)2 particle produces only GroEL7GroES7, with no GroEL7 crosslinked species (Table 1, Fig. 1A). groes-groel-1 1 3 unspecified role unspecified role neutral component neutral component 2 unspecified role unspecified role neutral component neutral component association association 1A, B, 3 and Table 1 Oligomers formed. amount of crosslinked GroEL7 and GroEL7GroES7 species and, in contrast, a native GroEL14(GroES7)2 particle produces only GroEL7GroES7, with no GroEL7 crosslinked species (Table 1, Fig. 1A). ATP regeneration system gives more samples in a 2:1 ratio of gro ES to GRO EL. The ADP acts as a uncoupling agent.