IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Arabidopsis COP1 protein specifically interacts in vitro with a cytoskeleton-associated protein, CIP1. Arabidopsis COP1 protein specifically interacts in vitro with a cytoskeleton-associated protein, CIP1. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1995 imex curation Matsui M., Stoop CD., von Arnim AG., Wei N., Deng XW. jyoti Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting weight by staining molecular weight estimation by staining weight by staining Matsui M., Stoop CD., von Arnim AG., Wei N., Deng XW. Accepted 2009-JUL-8 by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1995 jyoti Only protein-protein interactions imex curation Arabidopsis COP1 protein specifically interacts in vitro with a cytoskeleton-associated protein, CIP1. Arabidopsis COP1 protein specifically interacts in vitro with a cytoskeleton-associated protein, CIP1. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1995 imex curation Matsui M., Stoop CD., von Arnim AG., Wei N., Deng XW. jyoti Only protein-protein interactions in vitro In vitro lambda phage lambda phage display lambda phage nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Matsui M., Stoop CD., von Arnim AG., Wei N., Deng XW. Accepted 2009-JUL-8 by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1995 jyoti Only protein-protein interactions imex curation cip1_arath COP1-interactive protein 1 CIP1 K16L22.7 At5g41790 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKKHKFRETLKSFFEPHFDHEKGEMLKGTKTEIDEKVNKILGMVESGDVNEDESNRQVVADLVKEFYSEYQSLYRQYDDLTGEIRKKVNGKGESSSSSSSDSDSDHSSKRKVKRNGNGKVEKDVELVTGALKQQIEAANLEIADLKGKLTTTVEEKEAVDSELELALMKLKESEEISSKLKLETEKLEDEKSIALSDNRELHQKLEVAGKTETDLNQKLEDIKKERDELQTERDNGIKRFQEAEKVAEDWKTTSDQLKDETSNLKQQLEASEQRVSELTSGMNSAEEENKSLSLKVSEISDVIQQGQTTIQELISELGEMKEKYKEKESEHSSLVELHKTHERESSSQVKELEAHIESSEKLVADFTQSLNNAEEEKKLLSQKIAELSNEIQEAQNTMQELMSESGQLKESHSVKERELFSLRDIHEIHQRDSSTRASELEAQLESSKQQVSDLSASLKAAEEENKAISSKNVETMNKLEQTQNTIQELMAELGKLKDSHREKESELSSLVEVHETHQRDSSIHVKELEEQVESSKKLVAELNQTLNNAEEEKKVLSQKIAELSNEIKEAQNTIQELVSESGQLKESHSVKDRDLFSLRDIHETHQRESSTRVSELEAQLESSEQRISDLTVDLKDAEEENKAISSKNLEIMDKLEQAQNTIKELMDELGELKDRHKEKESELSSLVKSADQQVADMKQSLDNAEEEKKMLSQRILDISNEIQEAQKTIQEHMSESEQLKESHGVKERELTGLRDIHETHQRESSTRLSELETQLKLLEQRVVDLSASLNAAEEEKKSLSSMILEITDELKQAQSKVQELVTELAESKDTLTQKENELSSFVEVHEAHKRDSSSQVKELEARVESAEEQVKELNQNLNSSEEEKKILSQQISEMSIKIKRAESTIQELSSESERLKGSHAEKDNELFSLRDIHETHQRELSTQLRGLEAQLESSEHRVLELSESLKAAEEESRTMSTKISETSDELERTQIMVQELTADSSKLKEQLAEKESKLFLLTEKDSKSQVQIKELEATVATLELELESVRARIIDLETEIASKTTVVEQLEAQNREMVARISELEKTMEERGTELSALTQKLEDNDKQSSSSIETLTAEIDGLRAELDSMSVQKEEVEKQMVCKSEEASVKIKRLDDEVNGLRQQVASLDSQRAELEIQLEKKSEEISEYLSQITNLKEEIINKVKVHESILEEINGLSEKIKGRELELETLGKQRSELDEELRTKKEENVQMHDKINVASSEIMALTELINNLKNELDSLQVQKSETEAELEREKQEKSELSNQITDVQKALVEQEAAYNTLEEEHKQINELFKETEATLNKVTVDYKEAQRLLEERGKEVTSRDSTIGVHEETMESLRNELEMKGDEIETLMEKISNIEVKLRLSNQKLRVTEQVLTEKEEAFRKEEAKHLEEQALLEKNLTMTHETYRGMIKEIADKVNITVDGFQSMSEKLTEKQGRYEKTVMEASKILWTATNWVIERNHEKEKMNKEIEKKDEEIKKLGGKVREDEKEKEMMKETLMGLGEEKREAIRQLCVWIDHHRSRCEYLEEVLSKTVVARGQRRVSQRT FD6D862810164550 cop1_arath E3 ubiquitin-protein ligase COP1 COP1 Constitutive photomorphogenesis protein 1 RING-type E3 ubiquitin transferase COP1 T21L14.11 At2g32950 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEISTDPVVPAVKPDPRTSSVGEGANRHENDDGGSGGSEIGAPDLDKDLLCPICMQIIKDAFLTACGHSFCYMCIITHLRNKSDCPCCSQHLTNNQLYPNFLLDKLLKKTSARHVSKTASPLDQFREALQRGCDVSIKEVDNLLTLLAERKRKMEQEEAERNMQILLDFLHCLRKQKVDELNEVQTDLQYIKEDINAVERHRIDLYRARDRYSVKLRMLGDDPSTRNAWPHEKNQIGFNSNSLSIRGGNFVGNYQNKKVEGKAQGSSHGLPKKDALSGSDSQSLNQSTVSMARKKRIHAQFNDLQECYLQKRRQLADQPNSKQENDKSVVRREGYSNGLADFQSVLTTFTRYSRLRVIAEIRHGDIFHSANIVSSIEFDRDDELFATAGVSRCIKVFDFSSVVNEPADMQCPIVEMSTRSKLSCLSWNKHEKNHIASSDYEGIVTVWDVTTRQSLMEYEEHEKRAWSVDFSRTEPSMLVSGSDDCKVKVWCTRQEASVINIDMKANICCVKYNPGSSNYIAVGSADHHIHYYDLRNISQPLHVFSGHKKAVSYVKFLSNNELASASTDSTLRLWDVKDNLPVRTFRGHTNEKNFVGLTVNSEYLACGSETNEVYVYHKEITRPVTSHRFGSPDMDDAEEEAGSYFISAVCWKSDSPTMLTANSQGTIKVLVLAA DIP protein P43254 original sequence version: 138 DIP protein P43254 original sequence version: 113 DIP protein P43254 original sequence version: 114 E6D9A1B702EAE8F0 cop1-cip-5 2 3 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Arabidopsis COP1 protein specifically interacts in vitro with a cytoskeleton-associated protein, CIP1 cop1-cip-6 2 3 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Further, this same phage also interacted with biotinylated MBP-COP1 fusion protein but not with biotinylated MBP, BSA, and LacZ cop1-cip-7 1 4 unspecified role unspecified role bait bait region LacZ tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association CIP1 protein interacts specifically with the helix domai: COP1 in vitro. Figure 3