IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide. Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1991 Bischoff FR., Ponstingl H. Soluble fraction of HeLa was subject to ammonium sulphate precipitation followed by complexing with GDP; the complex so formed was purified on Sephacryl S-200. Further purification to homogenity was obtained on hydroxylapatite. in vitro In vitro reverse phase chrom reverse phase chromatography reverse phase chrom predetermined predetermined participant predetermined Bischoff FR., Ponstingl H. Soluble fraction of HeLa was subject to ammonium sulphate precipitation followed by complexing with GDP; the complex so formed was purified on Sephacryl S-200. Further purification to homogenity was obtained on hydroxylapatite. Accepted 2008-AUG-24 by CDEROW. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1991 Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide. Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1991 Bischoff FR., Ponstingl H. Soluble fraction of HeLa was subject to ammonium sulphate precipitation followed by complexing with GDP; the complex so formed was purified on Sephacryl S-200. Further purification to homogenity was obtained on hydroxylapatite. in vitro In vitro comigration in gel comigration in gel electrophoresis comigration in gel predetermined predetermined participant predetermined Bischoff FR., Ponstingl H. Soluble fraction of HeLa was subject to ammonium sulphate precipitation followed by complexing with GDP; the complex so formed was purified on Sephacryl S-200. Further purification to homogenity was obtained on hydroxylapatite. Accepted 2008-AUG-24 by CDEROW. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1991 Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide. Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1991 Bischoff FR., Ponstingl H. Soluble fraction of HeLa was subject to ammonium sulphate precipitation followed by complexing with GDP; the complex so formed was purified on Sephacryl S-200. Further purification to homogenity was obtained on hydroxylapatite. in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay predetermined predetermined participant predetermined Bischoff FR., Ponstingl H. Soluble fraction of HeLa was subject to ammonium sulphate precipitation followed by complexing with GDP; the complex so formed was purified on Sephacryl S-200. Further purification to homogenity was obtained on hydroxylapatite. Accepted 2008-AUG-24 by CDEROW. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1991 Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide. Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1991 Bischoff FR., Ponstingl H. Soluble fraction of HeLa was subject to ammonium sulphate precipitation followed by complexing with GDP; the complex so formed was purified on Sephacryl S-200. Further purification to homogenity was obtained on hydroxylapatite. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Bischoff FR., Ponstingl H. Soluble fraction of HeLa was subject to ammonium sulphate precipitation followed by complexing with GDP; the complex so formed was purified on Sephacryl S-200. Further purification to homogenity was obtained on hydroxylapatite. Accepted 2008-AUG-24 by CDEROW. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1991 Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide. Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1991 Bischoff FR., Ponstingl H. Soluble fraction of HeLa was subject to ammonium sulphate precipitation followed by complexing with GDP; the complex so formed was purified on Sephacryl S-200. Further purification to homogenity was obtained on hydroxylapatite. in vitro In vitro chromatography chromatography technology column chromatography chromatography western blot western blot Immuno blot Immuno blot Bischoff FR., Ponstingl H. Soluble fraction of HeLa was subject to ammonium sulphate precipitation followed by complexing with GDP; the complex so formed was purified on Sephacryl S-200. Further purification to homogenity was obtained on hydroxylapatite. Accepted 2008-AUG-24 by CDEROW. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1991 gdp guanosine 5-diphosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ran_human GTP-binding nuclear protein Ran GTPase Ran Ras-related nuclear protein Ras-like protein TC4 Androgen receptor-associated protein 24 RAN ARA24 OK/SW-cl.81 protein protein human Homo sapiens Human MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL DIP protein P62826 original sequence version: 138 DIP protein P62826 original sequence version: 67 DIP protein P62826 original sequence version: 93 DIP protein P62826 original sequence version: 125 DIP protein P62826 original sequence version: 96 D5C9B7275C34BCE0 gtp guanosine triphosphate - GTP 5'-GTP GTP Guanosine 5'-triphosphate guanosine 5'-triphosphoric acid guanosine triphosphate GUANOSINE-5'-TRIPHOSPHATE H4gtp guanosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 rcc1_human Regulator of chromosome condensation Chromosome condensation protein 1 Cell cycle regulatory protein RCC1 CHC1 protein protein human Homo sapiens Human MSPKRIAKRRSPPADAIPKSKKVKVSHRSHSTEPGLVLTLGQGDVGQLGLGENVMERKKPALVSIPEDVVQAEAGGMHTVCLSKSGQVYSFGCNDEGALGRDTSVEGSEMVPGKVELQEKVVQVSAGDSHTAALTDDGRVFLWGSFRDNNGVIGLLEPMKKSMVPVQVQLDVPVVKVASGNDHLVMLTADGDLYTLGCGEQGQLGRVPELFANRGGRQGLERLLVPKCVMLKSRGSRGHVRFQDAFCGAYFTFAISHEGHVYGFGLSNYHQLGTPGTESCFIPQNLTSFKNSTKSWVGFSGGQHHTVCMDSEGKAYSLGRAEYGRLGLGEGAEEKSIPTLISRLPAVSSVACGASVGYAVTKDGRVFAWGMGTNYQLGTGQDEDAWSPVEMMGKQLENRVVLSVSSGGQHTVLLVKDKEQS DIP protein P18754 original sequence version: 117 F6D225AF81928305 ran-rcc1 2 9 unspecified role unspecified role neutral component neutral component human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 7 unspecified role unspecified role neutral component neutral component human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 direct interaction direct interaction 6B Two-dimensional gel electrophoresis of the 25-kDa Ran protein nucleoplasmic monomer (a) or complexed with 47-kDa RCC1 (b). purified RAN and RCC1 proteins used. ran-gdp 5 7 unspecified role unspecified role neutral component neutral component human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 6 unspecified role unspecified role neutral component neutral component direct interaction direct interaction 3 and 4 For purification, the protein was precipitated from the soluble fraction of HeLa lysate by ammonium sulfate, dissolved in 100 mM sodium phosphate (pH 7.0), incubated with GDP to obtain uniform nucleotide binding, and gel-filtered on Sephacryl S-200 (Fig. 3). Purification to homogeneity was achieved on hydroxylapatite (Fig. 4). ran-gdp-1 1 6 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 direct interaction direct interaction 5 Identity of the soluble monomeric form with the complexed 25-kDa protein from chromatin is indicated by immunological crossreaction, apparent molecular mass in gel electrophoresis, the elution pattern of CNBr fragments from a reversedphase column (Fig. 5), the amino acid sequences (see below), and the isoelectric points (Fig. 6). ran-gdp-2 4 7 unspecified role unspecified role neutral component neutral component human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 6 unspecified role unspecified role neutral component neutral component direct interaction direct interaction 3 and 4 For purification, the protein was precipitated from the soluble fraction of HeLa lysate by ammonium sulfate, dissolved in 100 mM sodium phosphate (pH 7.0), incubated with GDP to obtain uniform nucleotide binding, and gel-filtered on Sephacryl S-200 (Fig. 3). Purification to homogeneity was achieved on hydroxylapatite (Fig. 4). ran-gdp-3 3 6 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 direct interaction direct interaction 8 ran-gtp 3 8 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 direct interaction direct interaction 8 ran-rcc1-2 3 9 unspecified role unspecified role neutral component neutral component human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 7 unspecified role unspecified role neutral component neutral component human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 6 unspecified role unspecified role neutral component neutral component direct interaction direct interaction 8