IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts. Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts. Cell (0092-8674) 2008 imex curation Meng W., Mushika Y., Ichii T., Takeichi M. no Author believes interaction to be indirect so export stopped takeichi@cdb.riken.jp 09-April-2009: Contacted by JYOTI. jyoti Only protein-protein interactions 13 human-caco2 Human Caucasian colon adenocarcinoma caco2 Human Caucasian colon adenocarcinoma confocal microscopy confocal microscopy immunostaining immunostaining Meng W., Mushika Y., Ichii T., Takeichi M. no Tagged molecules were detected by direct fluorescence. Author believes interaction to be indirect so export stopped Data concerning N-cadherin was not curated as the origin of the clone could not be traced. takeichi@cdb.riken.jp Accepted 2009-MAR-23 by JYOTI. 09-April-2009: Contacted by JYOTI. Cell (0092-8674) 2008 jyoti Only protein-protein interactions 13 imex curation Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts. Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts. Cell (0092-8674) 2008 imex curation Meng W., Mushika Y., Ichii T., Takeichi M. no Author believes interaction to be indirect so export stopped takeichi@cdb.riken.jp 09-April-2009: Contacted by JYOTI. jyoti Only protein-protein interactions 13 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Meng W., Mushika Y., Ichii T., Takeichi M. no Author believes interaction to be indirect so export stopped takeichi@cdb.riken.jp Accepted 2009-MAR-23 by JYOTI. 09-April-2009: Contacted by JYOTI. Cell (0092-8674) 2008 jyoti Only protein-protein interactions 13 imex curation Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts. Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts. Cell (0092-8674) 2008 imex curation Meng W., Mushika Y., Ichii T., Takeichi M. no Author believes interaction to be indirect so export stopped takeichi@cdb.riken.jp 09-April-2009: Contacted by JYOTI. jyoti Only protein-protein interactions 13 human-caco2 Human Caucasian colon adenocarcinoma caco2 Human Caucasian colon adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Meng W., Mushika Y., Ichii T., Takeichi M. no Author believes interaction to be indirect so export stopped takeichi@cdb.riken.jp Accepted 2009-MAR-23 by JYOTI. 09-April-2009: Contacted by JYOTI. Cell (0092-8674) 2008 jyoti Only protein-protein interactions 13 imex curation Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts. Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts. Cell (0092-8674) 2008 imex curation Meng W., Mushika Y., Ichii T., Takeichi M. no Author believes interaction to be indirect so export stopped takeichi@cdb.riken.jp 09-April-2009: Contacted by JYOTI. jyoti Only protein-protein interactions 13 in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Meng W., Mushika Y., Ichii T., Takeichi M. no Author believes interaction to be indirect so export stopped takeichi@cdb.riken.jp Accepted 2009-MAR-23 by JYOTI. 09-April-2009: Contacted by JYOTI. Cell (0092-8674) 2008 jyoti Only protein-protein interactions 13 imex curation Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts. Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts. Cell (0092-8674) 2008 imex curation Meng W., Mushika Y., Ichii T., Takeichi M. no Author believes interaction to be indirect so export stopped takeichi@cdb.riken.jp 09-April-2009: Contacted by JYOTI. jyoti Only protein-protein interactions 13 human-caco2 Human Caucasian colon adenocarcinoma caco2 Human Caucasian colon adenocarcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Meng W., Mushika Y., Ichii T., Takeichi M. no Author believes interaction to be indirect so export stopped takeichi@cdb.riken.jp Accepted 2009-MAR-23 by JYOTI. 09-April-2009: Contacted by JYOTI. Cell (0092-8674) 2008 jyoti Only protein-protein interactions 13 imex curation Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts. Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts. Cell (0092-8674) 2008 imex curation Meng W., Mushika Y., Ichii T., Takeichi M. no Author believes interaction to be indirect so export stopped takeichi@cdb.riken.jp 09-April-2009: Contacted by JYOTI. jyoti Only protein-protein interactions 13 human-caco2 Human Caucasian colon adenocarcinoma caco2 Human Caucasian colon adenocarcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Meng W., Mushika Y., Ichii T., Takeichi M. no Author believes interaction to be indirect so export stopped takeichi@cdb.riken.jp Accepted 2009-MAR-23 by JYOTI. 09-April-2009: Contacted by JYOTI. Cell (0092-8674) 2008 jyoti Only protein-protein interactions 13 imex curation ctnd1_human Catenin delta-1 p120 catenin Cadherin-associated Src substrate p120(cas) CTNND1 KIAA0384 protein protein human Homo sapiens Human MDDSEVESTASILASVKEQEAQFEKLTRALEEERRHVSAQLERVRVSPQDANPLMANGTLTRRHQNGRFVGDADLERQKFSDLKLNGPQDHSHLLYSTIPRMQEPGQIVETYTEEDPEGAMSVVSVETSDDGTTRRTETTVKKVVKTVTTRTVQPVAMGPDGLPVDASSVSNNYIQTLGRDFRKNGNGGPGPYVGQAGTATLPRNFHYPPDGYSRHYEDGYPGGSDNYGSLSRVTRIEERYRPSMEGYRAPSRQDVYGPQPQVRVGGSSVDLHRFHPEPYGLEDDQRSMGYDDLDYGMMSDYGTARRTGTPSDPRRRLRSYEDMIGEEVPSDQYYWAPLAQHERGSLASLDSLRKGGPPPPNWRQPELPEVIAMLGFRLDAVKSNAAAYLQHLCYRNDKVKTDVRKLKGIPVLVGLLDHPKKEVHLGACGALKNISFGRDQDNKIAIKNCDGVPALVRLLRKARDMDLTEVITGTLWNLSSHDSIKMEIVDHALHALTDEVIIPHSGWEREPNEDCKPRHIEWESVLTNTAGCLRNVSSERSEARRKLRECDGLVDALIFIVQAEIGQKDSDSKLVENCVCLLRNLSYQVHREIPQAERYQEAAPNVANNTGPHAASCFGAKKGKDEWFSRGKKPIEDPANDTVDFPKRTSPARGYELLFQPEVVRIYISLLKESKTPAILEASAGAIQNLCAGRWTYGRYIRSALRQEKALSAIADLLTNEHERVVKAASGALRNLAVDARNKELIGKHAIPNLVKNLPGGQQNSSWNFSEDTVISILNTINEVIAENLEAAKKLRETQGIEKLVLINKSGNRSEKEVRAAALVLQTIWGYKELRKPLEKEGWKKSDFQVNLNNASRSQSSHSYDDSTLPLIDRNQKSDKKPDREEIQMSNMGSNTKSLDNNYSTPNERGDHNRTLDRSGDLGDMEPLKGTTPLMQDEGQESLEEELDVLVLDDEGGQVSYPSMQKI DIP protein O60716 original sequence version: 140 D5C37489A891F292 ctnd1_mouse Catenin delta-1 Kiaa0384 p120 catenin Cadherin-associated Src substrate p120(cas) Ctnnd1 Catns protein protein mouse Mus musculus Mouse MDDSEVESTASILASVKEQEAQFEKLTRALEEERRHVSAQLERVRVSPQDANSLMANGTLTRRHQNGRFVGDADLERQKFSDLKLNGPQDHNHLLYSTIPRMQEPGQIVETYTEEDPEGAMSVVSVETTDDGTTRRTETTVKKVVKTMTTRTVQPVPMGPDGLPVDASAVSNNYIQTLGRDFRKNGNGGPGPYVGQAGTATLPRNFHYPPDGYGRHYEDGYPGGSDNYGSLSRVTRIEERYRPSMEGYRAPSRQDVYGPQPQVRVGGSSVDLHRFHPEPYGLEDDQRSMGYDDLDYGMMSDYGTARRTGTPSDPRRRLRSYEDMIGEEVPPDQYYWAPLAQHERGSLASLDSLRKGMPPPSNWRQPELPEVIAMLGFRLDAVKSNAAAYLQHLCYRNDKVKTDVRKLKGIPILVGLLDHPKKEVHLGACGALKNISFGRDQDNKIAIKNCDGVPALVRLLRKARDMDLTEVITGTLWNLSSHDSIKMEIVDHALHALTDEVIIPHSGWEREPNEDCKPRHIEWESVLTNTAGCLRNVSSERSEARRKLRECDGLVDALIFIVQAEIGQKDSDSKLVENCVCLLRNLSYQVHREIPQAERYQEALPTVANSTGPHAASCFGAKKGKDEWFSRGKKPTEDPANDTVDFPKRTSPARGYELLFQPEVVRIYISLLKESKTPAILEASAGAIQNLCAGRWTYGRYIRSALRQEKALSAIAELLTSEHERVVKAASGALRNLAVDARNKELIGKHAIPNLVKNLPGGQQNSSWNFSEDTVVSILNTINEVIAENLEAAKKLRETQGIEKLVLINKSGNRSEKEVRAAALVLQTIWGYKELRKPLEKEGWKKSDFQVNLNNASRSQSSHSYDDSTLPLIDRNQKSDKKPDREEIPMSNMGSNTKSLDNNYSTLNERGDHNRTLDRSGDLGDMEPLKGAPLMQKI DIP protein P30999 original sequence version: 127 DIP protein P30999 original sequence version: 128 2F13DB7350355832 cadh1_human Cadherin-1 Epithelial cadherin Uvomorulin CAM 120/80 CDH1 CDHE UVO protein protein human Homo sapiens Human MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFEDCTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNTVGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQIPAILGILGGILALLILILLLLLFLRRRAVVKEPLLPPEDDTRDNVYYYDEEGGGEEDQDFDLSQLHRGLDARPEVTRNDVAPTLMSVPRYLPRPANPDEIGNFIDENLKAADTDPTAPPYDSLLVFDYEGSGSEAASLSSLNSSESDKDQDYDYLNEWGNRFKKLADMYGGGEDD DIP protein P12830 original sequence version: 191 DIP protein P12830 original sequence version: 185 DIP protein P12830 original sequence version: 108 DIP protein P12830 original sequence version: null DIP protein P12830 original sequence version: 134 DIP protein P12830 original sequence version: 147 DIP protein P12830 original sequence version: 174 DIP protein P12830 original sequence version: 189 DIP protein P12830 original sequence version: 156 DIP protein P12830 original sequence version: 201 DIP protein P12830 original sequence version: 117 E427118043A13C67 camp3_mouse Calmodulin-regulated spectrin-associated protein 3 Marshalin Camsap3 Protein Nezha Kiaa1543 protein protein mouse Mus musculus Mouse MVEAAPAGSGPLRRTFLVPEIKSLDQYDFSRAKAAASLAWVLRAAFGGAEHVPPELWEPFYTDQYAQEHVKPPVTRLLLSAELYCRAWRQALPQLEPSPSPSALLALLARRGTVPSLPEHPVREADLKHQPILMGAHLAVIDALMVAFSFEWTKTLPGPLALSSLEHKLLFWVDTTVRRLQEKTEQEAAQRASPAAPLDGASPAQPSIRYRKDRAIARRAPCFPNVTTLQDLASGAALAATIHCYCPQLLRLEEVCLKDPMSVADSLYNLQLVQDFCASHLPRGCPLSLEDLLYVPPPLKVNLVVLLAEMYMCFEVLKPDFVQAKDLPDGHAVSPRNTETVPSQNNSGSSSPVFNFRHPLLSPGGPQSPLRGSTGSLKSSPSMSHMEALGKAWNRQLSRPLSQAVSFSTPFGLDSDVDVVMGDPVLLRSVSSDSLGPPRPVSTSSRNSAQPAPESGDLPTIEEALQIIHSAEPRLLPDGAADGSFYLHSPEGLSKPPLSPYPPEGASKPLSDRLNKAPIYISHPENPSKSSPCSTGEILKPPPPSEGSPKAVASSPAANNSEVKMTSFAERKKQLVKAEAESGLGSPTSTPVAPEALSSEMSELGARLEEKRRAIEAQKRRIEAIFAKHRQRLGKSAFLQVQPREAAGEAEEEAELGSVPGGERPAGEGQGEPSLRHKSVTFSPDLGPVPPEGLGDYNRAVSKLSAALSSLQRDMQRLTDQQQRLLAPPEAPGPAPPPAAWVIPGPATGPKAASPSPARRAPAARRSPGPGPSPTPRSPKHARPAELKLAPLTRVLTPPHDVDSLPHLRKFSPSQVPVQTRSSILLSEGTPPEEPTTKPALIEIPLASLGEPAADEEGDGSPPGAEDSLEEEASSEGEPRSGLGFFYKDEDKPEDEMAQKRASLLERQQRRVEEARRRKQWQEAEKEQKREEAARLAQEAPGLAFTTPVVASAAPVATLAPTTRAMAPAEEEVGPRRGDFTRLEYERRAQLKLMDDLDKVLRPRASGTGGPGRGGRRATRPRSGCCDDSALARSPARGLLGSRLSKVYSQSTLSLSTVANEAPNNLGVKRPTSRAPSPSGLMSPSRLPGSRERDWENGSNASSPASVPEYTGPRLYKEPSAKSNKFIIHNALSHCCLAGKVNEPQKNRILEEIEKSKANHFLILFRDSSCQFRALYTLSGETEELSRLAGYGPRTVTPAMVEGIYKYNSDRKRFTQIPAKTMSMSVDAFTIQGHLWQSKKPTTPKKGGGTPK DIP protein Q80VC9 original sequence version: 92 1E14AA8640108CF4 pkha7_human Pleckstrin homology domain-containing family A member 7 PLEKHA7 protein protein human Homo sapiens Human MAAATVGRDTLPEHWSYGVCRDGRVFFINDQLRCTTWLHPRTGEPVNSGHMIRSDLPRGWEEGFTEEGASYFIDHNQQTTAFRHPVTGQFSPENSEFILQEEPNPHMSKQDRNQRPSSMVSETSTAGTASTLEAKPGPKIIKSSSKVHSFGKRDQAIRRNPNVPVVVRGWLHKQDSSGMRLWKRRWFVLADYCLFYYKDSREEAVLGSIPLPSYVISPVAPEDRISRKYSFKAVHTGMRALIYNSSTAGSQAEQSGMRTYYFSADTQEDMNAWVRAMNQAAQVLSRSSLKRDMEKVERQAVPQANHTESCHECGRVGPGHTRDCPHRGHDDIVNFERQEQEGEQYRSQRDPLEGKRDRSKARSPYSPAEEDALFMDLPTGPRGQQAQPQRAEKNGMLPASYGPGEQNGTGGYQRAFPPRTNPEKHSQRKSNLAQVEHWARAQKGDSRSLPLDQTLPRQGPGQSLSFPENYQTLPKSTRHPSGGSSPPPRNLPSDYKYAQDRASHLKMSSEERRAHRDGTVWQLYEWQQRQQFRHGSPTAPICLGSPEFTDQGRSRSMLEVPRSISVPPSPSDIPPPGPPRVFPPRRPHTPAERVTVKPPDQRRSVDISLGDSPRRARGHAVKNSSHVDRRSMPSMGYMTHTVSAPSLHGKSADDTYLQLKKDLEYLDLKMTGRDLLKDRSLKPVKIAESDTDVKLSIFCEQDRVLQDLEDKIRALKENKDQLESVLEVLHRQMEQYRDQPQHLEKIAYQQKLLQEDLVHIRAELSRESTEMENAWNEYLKLENDVEQLKQTLQEQHRRAFFFQEKSQIQKDLWRIEDVTAGLSANKENFRILVESVKNPERKTVPLFPHPPVPSLSTSESKPPPQPSPPTSPVRTPLEVRLFPQLQTYVPYRPHPPQLRKVTSPLQSPTKAKPKVEDEAPPRPPLPELYSPEDQPPAVPPLPREATIIRHTSVRGLKRQSDERKRDRELGQCVNGDSRVELRSYVSEPELATLSGDMAQPSLGLVGPESRYQTLPGRGLSGSTSRLQQSSTIAPYVTLRRGLNAESSKATFPRPKSALERLYSGDHQRGKMSAEEQLERMKRHQKALVRERKRTLGQGERTGLPSSRYLSRPLPGDLGSVC 93F616CF3EF9E5C2 camp3_human Calmodulin-regulated spectrin-associated protein 3 CAMSAP3 KIAA1543 Protein Nezha protein protein human Homo sapiens Human MVEAAPPGPGPLRRTFLVPEIKSLDQYDFSRAKAAASLAWVLRAAFGGAEHVPPELWEPFYTDQYAQEHVKPPVTRLLLSAELYCRAWRQALPQLETPPNPSALLALLARRGTVPALPERPVREADLRHQPILMGAHLAVIDALMAAFAFEWTKTLPGPLALTSLEHKLLFWVDTTVRRLQEKTEQEAAQRASPAAPADGAAPAQPSIRYRKDRVVARRAPCFPTVTSLQDLASGAALAATIHCYCPQLLRLEEVCLKDPMSVADSLYNLQLVQDFCASRLPRGCPLSLEDLLYVPPPLKVNLVVMLAELFMCFEVLKPDFVQVKDLPDGHAASPRGTEASPPQNNSGSSSPVFTFRHPLLSSGGPQSPLRGSTGSLKSSPSMSHMEALGKAWNRQLSRPLSQAVSFSTPFGLDSDVDVVMGDPVLLRSVSSDSLGPPRPAPARTPTQPPPEPGDLPTIEEALQIIHSAEPRLLPDGAADGSFYLHSPEGPSKPSLASPYLPEGTSKPLSDRPTKAPVYMPHPETPSKPSPCLVGEASKPPAPSEGSPKAVASSPAATNSEVKMTSFAERKKQLVKAEAEAGAGSPTSTPAPPEALSSEMSELSARLEEKRRAIEAQKRRIEAIFAKHRQRLGKSAFLQVQPREASGEAEAEAEEADSGPVPGGERPAGEGQGEPTSRPKAVTFSPDLGPVPHEGLGEYNRAVSKLSAALSSLQRDMQRLTDQQQRLLAPPEAPGSAPPPAAWVIPGPTTGPKAASPSPARRVPATRRSPGPGPSQSPRSPKHTRPAELRLAPLTRVLTPPHDVDSLPHLRKFSPSQVPVQTRSSILLAEETPPEEPAARPGLIEIPLGSLADPAAEDEGDGSPAGAEDSLEEEASSEGEPRVGLGFFYKDEDKPEDEMAQKRASLLERQQRRAEEARRRKQWQEVEKEQRREEAARLAQEEAPGPAPLVSAVPMATPAPAARAPAEEEVGPRKGDFTRQEYERRAQLKLMDDLDKVLRPRAAGSGGPGRGGRRATRPRSGCCDDSALARSPARGLLGSRLSKIYSQSTLSLSTVANEAHNNLGVKRPTSRAPSPSGLMSPSRLPGSRERDWENGSNASSPASVPEYTGPRLYKEPSAKSNKFIIHNALSHCCLAGKVNEPQKNRILEEIEKSKANHFLILFRDSSCQFRALYTLSGETEELSRLAGYGPRTVTPAMVEGIYKYNSDRKRFTQIPAKTMSMSVDAFTIQGHLWQGKKPTTPKKGGGTPK DIP protein Q9P1Y5 original sequence version: 89 5A597E13531443CE kifc3_human Kinesin-like protein KIFC3 KIFC3 protein protein human Homo sapiens Human MVPSRRTWNLGATPSLRGLWRVGRAPEPEPGMARPAPAPASPAARPFPHTGPGRLRTGRGKDTPVCGDEDSSARSAARPALAQCRALSVDWAGPGSPHGLYLTLQVEHLKEKLISQAQEVSRLRSELGGTDLEKHRDLLMVENERLRQEMRRCEAELQELRTKPAGPCPGCEHSQESAQLRDKLSQLQLEMAESKGMLSELNLEVQQKTDRLAEVELRLKDCLAEKAQEEERLSRRLRDSHETIASLRAQSPPVKYVIKTVEVESSKTKQALSESQARNQHLQEQVAMQRQVLKEMEQQLQSSHQLTARLRAQIAMYESELERAHGQMLEEMQSLEEDKNRAIEEAFARAQVEMKAVHENLAGVRTNLLTLQPALRTLTNDYNGLKRQVRGFPLLLQEALRSVKAEIGQAIEEVNSNNQELLRKYRRELQLRKKCHNELVRLKGNIRVIARVRPVTKEDGEGPEATNAVTFDADDDSIIHLLHKGKPVSFELDKVFSPQASQQDVFQEVQALVTSCIDGFNVCIFAYGQTGAGKTYTMEGTAENPGINQRALQLLFSEVQEKASDWEYTITVSAAEIYNEVLRDLLGKEPQEKLEIRLCPDGSGQLYVPGLTEFQVQSVDDINKVFEFGHTNRTTEFTNLNEHSSRSHALLIVTVRGVDCSTGLRTTGKLNLVDLAGSERVGKSGAEGSRLREAQHINKSLSALGDVIAALRSRQGHVPFRNSKLTYLLQDSLSGDSKTLMVVQVSPVEKNTSETLYSLKFAERVRSVELGPGLRRAELGSWSSQEHLEWEPACQTPQPSARAHSAPSSGTSSRPGSIRRKLQPSGKSRPLPV DIP protein Q9BVG8 original sequence version: 132 50983587270BDC2F ctnnd1-plekha7-1 4 11 unspecified role unspecified role prey prey human-caco2 Human Caucasian colon adenocarcinoma caco2 Human Caucasian colon adenocarcinoma 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association To search for proteins interacting with the N-terminal region of p120-catenin (p120), we generated a GST fusion protein for this domain (N-GST) or an N-terminus-deleted p120 (ΔN-GST; Figure 1A) and incubated them with lysates of human colon carcinoma Caco2 cells. ctnnd1-plekha7-2 2 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-caco2 Human Caucasian colon adenocarcinoma caco2 Human Caucasian colon adenocarcinoma physical association physical association The results of this pull-down assay showed that PLEKHA7-Flag was precipitated only with the N-GST (Figure 1C). Fig. 1C ctnnd1-plekha7-3 2 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human-caco2 Human Caucasian colon adenocarcinoma caco2 Human Caucasian colon adenocarcinoma 27 30 physical association physical association Further analysis determined that a portion from aa 538 to 696 of PLEKHA7 was required for its binding to p120 (Figure S1 available online). Supp. Fig. 1 ctnnd1-plekha7-4 6 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-caco2 Human Caucasian colon adenocarcinoma caco2 Human Caucasian colon adenocarcinoma physical association physical association PLEKHA7 associates with p120 and E-cadherin Fig. 1D cdh1-plekha7-2 1 11 unspecified role unspecified role neutral component neutral component human-caco2 Human Caucasian colon adenocarcinoma caco2 Human Caucasian colon adenocarcinoma 9 unspecified role unspecified role neutral component neutral component colocalization colocalization Double-immunostaining for endogenous PLEKHA7 and E-cadherin in Caco2 cells. Figs. 1E, 1G, Supp. Figs. 3A, 9C After depletion of PLEKHA7, the intensity of E-cadherin signals distributed along the ZA was strongly reduced; whereas the lower lateral components of E-cadherin were not much affected. Depletion of CTNND1 caused a simple proportional drop in the amount of PLEKHA7 at the ZA. 6 hours after treatment with nocodazole, PLEKHA7 becomes fragmented and E-cadherin signals colocalizing with PLEKHA7 are diminished. cdh1-plekha7-1 1 9 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-caco2 Human Caucasian colon adenocarcinoma caco2 Human Caucasian colon adenocarcinoma colocalization colocalization Intriguingly, the overexpressed PLEKHA7 molecules upregulated the level of E-cadherin accumulating at the ZA while concomitantly downregulating its level at the lower portions of the junction (Figure 1F). Fig. 1F PLEKHA7 upregulats the level of E-cadherin accumulating at the ZA while concomitantly downregulating its level at the lower portions of the junction. ctnnd1-plekha7-7 5 11 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-caco2 Human Caucasian colon adenocarcinoma caco2 Human Caucasian colon adenocarcinoma 10 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Caco2 cells were transiently transfected with PLEKHA7-Flag and Nezha-GFP or control GFP vector (left), and their lysates were subjected to immunoprecipitation with anti-GFP antibodies. Fig. 2B kiaa1543-plekha7-1 3 11 unspecified role unspecified role prey prey human-caco2 Human Caucasian colon adenocarcinoma caco2 Human Caucasian colon adenocarcinoma 12 unspecified role unspecified role bait bait physical association physical association Coimmunoprecipitation of endogenous Nezha and PLEKHA7 Fig. 2C kiaa1543-plekha7-2 1 11 unspecified role unspecified role neutral component neutral component human-caco2 Human Caucasian colon adenocarcinoma caco2 Human Caucasian colon adenocarcinoma 12 unspecified role unspecified role neutral component neutral component colocalization colocalization Endogenous Nezha and PLEKHA7 are colocalized at the ZA Fig. 2D KIAA01543 (Q9P1Y5) signals disappeared from the cell junctions, but not from the cytoplasm, on depletion of PLEKHA7. cdh1-kifc3 1 13 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component colocalization colocalization Caco2 cells were doubly immunostained for KIFC3 and E-cadherin. Figs. 5A When PLEKHA7 or Nezha is deleted, KIFC3 iss diminished at cell junctions but not at the centrosomes. ctnnd1-plekha7-8 1 7 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component human-caco2 Human Caucasian colon adenocarcinoma caco2 Human Caucasian colon adenocarcinoma colocalization colocalization Caco2 cells were transiently transfected with p120 siRNA, and then double-immunostained for p120 and PLEKHA7 Supp. Fig. 3A plekha7-kifc3-1 1 13 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Endogenous KIFC3 is increased at the sites where PLEKHA7 or Nezha is overexpressed. Supp. Fig. 8B When PLEKHA7 or Nezha is deleted, KIFC3 is diminished at cell junctions but not at the centrosomes. kiaa1543-kifc3-1 1 13 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Endogenous KIFC3 is increased at the sites where PLEKHA7 or Nezha is overexpressed. Supp. Fig. 8B When PLEKHA7 or Nezha is deleted, KIFC3 is diminished at cell junctions but not at the centrosomes.