IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface. Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface. Cell (0092-8674) 2008 imex curation Lin CH., Macgurn JA., Chu T., Stefan CJ., Emr SD. sde26@cornell.edu 09-April-2009: Contacted by JYOTI. davet Only protein-protein interactions 10 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Lin CH., Macgurn JA., Chu T., Stefan CJ., Emr SD. sde26@cornell.edu Accepted 2009-MAR-17 by DAVET. 09-April-2009: Contacted by JYOTI. Cell (0092-8674) 2008 davet Only protein-protein interactions 10 imex curation Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface. Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface. Cell (0092-8674) 2008 imex curation Lin CH., Macgurn JA., Chu T., Stefan CJ., Emr SD. sde26@cornell.edu 09-April-2009: Contacted by JYOTI. davet Only protein-protein interactions 10 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down anti tag western anti tag western blot anti tag western Lin CH., Macgurn JA., Chu T., Stefan CJ., Emr SD. sde26@cornell.edu Accepted 2009-MAR-17 by DAVET. 09-April-2009: Contacted by JYOTI. Cell (0092-8674) 2008 davet Only protein-protein interactions 10 imex curation Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface. Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface. Cell (0092-8674) 2008 imex curation Lin CH., Macgurn JA., Chu T., Stefan CJ., Emr SD. sde26@cornell.edu 09-April-2009: Contacted by JYOTI. davet Only protein-protein interactions 10 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Lin CH., Macgurn JA., Chu T., Stefan CJ., Emr SD. Yeast cells were stimulated with methionine, treated with crosslinkers (DSP and DTBP), and TCA precipitated. FLAG immunoprecipitates were analyzed by SDS PAGE and immunoblot. sde26@cornell.edu Accepted 2009-MAR-17 by DAVET. 09-April-2009: Contacted by JYOTI. Cell (0092-8674) 2008 davet Only protein-protein interactions 10 imex curation mup1_yeast High-affinity methionine permease MUP1 YGR055W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEGRTFLSQLNVFNKENYQFSSSTTKKEVSNSTVDADNGASDFEAGQQFATELDQGEKQLGILSCIGLICNRMLGTGVFAVSSTIYTLCGSVGLALIMWAVGAIIAISGLYVYMEFGTAIPKNGGEKNYLEAIFRKPKFFITCMYAAYIFFLGWAAGNSINTAIMFLTAADTEVTKWNQRGIGVAVVFFAFLINSLNVKIGLYLQNILGIFKIGIVLFISITGWVALGGGLKDGYQSHNFRNAFEGTETATAYGIVNALYSVIWSFVGYSNVNYALGEVKNPVRTLKIAGPTSMVFLAIIYIFVNIAYFAVVPKDKLISSKLILAADFFDIVFGGQAKRAAAALVGLSALGNVLSVIFSQGRIIQQLGREGVLPFSNFFASSKPFNSPMVGLFQHFIVCTVTILAPPPGDAYLLVQNLISYPMNIINFAISAGLLWIYWQRRQGKIEWNPPIKAGVFVTGFFTLSNLYLIIAPYVPPSNGESVYSSMPYWIHCVIAWGIFFFGGVYYVVWAQLLPRWGHYKLVSKDVLGEDGFWRVKIAKVYDDTIGDVDTQEDGVIETNIIEHYKSEQEKSL F95D342814710837 rod1_yeast Protein ROD1 ROD1 YOR018W OR26.08 Resistance to o-dinitrobenzene protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFSSSSRPSKEPLLFDIRLRNLDNDVLLIKGPPDEASSVLLSGTIVLSITEPIQIKSLALRLFGRLRLNIPTVLQTVHGPHKRYSKFERNIYSHFWDDFNIKSYFQNLYDNHNNGKITISSKSSTNLAALPKRKRALSTASLISSNGQTSASKNYHTLVKGNYEFPFSAIIPGSLVESVEGLPNAAVTYALEATIERPKQPDLICKKHLRVIRTLAIDAVELSETVSVDNSWPEKVDYTISIPTKAIAIGSSTMINILIVPILKGLKLGPVRISLVENSQYCGSYGGVINQERMVAKLKLKDPLKHVAQIKKKRSLNEAADEGVDTDTGEFQDKWEVRALLNIPASLTKCSQDCRILSNIKVRHKIKFTISLLNPDGHISELRAALPVQLFISPFVPVNVKTSDVIERTLKTFGPSYQVTSQHDNSFSSKNFVDDSEEDVIFQRSASALQLSSMPTIVSGSTLNINSTDAEATAVADTTMVTSLMVPPNYGNHVYDRVYGEVTNEDETSASASSSAVESQAIHNIQNLYISDSNNSNNPILAPNPQIKIEDDSLNNCDSRGDSVNNSNLNLVNSNLTISENWNNNSPSANRYNNIINAGLNSPSLTPSFAHLSRRNSYSRQTSSTSLKNDLELTDLSRVPSYDKAMKSDMIGEDLPPAYPEEELGVQENKKIELERPQILHHKSTSSLLPLPGSSKSSNNLKRSSSRTHLSHSPLPRNNSGSSVSLQQLARNNTDSSFNLNLSFTSAKSSTGSRHFPFNMTTSFTSNSSSKNNSHFDKTDSTSDANKPREEENYTSATHNRRSRSSSVRSNNSNSPLRQGTGSFANLMEMFTKRDRS FD9A64174B1DC443 FD9A64174B1DC443 art5_yeast Arrestin-related trafficking adapter 5 YGR068C ART5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFSLSSLSSSGGHSEQKERERISYFDIRINSPYKDIILIQGSPLELSSIPLSGNLVISVKNEIVVKKISLRLVGRFKLEFLQVGRYKKNSSSLASLVKEKRKIFECYWDNLLVSSKGDVLVGGENAENQHNSSSGRSTSNQDMDTSGNAIFLSKRSLSSPVFNKIIRRKTHSSHRKILELPENGVTGTPFEGLRENARSRSSSSNTLNNNSHSYSNRDGSGSSYLFLMKRGNYELPFNTMLPPEVCETIEGLQSGSILYSFEAIIDGRQLWDTDLSVHTSPHGPIGSTSTSGNGMRTKNKIIIKKFKYLRILRTLSMDNLAMQEEISVGNTWRDKLQYETSIPSRAVPIGSTTPVKIKIFPFEKNIRLDRIEMALIQYYAMKDSSAQIYDDEIAVMKITHLADFGPLTDKLDVDCPFTIPDNLKQITQDCCLQDNLIRVMHKLQVRILLQRQVDGEYKNLEIKAQLPMLLFISPHLPMKGRLVLFDKHDGKIHFRPGELVPLFLTTYPAQGLTPGVELNSTTTAHLALPQPPPNYHESTNDHLMPALQPLGADSVVLTVPSYEQAQAQASASSYVTGSVPAYCDDD C85B0D42116C041A rog3_yeast Protein ROG3 YFR022W ROG3 Revertant of glycogen synthase kinase mutation protein 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGFSSGKSTKKKPLLFDIRLKNVDNDVILLKGPPNEAPSVLLSGCIVLSINEPMQIKSISLRLYGKIQIDVPLERPQDASSSSLSSSPPKIRKYNKVFYNYAWDNVNLKEYLSGLRGQSGLAGSSSSSNILGTRQRAQSTSSLKSLKGSSSPSSCTLDKGNYDFPFSAILPGSLPESVESLPNCFVTYSMESVIERSKNYSDLICRKNIRVLRTISPAAVELSETVCVDNSWPDKVDYSISVPNKAVAIGSATPINISIVPLSKGLKLGSIKVVLFENYQYCDPFPPVISENRQVTELNLEDPLNESSGEFNGNGCFVNNPFFQPDHSFQDKWEIDTILQIPNSLSNCVQDCDVRSNIKVRHKLKFFIILINPDGHKSELRASLPIQLFISPFVALSIKPLSSSNLYSLFSTTNQKDENSSQEEEEEYLFSRSASVTGLELLADMRSGGSVPTISDLMTPPNYEMHVYDRLYSGSFTRTAVETSGTCTPLGSECSTVEDQQQDLEDLRIRLTKIRNQRDNLGLPPSASSAAASRSLSPLLNVPAPEDGTERILPQSALGPNSGSVPGVHSNVSPVLLSRSPAPSVSAHEVLPVPSGLNYPETQNLNKVPSYGKAMKYDIIGEDLPPSYPCAIQNVQPRKPSRVHSRNSSTTLSSSIPTSFHSSSFMSSTASPISIINGSRSSSSGVSLNTLNELTSKTSNNPSSNSMKRSPTRRRATSLAGFMGGFLSKGNKR 2BC6F7F24B9806A5 ldb19_yeast Protein LDB19 LDB19 Low dye-binding protein 19 O6152 YOR322C protein protein yeast Saccharomyces cerevisiae Baker's yeast MAFSRLTSTHQSNHNGYSNSNKKGQSLPLTLSIDVESPPCVLYGSAMESSGAVLSGLFTVTVVDPYSSAEDKSLKNTESNVSTTSKSLKRKSTFGSALSSRLSSLSASTSNISPSTSSTSISHSPTPANLRIMAGYTKITITSVTLSLVQKIHFHKPFVPNISSMQTCMNCKTKITNMKSWEIQSNTQDLSVGSHSYPFSYLIPGSVPCSSSLGATAETQVKYELIAVVTYIDPHRNSFSSGHSTPRKEGSSSKKRLLQLAMPIAVTRSIPRGPDKNSLRVFPPTELTAAAVLPNVVYPKSTFPLEMKLDGVSSGDRRWRMRKLSWRIEETTRVKAHACPVHKHELRQLEEQVKIKESEKSKKPRSHIKRYGELGPQIRVAVNSLENMPSQRLPGEPGREQAPNSSGPASTGNVGLDDENPVNEDEEDQPGSEFIHPSDDALRQELLMQQQRARQQQLQQELKNNSSLFTEEVRIISKGEMKSGWKTDFDNNGKIELVTEIDCMGLNSGVSNPVMHASTLQTPSTGNKKPSINVACDIQDPNLGLYVSHILAVEIVVAEETLQYANGQPIRKPNSKNKKETNNNTMNVHNPDQRLAELSPIFANRNTPKVRRMGPEDITPVNSNKSNHSTNKEKASNGASNSNIVSVPTGAARVLRMQFRLTVTERSGLGISWDEEVPPIYQDVELLSPPCYELSINNGIKNKLYSTMSTPVRSEDDFVGGSDEDIGNYESQGLEPGPNVQEVTITQNKLTIPPTAHHYQPASSSQRSLTTVQSPPLESVVSVQGSVPFRGHVLTPHSTRDIRIQNFSDFLDSNRITQ 3F3C10ABE1E44F99 rsp5_yeast E3 ubiquitin-protein ligase RSP5 RSP5 MDP1 NPI1 YER125W SYGP-ORF41 Reverses SPT-phenotype protein 5 HECT-type E3 ubiquitin transferase RSP5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPSSISVKLVAAESLYKRDVFRSPDPFAVLTIDGYQTKSTSAAKKTLNPYWNETFKFDDINENSILTIQVFDQKKFKKKDQGFLGVVNVRVGDVLGHLDEDTATSSGRPREETITRDLKKSNDGMAVSGRLIVVLSKLPSSSPHSQAPSGHTASSSTNTSSTTRTNGHSTSSTRNHSTSHPSRGTAQAVESTLQSGTTAATNTATTSHRSTNSTSSATRQYSSFEDQYGRLPPGWERRTDNFGRTYYVDHNTRTTTWKRPTLDQTEAERGNQLNANTELERRQHRGRTLPGGSSDNSSVTVQVGGGSNIPPVNGAAAAAFAATGGTTSGLGELPSGWEQRFTPEGRAYFVDHNTRTTTWVDPRRQQYIRTYGPTNTTIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQNVPQYKRDFRRKVIYFRSQPALRILPGQCHIKVRRKNIFEDAYQEIMRQTPEDLKKRLMIKFDGEEGLDYGGVSREFFFLLSHEMFNPFYCLFEYSAYDNYTIQINPNSGINPEHLNYFKFIGRVVGLGVFHRRFLDAFFVGALYKMMLRKKVVLQDMEGVDAEVYNSLNWMLENSIDGVLDLTFSADDERFGEVVTVDLKPDGRNIEVTDGNKKEYVELYTQWRIVDRVQEQFKAFMDGFNELIPEDLVTVFDERELELLIGGIAEIDIEDWKKHTDYRGYQESDEVIQWFWKCVSEWDNEQRARLLQFTTGTSRIPVNGFKDLQGSDGPRRFTIEKAGEVQQLPKSHTCFNRVDLPQYVDYDSMKQKLTLAVEETIGFGQE 6F1836384479E70F 6F1836384479E70F ecm21_yeast Protein ECM21 YBL101C YBL0814 ECM21 Extracellular mutant protein 21 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPFITSRPVAKNSSHSLSETDLNQSKGQPFQPSPTKKLGSMQQRRRSSTIRHALSSLLGGANVHSPAVLNNTTKGGNNNGNIRSSNTDAQLLGKKQNKQPPPNARRHSTTAIQGSISDSATTTPRSSTSDTNRRTSGRLSVDQEPRISGGRYSQIEEDSTVLDFDDDHNSSAVVSSDLSSTSLTRLANSKKFNEQFLIEYLTARGLLGPKTVLSNEYLKISISTSGESVFLPTISSNDDEYLSRLNGLNDGTDDAEADFFMDGIDQQEGNTPSLATTAAATESGGSINENRDTLLRENNSGDHPGSGSELNTRSVEIDSSMVSYSIAVIVSVKKPTRFTDMQLELCSRVKVFWNTGVPPTKTFNEEFYNAASMKWNLNDENFDLFVPLSISPDDQMIENNSNDRQMRLFKNIPTEERLYLDKTKTKASLLNAIDVNKTHLYQPGDYVFLVPVVFSNHIPETIYLPSARVSYRLRLATKAINRKGFYRQDSNSPQPIVSPDSSSSLSSTTSSLKLTETESAQAHRRISNTLFSKVKNHLHMSSHQLKNEESGEEDIFAEYPIKVIRTPPPVAVSTANKPIYINRVWTDSLSYEISFAQKYVSLNSEVPIKIKLAPICKNVCVKRIHVSITERVTFVSKGYEYEYDQTDPVAKDPYNPYYLDFASKRRKERSVSLFEIRTKEKGTRALREEIVENSFNDNLLSYSPFDDDSDSKGNPKERLGITEPIIIETKLKFPKYEDLDKRTAKIIPPYGIDAYTSIPNPEHAVANGPSHRRPSVIGFLSGHKGSKSHEENEKPVYDPKFHQTIIKSNSGLPVKTHTRLNTPKRGLYLDSLHFSNVYCRHKLEIMLRISKPDPECPSKLRHYEVLIDTPIFLVSEQCNSGNMELPTYDMATMEGKGNQVPLSMNSDFFGNTCPPPPTFEEAISVPASPIVSPMGSPNIMASYDPDLLSIQQLNLSRTTSVSGPSGYSDDAGVPNVNRNSISNANAMNGSISNSAFVSGNSGQGVARARATSVNDRSRFNNLDKLLSTPSPVNRSHNSSPTNGLSQANGTVRIPNATTENSKDKQNEFFKKGYTLANVKDDEEQEGIVSSSSADSLLSHGNEPPRYDEIVPLMSDEE 7DE365C1625B8BA9 csr2_yeast Transcription factor CSR2 CHS5 SPA2 rescue protein 2 CSR2 MRG19 YP9367.10 YPR030W protein protein yeast Saccharomyces cerevisiae Baker's yeast MQSTVPIAIASNGNKRDVVQNVSAGDEGDILQRLARNREMISTSLSPQKSSGFSGRRRSSSVRDALSSFFGTGNSPTSSMDDYSNLMNRNYSTASTAMCRGNSFPSDVGTKAYNITGSYQPDRHRNSVPYTTIDQLHTRQDTGLRRESDPVAAKQISSNNDIVRSFITHHASNSTMFINRVLSDYLADRGFIKQTPLYNKKSVLEISIATSAESVFLPTTKSDETEYLSLIHGSLNQARTQPVGSTNTAESDFLPSCPTMDTLNENNDLSLFPLHTQRTSPSNTARTGNAMDTSNSDRASPASNNNTTDADSFVASGNNNPMNNNNSPARNRHPNSHSRSLPNAWNSQMPSFSFALIFSLNKSTTLSDIKVELTSNVRVVWFNGLPPTKNVNEECYNIGSLDWTLNADNFNLFIPQGAKSPLDIVENHSNNRKLKVLQKLSMRKRRSFSNKAVLRENILNNLNASNSTNKLNAGVYVFTIPIVLASRIPESLYYPSARVSYSLRLATKLKDEHTQLVASRPRSSSISSPQKLRSYSCSDSYEYSQIDDTIEGETYNNDKNSTGKIAFPSSWLKSAKGRLKRNNSNGRSDNNGASSSGLAMQHDSEDTINLQYPLNLVRTPPEISVTTANKPLYINKVWENCLSYEISFAQKYVPLNGEIPITIKVAPLVKSLSVKRIRVSCREKISYRSKDYQYDFDQLDPLASDPCNPYHMRYLVRKKKDRSLPLFEVASKCTSGPSIREEVVTNTVDDNLLAYTSSKENNKDIPFSESFTVKTKLKFPKYCEVDATKAASLPPYGIDLFDPIKDPTQSENTSNNGNVLGFLVGRPNRASKTVHKIPQDKNHNEVNDTNGNSNTSLQTSSNVPIQHYTRLNKPRRGLYLDSMHFKNIQCSHKLEIVLRVSKTDSGSSKIIRHYEVIVDTPIYLISDLCNTSNIDLPTYDMATTESSKVLPPTFEEATSVSASPRSSVSYYPDDISMQQLNLSRSTSLANGYLSTLHPKTTAVSDSSNGAPIRDQQEQQARPLRTEDYALQMGNENNAYSNMDGLLSQDIFEQETAATLFKRDIVTMNFNNNIFTPRYSPRTFTNTDYNYNDNDNNDNDTEGPGPIIHPGPEPPRYDEISS 31F958BB8DB0B7E8 31F958BB8DB0B7E8 aly2_yeast Arrestin-related trafficking adapter 3 ART3 J0934 YJL084C ALY2 Arrestin-like protein 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPMDQSISSPLFPMEKDIDIPLDATPLAQSSSLQLFIHLAEPVVFLQGFDPQKTEYPSVVLRGCLVVRILKPTKLKSISLSFKGYSRTEWPEGIPPKRQEFVEIKDIVDHTWALYPPTEQKSKKKMDASAPNESNNAANNFLTKESGASLYRTLSDNETITSRKNSISGLSSLNLSPLGAPGNSSVNVKDRESRQRSRSSSVTSSNGPSRNLSPINLLKRATSPSVSHHNYKPTTTSIFSDLLNNTFTHNDAASHHGHHIPTSSNHLAMTSNNFTSGSGGEFFVFQPGDYIYAFEELIPQAYPESIKADFGFVEYFLFASIERPGAFKSNISARQVVNIVRTQAHNSVEESEPIIISRDWENQLYYDIVIASKDIILDAFLPITFKFAPLDKVTLHRIRIYVTETMEYYCREKKVHRMEPTKKFLLTEQKGPKLPNLPNDANLSKAKNMGNLLQDPKNGDLVNKEYEYQIFIPSRFNNHQQLHPDTSYENIKANHWIKICLRLSRVVDNKRKHYEISIDSPIHVLHRLCSHANTLLPSYDGHPASFPKETDSSISSILESSDDNINLYHNSNIFFPKEVLSSPVLSPNVQPLDILIPHLPSTSLTRNSRQFNRNSKSHPSDNTIFNSAKLKSNIYQPESLQRELASPQAIPLSPITSPMSNMEVPPPDFDFSSDFISDAASGTTTTEVSSSESSILPRDPPSYKDTVLHDNNQKRRPNSKHPTPPSLKASHPNKNSDKNSSETLNKKESMSKIEENKHKRETTPKKRENRDVKSLSTPQREESKDSTSTGNQSNEKNRKRVLSLSSSLHSSPNNSGFAHSALGNLSNESLRSLNRRESVQDNLPSTIRHDNPFFTDLNQVLIEDELKNHDKNELNRHSTNTSSTPASARSSFDYSGINISKDKLNMEPLLSKTETLTNKVNEDSFLRPNDSYVDLLEPSVDTTIDITAPYARNSSAWHPLQNDNDNNQFSPLLGSNENFLNAANAQNSAESDHNNDIFTQGSGLTESSKNSDSEERFISRLSSPEKVLINTLDNESGLQSINESTL EAC5711EE1AB1C66 aly1_yeast Arrestin-related trafficking adapter 6 ART6 YKR021W ALY1 Arrestin-like protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLQFNTENDTVAPVFPMEQDINAAPDAVPLVQTTTLQVFVKLAEPIVFLKGFETNGLSEIAPSILRGSLIVRVLKPNKLKSISITFKGISRTEWPEGIPPKREEFSDVETVVNHTWPFYQADDGMNSFTLEHHSSNNSSNRPSMSDEDYLLEKSGASVYIPPTAEPPKDNSNLSLDAYERNSLSSDNLSNKPVSSDVSHDDSKLLAIQKTPLPSSSRRGSVPANFHGNSLSPHTFISDLFTKTFSNSGATPSPEQEDNYLTPSKDSKEVFIFRPGDYIYTFEQPISQSYPESIKANFGSVEYKLSIDIERFGAFKSTIHTQLPIKVVRLPSDGSVEETEAIAISKDWKDLLHYDVVIFSKEIVLNAFLPIDFHFAPLDKVTLHRIRIYLTESMEYTCNSNGNHEKARRLEPTKKFLLAEHNGPKLPHIPAGSNPLKAKNRGNILLDEKSGDLVNKDFQFEVFVPSKFTNSIRLHPDTNYDKIKAHHWIKICLRLSKKYGDNRKHFEISIDSPIHILNQLCSHANTLLPSYESHFQYCDEDGNFAPAADQQNYASHHDSNIFFPKEVLSSPVLSPNVQKMNIRIPSDLPVVRNRAESVKKSKSDNTSKKNDQSSNVFASKQLVANIYKPNQIPRELTSPQALPLSPITSPILNYQPLSNSPPPDFDFDLAKRGAADSHAIPVDPPSYFDVLKADGIELPYYDTSSSKIPELKLNKSRETLASIEEDSFNGWSQIDDLSDEDDNDGDIASGFNFKLSTSAPSENVNSHTPILQSLNMSLDGRKKNRASLHATSVLPSTIRQNNQHFNDINQMLGSSDEDAFPKSQSLNFNKKLPILKINDNVIQSNSNSNNRVDNPEDTVDSSVDITAFYDPRMSSDSKFDWEVSKNHVDPAAYSVNVASENRVLDDFKKAFREKRK 44E43EB7683E4AC4 rsp5-ldb19-1 1 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined undefined mutation mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain Wed Jul 06 16:28:52 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'ppiy_ppcy-paia_paca' Q12502:p.[Pro679Ala;Tyr681Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 17:01:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro679ala,tyr681ala' Tue Jun 30 17:28:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro679Ala;Tyr681Ala]' Q12502:p.[Pro690Ala;Tyr692Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 20:03:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro690ala,tyr692ala' Tue Jun 30 17:45:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro690Ala;Tyr692Ala]' in vitro In vitro 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ww region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range ecoli Escherichia coli (strain K12) physical association physical association GST fused to the WW domains of Rsp5 (GST-3xWW) was purified and used to pull down in vitro-translated full-length Cvs7 containing either wild-type or mutated PY motifs. Fig. 2E Coordinates of WW domain of RSP5 not given in paper so inferred from UniProtKB entry mup1-swi4-1 3 8 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Yeast cells expressing Mup1-FLAG and/or Art1-HA were stimulated with methionine, treated with crosslinkers, and TCA precipitated. Fig. 4E rsp5-ldb19-2 2 8 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ww region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range ecoli Escherichia coli (strain K12) 30 34 physical association physical association Members of the ART Family of Proteins in Yeast Are Adaptors for Rsp5 Fig. 5B Coordinates of WW domain of RSP5 not given in paper so inferred from UniProtKB entry rsp5-ecm21 2 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ww region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association Members of the ART Family of Proteins in Yeast Are Adaptors for Rsp5 Fig. 5B Coordinates of WW domain of RSP5 not given in paper so inferred from UniProtKB entry A c-terminal fragment of ecm21 was required to bind but the coordinates were not given in the entry rsp5-yjl084c-1 2 12 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ww region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range ecoli Escherichia coli (strain K12) physical association physical association Members of the ART Family of Proteins in Yeast Are Adaptors for Rsp5 Fig. 5B Coordinates of WW domain of RSP5 not given in paper so inferred from UniProtKB entry rsp5-rod1-1 2 5 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ww region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range ecoli Escherichia coli (strain K12) physical association physical association Members of the ART Family of Proteins in Yeast Are Adaptors for Rsp5 Fig. 5B Coordinates of WW domain of RSP5 not given in paper so inferred from UniProtKB entry rsp5-ygr068c 2 6 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ww region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range ecoli Escherichia coli (strain K12) physical association physical association Members of the ART Family of Proteins in Yeast Are Adaptors for Rsp5 Fig. 5B Coordinates of WW domain of RSP5 not given in paper so inferred from UniProtKB entry rsp5-ygr068c-1 2 13 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ww region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range ecoli Escherichia coli (strain K12) physical association physical association Members of the ART Family of Proteins in Yeast Are Adaptors for Rsp5 Fig. 5B Coordinates of WW domain of RSP5 not given in paper so inferred from UniProtKB entry rsp5-rog3-1 2 9 unspecified role unspecified role bait bait ww region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association Members of the ART Family of Proteins in Yeast Are Adaptors for Rsp5 Fig. 5B Coordinates of WW domain of RSP5 not given in paper so inferred from UniProtKB entry rsp5-rog3-2 2 9 unspecified role unspecified role bait bait ww region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association Members of the ART Family of Proteins in Yeast Are Adaptors for Rsp5 Fig. 5B Coordinates of WW domain of RSP5 not given in paper so inferred from UniProtKB entry