IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Cardiolipin defines the interactome of the major ADP/ATP carrier protein of the mitochondrial inner membrane. Cardiolipin defines the interactome of the major ADP/ATP carrier protein of the mitochondrial inner membrane. J. Cell Biol. (0021-9525) 2008 Claypool SM., Oktay Y., Boontheung P., Loo JA., Koehler CM. Solubilized Mitochondria were purified on Ni affinity chromatography followed by immunoprecipitation with anti PC resin. AAC2 interacting proteins identified following 1-dimensional SDS-PAGE of CNAPed or BN-PAGE/SDS-PAGE of CNAPAAC2 Koehler@chem.ucla.edu 23-Feb-2010: Contacted by JYOTI. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down western blot western blot Immuno blot Immuno blot Claypool SM., Oktay Y., Boontheung P., Loo JA., Koehler CM. Solubilized Mitochondria were purified on Ni affinity chromatography followed by immunoprecipitation with anti PC resin. AAC2 interacting proteins identified following 1-dimensional SDS-PAGE of CNAPed or BN-PAGE/SDS-PAGE of CNAPAAC2 Koehler@chem.ucla.edu Accepted 2009-APR-13 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. J. Cell Biol. (0021-9525) 2008 orchard Cardiolipin defines the interactome of the major ADP/ATP carrier protein of the mitochondrial inner membrane. Cardiolipin defines the interactome of the major ADP/ATP carrier protein of the mitochondrial inner membrane. J. Cell Biol. (0021-9525) 2008 Claypool SM., Oktay Y., Boontheung P., Loo JA., Koehler CM. Solubilized Mitochondria were purified on Ni affinity chromatography followed by immunoprecipitation with anti PC resin. AAC2 interacting proteins identified following 1-dimensional SDS-PAGE of CNAPed or BN-PAGE/SDS-PAGE of CNAPAAC2 Koehler@chem.ucla.edu 23-Feb-2010: Contacted by JYOTI. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down ms participant Identification by mass spectrometry ms participant Claypool SM., Oktay Y., Boontheung P., Loo JA., Koehler CM. Solubilized Mitochondria were purified on Ni affinity chromatography followed by immunoprecipitation with anti PC resin. AAC2 interacting proteins identified following 1-dimensional SDS-PAGE of CNAPed or BN-PAGE/SDS-PAGE of CNAPAAC2 Koehler@chem.ucla.edu Accepted 2009-APR-13 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. J. Cell Biol. (0021-9525) 2008 orchard Cardiolipin defines the interactome of the major ADP/ATP carrier protein of the mitochondrial inner membrane. Cardiolipin defines the interactome of the major ADP/ATP carrier protein of the mitochondrial inner membrane. J. Cell Biol. (0021-9525) 2008 Claypool SM., Oktay Y., Boontheung P., Loo JA., Koehler CM. Solubilized Mitochondria were purified on Ni affinity chromatography followed by immunoprecipitation with anti PC resin. AAC2 interacting proteins identified following 1-dimensional SDS-PAGE of CNAPed or BN-PAGE/SDS-PAGE of CNAPAAC2 Koehler@chem.ucla.edu 23-Feb-2010: Contacted by JYOTI. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Claypool SM., Oktay Y., Boontheung P., Loo JA., Koehler CM. Solubilized Mitochondria were purified on Ni affinity chromatography followed by immunoprecipitation with anti PC resin. AAC2 interacting proteins identified following 1-dimensional SDS-PAGE of CNAPed or BN-PAGE/SDS-PAGE of CNAPAAC2 Koehler@chem.ucla.edu Accepted 2009-APR-13 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. J. Cell Biol. (0021-9525) 2008 orchard qcr1_yeast Cytochrome b-c1 complex subunit 1, mitochondrial COR1 QCR1 Ubiquinol-cytochrome c oxidoreductase core protein 1 Ubiquinol-cytochrome c reductase 44 kDa protein YBL0403 YBL045C Complex III subunit 1 Core protein I protein protein yeast Saccharomyces cerevisiae Baker's yeast MLRTVTSKTVSNQFKRSLATAVATPKAEVTQLSNGIVVATEHNPSAHTASVGVVFGSGAANENPYNNGVSNLWKNIFLSKENSAVAAKEGLALSSNISRDFQSYIVSSLPGSTDKSLDFLNQSFIQQKANLLSSSNFEATKKSVLKQVQDFEENDHPNRVLEHLHSTAFQNTPLSLPTRGTLESLENLVVADLESFANNHFLNSNAVVVGTGNIKHEDLVNSIESKNLSLQTGTKPVLKKKAAFLGSEVRLRDDTLPKAWISLAVEGEPVNSPNYFVAKLAAQIFGSYNAFEPASRLQGIKLLDNIQEYQLCDNFNHFSLSYKDSGLWGFSTATRNVTMIDDLIHFTLKQWNRLTISVTDTEVERAKSLLKLQLGQLYESGNPVNDANLLGAEVLIKGSKLSLGEAFKKIDAITVKDVKAWAGKRLWDQDIAIAGTGQIEGLLDYMRIRSDMSMMRW AE58739EFB695254 AE58739EFB695254 ggc1_yeast Mitochondrial GTP/GDP carrier protein 1 GGC1 SHM1 YDL198C D1214 YHM1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPHTDKKQSGLARLLGSASAGIMEIAVFHPVDTISKRLMSNHTKITSGQELNRVIFRDHFSEPLGKRLFTLFPGLGYAASYKVLQRVYKYGGQPFANEFLNKHYKKDFDNLFGEKTGKAMRSAAAGSLIGIGEIVLLPLDVLKIKRQTNPESFKGRGFIKILRDEGLFNLYRGWGWTAARNAPGSFALFGGNAFAKEYILGLKDYSQATWSQNFISSIVGACSSLIVSAPLDVIKTRIQNRNFDNPESGLRIVKNTLKNEGVTAFFKGLTPKLLTTGPKLVFSFALAQSLIPRFDNLLSK 3B0AB95EF563E85C cox6_yeast Cytochrome c oxidase subunit 6, mitochondrial COX6 YHR051W Cytochrome c oxidase polypeptide VI protein protein yeast Saccharomyces cerevisiae Baker's yeast MLSRAIFRNPVINRTLLRARPGAYHATRLTKNTFIQSRKYSDAHDEETFEEFTARYEKEFDEAYDLFEVQRVLNNCFSYDLVPAPAVIEKALRAARRVNDLPTAIRVFEALKYKVENEDQYKAYLDELKDVRQELGVPLKEELFPSSS 91F2AF286BD2FB1A 91F2AF286BD2FB1A cox5a_yeast Cytochrome c oxidase subunit 5A, mitochondrial COX5A YNL052W N2474 YNL2474W Cytochrome c oxidase polypeptide Va protein protein yeast Saccharomyces cerevisiae Baker's yeast MLRNTFTRAGGLSRITSVRFAQTHALSNAAVMDLQSRWENMPSTEQQDIVSKLSERQKLPWAQLTEPEKQAVWYISYGEWGPRRPVLNKGDSSFIAKGVAAGLLFSVGLFAVVRMAGGQDAKTMNKEWQLKSDEYLKSKNANPWGGYSQVQSK 1B1F8A3FE12CF783 cy1_yeast Cytochrome c1, heme protein, mitochondrial CYT1 Cytochrome b-c1 complex subunit 4 Complex III subunit 4 Complex III subunit IV CTC1 Ubiquinol-cytochrome c oxidoreductase cytochrome c1 subunit YOR29-16 YOR065W protein protein yeast Saccharomyces cerevisiae Baker's yeast MFSNLSKRWAQRTLSKSFYSTATGAASKSGKLTQKLVTAGVAAAGITASTLLYADSLTAEAMTAAEHGLHAPAYAWSHNGPFETFDHASIRRGYQVYREVCAACHSLDRVAWRTLVGVSHTNEEVRNMAEEFEYDDEPDEQGNPKKRPGKLSDYIPGPYPNEQAARAANQGALPPDLSLIVKARHGGCDYIFSLLTGYPDEPPAGVALPPGSNYNPYFPGGSIAMARVLFDDMVEYEDGTPATTSQMAKDVTTFLNWCAEPEHDERKRLGLKTVIILSSLYLLSIWVKKFKWAGIKTRKFVFNPPKPRK 6CB7A0813AA06AF5 adh3_yeast Alcohol dehydrogenase 3, mitochondrial ADH3 YMR083W YM9582.08 YADH-3 Alcohol dehydrogenase III protein protein yeast Saccharomyces cerevisiae Baker's yeast MLRTSTLFTRRVQPSLFSRNILRLQSTAAIPKTQKGVIFYENKGKLHYKDIPVPEPKPNEILINVKYSGVCHTDLHAWHGDWPLPVKLPLVGGHEGAGVVVKLGSNVKGWKVGDLAGIKWLNGSCMTCEFCESGHESNCPDADLSGYTHDGSFQQFATADAIQAAKIQQGTDLAEVAPILCAGVTVYKALKEADLKAGDWVAISGAAGGLGSLAVQYATAMGYRVLGIDAGEEKEKLFKKLGGEVFIDFTKTKNMVSDIQEATKGGPHGVINVSVSEAAISLSTEYVRPCGTVVLVGLPANAYVKSEVFSHVVKSINIKGSYVGNRADTREALDFFSRGLIKSPIKIVGLSELPKVYDLMEKGKILGRYVVDTSK AB849BC4E9D4EB8A AB849BC4E9D4EB8A cox9_yeast Cytochrome c oxidase subunit 9, mitochondrial COX9 YDL067C D2520 Cytochrome c oxidase polypeptide VIIA protein protein yeast Saccharomyces cerevisiae Baker's yeast MTIAPITGTIKRRVIMDIVLGFSLGGVMASYWWWGFHMDKINKREKFYAELAERKKQEN D228F428EA7DAC7C D228F428EA7DAC7C cox4_yeast Cytochrome c oxidase subunit 4, mitochondrial COX4 YGL187C G1362 Cytochrome c oxidase polypeptide IV protein protein yeast Saccharomyces cerevisiae Baker's yeast MLSLRQSIRFFKPATRTLCSSRYLLQQKPVVKTAQNLAEVNGPETLIGPGAKEGTVPTDLDQETGLARLELLGKLEGIDVFDTKPLDSSRKGTMKDPIIIESYDDYRYVGCTGSPAGSHTIMWLKPTVNEVARCWECGSVYKLNPVGVPNDDHHH AB2EE69878819E0F cyb_yeast Cytochrome b COB Ubiquinol-cytochrome c oxidoreductase complex cytochrome b subunit Q0105 Cytochrome b-c1 complex subunit CYTB Cytochrome b-c1 complex subunit 3 Complex III subunit 3 Complex III subunit III Complex III subunit CYTB CYTB protein protein yeast Saccharomyces cerevisiae Baker's yeast MAFRKSNVYLSLVNSYIIDSPQPSSINYWWNMGSLLGLCLVIQIVTGIFMAMHYSSNIELAFSSVEHIMRDVHNGYILRYLHANGASFFFMVMFMHMAKGLYYGSYRSPRVTLWNVGVIIFILTIATAFLGYCCVYGQMSHWGATVITNLFSAIPFVGNDIVSWLWGGFSVSNPTIQRFFALHYLVPFIIAAMVIMHLMALHIHGSSNPLGITGNLDRIPMHSYFIFKDLVTVFLFMLILALFVFYSPNTLGHPDNYIPGNPLVTPASIVPEWYLLPFYAILRSIPDKLLGVITMFAAILVLLVLPFTDRSVVRGNTFKVLSKFFFFIFVFNFVLLGQIGACHVEVPYVLMGQIATFIYFAYFLIIVPVISTIENVLFYIGRVNK 1B1B1616BBC2A798 qcr8_yeast Cytochrome b-c1 complex subunit 8, mitochondrial QCR8 Ubiquinol-cytochrome c reductase complex 11 kDa protein Complex III subunit VII Complex III subunit 8 Ubiquinol-cytochrome c oxidoreductase subunit 8 Ubiquinone-binding protein QP-C J0526 YJL166W protein protein yeast Saccharomyces cerevisiae Baker's yeast MGPPSGKTYMGWWGHMGGPKQKGITSYAVSPYAQKPLQGIFHNAVFNSFRRFKSQFLYVLIPAGIYWYWWKNGNEYNEFLYSKAGREELERVNV 9CB828E495F6ED9E 9CB828E495F6ED9E ucri_yeast Cytochrome b-c1 complex subunit Rieske, mitochondrial RIP1 Rieske iron-sulfur protein Complex III subunit 5 Ubiquinol-cytochrome c oxidoreductase iron-sulfur subunit YEL024W protein protein yeast Saccharomyces cerevisiae Baker's yeast MLGIRSSVKTCFKPMSLTSKRLISQSLLASKSTYRTPNFDDVLKENNDADKGRSYAYFMVGAMGLLSSAGAKSTVETFISSMTATADVLAMAKVEVNLAAIPLGKNVVVKWQGKPVFIRHRTPHEIQEANSVDMSALKDPQTDADRVKDPQWLIMLGICTHLGCVPIGEAGDFGGWFCPCHGSHYDISGRIRKGPAPLNLEIPAYEFDGDKVIVG 21981BD8492E86F3 21981BD8492E86F3 cyc1_yeast Cytochrome c isoform 1 CYC1 Cytochrome c aerobic isoform J1653 YJR048W protein protein yeast Saccharomyces cerevisiae Baker's yeast MTEFKAGSAKKGATLFKTRCLQCHTVEKGGPHKVGPNLHGIFGRHSGQAEGYSYTDANIKKNVLWDENNMSEYLTNPKKYIPGTKMAFGGLKKEKDRNDLITYLKKACE 1F8B6CB3B60C0BE8 1F8B6CB3B60C0BE8 qcr10_yeast Cytochrome b-c1 complex subunit 10, mitochondrial QCR10 Complex III subunit XI Complex III subunit 10 Ubiquinol-cytochrome c oxidoreductase subunit 10 Ubiquinol-cytochrome c reductase 8.5 kDa protein YHR001BW YHR001W-A protein protein yeast Saccharomyces cerevisiae Baker's yeast MAYTSHLSSKTGLHFGRLSLRSLTAYAPNLMLWGGASMLGLFVFTEGWPKFQDTLYKKIPLLGPTLEDHTPPEDKPN B9212B4FD194BB3E mpcp_yeast Mitochondrial phosphate carrier protein Phosphate transport protein J1837 YJR077C MIR1 mPic 1 Mitochondrial import receptor p32 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSVSAAPAIPQYSVSDYMKFALAGAIGCGSTHSSMVPIDVVKTRIQLEPTVYNKGMVGSFKQIIAGEGAGALLTGFGPTLLGYSIQGAFKFGGYEVFKKFFIDNLGYDTASRYKNSVYMGSAAMAEFLADIALCPLEATRIRLVSQPQFANGLVGGFSRILKEEGIGSFYSGFTPILFKQIPYNIAKFLVFERASEFYYGFAGPKEKLSSTSTTLLNLLSGLTAGLAAAIVSQPADTLLSKVNKTKKAPGQSTVGLLAQLAKQLGFFGSFAGLPTRLVMVGTLTSLQFGIYGSLKSTLGCPPTIEIGGGGH 89D1D97C49A9E8F1 89D1D97C49A9E8F1 pic2_yeast Mitochondrial phosphate carrier protein 2 Phosphate transport protein 2 Pi carrier isoform 2 YER053C PIC2 mPic 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MESNKQPRKIQLYTKEFYATCTLGGIIACGPTHSSITPLDLVKCRLQVNPKLYTSNLQGFRKIIANEGWKKVYTGFGATFVGYSLQGAGKYGGYEYFKHLYSSWLSPGVTVYLMASATAEFLADIMLCPFEAIKVKQQTTMPPFCNNVVDGWKKMYAESGGMKAFYKGIVPLWCRQIPYTMCKFTSFEKIVQKIYSVLPKKKEEMNALQQISVSFVGGYLAGILCAAVSHPADVMVSKINSERKANESMSVASKRIYQKIGFTGLWNGLMVRIVMIGTLTSFQWLIYDSFKAYVGLPTTG D8A54864F06F8264 ndh1_yeast External NADH-ubiquinone oxidoreductase 1, mitochondrial YMR145C YM9375.14C NDE1 NDH1 External NADH dehydrogenase 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MIRQSLMKTVWANSSRFSLQSKSGLVKYAKNRSFHAARNLLEDKKVILQKVAPTTGVVAKQSFFKRTGKFTLKALLYSALAGTAYVSYSLYREANPSTQVPQSDTFPNGSKRKTLVILGSGWGSVSLLKNLDTTLYNVVVVSPRNYFLFTPLLPSTPVGTIELKSIVEPVRTIARRSHGEVHYYEAEAYDVDPENKTIKVKSSAKNNDYDLDLKYDYLVVGVGAQPNTFGTPGVYEYSSFLKEISDAQEIRLKIMSSIEKAASLSPKDPERARLLSFVVVGGGPTGVEFAAELRDYVDQDLRKWMPELSKEIKVTLVEALPNILNMFDKYLVDYAQDLFKEEKIDLRLKTMVKKVDATTITAKTGDGDIENIPYGVLVWATGNAPREVSKNLMTKLEEQDSRRGLLIDNKLQLLGAKGSIFAIGDCTFHPGLFPTAQVAHQEGEYLAQYFKKAYKIDQLNWKMTHAKDDSEVARLKNQIVKTQSQIEDFKYNHKGALAYIGSDKAIADLAVGEAKYRLAGSFTFLFWKSAYLAMCLSFRNRVLVAMDWAKVYFLGRDSSI 10B1795E12E29C34 10B1795E12E29C34 vdac1_yeast Mitochondrial outer membrane protein porin 1 POR1 OMP2 VDAC1 YNL055C N2441 YNL2441C Voltage-dependent anion-selective channel protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSPPVYSDISRNINDLLNKDFYHATPAAFDVQTTTANGIKFSLKAKQPVKDGPLSTNVEAKLNDKQTGLGLTQGWSNTNNLQTKLEFANLTPGLKNELITSLTPGVAKSAVLNTTFTQPFFTARGAFDLCLKSPTFVGDLTMAHEGIVGGAEFGYDISAGSISRYAMALSYFAKDYSLGATLNNEQITTVDFFQNVNAFLQVGAKATMNCKLPNSNVNIEFATRYLPDASSQVKAKVSDSGIVTLAYKQLLRPGVTLGVGSSFDALKLSEPVHKLGWSLSFDA C99D03711AF9B54D C99D03711AF9B54D fmp10_yeast Uncharacterized mitochondrial membrane protein FMP10 FMP10 YER182W protein protein yeast Saccharomyces cerevisiae Baker's yeast MFKRIAIAQIRTYTNGVVFKTASKPKRRWIPWTIFGGSFLGGWYLTQHMTFTDLLAYWRYDALPKNADEVVKYHADLNRRLNGLPIVKQLENAGFVQVIANEEENLLVSRALNTPGGVAIPPRVYYNPSRRETVGLYHLGMKLTGYPFLIHGGILATVIEDLMKEAIRLEKGTKNINQETKNLSISYKFPTLANQFVVVRTTDLQQYGNKTKLKAELMDQSGNRTLVKANATFSSEQGNPKEEK A78338C86EA52766 A78338C86EA52766 dld1_yeast D-lactate dehydrogenase [cytochrome] 1, mitochondrial YDL174C DLD1 DLD D-lactate ferricytochrome C oxidoreductase protein protein yeast Saccharomyces cerevisiae Baker's yeast MLWKRTCTRLIKPIAQPRGRLVRRSCYRYASTGTGSTDSSSQWLKYSVIASSATLFGYLFAKNLYSRETKEDLIEKLEMVKKIDPVNSTLKLSSLDSPDYLHDPVKIDKVVEDLKQVLGNKPENYSDAKSDLDAHSDTYFNTHHPSPEQRPRIILFPHTTEEVSKILKICHDNNMPVVPFSGGTSLEGHFLPTRIGDTITVDLSKFMNNVVKFDKLDLDITVQAGLPWEDLNDYLSDHGLMFGCDPGPGAQIGGCIANSCSGTNAYRYGTMKENIINMTIVLPDGTIVKTKKRPRKSSAGYNLNGLFVGSEGTLGIVTEATVKCHVKPKAETVAVVSFDTIKDAAACASNLTQSGIHLNAMELLDENMMKLINASESTDRCDWVEKPTMFFKIGGRSPNIVNALVDEVKAVAQLNHCNSFQFAKDDDEKLELWEARKVALWSVLDADKSKDKSAKIWTTDVAVPVSQFDKVIHETKKDMQASKLINAIVGHAGDGNFHAFIVYRTPEEHETCSQLVDRMVKRALNAEGTCTGEHGVGIGKREYLLEELGEAPVDLMRKIKLAIDPKRIMNPDKIFKTDPNEPANDYR 07183BEAEEB2EB19 cox2_yeast Cytochrome c oxidase subunit 2 COX2 OXI1 Q0250 Cytochrome c oxidase polypeptide II protein protein yeast Saccharomyces cerevisiae Baker's yeast MLDLLRLQLTTFIMNDVPTPYACYFQDSATPNQEGILELHDNIMFYLLVILGLVSWMLYTIVMTYSKNPIAYKYIKHGQTIEVIWTIFPAVILLIIAFPSFILLYLCDEVISPAMTIKAIGYQWYWKYEYSDFINDSGETVEFESYVIPDELLEEGQLRLLDTDTSMVVPVDTHIRFVVTAADVIHDFAIPSLGIKVDATPGRLNQVSALIQREGVFYGACSELCGTGHANMPIKIEAVSLPKFLEWLNEQ BA5717C37330DF41 cox13_yeast Cytochrome c oxidase subunit 13, mitochondrial COX13 YGL191W G1341 Cytochrome c oxidase polypeptide VIa protein protein yeast Saccharomyces cerevisiae Baker's yeast MFRQCAKRYASSLPPNALKPAFGPPDKVAAQKFKESLMATEKHAKDTSNMWVKISVWVALPAIALTAVNTYFVEKEHAEHREHLKHVPDSEWPRDYEFMNIRSKPFFWGDGDKTLFWNPVVNRHIEHDD 91C3AD6EAC23CFD3 adt1_yeast ADP,ATP carrier protein 1 AAC1 Adenine nucleotide translocator 1 ADP/ATP translocase 1 YM9796.09C YMR056C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSHTETQTQQSHFGVDFLMGGVSAAIAKTGAAPIERVKLLMQNQEEMLKQGSLDTRYKGILDCFKRTATHEGIVSFWRGNTANVLRYFPTQALNFAFKDKIKSLLSYDRERDGYAKWFAGNLFSGGAAGGLSLLFVYSLDYARTRLAADARGSKSTSQRQFNGLLDVYKKTLKTDGLLGLYRGFVPSVLGIIVYRGLYFGLYDSFKPVLLTGALEGSFVASFLLGWVITMGASTASYPLDTVRRRMMMTSGQTIKYDGALDCLRKIVQKEGAYSLFKGCGANIFRGVAAAGVISLYDQLQLIMFGKKFK BEC8999DFBB1CA51 BEC8999DFBB1CA51 sco2_yeast Protein SCO2, mitochondrial YBR0308 YBR024W SCO2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLNSSRKYACRSLFRQANVSIKGLFYNGGAYRRGFSTGCCLRSDNKESPSARQPLDRLQLGDEINEPEPIRTRFFQFSRWKATIALLLLSGGTYAYLSRKRRLLETEKEADANRAYGSVALGGPFNLTDFNGKPFTEENLKGKFSILYFGFSHCPDICPEELDRLTYWISELDDKDHIKIQPLFISCDPARDTPDVLKEYLSDFHPAIIGLTGTYDQVKSVCKKYKVYFSTPRDVKPNQDYLVDHSIFFYLIDPEGQFIDALGRNYDEQSGLEKIREQIQAYVPKEERERRSKKWYSFIFN 247E1C4E91050E84 mss51_yeast Protein MSS51, mitochondrial MSS51 YLR203C L8167.17 Mitochondrial splicing suppressor protein 51 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTVLYAPSGATQLYFHLLRKSPHNRLVVSHQTRRHLMGFVRNALGLDPPPSPEDPTPENRFHPWDQSPSVDLRERAAKIRTLAHCPVTGKDINYTCPLSGIPTHHSREAWEMDKAYHDSKKYEILKKVNIYEHDLRSGRPFPEFDFPQQQGYDKAVNLTNWDLFFYTRSFYSMDTEFQLAAVTKMLSYPITIGSLLHKFSPYSLNPKGPITLEGLKSLAALRYTLYPLENRSLPTTTKNRAMRIFILGARAEAQLPGHVWKQLQFLFPEQSFEIHFIGPECLYKRDKQEYVKSTTPVVQRVDETLKFIYRTNFFEVFHEAQDFFPYDPYMDVFFTFHPGYASPESHGSWMGETMKALLETKCAIFTTGFNKKDLTDDINLVKSKYGKEMDVLMEPVRNVFGSTKWELNDMNPQEVYQFNMYIAGFRGKRYHTIKRQ 6A55BF160FE1D7DE dic1_yeast Mitochondrial dicarboxylate transporter DIC1 DTP YLR348C Dicarboxylate carrier 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTNAKESAGKNIKYPWWYGGAAGIFATMVTHPLDLAKVRLQAAPMPKPTLFRMLESILANEGVVGLYSGLSAAVLRQCTYTTVRFGAYDLLKENVIPREQLTNMAYLLPCSMFSGAIGGLAGNFADVVNIRMQNDSALEAAKRRNYKNAIDGVYKIYRYEGGLKTLFTGWKPNMVRGILMTASQVVTYDVFKNYLVTKLDFDASKNYTHLTASLLAGLVATTVCSPADVMKTRIMNGSGDHQPALKILADAVRKEGPSFMFRGWLPSFTRLGPFTMLIFFAIEQLKKHRVGMPKEDK C66A1F963450453D qcr7_yeast Cytochrome b-c1 complex subunit 7, mitochondrial QCR7 Ubiquinol-cytochrome c oxidoreductase subunit 7 Ubiquinol-cytochrome c reductase 14 kDa protein D9719.32 YDR529C Complex III subunit 7 Complex III subunit VII CRO1 UCR7 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPQSFTSIARIGDYILKSPVLSKLCVPVANQFINLAGYKKLGLKFDDLIAEENPIMQTALRRLPEDESYARAYRIIRAHQTELTHHLLPRNEWIKAQEDVPYLLPYILEAEAAAKEKDELDNIEVSK 1F1BA3DB6C4067B4 1F1BA3DB6C4067B4 fsf1_yeast Sideroflexin FSF1 FSF1 Fungal sideroflexin-1 YOR271C protein protein yeast Saccharomyces cerevisiae Baker's yeast MASSVPGPIDLPESRYDLSTYWGRIRHCAEISDPTMLLTTEKDLAHAREIISAYRHGELKETTPEFWRAKKQLDSTVHPDTGKTVLLPFRMSSNVLSNLVVTVGMLTPGLGTAGTVFWQWANQSLNVAVNSANANKSHPMSTSQLLTNYAAAVTASCGVALGLNNLVPRLKNISPHSKLILGRLVPFAAVVSAGIVNVFLMRGNEIRKGISVFDSNGDEVGKSKKAAFMAVGETALSRVINATPTMVIPPLILVRLQRGVLKGKSLGVQTLANLGLISVTMFSALPFALGIFPQRQAIHLNKLEPELHGKKDKDGKPIEKVYFNRGI FD175626E63B8619 qcr2_yeast Cytochrome b-c1 complex subunit 2, mitochondrial QCR2 Ubiquinol-cytochrome c oxidoreductase core protein 2 P9677.6 YPR191W Core protein II Complex III subunit 2 COR2 UCR2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLSAARLQFAQGSVRRLTVSARDAPTKISTLAVKVHGGSRYATKDGVAHLLNRFNFQNTNTRSALKLVRESELLGGTFKSTLDREYITLKATFLKDDLPYYVNALADVLYKTAFKPHELTESVLPAARYDYAVAEQCPVKSAEDQLYAITFRKGLGNPLLYDGVERVSLQDIKDFADKVYTKENLEVSGENVVEADLKRFVDESLLSTLPAGKSLVSKSEPKFFLGEENRVRFIGDSVAAIGIPVNKASLAQYEVLANYLTSALSELSGLISSAKLDKFTDGGLFTLFVRDQDSAVVSSNIKKIVADLKKGKDLSPAINYTKLKNAVQNESVSSPIELNFDAVKDFKLGKFNYVAVGDVSNLPYLDEL 51FDE1C5DA4420D0 51FDE1C5DA4420D0 sdhb_yeast Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial YLL041C SDH2 SDH SDHB Iron-sulfur subunit of complex II protein protein yeast Saccharomyces cerevisiae Baker's yeast MLNVLLRRKAFCLVTKKGMATATTAAATHTPRLKTFKVYRWNPDEPSAKPHLQSYQVDLNDCGPMVLDALLKIKDEQDSTLTFRRSCREGICGSCAMNIGGRNTLACICKIDQNESKQLKIYPLPHMFIVKDLVPDLTNFYQQYKSIQPYLQRSSFPKDGTEVLQSIEDRKKLDGLYECILCACCSTSCPSYWWNQEQYLGPAVLMQAYRWLIDSRDQATKTRKAMLNNSMSLYRCHTIMNCTRTCPKGLNPGLAIAEIKKSLAFA C14170F3670F6930 C14170F3670F6930 adt2_yeast ADP,ATP carrier protein 2 PET9 AAC2 YBL030C YBL0421 Adenine nucleotide translocator 2 Petite colonies protein 9 ADP/ATP translocase 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSNAQVKTPLPPAPAPKKESNFLIDFLMGGVSAAVAKTAASPIERVKLLIQNQDEMLKQGTLDRKYAGILDCFKRTATQEGVISFWRGNTANVIRYFPTQALNFAFKDKIKAMFGFKKEEGYAKWFAGNLASGGAAGALSLLFVYSLDYARTRLAADSKSSKKGGARQFNGLIDVYKKTLKSDGVAGLYRGFLPSVVGIVVYRGLYFGMYDSLKPLLLTGSLEGSFLASFLLGWVVTTGASTCSYPLDTVRRRMMMTSGQAVKYDGAFDCLRKIVAAEGVGSLFKGCGANILRGVAGAGVISMYDQLQMILFGKKFK A9805DC33D9E24AC A9805DC33D9E24AC hemh_yeast Ferrochelatase, mitochondrial YOR176W HEM15 Protoheme ferro-lyase Heme synthase protein protein yeast Saccharomyces cerevisiae Baker's yeast MLSRTIRTQGSFLRRSQLTITRSFSVTFNMQNAQKRSPTGIVLMNMGGPSKVEETYDFLYQLFADNDLIPISAKYQKTIAKYIAKFRTPKIEKQYREIGGGSPIRKWSEYQATEVCKILDKTCPETAPHKPYVAFRYAKPLTAETYKQMLKDGVKKAVAFSQYPHFSYSTTGSSINELWRQIKALDSERSISWSVIDRWPTNEGLIKAFSENITKKLQEFPQPVRDKVVLLFSAHSLPMDVVNTGDAYPAEVAATVYNIMQKLKFKNPYRLVWQSQVGPKPWLGAQTAEIAEFLGPKVDGLMFIPIAFTSDHIETLHEIDLGVIGESEYKDKFKRCESLNGNQTFIEGMADLVKSHLQSNQLYSNQLPLDFALGKSNDPVKDLSLVFGNHEST F04E7C7AAD36C24F F04E7C7AAD36C24F cox12_yeast Cytochrome c oxidase subunit 12, mitochondrial COX12 Cytochrome c oxidase polypeptide VIb YLR038C protein protein yeast Saccharomyces cerevisiae Baker's yeast MADQENSPLHTVGFDARFPQQNQTKHCWQSYVDYHKCVNMKGEDFAPCKVFWKTYNALCPLDWIEKWDDQREKGIFAGDINSD 57A8FAD93756577E aac2-mir1 2 15 unspecified role unspecified role prey prey 21 unspecified role unspecified role prey prey 29 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 25 unspecified role unspecified role prey prey 28 unspecified role unspecified role prey prey 34 unspecified role unspecified role prey prey 26 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 35 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 30 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 31 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 32 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 33 unspecified role unspecified role bait bait n terminal cnap Protein C epitope tag separated from a His10 tag by a 5 amino acid linker tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 19 unspecified role unspecified role prey prey association association table S1, 2B aac2-aac1 2 4 unspecified role unspecified role prey prey 33 unspecified role unspecified role bait bait n terminal cnap Protein C epitope tag separated from a His10 tag by a 5 amino acid linker tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 14 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 27 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 31 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 29 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 35 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 32 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 33 unspecified role unspecified role prey prey association association Table S2, 3B, 3E CNAP tagged AAC2 was transfected into cells where intrinsic AAC2 was deleted. aac2-cor1 2 31 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 29 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 35 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 33 unspecified role unspecified role bait bait n terminal cnap tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association Table S3, 3C, 3F CNAP tagged AAC2 was transfected into aac2 and crd1 deleted cells. The participant EBI-5024(P00410) was duplicated. It is posssible that originally this interaction did contain more participants pointing to different Trembl proteins which have been merged to a single swissprot protein later. aac2-cox2p 3 31 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 33 unspecified role unspecified role bait bait association association 4B Moreover, after IP of AAC2 from wt extracts, both complex III and IV subunits, but not complex V, Tim22p, Yta10p, or porin, were detected by immunoblot (Fig. 4 B). Anti-sera raised against n terminus of AAC2 used in the experiment. aac2-cor1-1 1 8 unspecified role unspecified role prey prey 31 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 33 unspecified role unspecified role bait bait n terminal cnap tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association The delta AAC2 and delta AAC2 CRD1 organisms show the same interactors. 2C aac2-rip1 3 14 unspecified role unspecified role prey prey 33 unspecified role unspecified role bait bait 23 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 31 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey association association 4C In contrast to wt extracts (all proteins expressed in same membranes), complex III and IV subunits failed to coIP with AAC2 when these proteins were expressed in separate membranes (Fig. 4 C Anti-sera raised against n terminus of AAC2 used in the experiment.