UniProt UniProt http://www.uniprot.org The RCC1 domain of protein associated with Myc (PAM) interacts with and regulates KCC2. The RCC1 domain of protein associated with Myc (PAM) interacts with and regulates KCC2. Cell. Physiol. Biochem. (1015-8987) 2008 imex curation Garbarini N., Delpire E. eric.delpire@vanderbilt.edu mouse brain cDNA orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Garbarini N., Delpire E. eric.delpire@vanderbilt.edu Accepted 2008-NOV-27 by ORCHARD. mouse brain cDNA Cell. Physiol. Biochem. (1015-8987) 2008 orchard Only protein-protein interactions imex curation The RCC1 domain of protein associated with Myc (PAM) interacts with and regulates KCC2. The RCC1 domain of protein associated with Myc (PAM) interacts with and regulates KCC2. Cell. Physiol. Biochem. (1015-8987) 2008 imex curation Garbarini N., Delpire E. eric.delpire@vanderbilt.edu mouse brain cDNA orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Garbarini N., Delpire E. eric.delpire@vanderbilt.edu Accepted 2008-NOV-27 by ORCHARD. mouse brain cDNA Cell. Physiol. Biochem. (1015-8987) 2008 orchard Only protein-protein interactions imex curation The RCC1 domain of protein associated with Myc (PAM) interacts with and regulates KCC2. The RCC1 domain of protein associated with Myc (PAM) interacts with and regulates KCC2. Cell. Physiol. Biochem. (1015-8987) 2008 imex curation Garbarini N., Delpire E. eric.delpire@vanderbilt.edu mouse brain cDNA orchard Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Garbarini N., Delpire E. eric.delpire@vanderbilt.edu Accepted 2008-NOV-27 by ORCHARD. mouse brain cDNA Cell. Physiol. Biochem. (1015-8987) 2008 orchard Only protein-protein interactions imex curation The RCC1 domain of protein associated with Myc (PAM) interacts with and regulates KCC2. The RCC1 domain of protein associated with Myc (PAM) interacts with and regulates KCC2. Cell. Physiol. Biochem. (1015-8987) 2008 imex curation Garbarini N., Delpire E. eric.delpire@vanderbilt.edu mouse brain cDNA orchard Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Garbarini N., Delpire E. eric.delpire@vanderbilt.edu Accepted 2008-NOV-27 by ORCHARD. mouse brain cDNA Cell. Physiol. Biochem. (1015-8987) 2008 orchard Only protein-protein interactions imex curation mycb2_mouse E3 ubiquitin-protein ligase MYCBP2 Mycbp2 Pam Phr1 Myc-binding protein 2 Protein associated with Myc Pam/highwire/rpm-1 protein Protein Magellan protein protein mouse Mus musculus Mouse MMMCAATASPAAASSGPGGDGFFAAATISSSPAPGALFMPVPDGSVAAAGLGLGLPTTDSRGHYQLLLSGRALADRYRRIYTTALSDRDQAGSSTGHPASRNKKILNKKKLKRKQKSKSKVKTRSKSENVENTVIIPDIKLHSNPSAFNIYCNVRHCVLEWQKKETSLAAASKNSVQSGESDSDEEEESREPPIKLPKIIEVGLCEVFELIKETRFSHPSLCLRSLQALLNVLQGQQPEGLQSEPPEVLESLFQLLLEITVRSTGMNDSTGQSLTALSCACLFSLVASWGETGRTLQAISAILTNNGSHACQTIQVPTILNSLQRSVQAVLVGKIQVQDWFSNGIKKAALMHKWPLKEVSVDEDDQCLLQNDGFFLYLLCKDGLYKIGSGYSGTVRGHIYNSTSRIRNRKEKKSWLGYAQGYLLYRDLNNHSMTAIRISPETLEQDGTVLLPDCHTEGQNILFTDGEYINQIAASRDDGFVVRIFATSTEPVLQQELQLKLARKCLHACGISLFDLEKDLHIISTGFDEESAILGAGREFALMKTANGKIYYTGKYQSLGIKQGGPSAGKWVELPITKSPKIVHFSVGHDGSHALLVAEDGSVFFTGSASKGEDGESTKSRRQSKPYKPKKIIKMEGKIVVYTACNNGSSSVISKDGELYMFGKDAIYSDSSSLVSDLKGHFVTQVAMGKAHTCVLMKNGEVWTFGVNNKGQCGRDTGAMNQGGKGFGVENMATAMDEDLEEELDEKDEKSMMCPPGMHKWKLEQCMVCTVCGDCTGYGASCVSSGRPDRVPGGICGCGSGESGCAVCGCCKACARELDGQEARQRGILDAVKEMIPLDLLLAVPVPGVNIEEHLQLRQEEKRQRVIRRHRLEDGRGPLVFAGPIFMNHREQALARLRSHPAQLKHKRDKHKDGSGDRGEKDASKITTYPPGSVRFDCELRAVQVSCGFHHSVVLMENGDVYTFGYGQHGQLGHGDVNSRGCPTLVQALPGPSTQVTAGSNHTAVLLMDGQVFTFGSFSKGQLGRPILDIPYWNAKPAPMPNIGSKYGRKATWIGASGDQTFLRIDEALINSHVLATSEIFASKHIIGLVPASISEPPPFKCLLINKVDGSCKTFNDSEQEDLQGFGVCLDPVYDVLWRFRPSTRELWCYNAVVADARLPSATDMQSRCSILSPELALPTGSRALTTRSHAALHILGCLDTLAAMQDLKMGIASTEEETQAVMKVYSKEDYSVVNRFESHGGGWGYSAHSVEAIRFSADTDILLGGLGLFGGRGEYTAKIKLFELGPDGGDHETDGDLLAETDVLAYDCAAREKYAMMFDEPVLLQAGWWYVAWARVSGPSSDCGSHGQASITTDDGVIFQFKSSKKSNNGTDVNAGQIPQLLYRLPTSDGSTSKGKQQTSEPVHILKRSFARTVSVECFESLLSILHWSWTTLVLGVEELRGLKGFQFTATLLDLERLRFVGTCCLRLLRVYTCEIYPVSATGKAVVEETSKLAECIGKTRTLLRKILSEGVDHCMVKLDNDPQGYLSQPLRLLEAVLQECHNTFTACFHSFYPTPALQWACLCDLLNCLDQEANFKTSSSRLLAAVMSALCHTSVKLTSLFPIAYDGEVLLRSIVKQVSTENDSTLVHRFPLLVGHMEKLSQSEENISGMTSFREVLEKMLVIVVLPVRNSLRRESELFSSHLVSNTCGLLASIVSELTASALGSEVDGLNSLHSVKASANRFTKTSQGRSWNTGNGSPDAICFAVDKPGIVVVGFAVYGGGGIHEYELEVLVDDSEHAGDSTHSHRWTSLELVKGTYTTDDSPSDIAEIRLDKVVPLKENVKYAVRLRNYGSRTANGDGGMTTVQCPDGVTFTFSTCSLSSNGTNQTRGQIPQILYYRSEFDGDLQSQLLSKANEEDKNCSRALSVVSTVVRAAKDLLHRALAVDADDIPELLSSSSLFSMLLPLIIAYIGPVAAAIPKVAVEVFGLVQQLLPSVAILNQKYAPPAFNPNQSTDSTTGNQPEQGLSACTTSNHYAVIESEHPYKPACVMHYKVTFPECVRWMTIEFDPQCGTAQSEDVIRLLIPVRTIQNSGYGAKLTSVHENLNSWVELKKYSGSSGWPTMVLVLPGNEALFSLETASDYVKDDKASFYGFKCFAIGYEFSPGPDEGVIQLEKELANLGGVCAAALMKKDLALPVGNELEEDLEILEEAALQVCKTHSGILGKGLALSHSPTILEALEGNLPLQIQSNEQSFLDDFIACVPGSSGGRLARWLQPDSYADPQKTSLILNKDDIRCGWPTTITVQTKDQYGDVVHVPNMKVEVKAVPVSQKKTSLQQDQGKKCQRIPGSPSAAASSADMTFGGLASPKLDVSYEPMIVKEARYIAITMMKVYENYSFEELRFASPTPKRPSENMLIRVNNDGTYCANWTPGAIGLYTVHVTIDGIEIDAGLEVKVKDPPKGMIPPGTQLVKPKADPQPNKIRKFVAKDSAGLRIRSHPSLQSEQIGIVRVNGTITFIDEIHNDDGVWLRLNEETIKKYVPNMNGYTEAWCLSFNQHLGKSLLVPVDNIFNASQGVRDLDVFSWTSKAFFPQEPKTNTDDFFKDMNSCGPQEATMQERDHPFLRGGPGMYKVVKTGPSGHNIRSCPNLRGIPIGMLVLGNKVKAVGEVTNSEGAWVQLDKNSMVEFCESDEGEAWSLARDRGGNQYLRHEDEQVLLDQNSQPPPPSPFSVQAFNKGASCSAQGFDYGLGNNKGDQLSAILNSIQSRPNLPAPSIFDQAAKPPSSLVHSPFVFGQPLSFQQRQLQSDRGTISTSSRPVSTSGKSELPSKHSRSVKPDGHVSRTPADQKKPRGTEGLSASESLMLKSDAAKLRSDSHSRSLSPNHNTLQTLKSDGRTSSGFRAESPGPGSRSSSPKPKPLPTPRSSPSGASSPRSSSPQDKNLPQKSTAPAKTKLDPPRERSKSDSYTLDPDTLRKKKMPLTEPLRGRSTSPKPKPVPKDPKDSPGSENRAPSPHVVQENLHSEVVEVCTSSTLKTNGVTDSTCDDSGDLKSVDEGSNKVHFSIGKAPLKDEQEMRASPKISRKCANRHTRPKKEKSNFLFKGDGTKSLEPAKQAMSPSVAECARAVFASFLWHEGIVHDAMACSSFLKFNPDLSKEHAPIRSSLNSQPPTEEKEIKLKNRHSLEISSALNMFNIAPHGPDISKMGSINKNKVLSMLKEPPLHEKCEDGKSEATFEMSMHHTMKSKSPLPLTLQHLVAFWEDISLATIKAASQNMIFPSPGSCAVLKKKECEKENKKTKKEKKKKEKTEIRPRGNLFGEMAQLAVGGPEKDTICELCGESHPYPVTYHMRQAHPGCGRYAGGQGYNSIGHFCGGWAGNCGDGGMGGSTWYLVCDRCREKYLREKQAAAREKVKQSRRKPMQVKTPRALPTMEAHQVIKANALFLLSLSSAAEPSILCYHPAKPFQSQLPIVKEGVSEDLPVKMPCLYLQTLARHHHENFVGYQDDNLFQDEMRYLRSTSVPAPYISVTPDASPNVFEEPESNMKSMPPSLETSPITDTDLAKRTVFQRSYSVVASEYDKQHSILPARVKAIPRRRVNSGDTVGSSLLRHPSPELSRLISAHSSLSKGERNFQWPVLAFVIQHHDLEGLEIAMKQALRKSACRVFAMEAFNWLLCNVIQTTSLHDILWHFVAALTPSPVEAEEDEDEDNKSNKENAEQEKDTRVCEHPLSDIVIAGEAAHPLPHTFHRLLQTISDLMMSLPSGSSLQQMALRCWSLKFKQSDHQFLHQSNVFHHINNILSKSDDGDSEESFSISVQSGFEAMSQELCIVMCLKDLTSIVDIKTSSRPAMIGSLTDGSTETFWESGDEDKNKTKNITINCVKGINARYVSVHVDNSRDLGNKVTSMTFLTGKAVEELCRIKQVDLDSRHIGWVTSELPGGDNQIIKIELKGPENTLRVRQVKVLGWKDGESTKIAGQISASVAQQRSCEAETLRVFRLITSQVFGKLISGDAEPTPEQEEKALLSSPEGEEKVYNATSDADLKEHMVGIIFSRSKLTNLQKQVCAHIVQAIRMEATRVREEWEHAISSKENANSQPSDEDASSDAYCFELLSMVLALSGSNVGRQYLAQQLTLLQDLFSLLHTASPRVQRQVTSLLRRVLPEVTPNRLASIIGVKSLPPADISDIIHSTEKGDWNKLGILDMFLGCIAKALTVQLKAKGTTITGTAGTTVGKGVTTVTLPMIFNSSYLRRGESHWWMKGSTPTQISEIIIRLIKDMAAGHLSEAWSRVTKNAIAETIIALTKMEEEFRSPVRCIATTRLWLALASLCVLDQDHVDRLSSGRWMGKDGQQKQMPMCDNHDDGETAAIILCNICGNLCTDCDRFLHLHRRTKTHQRQVFKEEEEAIKVDLHEGCGRTKLFWLMALADSKTMKAMVEFREHTGKPTTSSSEACRFCGSRSGTELSAVGSVCSDADCQEYAKIACSKTHPCGHPCGGVRNEEHCLPCLHGCDKSATTLKQDADDMCMICFTEALSAAPAIQLDCSHVFHLQCCRRVLENRWLGPRITFGFISCPICKNKINHIVLKDLLDPIKELYEDVRRKALMRLEYEGLHKSEAITTPGVRFYNDAAGYAMNRYAYYVCYKCRKAYFGGEARCDAEAGQGDDYDPRELICGACSDVSRAQMCPKHGTDFLEYKCRYCCSVAVFFCFGTTHFCNACHDDFQRMTSIPKEELPHCPAGPKGKQLEGTECPLHVVHPPTGEEFALGCGVCRNAHTF D225D8DCA55961E7 s12a5_rat Solute carrier family 12 member 5 Slc12a5 Kcc2 Electroneutral potassium-chloride cotransporter 2 K-Cl cotransporter 2 Neuronal K-Cl cotransporter Furosemide-sensitive K-Cl cotransporter protein protein rat Rattus norvegicus (Rat) Rat MSRRFTVTSLPPAASAASADPESRRHSVADPRRLPREDVKGDGNPKESSPFINSTDTEKGREYDGRNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSKEHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRLLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLTKGVIVERRGMPSVGLADGTPVDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLDNHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDESRGSIRRKNPANTRLRLNVPEETACDNEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGEGETDPEKVHLTWTKDKSAAQKNKGPSPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVNKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEQLDRVMLVRGGGREVITIYS 79748F3813EBEFA0 slc12a5-mycbp2 1 5 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association no figure mouse Mycbp2/PAM positions (paper: aa aa 551-874, total length 4641) can not be exactly mapped to SP as total length in SP is 4711 (or 4708 for isoform 2). But the overall location as RCC1 domain fits well. Q63633 remapped to parent entry as region of protein used in the 2 hybrid screen is not isoform specific. slc12a5-mycbp2-1 2 5 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q63633:p.Arg1104Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain R A Wed May 02 15:13:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1104ala' Tue Jun 30 20:05:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1104Ala' Q63633:p.Asn1105Glu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain N E Wed May 02 17:12:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn1105glu' Tue Jun 30 18:07:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn1105Glu' 16 14 physical association physical association rat KCC2 positions for c-term have been mapped to SP (isoform 1); isoform only diff at the n-ter hence interaction not presented as isoform-specific 5 and 6B mouse Mycbp2/PAM positions (paper: aa aa 551-874, total length 4641) can not be exactly mapped to SP as total length in SP is 4711 (or 4708 for isoform 2). But the overall location as RCC1 domain fits well. Carboxyterminal Slc12a5/KCC2 construct (aa 1092-1139) with the mutation tyr1110ala also interacts. slc12a5-mycbp2-2 3 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association rat KCC2 positions for c-term have been mapped to SP (isoform 1); isoform only diff at the n-ter hence interaction not presented as isoform-specific 1C mouse Mycbp2/PAM positions (paper: aa aa 551-874, total length 4641) can not be exactly mapped to SP as total length in SP is 4711 (or 4708 for isoform 2). But the overall location as RCC1 domain fits well. As seen in Figure 1, Panel C, only GST-KCC2-CT was able to pull-down RCC1/PAM (lane 2), whereas GST alone did not pull down RCC1/PAM. slc12a5-mycbp2-3 4 5 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 6 unspecified role unspecified role bait bait Q63633:p.Arg1104Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:38:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1104ala' Tue Jun 30 15:48:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1104Ala' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association 1D and 8