UniProt UniProt http://www.uniprot.org The endocytic recycling protein EHD2 interacts with myoferlin to regulate myoblast fusion. The endocytic recycling protein EHD2 interacts with myoferlin to regulate myoblast fusion. J. Biol. Chem. (0021-9258) 2008 imex curation Doherty KR., Demonbreun AR., Wallace GQ., Cave A., Posey AD., Heretis K., Pytel P., McNally EM. The polyclonal anti-myoferlin antibody, MYOF3, immunoprecipitated myoferlin as well as a approximat. 65 kDa band which was analyzed by MALDI-TOF mass spectrometry and contained EHD2. In further CoIP experiments with C2C12 myoblast cells an EHD2 antibody has been used for immunoblotting. emcnally@uchicago.edu 14-Nov-2008: Contacted by JYOTI. orchard Only protein-protein interactions mouse-c2c12 Mouse C3H muscle myoblast c2c12 Mouse C3H muscle myoblast anti bait coip anti bait coimmunoprecipitation anti bait coip fingerprinting peptide massfingerprinting fingerprinting Doherty KR., Demonbreun AR., Wallace GQ., Cave A., Posey AD., Heretis K., Pytel P., McNally EM. The polyclonal anti-myoferlin antibody, MYOF3, immunoprecipitated myoferlin as well as a approximat. 65 kDa band which was analyzed by MALDI-TOF mass spectrometry and contained EHD2. In further CoIP experiments with C2C12 myoblast cells an EHD2 antibody has been used for immunoblotting. emcnally@uchicago.edu Accepted 2008-NOV-16 by ORCHARD. 14-Nov-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2008 orchard Only protein-protein interactions imex curation The endocytic recycling protein EHD2 interacts with myoferlin to regulate myoblast fusion. The endocytic recycling protein EHD2 interacts with myoferlin to regulate myoblast fusion. J. Biol. Chem. (0021-9258) 2008 imex curation Doherty KR., Demonbreun AR., Wallace GQ., Cave A., Posey AD., Heretis K., Pytel P., McNally EM. The polyclonal anti-myoferlin antibody, MYOF3, immunoprecipitated myoferlin as well as a approximat. 65 kDa band which was analyzed by MALDI-TOF mass spectrometry and contained EHD2. In further CoIP experiments with C2C12 myoblast cells an EHD2 antibody has been used for immunoblotting. emcnally@uchicago.edu 14-Nov-2008: Contacted by JYOTI. orchard Only protein-protein interactions mouse-c2c12 Mouse C3H muscle myoblast c2c12 Mouse C3H muscle myoblast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Doherty KR., Demonbreun AR., Wallace GQ., Cave A., Posey AD., Heretis K., Pytel P., McNally EM. The polyclonal anti-myoferlin antibody, MYOF3, immunoprecipitated myoferlin as well as a approximat. 65 kDa band which was analyzed by MALDI-TOF mass spectrometry and contained EHD2. In further CoIP experiments with C2C12 myoblast cells an EHD2 antibody has been used for immunoblotting. emcnally@uchicago.edu Accepted 2008-NOV-16 by ORCHARD. J. Biol. Chem. (0021-9258) 2008 orchard Only protein-protein interactions imex curation The endocytic recycling protein EHD2 interacts with myoferlin to regulate myoblast fusion. The endocytic recycling protein EHD2 interacts with myoferlin to regulate myoblast fusion. J. Biol. Chem. (0021-9258) 2008 imex curation Doherty KR., Demonbreun AR., Wallace GQ., Cave A., Posey AD., Heretis K., Pytel P., McNally EM. The polyclonal anti-myoferlin antibody, MYOF3, immunoprecipitated myoferlin as well as a approximat. 65 kDa band which was analyzed by MALDI-TOF mass spectrometry and contained EHD2. In further CoIP experiments with C2C12 myoblast cells an EHD2 antibody has been used for immunoblotting. emcnally@uchicago.edu 14-Nov-2008: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Doherty KR., Demonbreun AR., Wallace GQ., Cave A., Posey AD., Heretis K., Pytel P., McNally EM. The polyclonal anti-myoferlin antibody, MYOF3, immunoprecipitated myoferlin as well as a approximat. 65 kDa band which was analyzed by MALDI-TOF mass spectrometry and contained EHD2. In further CoIP experiments with C2C12 myoblast cells an EHD2 antibody has been used for immunoblotting. emcnally@uchicago.edu Accepted 2008-NOV-16 by ORCHARD. 14-Nov-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2008 orchard Only protein-protein interactions imex curation myof_mouse Myoferlin Myof Kiaa1207 Fer1l3 Fer-1-like protein 3 protein protein mouse Mus musculus Mouse MLRVIVESATNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDSSSSLVIVVKDFETIGQNKLIGTATVSLKDLIGDQNRSLPYKQTSLLNEKGQDTGATIDLVIGYTPPSAPHPNDPSGTSVPGMGEEEEEDQGDEDRVDGIVRGPGPKGPSGTVSEAQLARRITKGKSSRRMLSNKPQDFQIRVRVIEGRQLCGNNIRPVVKVHICGQTHRTRIKRGNNPFFDELFFYNVHITPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGAKGYMKVSMFVLGTGDEPPPEKRDRDNDSDDVESNLLLPAGIALRWVTFMLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIERNANPEWNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEATTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNSGKGEGVAYRGRIFVELNTFLEKKPPEKKLEPISSDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLVMAERLQSNIEAVKSGIQGKIPANQLAEVWLKLIDEVIEDTRYTLPVTEGKANVTVLDTQIRKLRSRFLSQIHEAALRMRSEATDVKSTLLEIEEWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGGNASGKYCGKTQTILLKYPQEKTNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALVFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGDWVVDPERSLLTEADAGHTEFTDEVYQNENRYPGGEWKQAEDTYTDANGDKAASPSEMTCPPGWEWEDDAWIYDINRAVDEKGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELEDREGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGEEKGPEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIFGEPQTVLQNPPNVTIELFDNDQVGKDEFLGRSICSPLVKLNSETDITPKLLWHPVMNGDKACGDVLVTAELILRNKDGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLVVECGGERVESVVIKSLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIDHLDRFRCDPYAGKEDIVPQLKASLMSAPPCREVVIEIEDTKPLLASKLSEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYDCELEDVADFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVQGLQLQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETTIDLENRFLSRFGSHCGIPEQYCVSGVNTWRDQLRPTQLLQNVARFKGFPPPVLSEDGSRIRYGGRDYHLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEDMSDIYVKGWISGSEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRVPPRLIIQIWDNDKFSLDDYLGFLELDLHRTIIPAKTSEKCSLDMIPDLKAMDPLKAKTASLFEQRSMKGWWPCYADKDGTRVMAGKVEMTLEVLNEREADERPAGKGRSEPNMNPKLDPPNRPETSFLWFTNPCKTMRFIVWRRFKWVIIGLLLLLILLLFVAVLLYSLPNYLSMKIVRPNA 00D1596472CDFB8E myof_human Myoferlin MYOF KIAA1207 FER1L3 Fer-1-like protein 3 protein protein human Homo sapiens Human MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLGIIVKDFETIGQNKLIGTATVALKDLTGDQSRSLPYKLISLLNEKGQDTGATIDLVIGYDPPSAPHPNDLSGPSVPGMGGDGEEDEGDEDRLDNAVRGPGPKGPVGTVSEAQLARRLTKVKNSRRMLSNKPQDFQIRVRVIEGRQLSGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVNMTPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGSKGYMKVSMFVLGTGDEPPPERRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGTGAASYTVNTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNTGKGEGVAYRGRILVELATFLEKTPPDKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLAMAERLQTNIEALKSGIQGKIPANQLAELWLKLIDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRSLSQIHEAAVRMRSEATDVKSTLAEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGENASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGEWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYVYQARNLLALDKDSFSDPYAHICFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIYGEPQTVLQNPPKVIMELFDNDQVGKDEFLGRSIFSPVVKLNSEMDITPKLLWHPVMNGDKACGDVLVTAELILRGKDGSNLPILPPQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNFQMASITSPSLVVECGGERVESVVIKNLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIERLDRFRCDPYAGKEDIVPQLKASLLSAPPCRDIVIEMEDTKPLLASKLTEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYNCELENVAEFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEEYCVSGVNTWRDQLRPTQLLQNVARFKGFPQPILSEDGSRIRYGGRDYSLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRIPPRLIIQIWDNDKFSLDDYLGFLELDLRHTIIPAKSPEKCRLDMIPDLKAMNPLKAKTASLFEQKSMKGWWPCYAEKDGARVMAGKVEMTLEILNEKEADERPAGKGRDEPNMNPKLDLPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLFLLILLLFVAVLLYSLPNYLSMKIVKPNV 61D9E9447B40781C ehd2_mouse EH domain-containing protein 2 Ehd2 protein protein mouse Mus musculus Mouse MFSWLKKGGARGQRPEAIRTVTSSLKELYRTKLLPLEEHYRFGSFHSPALEDADFDGKPMVLVAGQYSTGKTSFIQYLLEQEVPGSRVGPEPTTDCFVAVMHGETEGTVPGNALVVDPEKPFRKLNPFGNTFLNRFMCAQLPNQVLESISIIDTPGILSGAKQRVSRGYDFPAVLRWFAERVDLIILLFDAHKLEISDEFSEAIGALRGHEDKIRVVLNKADMVETQQLMRVYGALMWALGKVVGTPEVLRVYIGSFWSQPLLVPDNRRLFELEEQDLFRDIQGLPRHAALRKLNDLVKRARLVRVHAYIISYLKKEMPTVFGKENKKKQLILKLPVIFAKIQLEHHISPGDFPDCQKMQELLMAHDFTKFHSLKPKLLEALDDMLAQDIAKLMPLLRQEELESVEAGVQGGAFEGTRMGPFVERGPDEAIEDGEEGSEDDAEWVVTKDKSKYDEIFYNLAPADGKLSGSKAKTWMVGTKLPNSVLGRIWKLSDVDRDGMLDDEEFALASHLIEAKLEGHGLPTNLPRRLVPPSKRRQKGSAE DIP protein Q8BH64 original sequence version: 101 DIP protein Q8BH64 original sequence version: 86 DIP protein Q8BH64 original sequence version: 109 47637795B0B1096E fer1l3-ehd2 2 4 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey association association 1D In addition, immunoblotting with an anti-EHD2 antibody confirmed that EHD2 was immunoprecipitated along with myoferlin in the presence of anti-myoferlin antibodies (Fig. 1D). fer1l3-ehd2-1 3 5 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain npf238_240spl binding region binding-associated region binding region mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q9NZM1:p.Asn238Cys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain N C Wed May 02 19:02:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn238cys' Tue Jun 30 19:23:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn238Cys' ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 2C and D a blot overlay was used to demonstrate a direct interaction between EHD2 and myoferlin. fer1l3-ehd2-2 1 4 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey association association 1B 14 prey proteins have been captured by MS/MS including eight keratin-related intermediate filament proteins and two RNA-binding proteins which have not been further specified. with the exception of EHD2, the author regarded these proteins as probable contaminants so they have not been included in the interaction.