MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Regulation of endocytic recycling by C. elegans Rab35 and its regulator RME-4, a coated-pit protein. Regulation of endocytic recycling by C. elegans Rab35 and its regulator RME-4, a coated-pit protein. EMBO J. (0261-4189) 2008 imex curation Sato M., Sato K., Liou W., Pant S., Harada A., Grant BD. sato-ken@showa.gunma-u.ac.jp 2009-01-08 Only protein-protein interactions caeel-oocyte Caenorhabditis elegans oocyte oocyte Oocyte fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Sato M., Sato K., Liou W., Pant S., Harada A., Grant BD. sato-ken@showa.gunma-u.ac.jp EMBO J. (0261-4189) 2008 2009-01-08 Only protein-protein interactions imex curation Regulation of endocytic recycling by C. elegans Rab35 and its regulator RME-4, a coated-pit protein. Regulation of endocytic recycling by C. elegans Rab35 and its regulator RME-4, a coated-pit protein. EMBO J. (0261-4189) 2008 imex curation Sato M., Sato K., Liou W., Pant S., Harada A., Grant BD. sato-ken@showa.gunma-u.ac.jp 2009-01-08 Only protein-protein interactions caeel Caenorhabditis elegans anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Sato M., Sato K., Liou W., Pant S., Harada A., Grant BD. sato-ken@showa.gunma-u.ac.jp EMBO J. (0261-4189) 2008 2009-01-08 Only protein-protein interactions imex curation Regulation of endocytic recycling by C. elegans Rab35 and its regulator RME-4, a coated-pit protein. Regulation of endocytic recycling by C. elegans Rab35 and its regulator RME-4, a coated-pit protein. EMBO J. (0261-4189) 2008 imex curation Sato M., Sato K., Liou W., Pant S., Harada A., Grant BD. sato-ken@showa.gunma-u.ac.jp 2009-01-08 Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Sato M., Sato K., Liou W., Pant S., Harada A., Grant BD. sato-ken@showa.gunma-u.ac.jp EMBO J. (0261-4189) 2008 2009-01-08 Only protein-protein interactions imex curation Regulation of endocytic recycling by C. elegans Rab35 and its regulator RME-4, a coated-pit protein. Regulation of endocytic recycling by C. elegans Rab35 and its regulator RME-4, a coated-pit protein. EMBO J. (0261-4189) 2008 imex curation Sato M., Sato K., Liou W., Pant S., Harada A., Grant BD. sato-ken@showa.gunma-u.ac.jp 2009-01-08 Only protein-protein interactions caeel Caenorhabditis elegans anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Sato M., Sato K., Liou W., Pant S., Harada A., Grant BD. sato-ken@showa.gunma-u.ac.jp EMBO J. (0261-4189) 2008 2009-01-08 Only protein-protein interactions imex curation g5ecw9_caeel rme-4 F46F6.1 CELE_F46F6.1 protein protein caeel Caenorhabditis elegans MTYGRYRADVATLYDVFCELDAPKPGEKLAILRLQYPDDFHDPAIVKSVQQFAFPCQLREVEVDAVQLFSFVLTDSNSKFSFGFCRYTPRTDTCICFLSGFFWPNVFFKALNDISLVIGSAQREDVESVLTKFYHTDIPNIDEYLRFADSNQTDGHNYRVVFAEKIPDHTRLPTLGSDKFFLEFYNAIDPRQMLAIFASLLKERRILFTGRKVGSLSSCLHAVSMLLYPMCWQSVFITILPESLVDMVMAPMPYLIGVPKTVLENARLNIRDIGEVVIVDIDEKTLTSPFDDVAAMPQEVVNFLKAQLRSQSAMDDTFAKHFLRAMVMLFGDYTSGFTGDTPETLVFSKERFVVQQKPSYQAYVSSLLGADGVQYLERFIHDRLELYKDGHVPGDQFEIEIEQMDLKNRPVKENANDVIGALKTSGIQIANRLQKLTPREIRKAAARKKAHNQNGFEQSPMSFDNLQWDATKSDENEGFPVETRMDNVPVANLIDLDEPSTSSASGLTSVDEQYFLRPSELATNNRKSPSGAIGNVFADPFATSPEGSAPPLPSRPPPPLHPRMIPGSSVQSISPSGSSAPHLLHFQQSHQWAQVSQVGSVPSAMNPSAPDWYAPPPLPPRRPASVGPSSTSSGQPATAFATSSAIRVPPPPVRSKWETFE 44658C7F444A66B3 clh_caeel Probable clathrin heavy chain 1 T20G5.1 chc-1 protein protein caeel Caenorhabditis elegans MALPIKFHEHLQLPNAGIRVPNITFSNVTMESDKNIVVREMIGDQQQVVIIDLADTANPTRRPISADSVIMHPTAKILALKSGKTLQIFNIELKAKVKAHQNVEDVVYWKWISEKTIALVSDTAVYHWSIEGDAAPVKMFDRHQSLAGTQIINYRADAENKWLVLIGISAKDSRVVGSMQLYSTERKVSQPIEGHAACFVRFKVDGNQNPSNLFCFSVKTDNGGKLHVIEVGTPAAGNTPFQKKNVDVPYTADTAGDFPVSMQVSAKQGIIYLVTKQGYVHLYDVESGTRIYSNRISTDTVFVTCEYTATGGIMGINRKGQVLSVSIDEANLVPFVTNQLQNPDLALKLAVRCDLPGAEELFVRKFNLLFSNGQFGESAKVAASAPQGILRTPATIQKFQQCPSTGPGPSPLLQYFGILLDQGKLNKYETLELCRPVLAQGRKELITKWLNDQKLECCEELGDLIKPHDVNTALSVYLRGNVPHKVVQSFAETGQFDKIVMYAKRVGFQPDYLFQLRQILRNSNPDHGAKFAQLLVSESENGEPLADLSQIIDCFMEVQAVQPCTSFLLEVLKGDKPEEGHLQTRLLEMNLLAAPAVADAILANKMFSHYDRAAIGQLCEKAGLLQRALEHFTDLYDIKRTVVHTHLLKPDWLVGYFGSLSVEDSVECLKAMLTQNIRQNLQVVVQIASKYHEQLGADKLIEMFENHKSYEGLFYFLGSIVNFSQDPEVHFKYIQAATRTGQIKEVERICRESQCYDAERVKNFLKEAKLNDQLPLIIVCDRHNMVHDLVLYLYRNQLQKYIEVFVQKVNAARLPIVVGALLDVDCSEDAIKQLIINTRGKFDIDELVEEVEKRNRLKLLNHWLESKIQEGATDAATHNAMAKIYIDSNNNPERFLKENPYYDSKVVGKYCEKRDPHYAFLSYERGQCDAELINVCNENSLFKNLARYLVKRRDFTLWEQVLNEENVHRRQLIDQVVQTALSETQDPEDISVTVKAFMAADLPNELIELLEKIVLDNSAFSEHRNLQNLLILTAMRADRTRVMEYIQKLDNYDAPDIANIAITSELYEEAFAIFKKFDVNSSAINVLIENVNNLDRAYEFAEKCNQSDVWASLAKAQLQQNLVKEAVDSFIKADDPGAYMEVVNKCSQTEHWEDLVRYLQMARKKSRESYIETELVFALAKTGRLTELEEFIAGPNHAQIGQIGDRCFDNGMFDSAKILFNNVSNFAKLSVTLVRLGEYQGAVDAARKANSTKTWKQVCFSCVENGEFRLAQMCGLHIVVHADELEELINFYQDRGHFEELIALLEAALGLERAHMGMFTELAILYSKYKPEKMREHLELFWSRVNIPKVLRAAEQAHLWSELVFLYDKYEEYDNAALTMMQHPTESWREQHFKEVIAKVANVELYYKAMQFYLDYKPLLLNDLLTVLSPRLDHSRTVLFFNKLKQIPLVKPYLRQVQNLNNKAINEALNQLLIDEEDHAGLRSSIEAQDNFDNITLAQQLEKHPLVEFRRISAYLFKGNNRWKQSIELCKKDKLYKDAMEYAAESRNGELAEELLSFFLDEKLYDCFAASLYHCYDLLHPDVIMELAWKHKIMDYAMPYMIQVMRDYQTRLEKLERSEHERKEEKAEQQQNNGMTMEPQLMLTYGAPAPQMTYPGTTGGYGGQPAYGQPGQPGYNAPGFM 44D15C61339009D9 q9u2c3_caeel CELE_Y47D3A.25 rab-35 Y47D3A.25 protein protein caeel Caenorhabditis elegans MAGTRDYDHLFKLLIIGDSGVGKSSLLLRFADNTFSENYITTIGVDFKIRTMDINGQRVKLQIWDTAGQERFRTITSTYYRGTHGVVVVYDVTNGESFGNVKRWLQEIENNCDSVQKVLVGNKCEENERRVVLESDARNYAQSMNISFFETSAKEDKNVEPMFTCITSLVLTAKLANPQSASKDQSRTGGVSLKDNSGSTNQKKKCKCG mint D53929429069264D q22601_caeel AP-2 complex subunit alpha T20B5.1 apa-2 CELE_T20B5.1 protein protein caeel Caenorhabditis elegans MPAVKGDGMRGLAVFISDIRNCKSKEAELKRINKELANIRSKFKGDKTLDGYQKKKYVCKLLFIFLLGNDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLIEQQSDLMKLIVQGIRNDLTSRNPVHVNLALQCISNMGSREMVEAFCTDLPKLLVSGETIDFVKQSAALCILKLFRNSPDSFQPGDYASRIVHLLNDSHMGVVTSAASLIEALSKKWPEEYKGAVPLAISRLSRIVTATYTDLQDYTYYFVPAPWLCVKLLRLLQNYPPPDDPSNKARLLECLEGILNKAQDAPKSKKVQHSNAKNAVLFEAIALIIHMDSEPQLLVRACNQLGTFLSHRETNLRYLALESMCLLATSEFSHDAVKKHQDTIINSLKTERDVSVRQRAVDLLYAMCDRSNANQIVAEMLAYLETADYSIREEMVLKVAILAEKYATDYTWYVDVILKLIRIAGDYVSEEVWYRVIQIVVNREDVQGYAAKTVFEALQRPACHENMVKVGGYILGEFGNFIAGDERSTAKIQFELLHSKFHLCSITTRCLLLTTYIKFCNLFPEIKPLVQQVFQTDHNLRNPDAELQQRSIEYLQMTKLASNDVLATILEVMPAFAEKESSLLAKLKKSKPQLEEIEREEKEKRSKPTAVMSEGSTSLVDFDSTNDTTASLADVFANNSGTGLGAQGDEVEIANKDDYLKFVTKSNAILWEDDYIQIGCKLETRNNLGRLGMFYGNKTSQPFNKFTPIITCPGALAVQLQAQAKPVEPVVAAGTQVQQLINFVCVQEFQKMPIMNIKFTFTDRAGAVQNFDKNFYLPLFISKFFEPTNMTSEQFFTRWKSLGAASQEAQKIFNALSPIEHATIESRLKGFGANLLTDVDPNPDNYVCAGIIHTQTQQIGTLIRLEPNKQAKMYRLTIRSSKDTVVQTLVDLLGNQF A816ADC042FD557C rab_35-rme_4 3 rab-35 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rme4 5 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f3e homomint mint apa_2-rme_4 3 rme4 5 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined apt4 8 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain range MINT-6604220: original sequence: 682-925 : ANKDDYLKFVTKSNAILWEDDYIQIGCKLETRNNLGRLGMFYGNKTSQPFNKFTPIITCPGALAVQLQAQAKPVEPVVAAGTQVQQLINFVCVQEFQKMPIMNIKFTFTDRAGAVQNFDKNFYLPLFISKFFEPTNMTSEQFFTRWKSLGAASQEAQKIFNALSPIEHATIESRLKGFGANLLTDVDPNPDNYVCAGIIHTQTQQIGTLIRLEPNKQAKMYRLTIRSSKDTVVQTLVDLLGNQ tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f7a mint rme_4-rab_35 4 rab-35 7 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell ha tagged ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9U2C3:p.Ser24Asn mutation increasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S N Tue Jul 12 10:07:27 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation increasing' Wed May 02 15:13:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser24asn' Tue Jun 30 18:40:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser24Asn' range MINT-6604145: original sequence: 24-24 : LIIGDSGVGKSSLLLRFADN rme4 5 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell gfp tagged gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f3f homomint mint rme_4-apa_2 4 apt4 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level rme4 5 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell gfp tagged gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f7c mint chc_1-rme_4 1 rme4 5 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tagged gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chc-1 6 immunostaining immunostaining predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization f6 homomint mint apa_2-rme_4-1 2 apt4 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level rme4 5 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell gfp tagged gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f7b mint