UniProt UniProt http://www.uniprot.org Casein kinase 1alpha interacts with RIP1 and regulates NF-kappaB activation. Casein kinase 1alpha interacts with RIP1 and regulates NF-kappaB activation. Biochemistry (0006-2960) 2008 imex curation Wang Y., Sun X., Wu J., Xu BE., Gu C., Wang H., Wang X., Tan F., Peng X., Qiang B., Yuan J., Luo Y. Apoptosis - Interactions involving proteins with a function related to apoptosis 293T cells have been cotransfected with the respective plasmids. yuanjiangang@pumc.edu.cn davet Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip antibody detection identification by antibody antibody detection Wang Y., Sun X., Wu J., Xu BE., Gu C., Wang H., Wang X., Tan F., Peng X., Qiang B., Yuan J., Luo Y. Apoptosis - Interactions involving proteins with a function related to apoptosis 293T cells have been cotransfected with the respective plasmids. yuanjiangang@pumc.edu.cn Accepted 2008-SEP-26 by DAVET. Biochemistry (0006-2960) 2008 davet Only protein-protein interactions imex curation Casein kinase 1alpha interacts with RIP1 and regulates NF-kappaB activation. Casein kinase 1alpha interacts with RIP1 and regulates NF-kappaB activation. Biochemistry (0006-2960) 2008 imex curation Wang Y., Sun X., Wu J., Xu BE., Gu C., Wang H., Wang X., Tan F., Peng X., Qiang B., Yuan J., Luo Y. Apoptosis - Interactions involving proteins with a function related to apoptosis 293T cells have been cotransfected with the respective plasmids. yuanjiangang@pumc.edu.cn davet Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Wang Y., Sun X., Wu J., Xu BE., Gu C., Wang H., Wang X., Tan F., Peng X., Qiang B., Yuan J., Luo Y. Apoptosis - Interactions involving proteins with a function related to apoptosis 293T cells have been cotransfected with the respective plasmids. yuanjiangang@pumc.edu.cn Accepted 2008-SEP-26 by DAVET. Biochemistry (0006-2960) 2008 davet Only protein-protein interactions imex curation Casein kinase 1alpha interacts with RIP1 and regulates NF-kappaB activation. Casein kinase 1alpha interacts with RIP1 and regulates NF-kappaB activation. Biochemistry (0006-2960) 2008 imex curation Wang Y., Sun X., Wu J., Xu BE., Gu C., Wang H., Wang X., Tan F., Peng X., Qiang B., Yuan J., Luo Y. Apoptosis - Interactions involving proteins with a function related to apoptosis 293T cells have been cotransfected with the respective plasmids. yuanjiangang@pumc.edu.cn davet Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wang Y., Sun X., Wu J., Xu BE., Gu C., Wang H., Wang X., Tan F., Peng X., Qiang B., Yuan J., Luo Y. Apoptosis - Interactions involving proteins with a function related to apoptosis 293T cells have been cotransfected with the respective plasmids. yuanjiangang@pumc.edu.cn Accepted 2008-SEP-26 by DAVET. Biochemistry (0006-2960) 2008 davet Only protein-protein interactions imex curation Casein kinase 1alpha interacts with RIP1 and regulates NF-kappaB activation. Casein kinase 1alpha interacts with RIP1 and regulates NF-kappaB activation. Biochemistry (0006-2960) 2008 imex curation Wang Y., Sun X., Wu J., Xu BE., Gu C., Wang H., Wang X., Tan F., Peng X., Qiang B., Yuan J., Luo Y. Apoptosis - Interactions involving proteins with a function related to apoptosis 293T cells have been cotransfected with the respective plasmids. yuanjiangang@pumc.edu.cn davet Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Wang Y., Sun X., Wu J., Xu BE., Gu C., Wang H., Wang X., Tan F., Peng X., Qiang B., Yuan J., Luo Y. Apoptosis - Interactions involving proteins with a function related to apoptosis 293T cells have been cotransfected with the respective plasmids. yuanjiangang@pumc.edu.cn Accepted 2008-SEP-26 by DAVET. Biochemistry (0006-2960) 2008 davet Only protein-protein interactions imex curation tnr1a_human Tumor necrosis factor receptor superfamily member 1A TNFRSF1A TNFAR TNFR1 p60 Tumor necrosis factor receptor 1 Tumor necrosis factor receptor type I p55 protein protein human Homo sapiens Human MGLSTVPDLLLPLVLLELLVGIYPSGVIGLVPHLGDREKRDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECTKLCLPQIENVKGTEDSGTTVLLPLVIFFGLCLLSLLFIGLMYRYQRWKSKLYSIVCGKSTPEKEGELEGTTTKPLAPNPSFSPTPGFTPTLGFSPVPSSTFTSSSTYTPGDCPNFAAPRREVAPPYQGADPILATALASDPIPNPLQKWEDSAHKPQSLDTDDPATLYAVVENVPPLRWKEFVRRLGLSDHEIDRLELQNGRCLREAQYSMLATWRRRTPRREATLELLGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR DIP protein P19438 original sequence version: 135 DIP protein P19438 original sequence version: 150 DIP protein P19438 original sequence version: 177 4CEFBA96D03B8225 ripk1_human Receptor-interacting serine/threonine-protein kinase 1 RIPK1 RIP Cell death protein RIP RIP1 Receptor-interacting protein 1 protein protein human Homo sapiens Human MQPDMSLNVIKMKSSDFLESAELDSGGFGKVSLCFHRTQGLMIMKTVYKGPNCIEHNEALLEEAKMMNRLRHSRVVKLLGVIIEEGKYSLVMEYMEKGNLMHVLKAEMSTPLSVKGRIILEIIEGMCYLHGKGVIHKDLKPENILVDNDFHIKIADLGLASFKMWSKLNNEEHNELREVDGTAKKNGGTLYYMAPEHLNDVNAKPTEKSDVYSFAVVLWAIFANKEPYENAICEQQLIMCIKSGNRPDVDDITEYCPREIISLMKLCWEANPEARPTFPGIEEKFRPFYLSQLEESVEEDVKSLKKEYSNENAVVKRMQSLQLDCVAVPSSRSNSATEQPGSLHSSQGLGMGPVEESWFAPSLEHPQEENEPSLQSKLQDEANYHLYGSRMDRQTKQQPRQNVAYNREEERRRRVSHDPFAQQRPYENFQNTEGKGTAYSSAASHGNAVHQPSGLTSQPQVLYQNNGLYSSHGFGTRPLDPGTAGPRVWYRPIPSHMPSLHNIPVPETNYLGNTPTMPFSSLPPTDESIKYTIYNSTGIQIGAYNYMEIGGTSSSLLDSTNTNFKEEPAAKYQAIFDNTTSLTDKHLDPIRENLGKHWKNCARKLGFTQSQIDEIDHDYERDGLKEKVYQMLQKWVMREGIKGATVGKLAQALHQCSRIDLLSSLIYVSQN DIP protein Q13546 original sequence version: 158 DIP protein Q13546-1 original sequence version: 140 DIP protein Q13546 original sequence version: 146 DIP protein Q13546-1 original sequence version: 146 DIP protein Q13546 original sequence version: 134 DIP protein Q13546 original sequence version: 109 976E2428D525A9B2 kc1a_human Casein kinase I isoform alpha CSNK1A1 CK1 protein protein human Homo sapiens Human MASSSGSKAEFIVGGKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQKARHPQLLYESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDLFNFCSRRFTMKTVLMLADQMISRIEYVHTKNFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTMLKQKAAQQAASSSGQGQQAQTPTGKQTDKTKSNMKGF DIP protein P48729 original sequence version: 92 DIP protein P48729 original sequence version: 171 DIP protein P48729 original sequence version: 163 1B2085AE33CAFAC2 tnfrsf1a-csnk1a1 3 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 5 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 2a ripk1-csnk1a1-1 3 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 6 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 2a and b, and 3b csnk1a1-ripk1 3 6 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 7 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 2c Treatment with TNFalpha did not change the interaction. csnk1a1-ripk1-1 1 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 3a Treatment with TNFalpha did not change the interaction. csnk1a1-ripk1-2 4 6 enzyme target enzyme target substrate substrate neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q13546:p.Lys45Ala mutation mutation certain certain sequence position certain certain certain sequence position certain K A Wed May 02 14:30:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys45ala' Tue Jun 30 20:21:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys45Ala' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 7 enzyme enzyme neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation 4a csnk1a1-ripk1-3 2 6 enzyme target enzyme target substrate substrate neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 enzyme enzyme neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation 4c