MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it SAGA and a novel Drosophila export complex anchor efficient transcription and mRNA export to NPC. SAGA and a novel Drosophila export complex anchor efficient transcription and mRNA export to NPC. EMBO J. (0261-4189) 2007 imex curation Kurshakova MM., Krasnov AN., Kopytova DV., Shidlovskii YV., Nikolenko JV., Nabirochkina EN., Spehner D., Schultz P., Tora L., Georgieva SG. laszlo@igbmc.u-strasbg.fr, sonjag@molbiol.edu.ru 23-Feb-2010: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kurshakova MM., Krasnov AN., Kopytova DV., Shidlovskii YV., Nikolenko JV., Nabirochkina EN., Spehner D., Schultz P., Tora L., Georgieva SG. laszlo@igbmc.u-strasbg.fr, sonjag@molbiol.edu.ru Accepted 2010-MAR-1 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. drosophila, library of 1x10^7 cDNA clones fused to GAL4-activating domain in pACT2 vector EMBO J. (0261-4189) 2007 orchard Only protein-protein interactions imex curation SAGA and a novel Drosophila export complex anchor efficient transcription and mRNA export to NPC. SAGA and a novel Drosophila export complex anchor efficient transcription and mRNA export to NPC. EMBO J. (0261-4189) 2007 imex curation Kurshakova MM., Krasnov AN., Kopytova DV., Shidlovskii YV., Nikolenko JV., Nabirochkina EN., Spehner D., Schultz P., Tora L., Georgieva SG. laszlo@igbmc.u-strasbg.fr, sonjag@molbiol.edu.ru 23-Feb-2010: Contacted by JYOTI. orchard Only protein-protein interactions drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. confocal microscopy confocal microscopy polyclonal immunost polyclonal antibody immunostaining polyclonal immunost Kurshakova MM., Krasnov AN., Kopytova DV., Shidlovskii YV., Nikolenko JV., Nabirochkina EN., Spehner D., Schultz P., Tora L., Georgieva SG. laszlo@igbmc.u-strasbg.fr, sonjag@molbiol.edu.ru Accepted 2010-MAR-1 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. EMBO J. (0261-4189) 2007 orchard Only protein-protein interactions imex curation SAGA and a novel Drosophila export complex anchor efficient transcription and mRNA export to NPC. SAGA and a novel Drosophila export complex anchor efficient transcription and mRNA export to NPC. EMBO J. (0261-4189) 2007 imex curation Kurshakova MM., Krasnov AN., Kopytova DV., Shidlovskii YV., Nikolenko JV., Nabirochkina EN., Spehner D., Schultz P., Tora L., Georgieva SG. laszlo@igbmc.u-strasbg.fr, sonjag@molbiol.edu.ru 23-Feb-2010: Contacted by JYOTI. orchard Only protein-protein interactions drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip polyclonal western polyclonal antibody western blot polyclonal western Kurshakova MM., Krasnov AN., Kopytova DV., Shidlovskii YV., Nikolenko JV., Nabirochkina EN., Spehner D., Schultz P., Tora L., Georgieva SG. laszlo@igbmc.u-strasbg.fr, sonjag@molbiol.edu.ru Accepted 2010-MAR-1 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. EMBO J. (0261-4189) 2007 orchard Only protein-protein interactions imex curation SAGA and a novel Drosophila export complex anchor efficient transcription and mRNA export to NPC. SAGA and a novel Drosophila export complex anchor efficient transcription and mRNA export to NPC. EMBO J. (0261-4189) 2007 imex curation Kurshakova MM., Krasnov AN., Kopytova DV., Shidlovskii YV., Nikolenko JV., Nabirochkina EN., Spehner D., Schultz P., Tora L., Georgieva SG. laszlo@igbmc.u-strasbg.fr, sonjag@molbiol.edu.ru 23-Feb-2010: Contacted by JYOTI. orchard Only protein-protein interactions drome-salivary_gland Drosophila melanogaster Salivary gland salivary_gland Salivary gland confocal microscopy confocal microscopy polyclonal immunost polyclonal antibody immunostaining polyclonal immunost Kurshakova MM., Krasnov AN., Kopytova DV., Shidlovskii YV., Nikolenko JV., Nabirochkina EN., Spehner D., Schultz P., Tora L., Georgieva SG. laszlo@igbmc.u-strasbg.fr, sonjag@molbiol.edu.ru Accepted 2010-MAR-1 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. EMBO J. (0261-4189) 2007 orchard Only protein-protein interactions imex curation tad2b_drome Transcriptional adapter 2B dADA2b Ada2b CG9638 Ada2S protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTTIADLFTKYNCTNCQDDIQGIRVHCAECENFDLCLQCFAAGAEIGAHQNNHSYQFMDTGTSILSVFRGKGAWTAREEIRLLDAIEQYGFGNWEDISKHIETKSAEDAKEEYVNKFVNGTIGRATWTPAQSQRPRLIDHTGDDDAGPLGTNALSTLPPLEINSDEAMQLGYMPNRDSFEREYDPTAEQLISNISLSSEDTEVDVMLKLAHVDIYTRRLRERARRKRMVRDYQLVSNFFRNRNYAQQQGLTKEQREFRDRFRVYAQFYTCNEYERLLGSLEREKELRIRQSELYRYRYNGLTKIAECTHFEQHAATATHRSTGPYGHGKTDHTHTSNGSHRPPSSSLHSPQPNLRKVEMSSGGEASSNSIAPRNTLHIADPTCSGALLPSKNYLDSCRGSSAATMLQTTGMVMGVTVDSGATTGVTSTATTMANLPTNSAKGSQQHLQPLQQHPQLLQSGNQHKMQNEAAGGGSDQVPSMSLKLRTQLEELKHLPQPPGSELLSHNELDLCKKHNITPTTYLSVKTVCLSGAPSLGSPMETSLRKFFIKCGWLSH 89079A24FF11F381 xmas2_drome Protein xmas-2 xmas-2 CG32562 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAEPRPGGYNYKTLLCRNIPELFLDKYVARSHFGRFGTLVNFVLRPRRMTCTVSYASEDEAARALLDGASFQGHLFDISYADNETAPAQKTEEWVDPDIQAELSALQSGWRNEYGSGKPIKKPQNGSSGSGGSSMLPAIPVGPATAPVSRDRTPAQLRDLENMMRRPAHTSEEKFSVLDARDKLLRLNRTQHKLSGATQGHCADMCPEKERVLREFQRQVAYYELQPGSDELICHERALKQYSRSSADQETPLPHELRNETALHMTMSYLMHEIMDISERQDPQSHMGDWFHFVWDRTRSIRKEITQQELCSLGAVKLVEQCARFHIHCAARLVDADPSVFDSKINAENLTKCLQTLKYMYHDLRIKGVPCPKEAEFRGYIVLLNLADANFLWDIGQLPAELQSCPEVRQAIQFYLALQDTNFVRFFQLLADKDTSYLSACILVNYFTRLRVLGLHRLIQAYRSPRKDEVSSLPLSYIAELLSFASEQEAADFVQHYGLQINEAGRVVLSRMHTVETEYKLPRQYELVEVKRVKSVGEVVSGEPLPPRDLYLNHRPHNSFDDYGMLKSIAWTAKDQLAGMQQEEMQPQMPSQPPAVSKHSDTLFKVPMQPDGTAAGFGVFAAAAVPPASSIDGFSFVLPKSRAQEFQEQAPATQQRRAQEEAKHQALQVAIAAAKKREAELMAIHEAKVAEAERVRQQKLRERQEQQRRQQQELEEQRQREQEKLQLEKERQLKLEQLFFVQQQEREAHKQTRTLELYQEIFQDTLAEICQSEFMSHSRACRSYESMLDSITRDLVERQMEQSIYELGVMRVCIRRWRKYRRTQQEKDTLFNQLPLSFGAENPEGVVNKRSMEDSLRLSRRYRLGEPCDYGKLLAGLEEHSWLKLDLWHVLDKCLPVAQPGARRFYKLLISLSGGQEGLQLNCDLDRGLLQQPQSPDARFVDGGYIRGFSQGIGLSVMKIRDDDHDWKATDLAEANGIICLIGLDDIRLLPDRLKPLLQASRCHDVAVIVQHPANTAFVQPDIPLQELCLRSFNIFRLRKSGNNRQRLMIALESAVKFLAKATERKRVGHLHQVETREYLLVNLGSELFRRLKYAAEHDTAIRSDTQLNPQRCVDLFNEAVHRLQLVAGEDLSDWPQFPEELRVFVQPLPIESSLNTNRLEHFEPGWHLPERRQRIVQLLERCKLPKMPVLPRSSSLAEAQCQWVLDYAQISQQEDCVEQIALQAIKILQYDTDDYLNFVEYLAGERMQYILRQERNPPQGIVYNTKTLKRRFLSAWYYEFREPQIYEPVPAEENAQMLEKQPSSQAEVQQLDFDEITSKAEAVLKRFHQRQDERHTLRELNRSHKSRKRRDASDHKHASKHKRKRSKSK 0F3610BB4196E640 taf10_drome Transcription initiation factor TFIID subunit 10 Taf10 Transcription initiation factor TFIID 24 kDa subunit dTAF(II)24 CG2859 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MASDGEDISVTPAESVTSATDTEEEDIDSPLMQSELHSDEEQPDVEEVPLTTEESEMDELIKQLEDYSPTIPDALTMHILKTAGFCTVDPKIVRLVSVSAQKFISDIANDALQHCKTRTTNIQHSSGHSSSKDKKNPKDRKYTLAMEDLVPALADHGITMRKPQYFV DIP protein Q9U5W9 original sequence version: 70 8C8DDCD3BEAEA735 eny2_drome Enhancer of yellow 2 transcription factor e(y)2 ENY2 CG15191 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSTSGAVDQYTVLTGDRSKIKDLLCSRLTECGWRDEVRLMCRNILMEKGTNNSFTVEQLIAEVTPKARTLVPDAVKKELLMKIRTILTEIEEEPDEPEDES FC55DCBA1D2BAFCB tra1_drome Transcription-associated protein 1 Tra1 dTRA1 CG2905 Nipped-A protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSVIENVPVNTFRNYLNILNDSSSKDELKLKATQELSEHFEMIMQSPAYPSFLDNSLKIFMRILQDGEPQFIQENTMQHIRKLILEMIHRLPITESLRQHVKTIITMMLKILKTDNEENVLVCLRIIIELHKHFRPSFNSEIQLFLGFVKEIYTNLPNHLTSIFETSNDVWVTDLKDLNLEVLLSESYSVRTIHVEKALDSNSQQQIYNLLPRGILSLKVLQELPIIVVLMYQIYKNAVHQEVSEFIPLILTTINLQPTVTRRNSPQKEIYVEFMGAQIKTLSFLAYIVRIFQEVVIASSLSVTSGMLNLMKNCPKEAAHLRKELLIAARHIFATDLRQKFIPSIEQLFDEDLLIGKGVTLDSIRPLAYSTLADLAHHVRQSLNIDVLIKAVNLFSKNVHDESLAVGIQTMSCKLLLNLVDCLRHHSETEPQRSKALLSKLLKVFVKKFETIAKIQLPLIIQKCKGHAFSGALVNSSGNASLSHINAPDLKDDISNIQVSASGSQWIYSVNVAEFRSLVKTLVGGVKTITWGFFNSKFQLTDTKLANHEKIFGPEIVCSYIDLVYYAMEALDIYTINVNPNQQRTSGLISRSKEEKEVLEHFSGIFLMMHSQNFQEIFSTTINFLVERIYKNQSLQVIANSFLANPTTSPLFATVLVEYLLNKMEEMGSNLERSNLYLRLFKLVFGSVSLFPVENEQMLRPHLHKIVNRSMELALISEEPYNYFLLLRALFRSIGGGSHDLLYQEFLPLLPNLLEGLNRLQSGFHKQHMRDLFVELCLTVPVRLSSLLPYLPMLMDPLVSALNGSPTLISQGLRTLELCVDNLQPDFLYDHIQPVRAALMQALWKTLRNQDNAALVAFRVLGKFGGGNRKMMVEPQALSYIINDKPTISIVTYFQEYETPIDFPVDEAIKSAFRALGSNSTDQFYRRQSWEVIRCFLAAFISLDDEKHMLLKLFTHVDFVENKIMNWSTFQHKAGNETVRETHQTALIGMLVASATKDLRDSVCPVMAAVVRHYTMVAIAQQAGPFPQKGYQATHGIDPMILIDALASCMGHEEKELCKPGIACMGIILDTATNIMGNKDRACKLPIIQYLAEKMVSLCYDRPWYSKVGGCQAIQFLCKHMSLRALFQNLFNFLKAFMFVLMDLEGDVSNGAIEITKSYMKSMLEICLTPINECYKNIDLKDLQAKATYEVIHELVRHITSPNTIVREESMVLLKHIGTIQSKTVSEVMDPHKDVLADIIPPKKHLLRHQPANAQIGLMDGNTFCTTLEPRLFTIDLTNTYHKLFFHELLTLSEAEDATLAKLDCYKNVPNLIPLRTSALRALAACHYISDIGYKEKIINIIFKVMESDKSELQTTAFHCMKHFITGVTLEKEKVQSAMRPLLLKLGDHRNLSIPAIKRLSYFTQIFPQMFNEKLSEQILQHCSKIMEIFVSEYKSTSPNVNFFASSKGGEYEQKIVILIEMFFYISASVKYIEKLCQLVLKTEKNLMIEASSPYREALIKFLQRFPTETVDLFLTESLMIDPQWNRLFIYLLKHETGVSFRAVIKSSRYNNLIHYLNTHTEFPEALKYEIQHQAVLIIFTLMESDDQWIPTRQDIVDALKNCWQNYLSTLSSEDVLCDLWHLIGKILLHYFSNNTNDIELLFQLLRALCFRFIPDVYFLRDFLQHTVAQSFTVNWKRNAFFYFVENFNNSFLSEELKAKIITAVIIPCFAVSFDKGEGNKLIGAPPTPYQEDEKNIVSVFINKVFDPDKQYDDAVRIALLQLACLLVERASQHIHDGDANNKRQGNKLRRLMTFAWPCLLSKSSVDPTARYHGHLLLSHIIARLAIHKKIVLQVFHSLLKGHALEARSIVKQALDVLTPAMPLRMEDGNTMLTHWTKKIIVEEGHAMQQLFHILQLIIRHYKVYFPVRHQLVQHLINYMQRLGFPPTASIEHKKLAVDLAEVIIKWELHRIKDDRETKTDGTEEELIQESSVKRSGIDLVETRKKSFDIIRETTVQGVGSHTKPDDILRSIDKSYCDTVLNFLIRLACQVNDPQAPILSPGESLSRRCVMLLKMAMRPEIWPQPFDIKLNWLDKVLATVETPHHNLNNICTGIDFLTFLTTILSPDQLVSIIRPVQRGLSLCIIHQNTRIVRLMHMFLTRIMAIFPPDTQHKHEDLDLLYTAVSKMIAENLTSYEKSPQPNASSLFGTLMILKACTTNNASYIDRILVQFIRVLNHLTRDHINTIGGNTVISQSPDSNALPLELLVLSLELIKNRIFVMSVEIRKLFIGTILVSLIEKSTEVKIIKCIIKMLDEWIKTKEPNVMTQVPSIREKSALLVKLMQNVEKKFTDEIELNIQFLEIINFIYRDEILKQTELTNKLEGAFLNGLRFQNPNVRSKFFEILDSSMRRRLHDRLLYIICSQAWDTIGSHYWIKQCIELLILTANTMMQIQCSNEQFKIPSITSVIPVNSSETQENSFVSFLSSHSESFDIIQTVDDKDDVYDIDLNADRKEDCQQILPNRRVTLVELVYKQAEFLEANRNIRTDQMLVATSQLCHIDTQLAQSVWLSMFPRIWSIFTEDQRCNITKELIPFLSSGTNVNQKDCHPSTLNTFVESLTKCAPPIYIPPNLLAYLGKSHNLWHRAILVLEDMAVNQSMQSKDIDGGENQFSDLDVQQSNNIFDSLSKMYSSMHEEDLWAGLWLKFAHYPETNIAVSYEQMGFFEEAQGAYDLAMTKFKQDLSNGVVNTYVNSELLLWENHWMRCAKELNQWDILLDYAQTNKDKNMFLILESSWRVPDWNLMKIALAKTEQCYLKHYGFKINLYKGYLSILHQEERQTGNIERYVEIASSLCIREWRRLPNIVSHIHLPYLQASQQIMELHEASQIHQGLAQSRNNSLHDMKAIVKTWRNRLPIISDDLSHWSDIFTWRQHHYQIITQHLEQQSDQGSTMLGVHASAQAIISFGKIARKHNLTGVCQETLSRIYTIPSVPIVDCFQKIRQQVKCYLQMPSTSGKNEINEALEVIESTNLKYFTGEMNAEFYALKGLLLAQIGRSEEAGKSFSVAAQLHDGLTKAWAMWGDYMEQIFLKERKITLAVDALICYLQASRNQIESKTRKYIAKVLWFLSYDNNTKILISTLEKHVAGIPPSYWLPWIPQLLCCLEQFEGDVILNLLSQIGRLYPQAVYFPIRTLYLTLKIEQREKHKTAEQAVKSSCSNIDGTTLSFGRGASHGNIPSINPIKATPPMWRCSKVMQLQREVHPTILSSLEGIVDQMVWFRESWTEEVLRQLRQGLIKCYAIAFEKRDTVQHSTITPHTLHFVKKLGSTFGIGIENVPGSVTSSISNSAASESLARRAQVTFQDPVFQKMKEQFTNDFDFSKPGAMKLHNLISKLKTWIKVLETKVKKLPTSFLIEDKCRFLSNFSQKTAEVELPGELLIPLSSHYYVRIARFMPRVEIVQKNNTAARRLYIRGTNGKIYPYLVVLDSGLGDARREERVLQLKRMLNYYLEKQKETSRRFLNITVPRVVPISPQMRLAEDNPNSISLLKIFKKCCQSMQVDYDMPIVKYYDRLSEVQARGTPTTHTLLREIFSEIQWTMVPKTLLKHWALKTFLAATDFWHFRKMLTLQLALAFLCEHALNLTRLNADMMYLHQDSGLMNISYFKFDVNDDKCQLNQHRPVPFRLTPNVGEFITHFGITGPLSAAIVATARCFIQPNYKLSSILQTILRDEIIALQKKGFRECKLIEGSEDRYSDGNCMEHSVNIVNSAVDIIMTRFNKISYFDSIENKKISVLVQSATNIDNLCRMDPAWHPWL 366CF25BBC7C5435 nup62_drome Nuclear pore glycoprotein p62 Nup62 62 kDa nucleoporin CG6251 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MVFQLPTTTAAPTGGATSSFSFGLSTGTPAAAPASGAATTAPATKTTFSFGTPAPTAGIGGGDADNSKAQAPPAFGFGLGSGTASAPLTLGTQAAANPASTTSATATGTSAAPPAFGGFTAQPAASVVPTIATSAPNTAATTTGLLGGSGLGAPKTTAAASTTLTAAPSAIASTQGAAPAPTLSTGGAFANLTTETKTTDSSAVSTASQLSYHQLEEHINKWTLEFEEQEKVFTEQATQINAWDKLLISNNGKIVELNDAVKKVKTDQQVLDQELEFIATQQKELEDSLGPLEKEFVNLPRVDMERSQTYLMVENLDTQLKQMSEDLKEIIDNLNEANKGQDTTDPIIQIGKILNAHMNSLQWIESQSTNISKKLEDIGKIQDSQKRDIFRAPF 6D3C1E3A149ECCE6 o76216_drome Histone acetyltransferase Gcn5 Pcaf CG4107 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSGGPSITIKSQPIDGNNTGNAAAQQQQQAANGAATAGASGAAGSAQNPGHGGAASGAGSVPAEGTRQNSLQRIQQRKQKVFNLPVPQKLAKLSMYSACQSEGCRCTGWKTPQENRHRDVESSYCPEFNEECRNTSCRHSLRSHIAHLDNISSSSMNELLGAIIDMENLFMSMQRVEDEDTKKVYQYLFRLLRQCVLTRQQAVIRGPLGDPPFETPCITKAVLSLVFYKYNHLSTPELQTMTEVAKTFLNFLNHYNFESPSTRRGDLTHEDASNYKINYTRWLVFCHVPAFCNSLRQCETSLVFGRTLLRTVFQCVSQQLKKKCISERDRFPEDKRSIITLMPKFLETLRAELLKDDSPIWDTSYRPSNSFVIQQRKRNQEVASVPIGPSAASIGGNKRTSVGEPLHKRIKKEPTDRPSSENLDDLPADVVMRAMKSVSESKTTNKAEILFPVNVSRDENVKAEEQKRAIEFHVVGNSLTKPVDKQTVLWLLGLQLVFAYQLPEMPREYISQLVFDTKHKTLALIKENQPIGGICFRPFPSQGFTEIVFCAVTMSEQVKGYGTHLMNHLKDYSIQRGIKHLLTFADCDAIGYFKKQGFSKDVKLARPVYAGYIKEYDSATLMHCELHPSIVNTQFIAVIRSQSEILKELIAQRHNEVQKVRPGLTCFKEGLPVIPVESIPGLREIGWKPQNRPARSSRPLEESTDPEKLATSFASVLQSVRQHTTAWPFLRPVTAAEVPDYYDHIKYPMDLKTMGERLKKGYYQTRRLFMADMARIFSNCRFYNSPDTEYYRCANSLERYFQTKMRELGLWDK 7983E218CBB98D93 ey2-ada2b-1 3 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey association association Fig. 1. A The anti-E(y)2 immunoprecipitation (IP) co-purified known dSAGA/TFTC subunits, such as dTRRAP, dGCN5, dADA2b and dTAF10 specific polyclonal antibodies against GCN5, E(Y)2, TRRAP, ADA2b and TAF10 gcn5-ey2-1 3 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait association association Fig. 1. A the anti-GCN5 IP co-purified E(y)2 together with other known dSAGA/TFTC subunits specific polyclonal antibodies against GCN5, E(Y)2, TRRAP, ADA2b and TAF10 ey2-gcn5-1 4 8 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 1. B Anti-E(y)2 antibody staining revealed that E(y)2 associates with a large number of loci(Figure 1B) and that many of these loci were also stained by the anti-dGCN5 antibody (see merges in Figure 1B), indicat- ing a colocalization between GCN5 and E(y)2 specific antibodies against E(Y)2 and GCN5 ey2-xmas2-1 1 8 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 3. A In this analysis, using the Drosophila cDNA encoding the full-length E(y)2 as a bait, one of the isolated positive cDNA clones encoded the Drosophila Xmas-2 ey2-nup62-1 3 8 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Fig. 2.C The anti-E(y)2 antibody co-purified NPC components with E(y)2 (Figure 2C, lane 3), indicating that E(y)2-containing complexes can be found in contact with NPC components in the cells specific polyclonal antibodies against E(Y)2 and nucleoporin p62 (Mab414) gcn5-nup62-2 3 11 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Fig. 2.C The anti-GCN5 antibody co-immunoprecipitated NPC components specific polyclonal antibodies against GCN5 and nucleoporin p62 (Mab414) The anti-GCN5 co-immunoprecipitated nucleoporin p62 to a lesser extent than the anti-E(Y)2 coip. ey2-nup62-2 2 8 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 2. B When proteins of the NPC and E(y)2 were stained, colocalization was observed at the nuclear periphery specific antibodies against E(Y)2 and nucleoporin p62 (Mab414) After RNAi of Xmas-2 a significant fraction of E(y)2 looses its peripheral localization with nucleophorin p62 gcn5-nup62-1 2 11 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 2. B When typical SAGA/TFTC subunits, such as GCN5 and ADA2b, were co-stained with NPC components (Figure B, panels g�i and k), a colocalization between the two complexes could also be detected in some regions close to the nuclear envelope specific antibodies against GCN5 and nucleoporin p62 (Mab414) ada5b-nup62-1 2 10 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 2. B When typical SAGA/TFTC subunits, such as GCN5 and ADA2b, were co-stained with NPC components (Figure B, panels g�i and k), a colocalization between the two complexes could also be detected in some regions close to the nuclear envelope specific antibodies against ADA5b and nucleoporin p62 (Mab414) ey2-xmas2-2 3 8 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Fig. 3. B The anti-E(y)2 IP co-purified Xmas-2 specific polyclonal antibodies against E(Y)2 and xmas-2 not all cellular Xmas-2 co-precipitated with E(y)2. It is suggering that in the cells both E(y)2 and Xmas-2 are present either as free proteins or as subunits of other complexes xmas2-ey2-1 3 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Fig. 3. B the anti-Xmas-2 IP co-precipitated E(y)2 specific polyclonal antibodies against E(Y)2 and xmas-2 not all E(y)2 co-immunoprecipitated with Xmas-2. It is suggering that in the cells both E(y)2 and Xmas-2 are present either as free proteins or as subunits of other complexes ey2-xmas2-3 4 6 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 3. C in vivo colocalization between Xmas-2 and E(y)2 specific antibodies against E(Y)2 and xmas-2 ey2-xmas2-4 2 8 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 3. E Xmas-2 and E(y)2 colocalize at many, but not all sites on the nuclear periphery specific antibodies against E(Y)2 and xmas-2 xmas2-nup62-1 2 10 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 3. E the localization of Xmas-2 on the nuclear periphery of S2 cells also often coincides with that of NPCs specific antibodies against xmas-2 and nucleoporin p62 (Mab414) xmas2-nup62-2 3 6 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Fig. 3. D the anti-Xmas-2 antibody co-immunoprecipitated NPC components specific polyclonal antibodies against xmas-2 and nucleoporin p62 (Mab414)