DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu A protein phosphorylation/dephosphorylation network regulates a plant potassium channel. A protein phosphorylation/dephosphorylation network regulates a plant potassium channel. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2007 Lee SC., Lan WZ., Kim BG., Li L., Cheong YH., Pandey GK., Lu G., Buchanan BB., Luan S. sluan@nature.berkeley.edu,view@nature.berkeley.edu 17-April-2008: Contacted by JYOTI. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lee SC., Lan WZ., Kim BG., Li L., Cheong YH., Pandey GK., Lu G., Buchanan BB., Luan S. sluan@nature.berkeley.edu,view@nature.berkeley.edu Accepted 2008-MAR-6 by CDEROW. 17-April-2008: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2007 cnbl3_arath Calcineurin B-like protein 3 SOS3-like calcium-binding protein 6 CBL3 SCABP6 At4g26570 T15N24.20 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSQCIDGFKHVCSSFFRCFDIDIYKQSGGLGDPELLARETVFSVSEIEALYELFKKISSAVIDDGLINKEEFQLALFKTNKKESLFADRVFDLFDTKHNGILGFEEFARALSVFHPNAPIEDKIDFSFQLYDLKQQGFIERQEVKQMVVATLAESGMNLSDEIIESIIDKTFEEADTKHDGRIDKEEWRTLVLRHPSLLKNMTLQYLKDITTTFPSFVFHSQVEDT F78222E98F64983E cnbl9_arath Calcineurin B-like protein 9 CBL9 At5g47100 K14A3.5 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGCFHSTAAREFPDHENPVKLASETAFSVSEVEALYELFKSISSSVVDDGLINKEEFQLALFKNRKKENLFANRIFDLFDVKRKGVIDFGDFVRSLNVFHPNASLEEKTDFTFRLYDMDCTGFIERQEVKQMLIALLCESEMKLADDTIEMILDQTFEDADVDRDGKIDKTEWSNFVIKNPSLLKIMTLPYLRDITTTFPSFVFNSEVDEIAT E848102CD14211EE cipkg_arath CBL-interacting serine/threonine-protein kinase 16 PKS15 SnRK3.18 SOS2-like protein kinase PKS15 SNF1-related kinase 3.18 F13D4.161 CIPK16 At2g25090 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEESNRSSTVLFDKYNIGRLLGTGNFAKVYHGTEISTGDDVAIKVIKKDHVFKRRGMMEQIEREIAVMRLLRHPNVVELREVMATKKKIFFVMEYVNGGELFEMIDRDGKLPEDLARKYFQQLISAVDFCHSRGVFHRDIKPENLLLDGEGDLKVTDFGLSALMMPEGLGGRRGSSDDLLHTRCGTPAYVAPEVLRNKGYDGAMADIWSCGIVLYALLAGFLPFIDENVMTLYTKIFKAECEFPPWFSLESKELLSRLLVPDPEQRISMSEIKMIPWFRKNFTPSVAFSIDETIPSPPEPPTKKKKKDLNEKEDDGASPRSFNAFQFITSMSSGFDLSNLFEIKRKPKRMFTSKFPAKSVKERLETAAREMDMRVKHVKDCKMKLQRRTEGRKGRLSVTAEVFEVAPEVSVVEFCKTSGDTLEYYLFCEDDVRPALKDIVWSWQGDDDEDDVTTNDNVDTNDNKINNVS 460E17225D6A7A7A cnbl1_arath Calcineurin B-like protein 1 SOS3-like calcium-binding protein 5 CBL1 SCABP5 At4g17615 dl4845w FCAALL.122 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGCFHSKAAKEFRGHEDPVKLASETAFSVSEVEALFELFKSISSSVVDDGLINKEEFQLALFKSRKRENIFANRIFDMFDVKRKGVIDFGDFVRSLNVFHPNASLEDKIDFTFRLYDMDCTGYIERQEVKQMLIALLCESEMKLADETIEIILDKTFEDADVNQDGKIDKLEWSDFVNKNPSLLKIMTLPYLRDITTTFPSFVFHSEVDEIAT 1C310FC449D027B1 akt1_arath Potassium channel AKT1 AKT1 At2g26650 F18A8.2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MRGGALLCGQVQDEIEQLSRESSHFSLSTGILPSLGARSNRRVKLRRFVVSPYDHKYRIWEAFLVVLVVYTAWVSPFEFGFLRKPRPPLSITDNIVNAFFAIDIIMTFFVGYLDKSTYLIVDDRKQIAFKYLRSWFLLDLVSTIPSEAAMRISSQSYGLFNMLRLWRLRRVGALFARLEKDRNFNYFWVRCAKLVCVTLFAVHCAACFYYLIAARNSNPAKTWIGANVANFLEESLWMRYVTSMYWSITTLTTVGYGDLHPVNTKEMIFDIFYMLFNLGLTAYLIGNMTNLVVHGTSRTRNFRDTIQAASNFAHRNHLPPRLQDQMLAHLCLKYRTDSEGLQQQETLDALPKAIRSSISHFLFYSLMDKVYLFRGVSNDLLFQLVSEMKAEYFPPKEDVILQNEAPTDFYILVNGTADLVDVDTGTESIVREVKAGDIIGEIGVLCYRPQLFTVRTKRLCQLLRMNRTTFLNIIQANVGDGTIIMNNLLQHLKEMNDPVMTNVLLEIENMLARGKMDLPLNLCFAAIREDDLLLHQLLKRGLDPNESDNNGRTPLHIAASKGTLNCVLLLLEYHADPNCRDAEGSVPLWEAMVEGHEKVVKVLLEHGSTIDAGDVGHFACTAAEQGNLKLLKEIVLHGGDVTRPRATGTSALHTAVCEENIEMVKYLLEQGADVNKQDMHGWTPRDLAEQQGHEDIKALFREKLHERRVHIETSSSVPILKTGIRFLGRFTSEPNIRPASREVSFRIRETRARRKTNNFDNSLFGILANQSVPKNGLATVDEGRTGNPVRVTISCAEKDDIAGKLVLLPGSFKELLELGSNKFGIVATKVMNKDNNAEIDDVDVIRDGDHLIFATDS DIP protein Q38998 original sequence version: 122 DIP protein Q38998 original sequence version: 69 28E1622BA3505F4C p2c03_arath Protein phosphatase 2C 3 AIP1 Protein AKT1-INTERACTING 1 F22G5.22 At1g07430 Protein phosphatase 2C AIP1 HAI2 HON Protein HIGHLY ABA-INDUCED PP2C 2 Protein HONSU protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MADICYEDETSACESRPLWSSRKWRIGVQRFRMSPSEMNPTASTTEEEDKSEGIYNKRNKQEEYDFMNCASSSPSQSSPEEESVSLEDSDVSISDGNSSVNDVAVIPSKKTVKETDLRPRYGVASVCGRRRDMEDAVALHPSFVRKQTEFSRTRWHYFGVYDGHGCSHVAARCKERLHELVQEEALSDKKEEWKKMMERSFTRMDKEVVRWGETVMSANCRCELQTPDCDAVGSTAVVSVITPEKIIVANCGDSRAVLCRNGKAVPLSTDHKPDRPDELDRIQEAGGRVIYWDGARVLGVLAMSRAIGDNYLKPYVTSEPEVTVTDRTEEDEFLILATDGLWDVVTNEAACTMVRMCLNRKSGRGRRRGETQTPGRRSEEEGKEEEEKVVGSRKNGKRGEITDKACTEASVLLTKLALAKHSSDNVSVVVIDLRRRRKRHVA DIP protein Q9LNW3 original sequence version: 51 DIP protein Q9LNW3 original sequence version: 65 DIP protein Q9LNW3 original sequence version: 113 892DB70AB83312AD cipkn_arath CBL-interacting serine/threonine-protein kinase 23 LKS1 PKS17 SnRK3.23 SOS2-like protein kinase PKS17 SNF1-related kinase 3.23 Protein LOW-K(+)-SENSITIVE 1 F12P21.6 CIPK23 At1g30270 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASRTTPSRSTPSRSTPSGSSSGGRTRVGKYELGRTLGEGTFAKVKFARNVENGDNVAIKVIDKEKVLKNKMIAQIKREISTMKLIKHPNVIRMFEVMASKTKIYFVLEFVTGGELFDKISSNGRLKEDEARKYFQQLINAVDYCHSRGVYHRDLKPENLLLDANGALKVSDFGLSALPQQVREDGLLHTTCGTPNYVAPEVINNKGYDGAKADLWSCGVILFVLMAGYLPFEDSNLTSLYKKIFKAEFTCPPWFSASAKKLIKRILDPNPATRITFAEVIENEWFKKGYKAPKFENADVSLDDVDAIFDDSGESKNLVVERREEGLKTPVTMNAFELISTSQGLNLGSLFEKQMGLVKRKTRFTSKSSANEIVTKIEAAAAPMGFDVKTNNYKMKLTGEKSGRKGQLAVATEVFQVAPSLYMVEMRKSGGDTLEFHKFYKNLTTGLKDIVWKTIDEEKEEGTDGGGTNGAMANRTIAKQST DIP protein Q93VD3 original sequence version: 98 DIP protein Q93VD3 original sequence version: 45 CAA1B526B955F5F2 cipk6_arath CBL-interacting serine/threonine-protein kinase 6 SOS2-like protein kinase PKS4 SNF1-related kinase 3.14 SOS3-interacting protein 3 CIPK6 PKS4 At4g30960 SIP3 SnRK3.14 F6I18.130 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVGAKPVENGSDGGSSTGLLHGRYELGRLLGHGTFAKVYHARNIQTGKSVAMKVVGKEKVVKVGMVDQIKREISVMRMVKHPNIVELHEVMASKSKIYFAMELVRGGELFAKVAKGRLREDVARVYFQQLISAVDFCHSRGVYHRDLKPENLLLDEEGNLKVTDFGLSAFTEHLKQDGLLHTTCGTPAYVAPEVILKKGYDGAKADLWSCGVILFVLLAGYLPFQDDNLVNMYRKIYRGDFKCPGWLSSDARRLVTKLLDPNPNTRITIEKVMDSPWFKKQATRSRNEPVAATITTTEEDVDFLVHKSKEETETLNAFHIIALSEGFDLSPLFEEKKKEEKREMRFATSRPASSVISSLEEAARVGNKFDVRKSESRVRIEGKQNGRKGKLAVEAEIFAVAPSFVVVEVKKDHGDTLEYNNFCSTALRPALKDIFWTSTPA 469728C6CB83F428 cnbl2_arath Calcineurin B-like protein 2 CBL2 SCABP1 At5g55990 MDA7.3 SOS3-like calcium-binding protein 1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSQCVDGIKHLCTSVLGCFDLDLYKQSGGLGDPELLARDTVFSVSEIEALYELFKKISSAVIDDGLINKEEFQLALFKTNKKESLFADRVFDLFDTKHNGILGFEEFARALSVFHPNAPIDDKIHFSFQLYDLKQQGFIERQEVKQMVVATLAESGMNLKDTVIEDIIDKTFEEADTKHDGKIDKEEWRSLVLRHPSLLKNMTLQYLKDITTTFPSFVFHSQVEDT 342A013FC28D1445 akt1-cipk6 1 6 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 1A, 3B, 4B These experiments revealed that, in addition to CIPK23, at least two other CIPKs, CIPK6 and CIPK16, interacted with the C terminus of AKT1 akt1-cipk23 1 8 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 1A, 3A, 4A These experiments revealed that, in addition to CIPK23, at least two other CIPKs, CIPK6 and CIPK16, interacted with the C terminus of AKT1 akt1-cipk16 1 4 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 1A, 3C, 4C These experiments revealed that, in addition to CIPK23, at least two other CIPKs, CIPK6 and CIPK16, interacted with the C terminus of AKT1 cipk6-cbl1 1 9 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Figure 1B Interestingly, despite the low overall sequence similarity in the three CIPK proteins, they interacted with the same members of the CBL family,including CBL1, CBL2, CBL3, and CBL9 cipk6-cbl2 1 9 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Figure 1B Interestingly, despite the low overall sequence similarity in the three CIPK proteins, they interacted with the same members of the CBL family,including CBL1, CBL2, CBL3, and CBL9 cipk6-cbl3 1 2 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association Figure 1B Interestingly, despite the low overall sequence similarity in the three CIPK proteins, they interacted with the same members of the CBL family,including CBL1, CBL2, CBL3, and CBL9 cipk6-cbl9 1 3 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association Figure 1B Interestingly, despite the low overall sequence similarity in the three CIPK proteins, they interacted with the same members of the CBL family,including CBL1, CBL2, CBL3, and CBL9 cipk23-cbl3-1 1 2 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Figure 1B Interestingly, despite the low overall sequence similarity in the three CIPK proteins, they interacted with the same members of the CBL family,including CBL1, CBL2, CBL3, and CBL9 cipk23-cbl2-1 1 10 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Figure 1B Interestingly, despite the low overall sequence similarity in the three CIPK proteins, they interacted with the same members of the CBL family,including CBL1, CBL2, CBL3, and CBL9 cipk23-cbl1-2 1 5 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Figure 1B Interestingly, despite the low overall sequence similarity in the three CIPK proteins, they interacted with the same members of the CBL family,including CBL1, CBL2, CBL3, and CBL9 cipk23-cbl9-2 1 8 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association Figure 1B Interestingly, despite the low overall sequence similarity in the three CIPK proteins, they interacted with the same members of the CBL family,including CBL1, CBL2, CBL3, and CBL9 cipk16-cbl3-1 1 2 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Figure 1B Interestingly, despite the low overall sequence similarity in the three CIPK proteins, they interacted with the same members of the CBL family,including CBL1, CBL2, CBL3, and CBL9 cipk16-cbl2-1 1 4 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Figure 1B Interestingly, despite the low overall sequence similarity in the three CIPK proteins, they interacted with the same members of the CBL family,including CBL1, CBL2, CBL3, and CBL9 cipk16-cbl1-1 1 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Figure 1B Interestingly, despite the low overall sequence similarity in the three CIPK proteins, they interacted with the same members of the CBL family,including CBL1, CBL2, CBL3, and CBL9 cipk16-cbl9-1 1 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Figure 1B Interestingly, despite the low overall sequence similarity in the three CIPK proteins, they interacted with the same members of the CBL family,including CBL1, CBL2, CBL3, and CBL9 akt1-at1g07430 1 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Figure 6A Among the five PP2CA subfamily members tested (ABI1, ABI2, HAB1, HAB2, and a novel member, At1g07430), only the novel member interacted with AKT1 cipk23-at1g07430-1 1 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Figure 6A The yeast two-hybrid results indicated that AIP1 (but not other PP2CAs) interacted with CIPK23 that also interacts with AKT1.