UniProt UniProt http://www.uniprot.org IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex. IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex. J. Immunol. (0022-1767) 2007 imex curation Chackerian AA., Oldham ER., Murphy EE., Schmitz J., Pflanz S., Kastelein RA. affinity of the st2-il33 interaction was measured, p. 2553, but data not shown. IL-33 or IL-1beta was titrated into wells containing plate-bound IL-1RL1/ST2 (or IL-1R1) and soluble his6-tagged IL-1RAP. Complex formation was detected with an anti-his6 antibody as the concentration of the cytokine (IL-33 or IL-1beta) increases. rob.kastelein@sporp.com 27-Aug-2008: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Chackerian AA., Oldham ER., Murphy EE., Schmitz J., Pflanz S., Kastelein RA. affinity of the st2-il33 interaction was measured, p. 2553, but data not shown. IL-33 or IL-1beta was titrated into wells containing plate-bound IL-1RL1/ST2 (or IL-1R1) and soluble his6-tagged IL-1RAP. Complex formation was detected with an anti-his6 antibody as the concentration of the cytokine (IL-33 or IL-1beta) increases. rob.kastelein@sporp.com Accepted 2008-AUG-17 by CDEROW. J. Immunol. (0022-1767) 2007 Only protein-protein interactions imex curation il1r1_mouse Interleukin-1 receptor type 1 Il1r1 Il-1r1 Il1ra Interleukin-1 receptor type I p80 CD121 antigen-like family member A Interleukin-1 receptor alpha protein protein mouse Mus musculus Mouse MENMKVLLGLICLMVPLLSLEIDVCTEYPNQIVLFLSVNEIDIRKCPLTPNKMHGDTIIWYKNDSKTPISADRDSRIHQQNEHLWFVPAKVEDSGYYYCIVRNSTYCLKTKVTVTVLENDPGLCYSTQATFPQRLHIAGDGSLVCPYVSYFKDENNELPEVQWYKNCKPLLLDNVSFFGVKDKLLVRNVAEEHRGDYICRMSYTFRGKQYPVTRVIQFITIDENKRDRPVILSPRNETIEADPGSMIQLICNVTGQFSDLVYWKWNGSEIEWNDPFLAEDYQFVEHPSTKRKYTLITTLNISEVKSQFYRYPFICVVKNTNIFESAHVQLIYPVPDFKNYLIGGFIILTATIVCCVCIYKVFKVDIVLWYRDSCSGFLPSKASDGKTYDAYILYPKTLGEGSFSDLDTFVFKLLPEVLEGQFGYKLFIYGRDDYVGEDTIEVTNENVKKSRRLIIILVRDMGGFSWLGQSSEEQIAIYNALIQEGIKIVLLELEKIQDYEKMPDSIQFIKQKHGVICWSGDFQERPQSAKTRFWKNLRYQMPAQRRSPLSKHRLLTLDPVRDTKEKLPAATHLPLG DIP protein P13504 original sequence version: 148 7AA8304C86412A16 il33_mouse Interleukin-33 Il33 protein protein mouse Mus musculus Mouse MRPRMKYSNSKISPAKFSSTAGEALVPPCKIRRSQQKTKEFCHVYCMRLRSGLTIRKETSYFRKEPTKRYSLKSGTKHEENFSAYPRDSRKRSLLGSIQAFAASVDTLSIQGTSLLTQSPASLSTYNDQSVSFVLENGCYVINVDDSGKDQEQDQVLLRYYESPCPASQSGDGVDGKKLMVNMSPIKDTDIWLHANDKDYSVELQRGDVSPPEQAFFVLHKKSSDFVSFECKNLPGTYIGVKDNQLALVEEKDESCNNIMFKLSKI E03C2C297EB43E23 il1ap_mouse Interleukin-1 receptor accessory protein Il1rap Interleukin-33 receptot beta chain protein protein mouse Mus musculus Mouse MGLLWYLMSLSFYGILQSHASERCDDWGLDTMRQIQVFEDEPARIKCPLFEHFLKYNYSTAHSSGLTLIWYWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTCMLRNTTYCSKVAFPLEVVQKDSCFNSAMRFPVHKMYIEHGIHKITCPNVDGYFPSSVKPSVTWYKGCTEIVDFHNVLPEGMNLSFFIPLVSNNGNYTCVVTYPENGRLFHLTRTVTVKVVGSPKDALPPQIYSPNDRVVYEKEPGEELVIPCKVYFSFIMDSHNEVWWTIDGKKPDDVTVDITINESVSYSSTEDETRTQILSIKKVTPEDLRRNYVCHARNTKGEAEQAAKVKQKVIPPRYTVELACGFGATVFLVVVLIVVYHVYWLEMVLFYRAHFGTDETILDGKEYDIYVSYARNVEEEEFVLLTLRGVLENEFGYKLCIFDRDSLPGGIVTDETLSFIQKSRRLLVVLSPNYVLQGTQALLELKAGLENMASRGNINVILVQYKAVKDMKVKELKRAKTVLTVIKWKGEKSKYPQGRFWKQLQVAMPVKKSPRWSSNDKQGLSYSSLKNV DIP protein Q61730 original sequence version: 73 4D4B07E0310AFDC5 ilrl1_mouse Interleukin-1 receptor-like 1 Protein ST2 Protein T1 Lymphocyte antigen 84 Il1rl1 Ly84 St2 Ste2 Interleukin-33 receptor alpha chain protein protein mouse Mus musculus Mouse MIDRQRMGLWALAILTLPMYLTVTEGSKSSWGLENEALIVRCPQRGRSTYPVEWYYSDTNESIPTQKRNRIFVSRDRLKFLPARVEDSGIYACVIRSPNLNKTGYLNVTIHKKPPSCNIPDYLMYSTVRGSDKNFKITCPTIDLYNWTAPVQWFKNCKALQEPRFRAHRSYLFIDNVTHDDEGDYTCQFTHAENGTNYIVTATRSFTVEEKGFSMFPVITNPPYNHTMEVEIGKPASIACSACFGKGSHFLADVLWQINKTVVGNFGEARIQEEEGRNESSSNDMDCLTSVLRITGVTEKDLSLEYDCLALNLHGMIRHTIRLRRKQPIDHRSIYYIVAGCSLLLMFINVLVIVLKVFWIEVALFWRDIVTPYKTRNDGKLYDAYIIYPRVFRGSAAGTHSVEYFVHHTLPDVLENKCGYKLCIYGRDLLPGQDAATVVESSIQNSRRQVFVLAPHMMHSKEFAYEQEIALHSALIQNNSKVILIEMEPLGEASRLQVGDLQDSLQHLVKIQGTIKWREDHVADKQSLSSKFWKHVRYQMPVPERASKTASVAAPLSGKACLDLKHF DIP protein P14719 original sequence version: 93 9228CE227D95B0BC il1b_mouse Interleukin-1 beta Il1b protein protein mouse Mus musculus Mouse MATVPELNCEMPPFDSDENDLFFEVDGPQKMKGCFQTFDLGCPDESIQLQISQQHINKSFRQAVSLIVAVEKLWQLPVSFPWTFQDEDMSTFFSFIFEEEPILCDSWDDDDNLLVCDVPIRQLHYRLRDEQQKSLVLSDPYELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSS 734FA17B02ED87EE il33-il1rap 1 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait region ST2-Fc fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 3 il1r1-il1b 1 6 unspecified role unspecified role prey prey 2 unspecified role unspecified role bait bait region IL-1R1-Fc fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 3