IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ SUPPRESSOR OF FRIGIDA4, encoding a C2H2-Type zinc finger protein, represses flowering by transcriptional activation of Arabidopsis FLOWERING LOCUS C. SUPPRESSOR OF FRIGIDA4, encoding a C2H2-Type zinc finger protein, represses flowering by transcriptional activation of Arabidopsis FLOWERING LOCUS C. Plant Cell (1040-4651) 2006 imex curation Kim S., Choi K., Park C., Hwang HJ., Lee I. ilhalee@snu.ac.kr 03-Mar-2009: Contacted by JYOTI. 03-Feb-2009: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kim S., Choi K., Park C., Hwang HJ., Lee I. ilhalee@snu.ac.kr Accepted 2009-FEB-19 by ORCHARD. 03-Mar-2009: Contacted by JYOTI. Plant Cell (1040-4651) 2006 orchard Only protein-protein interactions imex curation SUPPRESSOR OF FRIGIDA4, encoding a C2H2-Type zinc finger protein, represses flowering by transcriptional activation of Arabidopsis FLOWERING LOCUS C. SUPPRESSOR OF FRIGIDA4, encoding a C2H2-Type zinc finger protein, represses flowering by transcriptional activation of Arabidopsis FLOWERING LOCUS C. Plant Cell (1040-4651) 2006 imex curation Kim S., Choi K., Park C., Hwang HJ., Lee I. ilhalee@snu.ac.kr 03-Mar-2009: Contacted by JYOTI. 03-Feb-2009: Contacted by JYOTI. orchard Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Kim S., Choi K., Park C., Hwang HJ., Lee I. ilhalee@snu.ac.kr Accepted 2009-FEB-19 by ORCHARD. 03-Mar-2009: Contacted by JYOTI. Plant Cell (1040-4651) 2006 orchard Only protein-protein interactions imex curation SUPPRESSOR OF FRIGIDA4, encoding a C2H2-Type zinc finger protein, represses flowering by transcriptional activation of Arabidopsis FLOWERING LOCUS C. SUPPRESSOR OF FRIGIDA4, encoding a C2H2-Type zinc finger protein, represses flowering by transcriptional activation of Arabidopsis FLOWERING LOCUS C. Plant Cell (1040-4651) 2006 imex curation Kim S., Choi K., Park C., Hwang HJ., Lee I. ilhalee@snu.ac.kr 03-Mar-2009: Contacted by JYOTI. 03-Feb-2009: Contacted by JYOTI. orchard Only protein-protein interactions nicbe Nicotiana benthamiana anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kim S., Choi K., Park C., Hwang HJ., Lee I. It was not possible to unambiguously identify the protein named SUF3 in this paper. Therefore interactions involving this protein were not curated. ilhalee@snu.ac.kr Accepted 2009-FEB-19 by ORCHARD. 03-Mar-2009: Contacted by JYOTI. Plant Cell (1040-4651) 2006 orchard Only protein-protein interactions imex curation frl1a_arath FRIGIDA-like protein 1 FRL1 Protein SUPPRESSOR OF FRI 8 SUF8 MQK4.4 At5g16320 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTASETIATAINQIDEKKEKLKKAFDDLQAHRSLLSPSFSLSWSEIDSHFSSLQSSLASRFRLLHSTSPLEHDSYRIDASDAGKSSSSEEVSEQPVVEPELRALCEKIDGIGLIKYLIRIWDDETPLNQEVSAAIRYSPDTASMVLDAIEGSNYTPSSSGRSFDVRRVFVLLMEVLIEINANITVDTRNRAKKLAYHWKSKVGVKPFEALVFLHLVAAFELGSEFDTEELSDYVFMIAKYKQATLVCNKIGVDRKRVGKLIKTLLDSGKPILAVKFMYECGMTDEFEPIPVLKSYIKDCREAALRVCVEDNYSLKSQNEASDKEVSALKPLIKIIKDQNLESEFTQEKVEERVEELEKNKALRKRNTTNPPKQEPQQKGKKRTRDCKNGSQVPVPSQQLLSRPEALLMPEHSHHGLQLNPYGLMTSAFSGVVVNPLTGLFGSGATPQSLYYAQQTGYVLPPQYHPPYYSQ 2924997BB01366D6 flc_arath_gene FLC gene encoding flowering locus C protein gene gene arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress lumi_arath Homeobox protein LUMINIDEPENDENS LD T10P11.15 At4g02560 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDAFKEEIEIGSSVESLMELLDSQKVLFHSQIDQLQDVVVAQCKLTGVNPLAQEMAAGALSIKIGKRPRDLLNPKAVKYLQAVFAIKDAISKRESREISALFGITVAQVREFFVTQKTRVRKQVRLSREKVVMSNTHALQDDGVPENNNATNHVEPVPLNSIHPEACSISWGEGETVALIPPEDIPPDISDSDKYFVENIFSLLRKEETFSGQVKLMEWIMQIQDASVLIWFLSKGGVLILTTWLSQAASEEQTSVLLLILKVLCHLPLHKASPENMSAILQSVNGLRFYRISDISNRAKGLLSRWTKLFAKIQAMKKQNRNSSQIDSQSQLLLKQSIAEIMGDSSNPEDILSLSNGKSENVRRIESSQGPKLLLTSADDSTKKHMLGSNPSYNKERRKVQMVEQPGQKAAGKSPQTVRIGTSGRSRPMSADDIQKAKMRALYMQSKNSKKDPLPSAIGDSKIVAPEKPLALHSAKDSPPIQNNEAKTEDTPVLSTVQPVNGFSTIQPVNGPSAVQPVNGPLAVQPVNGPSALQPVNGPSAVIVPVQADEIKKPSTPPKSISSKVGVMMKMSSQTILKNCKRKQIDWHVPPGMELDELWRVAAGGNSKEADVQRNRNRRERETTYQSLQTIPLNPKEPWDREMDYDDSLTPEIPSQQPPEESLTEPQDSLDERRIAAGAATTSSSLSSPEPDLELLAALLKNPDLVYALTSGKPSNLAGQDMVKLLDVIKTGAPNSSSSSNKQVEERVEVSLPSPTPSTNPGMSGWGQEGIRNPFSRQNQVGTAVARSGTQLRVGSMQWHQTNEQSIPRHAPSAYSNSITLAHTEREQQQYMQPKLHHNLHFQQQQQQPISTTSYAVREPVGQMGTGTSSSWRSQQSQNSYYSHQENEIASASQVTSYQGNSQYMSSNPGYESWSPDNSPSRNQLNMRGQQQQASRKHDSSTHPYWNQNKRWR 0DBFF4556190BA77 suf4_arath Protein SUPPRESSOR OF FRI 4 SUF4 F17F8.14 At1g30970 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGKKKKRATEKVWCYYCDREFDDEKILVQHQKAKHFKCHVCHKKLSTASGMVIHVLQVHKENVTKVPNAKDGRDSTDIEIYGMQGIPPHVLTAHYGEEEDEPPAKVAKVEIPSAPLGGVVPRPYGMVYPPQQVPGAVPARPMYYPGPPMRHPAPVWQMPPPRPQQWYPQNPALSVPPAAHLGYRPQPLFPVQNMGMTPTPTSAPAIQPSPVTGVTPPGIPTSSPAMPVPQPLFPVVNNSIPSQAPPFSAPLPVGGAQQPSHADALGSADAYPPNNSIPGGTNAHSYASGPNTSGPSIGPPPVIANKAPSNQPNEVYLVWDDEAMSMEERRMSLPKYKVHDETSQMNSINAAIDRRISESRLAGRMAF B9B2356EC3D063EE frigi_arath Protein FRIGIDA F6N23.25 FRI At4g00650 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSNYPPTVAAQPTTTANPLLQRHQSEQRRRELPKIVETESTSMDITIGQSKQPQFLKSIDELAAFSVAVETFKRQFDDLQKHIESIENAIDSKLESNGVVLAARNNNFHQPMLSPPRNNVSVETTVTVSQPSQEIVPETSNKPEGGRMCELMCSKGLRKYIYANISDQAKLMEEIPSALKLAKEPAKFVLDCIGKFYLQGRRAFTKESPMSSARQVSLLILESFLLMPDRGKGKVKIESWIKDEAETAAVAWRKRLMTEGGLAAAEKMDARGLLLLVACFGVPSNFRSTDLLDLIRMSGSNEIAGALKRSQFLVPMVSGIVESSIKRGMHIEALEMVYTFGMEDKFSAALVLTSFLKMSKESFERAKRKAQSPLAFKEAATKQLAVLSSVMQCMETHKLDPAKELPGWQIKEQIVSLEKDTLQLDKEMEEKARSLSLMEEAALAKRMYNQQIKRPRLSPMEMPPVTSSSYSPIYRDRSFPSQRDDDQDEISALVSSYLGPSTSFPHRSRRSPEYMVPLPHGGLGRSVYAYEHLAPNSYSPGHGHRLHRQYSPSLVHGQRHPLQYSPPIHGQQQLPYGIQRVYRHSPSEERYLGLSNQRSPRSNSSLDPK FE8572DFF3E6726F suf4 1 7 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Figure 6A SUF4 and FRI showed homodimerization activity suf4-fr1 1 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association Figure 6A However, the interaction analysis showed that SUF4 binds to both FRI and FRL1 fr1-suf4 1 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Figure 6A However, the interaction analysis showed that SUF4 binds to both FRI and FRL1 fr1-1 1 8 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Figure 6A In addition, SUF4 and FRI showed homodimerization activity ld-suf4 1 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Figure 6A LD protein bound to SUF4 but not to FRI or FRL1 in yeast two-hybrid analysis ld-2 1 6 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Figure 6A frl1-suf4 1 7 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Figure 6A However, the interaction analysis showed that SUF4 binds to both FRI and FRL1 fr1-suf4-1 3 7 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 6C By contrast, SUF4:MYC was detected in the anti-HIS or anti-HA immunoprecipitates repeatedly, demonstrating the direct physical interactions of SUF4 with FRI, FRL1, and LD in plant cells. ld-suf4-1 3 7 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 6C By contrast, SUF4:MYC was detected in the anti-HIS or anti-HA immunoprecipitates repeatedly, demonstrating the direct physical interactions of SUF4 with FRI, FRL1, and LD in plant cells. frl1-suf4-1 3 7 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 6C By contrast, SUF4:MYC was detected in the anti-HIS or anti-HA immunoprecipitates repeatedly, demonstrating the direct physical interactions of SUF4 with FRI, FRL1, and LD in plant cells. suf4-flc_gene 2 7 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey association association Figure 7 SUF4 Binding to the FLC Promoter Region. The authors state that " a region of FLC promoter ∼545 to 850 bp upstream from the transcription initiation site, detected by the FLC-3 primer set, was highly enriched in 35S-SUF4 tagged with MYC epitope" (immunoprecipitated sample). The authors concluded that this " result showed that SUF4 binds to the region around FLC-3 in vivo, suggesting that SUF4 is recruited to the promoter region of FLC for transcriptional activation". suf4-frl1-1 1 7 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association Figure 6A However, the interaction analysis showed that SUF4 binds to both FRI and FRL1 suf4-ld 1 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Figure 6B When the bait and prey were changed, the yeast cells grew slowly but confirmed the interaction of LD and SUF4