IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The role of the Arabidopsis E2FB transcription factor in regulating auxin-dependent cell division. The role of the Arabidopsis E2FB transcription factor in regulating auxin-dependent cell division. Plant Cell (1040-4651) 2005 imex curation Magyar Z., De Veylder L., Atanassova A., Bako L., Inze D., Bogre L. l.bogre@rhul.ac.uk 25-Jun-2008: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Magyar Z., De Veylder L., Atanassova A., Bako L., Inze D., Bogre L. l.bogre@rhul.ac.uk Accepted 2008-JUN-27 by CDEROW. Plant Cell (1040-4651) 2005 Only protein-protein interactions imex curation The role of the Arabidopsis E2FB transcription factor in regulating auxin-dependent cell division. The role of the Arabidopsis E2FB transcription factor in regulating auxin-dependent cell division. Plant Cell (1040-4651) 2005 imex curation Magyar Z., De Veylder L., Atanassova A., Bako L., Inze D., Bogre L. l.bogre@rhul.ac.uk 25-Jun-2008: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Magyar Z., De Veylder L., Atanassova A., Bako L., Inze D., Bogre L. It was not possible to ascertain the accession number of the H1 protein used in the histone H1 kinase assays. Therfore these assays were not curated. l.bogre@rhul.ac.uk Accepted 2008-JUN-27 by CDEROW. 25-Jun-2008: Contacted by JYOTI. Plant Cell (1040-4651) 2005 Only protein-protein interactions imex curation The role of the Arabidopsis E2FB transcription factor in regulating auxin-dependent cell division. The role of the Arabidopsis E2FB transcription factor in regulating auxin-dependent cell division. Plant Cell (1040-4651) 2005 imex curation Magyar Z., De Veylder L., Atanassova A., Bako L., Inze D., Bogre L. l.bogre@rhul.ac.uk 25-Jun-2008: Contacted by JYOTI. Only protein-protein interactions tobac-by_2 Tobacco cell line by_2 Tobacco cell line An undifferentiated cell line derived from tobacco callus tissue. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Magyar Z., De Veylder L., Atanassova A., Bako L., Inze D., Bogre L. l.bogre@rhul.ac.uk Accepted 2008-JUN-27 by CDEROW. 25-Jun-2008: Contacted by JYOTI. Plant Cell (1040-4651) 2005 Only protein-protein interactions imex curation rbr1_arath Retinoblastoma-related protein 1 RBR1 At3g12280 RBR F28J15.11 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEVQPPVTPPIEPNGKRSEASLLDICEKVLSLDGSTCDEALKLFTETKRILSASMSNIGSGTREEVERFWFAFILYSVKRLSVRKEADGLSVSGDNEFNLCQILRALKLNIVDFFKELPQFVVKAGSVLGELYGADWENRLQAKEVQANFVHLSLLSKYYKRGFREFFLTYDANAEKNSANSSTYLLDSYRFGWLLFLALRNHAFSRFKDLVTCSNGVVSILAILIIHVPCRFRNFSIQDSSRFVKKGDKGVDLVASLCKIYDASEDELRIVIDKANNLVETILKKKPSPASECQTDKLDNIDPDGLTYFEDLLEETSISTSLITLEKDYYDGKGELDERVFINEEDSLLGSGSLSAGAVNITGVKRKIDALSSPARTFISPLSPHKSPAAKTNGISGATKLAATPVSTAMTTAKWLRTVISPLLPKPSPGLEHFLKSCDRDITNDVTRRAHIILEAIFPNSSLGAQCGGGSLQAVDLMDDIWAEQRRLEACKLYYRVLEAMCKAEAQILHANNLNSLLTNERFHRCMLACSAELVLATHKTITMLFPAVLERTGITAFDLSKVIESFIRHEDSLPRELRRHLNSLEERLLESMVWEKGSSMYNSLIVARPSLALEINQLGLLAEPMPSLDAIAALINFSDGANHASSVQKHETCPGQNGGIRSPKRLCTDYRSILVERNSFTSPVKDRLLALGNVKSKMLPPPLQSAFASPTRPNPGGGGETCAETGINIFFTKINKLAAVRINGMVERLQLSQQIRESVYCFFQHVLAQRTSLLFSRHIDQIILCCFYGVAKISQMSLTFREIIYNYRKQPQCKPLVFRSVYVDALQCRRQGRIGPDHVDIITFYNEIFIPAVKPLLVELGPVRNDRAVEANNKPEGQCPGSPKVSVFPSVPDMSPKKVSAVHNVYVSPLRGSKMDALISHSTKSYYACVGESTHAYQSPSKDLSAINNRLNNSSSNRKRTLNFDAEAGMVSDSMVANSLNLQNQNQNQNGSDASSSGGAAPLKTEPTDS DIP protein Q9LKZ3 original sequence version: 38 DIP protein Q9LKZ3 original sequence version: 93 F3CD279ED508C263 e2fb_arath Transcription factor E2FB At5g22220 E2FB E2F1 E2F transcription factor-1 T6G21 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSEEVPQQFPSSKRQLHPSLSSMKPPLVAPGEYHRFDAAETRGGGAVADQVVSDAIVIKSTLKRKTDLVNQIVEVNELNTGVLQTPVSGKGGKAKKTSRSAKSNKSGTLASGSNAGSPGNNFAQAGTCRYDSSLGLLTKKFINLIKQAEDGILDLNKAADTLEVQKRRIYDITNVLEGIGLIEKTLKNRIQWKGLDVSKPGETIESIANLQDEVQNLAAEEARLDDQIRESQERLTSLSEDENNKRLLFVTENDIKNLPCFQNKTLIAVKAPHGTTLEVPDPDEAGGYQRRYRIILRSTMGPIDVYLVSQFEESFEDIPQADEPSNVPDEPSNVPDVPSNLPSTSGLPENHDVSMPMKEESTERNMETQEVDDTQRVYSDIESHDFVDGIMKIVPPDLDMGVDYWFRSEVGEVSITDMWPDESGPDWNQMITFDQDHAGPSDNKILEQPQTPSSPTPEESTATRSPTGS ADE1104B34651B7C dpa_arath Transcription factor-like protein DPA DPA DP2A DP-like protein A At5g02470 E2F dimerization partner protein A T22P11.60 DP2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSMEMELFVTPEKQRQHPSVSVEKTPVRRKLIVDDDSEIGSEKKGQSRTSGGGLRQFSVMVCQKLEAKKITTYKEVADEIISDFATIKQNAEKPLNENEYNEKNIRRRVYDALNVFMALDIIARDKKEIRWKGLPITCKKDVEEVKMDRNKVMSSVQKKAAFLKELREKVSSLESLMSRNQEMVVKTQGPAEGFTLPFILLETNPHAVVEIEISEDMQLVHLDFNSTPFSVHDDAYILKLMQEQKQEQNRVSSSSSTHHQSQHSSAHSSSSSCIASGTSGPVCWNSGSIDTR 644324E13561FEC5 dpa-e2f1-2 1 5 unspecified role unspecified role prey prey arath-cell-culture Arabidopsis thaliana cell culture plant_cell_culture plant cellular culture The culture conditions should always be cited in the experimental modifications annotation Either whole plant or undifferentiated plant cells maintained in culture. 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) physical association physical association Figure 1B To prove that the endogenous Arabidopsis E2FB is able to dimerize with DPA as well as to bind RBR1, we conducted pull-down experiments with glutathione S-transferase (GST)-tagged Arabidopsis DPA and RBR1 proteins and Arabidopsis cell extracts. We found that E2FB could associate with both RBR1 and DPA and therefore has the characteristics of the canonical E2F transcriptional complexe rbr1-e2f1 1 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) 5 unspecified role unspecified role prey prey arath-cell-culture Arabidopsis thaliana cell culture plant_cell_culture plant cellular culture The culture conditions should always be cited in the experimental modifications annotation Either whole plant or undifferentiated plant cells maintained in culture. physical association physical association Figure 1B To prove that the endogenous Arabidopsis E2FB is able to dimerize with DPA as well as to bind RBR1, we conducted pull-down experiments with glutathione S-transferase (GST)-tagged Arabidopsis DPA and RBR1 proteins and Arabidopsis cell extracts. We found that E2FB could associate with both RBR1 and DPA and therefore has the characteristics of the canonical E2F transcriptional complexe dpa-e2f1-3 3 6 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 3C The epitope-tagged E2FB and DPA proteins are likely to be functional in BY-2 cells because they form heterodimers (Figure 3C) The tobacco BY-2 cells were transformed by Agrobacterium tumefaciens-mediated transformation. e2f1-dpa-2 3 5 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 3C The epitope-tagged E2FB and DPA proteins are likely to be functional in BY-2 cells because they form heterodimers (Figure 3C) The tobacco BY-2 cells were transformed by Agrobacterium tumefaciens-mediated transformation rbr1-e2f1-dpa 2 5 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined tobac-by_2 Tobacco cell line by_2 Tobacco cell line An undifferentiated cell line derived from tobacco callus tissue. 6 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined tobac-by_2 Tobacco cell line by_2 Tobacco cell line An undifferentiated cell line derived from tobacco callus tissue. 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) association association Figure 3D The epitope-tagged E2FB and DPA proteins are likely to be functional in BY-2 cells because they form heterodimers (Figure 3C) and interact with the bacterially purified Arabidopsis RBR1