IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Autoregulation and homodimerization are involved in the activation of the plant steroid receptor BRI1. Autoregulation and homodimerization are involved in the activation of the plant steroid receptor BRI1. Dev. Cell (1534-5807) 2005 Wang X., Li X., Meisenhelder J., Hunter T., Yoshida S., Asami T., Chory J. chory@salk.edu 16-Oct-2008: Contacted by JYOTI. orchard arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wang X., Li X., Meisenhelder J., Hunter T., Yoshida S., Asami T., Chory J. Transgenic arabidopsis plants expressing epitope tagged proteins of interest were used in this study. chory@salk.edu Accepted 2008-SEP-26 by ORCHARD. 16-Oct-2008: Contacted by JYOTI. Dev. Cell (1534-5807) 2005 orchard Autoregulation and homodimerization are involved in the activation of the plant steroid receptor BRI1. Autoregulation and homodimerization are involved in the activation of the plant steroid receptor BRI1. Dev. Cell (1534-5807) 2005 Wang X., Li X., Meisenhelder J., Hunter T., Yoshida S., Asami T., Chory J. chory@salk.edu 16-Oct-2008: Contacted by JYOTI. orchard in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Wang X., Li X., Meisenhelder J., Hunter T., Yoshida S., Asami T., Chory J. chory@salk.edu Accepted 2008-SEP-26 by ORCHARD. 16-Oct-2008: Contacted by JYOTI. Dev. Cell (1534-5807) 2005 orchard bri1_arath Protein BRASSINOSTEROID INSENSITIVE 1 Brassinosteroid LRR receptor kinase BRI1 F23K16.30 At4g39400 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKTFSSFFLSVTTLFFFSFFSLSFQASPSQSLYREIHQLISFKDVLPDKNLLPDWSSNKNPCTFDGVTCRDDKVTSIDLSSKPLNVGFSAVSSSLLSLTGLESLFLSNSHINGSVSGFKCSASLTSLDLSRNSLSGPVTTLTSLGSCSGLKFLNVSSNTLDFPGKVSGGLKLNSLEVLDLSANSISGANVVGWVLSDGCGELKHLAISGNKISGDVDVSRCVNLEFLDVSSNNFSTGIPFLGDCSALQHLDISGNKLSGDFSRAISTCTELKLLNISSNQFVGPIPPLPLKSLQYLSLAENKFTGEIPDFLSGACDTLTGLDLSGNHFYGAVPPFFGSCSLLESLALSSNNFSGELPMDTLLKMRGLKVLDLSFNEFSGELPESLTNLSASLLTLDLSSNNFSGPILPNLCQNPKNTLQELYLQNNGFTGKIPPTLSNCSELVSLHLSFNYLSGTIPSSLGSLSKLRDLKLWLNMLEGEIPQELMYVKTLETLILDFNDLTGEIPSGLSNCTNLNWISLSNNRLTGEIPKWIGRLENLAILKLSNNSFSGNIPAELGDCRSLIWLDLNTNLFNGTIPAAMFKQSGKIAANFIAGKRYVYIKNDGMKKECHGAGNLLEFQGIRSEQLNRLSTRNPCNITSRVYGGHTSPTFDNNGSMMFLDMSYNMLSGYIPKEIGSMPYLFILNLGHNDISGSIPDEVGDLRGLNILDLSSNKLDGRIPQAMSALTMLTEIDLSNNNLSGPIPEMGQFETFPPAKFLNNPGLCGYPLPRCDPSNADGYAHHQRSHGRRPASLAGSVAMGLLFSFVCIFGLILVGREMRKRRRKKEAELEMYAEGHGNSGDRTANNTNWKLTGVKEALSINLAAFEKPLRKLTFADLLQATNGFHNDSLIGSGGFGDVYKAILKDGSAVAIKKLIHVSGQGDREFMAEMETIGKIKHRNLVPLLGYCKVGDERLLVYEFMKYGSLEDVLHDPKKAGVKLNWSTRRKIAIGSARGLAFLHHNCSPHIIHRDMKSSNVLLDENLEARVSDFGMARLMSAMDTHLSVSTLAGTPGYVPPEYYQSFRCSTKGDVYSYGVVLLELLTGKRPTDSPDFGDNNLVGWVKQHAKLRISDVFDPELMKEDPALEIELLQHLKVAVACLDDRAWRRPTMVQVMAMFKEIQAGSGIDSQSTIRSIEDGGFSTIEMVDMSIKEVPEGKL DIP protein O22476 original sequence version: 104 DIP protein O22476 original sequence version: 120 DIP protein O22476 original sequence version: 146 DIP protein O22476 original sequence version: 130 DIP protein O22476 original sequence version: 123 C7FBA1C21294E600 bri1-3 2 3 putative self putative self putative self putative self n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true BRI1 intracellular domain with a C-terminal deletion (GST-JK) has higher in vitro autophosphorylation activity than the entire BRI1 intracellular domain Figure 3A autophosphorylation of BRL1 protein The deletion of the c-terminal domain 1157-1196 resulted in higher levels of protein autophosphorylation. bri1-5 2 3 enzyme enzyme neutral component neutral component n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 3 enzyme target enzyme target substrate substrate neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range lys911glu experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation BRI1 Phosphorylation Can Be Intermolecular Figure 5B Phosphorylation of BRL1 (K911E) protein bri1-6 1 3 unspecified role unspecified role prey prey region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association BRI1 interacted with itself in plants, both in the presence and absence of ligand, and BL treatment appeared to increase the extent of coimmunoprecipitation, Figure 5G One hour treatment of seedlings with 1 micromolar brassinosteriod prior to assay.