IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Arabidopsis transthyretin-like protein is a potential substrate of BRASSINOSTEROID-INSENSITIVE 1. The Arabidopsis transthyretin-like protein is a potential substrate of BRASSINOSTEROID-INSENSITIVE 1. Plant Cell (1040-4651) 2004 Nam KH., Li J. jian@umich.edu 03-Mar-2009: Contacted by JYOTI. 03-Feb-2009: Contacted by JYOTI. Arabidopsis cDNA library. jyoti yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Nam KH., Li J. jian@umich.edu Accepted 2008-Not a month!-12 by JYOTI. 03-Mar-2009: Contacted by JYOTI. Arabidopsis cDNA library. Plant Cell (1040-4651) 2004 jyoti The Arabidopsis transthyretin-like protein is a potential substrate of BRASSINOSTEROID-INSENSITIVE 1. The Arabidopsis transthyretin-like protein is a potential substrate of BRASSINOSTEROID-INSENSITIVE 1. Plant Cell (1040-4651) 2004 Nam KH., Li J. jian@umich.edu 03-Mar-2009: Contacted by JYOTI. 03-Feb-2009: Contacted by JYOTI. Arabidopsis cDNA library. jyoti yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Nam KH., Li J. jian@umich.edu Accepted 2008-Not a month!-12 by JYOTI. 03-Mar-2009: Contacted by JYOTI. Arabidopsis cDNA library. Plant Cell (1040-4651) 2004 jyoti The Arabidopsis transthyretin-like protein is a potential substrate of BRASSINOSTEROID-INSENSITIVE 1. The Arabidopsis transthyretin-like protein is a potential substrate of BRASSINOSTEROID-INSENSITIVE 1. Plant Cell (1040-4651) 2004 Nam KH., Li J. jian@umich.edu 03-Mar-2009: Contacted by JYOTI. 03-Feb-2009: Contacted by JYOTI. Arabidopsis cDNA library. jyoti in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Nam KH., Li J. jian@umich.edu Accepted 2008-Not a month!-12 by JYOTI. 03-Mar-2009: Contacted by JYOTI. Arabidopsis cDNA library. Plant Cell (1040-4651) 2004 jyoti bri1_arath Protein BRASSINOSTEROID INSENSITIVE 1 Brassinosteroid LRR receptor kinase BRI1 F23K16.30 At4g39400 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKTFSSFFLSVTTLFFFSFFSLSFQASPSQSLYREIHQLISFKDVLPDKNLLPDWSSNKNPCTFDGVTCRDDKVTSIDLSSKPLNVGFSAVSSSLLSLTGLESLFLSNSHINGSVSGFKCSASLTSLDLSRNSLSGPVTTLTSLGSCSGLKFLNVSSNTLDFPGKVSGGLKLNSLEVLDLSANSISGANVVGWVLSDGCGELKHLAISGNKISGDVDVSRCVNLEFLDVSSNNFSTGIPFLGDCSALQHLDISGNKLSGDFSRAISTCTELKLLNISSNQFVGPIPPLPLKSLQYLSLAENKFTGEIPDFLSGACDTLTGLDLSGNHFYGAVPPFFGSCSLLESLALSSNNFSGELPMDTLLKMRGLKVLDLSFNEFSGELPESLTNLSASLLTLDLSSNNFSGPILPNLCQNPKNTLQELYLQNNGFTGKIPPTLSNCSELVSLHLSFNYLSGTIPSSLGSLSKLRDLKLWLNMLEGEIPQELMYVKTLETLILDFNDLTGEIPSGLSNCTNLNWISLSNNRLTGEIPKWIGRLENLAILKLSNNSFSGNIPAELGDCRSLIWLDLNTNLFNGTIPAAMFKQSGKIAANFIAGKRYVYIKNDGMKKECHGAGNLLEFQGIRSEQLNRLSTRNPCNITSRVYGGHTSPTFDNNGSMMFLDMSYNMLSGYIPKEIGSMPYLFILNLGHNDISGSIPDEVGDLRGLNILDLSSNKLDGRIPQAMSALTMLTEIDLSNNNLSGPIPEMGQFETFPPAKFLNNPGLCGYPLPRCDPSNADGYAHHQRSHGRRPASLAGSVAMGLLFSFVCIFGLILVGREMRKRRRKKEAELEMYAEGHGNSGDRTANNTNWKLTGVKEALSINLAAFEKPLRKLTFADLLQATNGFHNDSLIGSGGFGDVYKAILKDGSAVAIKKLIHVSGQGDREFMAEMETIGKIKHRNLVPLLGYCKVGDERLLVYEFMKYGSLEDVLHDPKKAGVKLNWSTRRKIAIGSARGLAFLHHNCSPHIIHRDMKSSNVLLDENLEARVSDFGMARLMSAMDTHLSVSTLAGTPGYVPPEYYQSFRCSTKGDVYSYGVVLLELLTGKRPTDSPDFGDNNLVGWVKQHAKLRISDVFDPELMKEDPALEIELLQHLKVAVACLDDRAWRRPTMVQVMAMFKEIQAGSGIDSQSTIRSIEDGGFSTIEMVDMSIKEVPEGKL DIP protein O22476 original sequence version: 104 DIP protein O22476 original sequence version: 120 DIP protein O22476 original sequence version: 146 DIP protein O22476 original sequence version: 130 DIP protein O22476 original sequence version: 123 C7FBA1C21294E600 tthl_arath Uric acid degradation bifunctional protein TTL TTL Transthyretin-like protein MCK7.9 At5g58220 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAMEIGEDEWKVCCGSSEFAKQMSTSGPLTSQEAIYTARDIWFNQVNVTDWLEAFSAHPQIGNTPSPSINSDFARRSVSEQSTAFATTSASALQELAEWNVLYKKKFGFIFIICASGRTHAEMLHALKERYENRPIVELEIAAMEQMKITELRMAKLFSDKAKVISETDSSSSPVSTKPQDRLRIIGGHLNVAAEAKAPKRSRPPITTHVLDVSRGAPAAGVEVHLEVWSGTTGPSFVHGGGGVWSSVGTSATDRDGRSGPLMDLVDALNPGTYRISFDTAKYSPGCFFPYVSIVFQVTESQKWEHFHVPLLLAPFSFSTYRGS 99191F450BDE262F brl1-ttl 1 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range 5 unspecified role unspecified role prey prey physical association physical association To identify potential targets of the activated BRI1 receptor kinase in response to BR signal, we conducted a yeast two-hybrid screening using the kinase domain of BRI1 (BRI1CK) as the bait. Out of 96 positive BRI1-interacting clones, 60 were found to be derived from the same Arabidopsis gene (At5g58220) that encodes a hypothetical protein of 324 amino acids. The range of the kinase domain used in the yeast two hybrid assay was not described in the text. The range is taken from Li and Chroy (1997) , PMID PMID: 9298904. Because the range is not directly taken from the source text, it is presented as a fuzzy range to indicate that the range was not explicitly stated by the authors of the paper under curation. brl1-ttl-1 2 5 unspecified role unspecified role prey prey n-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role bait bait regio sufficient to bind sufficient binding region sufficient to bind range range range range O22476:p.Gly1048Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain G D Wed May 02 17:30:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly1048asp' Tue Jun 30 20:13:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly1048Asp' O22476:p.Glu1078Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain E K Wed May 02 20:27:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1078lys' Tue Jun 30 16:42:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu1078Lys' physical association physical association The N Terminus of TTL Is Crucial for TTL�BRI1 Interaction The range of the kinase domain used in the yeast two hybrid assay was not described in the text. The range is taken from Li and Chroy (1997) , PMID PMID: 9298904. Because the range is not directly taken from the source text, it is presented as a fuzzy range to indicate that the range was not explicitly stated by the authors of the paper under curation. Table 1 Figure 2B It should be noted that when the n-terminal 29 amino acid region of the TTL protein, demonstrated to be critical for interaction with BRL1, was fused to a protein which does not usually interact with BRL1; no interaction occurred. The authors concluded that other regions, besides the n-termianl region of the TTL protein, facilitated interaction with BRL1. brl1-4 3 4 putative self putative self putative self putative self O22476:p.Gly1048Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain G D Wed May 02 14:51:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly1048asp' Tue Jun 30 17:38:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly1048Asp' region sufficient to bind sufficient binding region sufficient to bind range range range range O22476:p.Glu1078Lys mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain E K Wed May 02 19:00:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1078lys' Tue Jun 30 15:47:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu1078Lys' O22476:p.Ala1031Thr mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain A T Wed May 02 18:48:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala1031thr' Tue Jun 30 16:48:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala1031Thr' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true As shown in Figure 2C, the wild-type GST-BRI1CK fusion protein exhibited strong autophosphorylation activity in vitro, whereas no autophosphorylation was detected for the MBP-TTL fusion protein. The range of the kinase domain used in the yeast two hybrid assay was not described in the text. The range is taken from Li and Chroy (1997) , PMID PMID: 9298904. Because the range is not directly taken from the source text, it is presented as a fuzzy range to indicate that the range was not explicitly stated by the authors of the paper under curation. Figure 2C autophosphorylation of BRL1 protein brl1-tll 3 5 enzyme target enzyme target substrate substrate neutral component neutral component region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 enzyme enzyme neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O22476:p.Glu1078Lys mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain E K Wed May 02 17:51:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1078lys' Tue Jun 30 15:44:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu1078Lys' O22476:p.Gly1048Asp mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain G D Wed May 02 17:26:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly1048asp' Tue Jun 30 20:21:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly1048Asp' O22476:p.Ala1031Thr mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain A T Wed May 02 19:45:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala1031thr' Tue Jun 30 18:26:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala1031Thr' region sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation However, TTL was phosphorylated when the two fusion proteins were mixed together in the kinase assay solution. The range of the kinase domain used in the yeast two hybrid assay was not described in the text. The range is taken from Li and Chroy (1997) , PMID PMID: 9298904. Because the range is not directly taken from the source text, it is presented as a fuzzy range to indicate that the range was not explicitly stated by the authors of the paper under curation. Figure 2C phosphorylation of TLL protein autophosphorylation of BRL1 protein