IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Interaction specificity of Arabidopsis calcineurin B-like calcium sensors and their target kinases. Interaction specificity of Arabidopsis calcineurin B-like calcium sensors and their target kinases. Plant Physiol. (0032-0889) 2000 imex curation Kim KN., Cheong YH., Gupta R., Luan S. sluan@nature.berkeley.edu 03-Feb-2009: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kim KN., Cheong YH., Gupta R., Luan S. sluan@nature.berkeley.edu Accepted 2008-Not a month!-15 by ORCHARD. 03-Mar-2009: Contacted by JYOTI. Plant Physiol. (0032-0889) 2000 orchard Only protein-protein interactions imex curation cipk3_arath CBL-interacting serine/threonine-protein kinase 3 CIPK3 PKS12 SnRK3.17 SOS2-like protein kinase PKS12 SNF1-related kinase 3.17 T20P8.3 At2g26980 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MNRRQQVKRRVGKYEVGRTIGEGTFAKVKFARNSETGEPVALKILDKEKVLKHKMAEQIRREIATMKLIKHPNVVQLYEVMASKTKIFIILEYVTGGELFDKIVNDGRMKEDEARRYFQQLIHAVDYCHSRGVYHRDLKPENLLLDSYGNLKISDFGLSALSQQVRDDGLLHTSCGTPNYVAPEVLNDRGYDGATADMWSCGVVLYVLLAGYLPFDDSNLMNLYKKISSGEFNCPPWLSLGAMKLITRILDPNPMTRVTPQEVFEDEWFKKDYKPPVFEERDDSNMDDIDAVFKDSEEHLVTEKREEQPAAINAFEIISMSRGLNLENLFDPEQEFKRETRITLRGGANEIIEKIEEAAKPLGFDVQKKNYKMRLENVKAGRKGNLNVATEIFQVAPSLHMVQVSKSKGDTLEFHKFYKKLSNSLEQVVWTNNEVKKETAK 1B9D9CB78E178138 cipk6_arath CBL-interacting serine/threonine-protein kinase 6 SOS2-like protein kinase PKS4 SNF1-related kinase 3.14 SOS3-interacting protein 3 CIPK6 PKS4 At4g30960 SIP3 SnRK3.14 F6I18.130 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVGAKPVENGSDGGSSTGLLHGRYELGRLLGHGTFAKVYHARNIQTGKSVAMKVVGKEKVVKVGMVDQIKREISVMRMVKHPNIVELHEVMASKSKIYFAMELVRGGELFAKVAKGRLREDVARVYFQQLISAVDFCHSRGVYHRDLKPENLLLDEEGNLKVTDFGLSAFTEHLKQDGLLHTTCGTPAYVAPEVILKKGYDGAKADLWSCGVILFVLLAGYLPFQDDNLVNMYRKIYRGDFKCPGWLSSDARRLVTKLLDPNPNTRITIEKVMDSPWFKKQATRSRNEPVAATITTTEEDVDFLVHKSKEETETLNAFHIIALSEGFDLSPLFEEKKKEEKREMRFATSRPASSVISSLEEAARVGNKFDVRKSESRVRIEGKQNGRKGKLAVEAEIFAVAPSFVVVEVKKDHGDTLEYNNFCSTALRPALKDIFWTSTPA 469728C6CB83F428 cipk1_arath CBL-interacting serine/threonine-protein kinase 1 CIPK1 PKS13 SnRK3.16 MKP6.6 At3g17510 SOS2-like protein kinase PKS13 SNF1-related kinase 3.16 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVRRQEEEKKAEKGMRLGKYELGRTLGEGNFGKVKFAKDTVSGHSFAVKIIDKSRIADLNFSLQIKREIRTLKMLKHPHIVRLHEVLASKTKINMVMELVTGGELFDRIVSNGKLTETDGRKMFQQLIDGISYCHSKGVFHRDLKLENVLLDAKGHIKITDFGLSALPQHFRDDGLLHTTCGSPNYVAPEVLANRGYDGAASDIWSCGVILYVILTGCLPFDDRNLAVLYQKICKGDPPIPRWLSPGARTMIKRMLDPNPVTRITVVGIKASEWFKLEYIPSIPDDDDEEEVDTDDDAFSIQELGSEEGKGSDSPTIINAFQLIGMSSFLDLSGFFEQENVSERRIRFTSNSSAKDLLEKIETAVTEMGFSVQKKHAKLRVKQEERNQKGQVGLSVTAEVFEIKPSLNVVELRKSYGDSCLYRQLYERLLKDVGTSSPEQEIVT 63DB8AF69CCAA5C9 cnbl1_arath Calcineurin B-like protein 1 SOS3-like calcium-binding protein 5 CBL1 SCABP5 At4g17615 dl4845w FCAALL.122 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGCFHSKAAKEFRGHEDPVKLASETAFSVSEVEALFELFKSISSSVVDDGLINKEEFQLALFKSRKRENIFANRIFDMFDVKRKGVIDFGDFVRSLNVFHPNASLEDKIDFTFRLYDMDCTGYIERQEVKQMLIALLCESEMKLADETIEIILDKTFEDADVNQDGKIDKLEWSDFVNKNPSLLKIMTLPYLRDITTTFPSFVFHSEVDEIAT 1C310FC449D027B1 cnbl4_arath Calcineurin B-like protein 4 Protein SALT OVERLY SENSITIVE 3 CBL4 SOS3 At5g24270 MOP9.8 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGCSVSKKKKKNAMRPPGYEDPELLASVTPFTVEEVEALYELFKKLSSSIIDDGLIHKEEFQLALFRNRNRRNLFADRIFDVFDVKRNGVIEFGEFVRSLGVFHPSAPVHEKVKFAFKLYDLRQTGFIEREELKEMVVALLHESELVLSEDMIEVMVDKAFVQADRKNDGKIDIDEWKDFVSLNPSLIKNMTLPYLKDINRTFPSFVSSCEEEEMELQNVSS DIP protein O81223 original sequence version: 98 DF06C3973748AFF7 cnbl3_arath Calcineurin B-like protein 3 SOS3-like calcium-binding protein 6 CBL3 SCABP6 At4g26570 T15N24.20 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSQCIDGFKHVCSSFFRCFDIDIYKQSGGLGDPELLARETVFSVSEIEALYELFKKISSAVIDDGLINKEEFQLALFKTNKKESLFADRVFDLFDTKHNGILGFEEFARALSVFHPNAPIEDKIDFSFQLYDLKQQGFIERQEVKQMVVATLAESGMNLSDEIIESIIDKTFEEADTKHDGRIDKEEWRTLVLRHPSLLKNMTLQYLKDITTTFPSFVFHSQVEDT F78222E98F64983E cipk5_arath CBL-interacting serine/threonine-protein kinase 5 CIPK5 PKS19 SnRK3.24 SOS2-like protein kinase PKS19 SNF1-related kinase 3.24 T30N20.200 At5g10930 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEERRVLFGKYEMGRLLGKGTFAKVYYGKEIIGGECVAIKVINKDQVMKRPGMMEQIKREISIMKLVRHPNIVELKEVMATKTKIFFVMEFVKGGELFCKISKGKLHEDAARRYFQQLISAVDYCHSRGVSHRDLKPENLLLDENGDLKISDFGLSALPEQILQDGLLHTQCGTPAYVAPEVLKKKGYDGAKADIWSCGVVLYVLLAGCLPFQDENLMNMYRKIFRADFEFPPWFSPEARRLISKLLVVDPDRRISIPAIMRTPWLRKNFTPPLAFKIDEPICSQSSKNNEEEEEDGDCENQTEPISPKFFNAFEFISSMSSGFDLSSLFESKRKVQSVFTSRSSATEVMEKIETVTKEMNMKVKRTKDFKVKMEGKTEGRKGRLSMTAEVFEVAPEISVVEFCKSAGDTLEYDRLYEEEVRPALNDIVWSWHGDNNNTSSEDC ECA735B820FCFF0C cipk2_arath CBL-interacting serine/threonine-protein kinase 2 CIPK2 At5g07070 PKS16 SnRK3.2 SOS2-like protein kinase PKS16 SNF1-related kinase 3.2 T28J14.10 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MENKPSVLTERYEVGRLLGQGTFAKVYFGRSNHTNESVAIKMIDKDKVMRVGLSQQIKREISVMRIAKHPNVVELYEVMATKSRIYFVIEYCKGGELFNKVAKGKLKEDVAWKYFYQLISAVDFCHSRGVYHRDIKPENLLLDDNDNLKVSDFGLSALADCKRQDGLLHTTCGTPAYVAPEVINRKGYEGTKADIWSCGVVLFVLLAGYLPFHDTNLMEMYRKIGKADFKCPSWFAPEVKRLLCKMLDPNHETRITIAKIKESSWFRKGLHLKQKKMEKMEKQQVREATNPMEAGGSGQNENGENHEPPRLATLNAFDIIALSTGFGLAGLFGDVYDKRESRFASQKPASEIISKLVEVAKCLKLKIRKQGAGLFKLERVKEGKNGILTMDAEIFQVTPTFHLVEVKKCNGDTMEYQKLVEEDLRPALADIVWVWQGEKEKEEQLLQDEQGEQEPS 948E0CC1735C2555 cbl1-cipk1-3 1 4 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind range range range range 5 unspecified role unspecified role bait bait physical association physical association Table 1 The authors do not provide the amino acid coordinates of the c-terminal region used in this assay. The authors state that "truncated versions lacking the N-terminal kinase domain" were used. The prey protein is therefore annotated with fuzzy coordinates, ( indicating the coordinates are not explicitly stated in the text), which exclude the kinase domain. Coordinates were taken from the UniProt record. cbl3-cipk1-1 1 7 unspecified role unspecified role bait bait c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind range range range range physical association physical association Table 1 The authors do not provide the amino acid coordinates of the c-terminal region used in this assay. The authors state that "truncated versions lacking the N-terminal kinase domain" were used. The prey protein is therefore annotated with fuzzy coordinates, ( indicating the coordinates are not explicitly stated in the text), which exclude the kinase domain. Coordinates were taken from the UniProt record. cbl3-cipk2 1 9 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind range range range range 7 unspecified role unspecified role bait bait c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table 1, Fig 5B The authors do not provide the amino acid coordinates of the c-terminal region used in this assay. The authors state that "truncated versions lacking the N-terminal kinase domain" were used. The prey protein is therefore annotated with fuzzy coordinates, ( indicating the coordinates are not explicitly stated in the text), which exclude the kinase domain. Coordinates were taken from the UniProt record. cbl3-cipk3 1 2 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind range range range range 7 unspecified role unspecified role bait bait n-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table 1 Figure 5B The authors do not provide the amino acid coordinates of the c-terminal region used in this assay. The authors state that "truncated versions lacking the N-terminal kinase domain" were used. The prey protein is therefore annotated with fuzzy coordinates, ( indicating the coordinates are not explicitly stated in the text), which exclude the kinase domain. Coordinates were taken from the UniProt record. cbl1-cipk5 1 8 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role bait bait physical association physical association Table 1 Figure 3 The authors demonstrated that a region of CIPK5 between amino acid residues 197-252 inhibited interaction with CBL proteins. cbl3-cipk5 1 8 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind range range range range 7 unspecified role unspecified role bait bait n-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table 1 The authors demonstrated that a region of CIPK5 between amino acid residues 197-252 inhibited interaction with CBL proteins. cbl4-cipk5-1 1 8 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait physical association physical association Table 1 Figure 3 Figure 4 As shown in Figure 4A, both 35 and 20 amino acid fragments interacted with AtCBL4 but not with AtCBL1 and AtCBL3. Compared with the interaction strength between AtCBL4 and the entire C-terminal region of CIPK5 (CIPK5C), the two fragments interacted with AtCBL4 at significantly reduced levels (Fig. 4B). These results suggest that the conserved domain in the C-terminal region of CIPKs may be sufficient for interacting with certain AtCBL member(s) but may need surrounding sequences to have an optimum interaction. The authors concluded "the conserved domain in the C-terminal region of CIPKs may be sufficient for interacting with certain AtCBL member(s) but may need surrounding sequences to have an optimum interaction". cbl4-cipk6 1 3 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind range range range range 6 unspecified role unspecified role bait bait physical association physical association Table 1 The authors do not provide the amino acid coordinates of the c-terminal region used in this assay. The authors state that "truncated versions lacking the N-terminal kinase domain" were used. The prey protein is therefore annotated with fuzzy coordinates, ( indicating the coordinates are not explicitly stated in the text), which exclude the kinase domain. Coordinates were taken from the UniProt record. cbl3-cipk6 1 7 unspecified role unspecified role bait bait c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind range range range range physical association physical association Table 1 Figure 5 The authors do not provide the amino acid coordinates of the c-terminal region used in this assay. The authors state that "truncated versions lacking the N-terminal kinase domain" were used. The prey protein is therefore annotated with fuzzy coordinates, ( indicating the coordinates are not explicitly stated in the text), which exclude the kinase domain. Coordinates were taken from the UniProt record. cbl1-cipk6 1 5 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind range range range range physical association physical association Table 1 The authors do not provide the amino acid coordinates of the c-terminal region used in this assay. The authors state that "truncated versions lacking the N-terminal kinase domain" were used. The prey protein is therefore annotated with fuzzy coordinates, ( indicating the coordinates are not explicitly stated in the text), which exclude the kinase domain. Coordinates were taken from the UniProt record. cbl1-cipk3 1 2 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind range range range range 5 unspecified role unspecified role bait bait physical association physical association Table 1 The authors do not provide the amino acid coordinates of the c-terminal region used in this assay. The authors state that "truncated versions lacking the N-terminal kinase domain" were used. The prey protein is therefore annotated with fuzzy coordinates, ( indicating the coordinates are not explicitly stated in the text), which exclude the kinase domain. Coordinates were taken from the UniProt record. cbl1-cipk2 1 9 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind range range range range 5 unspecified role unspecified role bait bait physical association physical association Table 1 The authors do not provide the amino acid coordinates of the c-terminal region used in this assay. The authors state that "truncated versions lacking the N-terminal kinase domain" were used. The prey protein is therefore annotated with fuzzy coordinates, ( indicating the coordinates are not explicitly stated in the text), which exclude the kinase domain. Coordinates were taken from the UniProt record. cbl4-cipk2-1 1 6 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind range range range range physical association physical association Table 1 The authors do not provide the amino acid coordinates of the c-terminal region used in this assay. The authors state that "truncated versions lacking the N-terminal kinase domain" were used. The prey protein is therefore annotated with fuzzy coordinates, ( indicating the coordinates are not explicitly stated in the text), which exclude the kinase domain. Coordinates were taken from the UniProt record. cbl4-cipk1-2 1 6 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind range range range range physical association physical association Table 1 The authors do not provide the amino acid coordinates of the c-terminal region used in this assay. The authors state that "truncated versions lacking the N-terminal kinase domain" were used. The prey protein is therefore annotated with fuzzy coordinates, ( indicating the coordinates are not explicitly stated in the text), which exclude the kinase domain. Coordinates were taken from the UniProt record. Interaction could be judged as extremely weak as the growth of the co-transformed Y190 yeast cells was small.