IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Guard cell inward K+ channel activity in arabidopsis involves expression of the twin channel subunits KAT1 and KAT2. Guard cell inward K+ channel activity in arabidopsis involves expression of the twin channel subunits KAT1 and KAT2. J. Biol. Chem. (0021-9258) 2001 imex curation Pilot G., Lacombe B., Gaymard F., Cherel I., Boucherez J., Thibaud JB., Sentenac H. sentenac@ensam.inra.fr 03-Feb-2009: Contacted by JYOTI. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Pilot G., Lacombe B., Gaymard F., Cherel I., Boucherez J., Thibaud JB., Sentenac H. sentenac@ensam.inra.fr Accepted 2008-Not a month!-12 by JYOTI. 03-Mar-2009: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2001 jyoti Only protein-protein interactions imex curation kat2_arath Potassium channel KAT2 KAT2 T9A21.140 At4g18290 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSISCTRNFFKRFCVEEYNMDTFKHSSFLSADLLPSLGARINQSTKLRKHIISPFDPRFRGWEMWLVILVIYSAWICPFEFAFITYKKDALFIIDNIVNGFFAIDIILTFFVAYLDSHSYLLVDKPKKIAIRYLSTWFAFDVCSTAPFQSLSLLFKYNGSEIGFRVLSMLRLWRLRRVSSLFARLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFAYLIADQYHDPTKTWIGAVYPNFKETSVWSRYVTALYWSITTLTTTGYGDLHAENPREMLFFVFFMLFNLGFTSYLIGNMTNLVVHWTSRTRNFRDTVRAASEFASRNQLPPNIQDQMLSHICLKFKTEGLKQQEALNGLPKAIRSSIANYLFFPIVQNVYLFHGVSRNFLFQLVSDIDAEYFPPREDVILQNEAPTDLYILVSGAVDFTVYVGEEDQVQGKAVVGDAFGEIGVLCYTPQPFTVRTTELSQILRISKKSLMSAMRAHVEDGRVIMNNLFMKLRGQQSIAIDDPNSEPESLLKEWLVGGSKTGEGNASDQGHGHKYLQLHDSENIDMGSTEWRDSRRSGYGETKRVREHTIEIEEGEKPNKEFDGKGCSDADLTSFEFHSQEAYPYCRSNIQIKQHEAAKPKDKRVTIHLKSRDKDLSKLIILPASIEELLRLAGEKFGYSFTKVTNAENAEIDDEDVIRDGDHLYILINENS 55E1DFE6AB2A2453 kat1_arath Potassium channel KAT1 KAT1 MPL12.2 At5g46240 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSISWTRNFFERFCVEEYNIDTIKQSSFLSADLLPSLGARINQSTKLRKHIISPFNPRYRAWEMWLVLLVIYSAWICPFQFAFITYKKDAIFIIDNIVNGFFAIDIILTFFVAYLDSHSYLLVDSPKKIAIRYLSTWFAFDVCSTAPFQPLSLLFNYNGSELGFRILSMLRLWRLRRVSSLFARLEKDIRFNYFWIRCTKLISVTLFAIHCAGCFNYLIADRYPNPRKTWIGAVYPNFKEASLWNRYVTALYWSITTLTTTGYGDFHAENPREMLFDIFFMMFNLGLTAYLIGNMTNLVVHWTSRTRTFRDSVRAASEFASRNQLPHDIQDQMLSHICLKFKTEGLKQQETLNNLPKAIRSSIANYLFFPIVHNIYLFQGVSRNFLFQLVSDIDAEYFPPKEDIILQNEAPTDLYILVSGAVDFTVYVDGHDQFQGKAVIGETFGEVGVLYYRPQPFTVRTTELSQILRISRTSLMSAMHAHADDGRVIMNNLFMKLRGQQSIAIDDSNTSGHENRDFKSMGWEEWRDSRKDGYGLDVTNPTSDTALMDAIHKEDTEMVKKILKEQKIERAKVERSSSETAGRSYANDSSKKDPYCSSSNQIIKPCKREEKRVTIHMMSESKNGKLILLPSSIEELLRLASEKFGGCNFTKITNADNAEIDDLDVIWDGDHLYFSSN 7F9C8285ED702338 kat1-1 1 3 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range range range 3 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association Figure 6B We therefore investigated the possibility of interactions between the C-terminal domains of KAT1 and KAT2 using the two-hybrid system in yeast (38) and obtained positive results The authors do not provide the amino acid coordinates used in the bait and prey constructs. The coordinates used come from the UniProt record and correspond to the KHA domain. This domain was identified by the authors as a C-terminal domain thought to play a role in channel tetramerization. The coordinates are presented as fuzzy coordinates to indicate they may not be exactly as used in the assay (please see the IntAct annotation manual available from the IntAct webstite for more details). kat1-kat2 1 3 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range 2 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association Figure 6B We therefore investigated the possibility of interactions between the C-terminal domains of KAT1 and KAT2 using the two-hybrid system in yeast (38) and obtained positive results The authors do not provide the amino acid coordinates used in the bait and prey constructs. The coordinates used come from the UniProt record and correspond to the KHA domain. This domain was identified by the authors as a C-terminal domain thought to play a role in channel tetramerization. The coordinates are presented as fuzzy coordinates to indicate they may not be exactly as used in the assay (please see the IntAct annotation manual available from the IntAct webstite for more details). kat2-kat1 1 3 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range range range 2 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association Figure 6B We therefore investigated the possibility of interactions between the C-terminal domains of KAT1 and KAT2 using the two-hybrid system in yeast (38) and obtained positive results The authors do not provide the amino acid coordinates used in the bait and prey constructs. The coordinates used come from the UniProt record and correspond to the KHA domain. This domain was identified by the authors as a C-terminal domain thought to play a role in channel tetramerization. The coordinates are presented as fuzzy coordinates to indicate they may not be exactly as used in the assay (please see the IntAct annotation manual available from the IntAct webstite for more details). kat2-1 1 2 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range range range 2 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association Figure 6B We therefore investigated the possibility of interactions between the C-terminal domains of KAT1 and KAT2 using the two-hybrid system in yeast (38) and obtained positive results The authors do not provide the amino acid coordinates used in the bait and prey constructs. The coordinates used come from the UniProt record and correspond to the KHA domain. This domain was identified by the authors as a C-terminal domain thought to play a role in channel tetramerization. The coordinates are presented as fuzzy coordinates to indicate they may not be exactly as used in the assay (please see the IntAct annotation manual available from the IntAct webstite for more details).