IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Genetic analysis of nif regulatory genes by utilizing the yeast two-hybrid system detected formation of a NifL-NifA complex that is implicated in regulated expression of nif genes. Genetic analysis of nif regulatory genes by utilizing the yeast two-hybrid system detected formation of a NifL-NifA complex that is implicated in regulated expression of nif genes. J. Bacteriol. (0021-9193) 1999 imex curation Lei S., Pulakat L., Gavini N. For the controls the biosource of the proteins is not known ngavini@bgnet.bgsu.edu 14-Nov-2008: Contacted by JYOTI. davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lei S., Pulakat L., Gavini N. For the controls the biosource of the proteins is not known ngavini@bgnet.bgsu.edu Accepted 2008-NOV-17 by DAVET. J. Bacteriol. (0021-9193) 1999 davet Only protein-protein interactions imex curation nifl_azovi Nitrogen fixation regulatory protein nifL protein protein azovi Azotobacter vinelandii MTPANPTLSNEPQAPHAESDELLPEIFRQTVEHAPIAISITDLKANILYANRAFRTITGYGSEEVLGKNESILSNGTTPRLVYQALWGRLAQKKPWSGVLVNRRKDKTLYLAELTVAPVLNEAGETIYYLGMHRDTSELHELEQRVNNQRLMIEAVVNAAPAAMVVLDRQHRVMLSNPSFCRLARDLVEDGSSESLVALLRENLAAPFETLENQGSAFSGKEISFDLGGRSPRWLSCHGRAIHIENEQAHVFFAPTEERYLLLTINDISELRQKQQDSRLNALKALMAEEELLEGMRETFNAAIHRLQGPVNLISAAMRMLERRLGDKAGNDPVLSAMREASTAGMEALENLSGSIPVRMAESKMPVNLNQLIREVITLCTDQLLAQGIVVDWQPALRLPWVMGGESSQRSMIKHLVDNAIESMSQNQVSRRELFISTRVENHLVRMEITDSGPGIPPDLVLKVFEPFFSTKPPHRVGRGMGLPVVQEIVAKHAGMVHVDTDYREGCRIVVELPFSAST A40B1E76BD3723CB nifa_azovi Nif-specific regulatory protein nifA protein protein azovi Azotobacter vinelandii MNATIPQRSAKQNPVELYDLQLQALASIARTLSREQQIDELLEQVLAVLHNDLGLLHGLVTISDPEHGALQIGAIHTDSEAVAQACEGVRYRSGEGVIGNVLKHGNSVVLGRISADPRFLDRLALYDLEMPFIAVPIKNPEGNTIGVLAAQPDCRADEHMPARTRFLEIVANLLAQTVRLVVNIEDGREAADERDELRREVRGKYGFENMVVGHTPTMRRVFDQIRRVAKWNSTVLVLGESGTGKELIASAIHYKSPRAHRPFVRLNCAALPETLLESELFGHEKGAFTGAVKQRKGRFEQADGGTLFLDEIGEISPMFQAKLLRVLQEGEFERVGGNQTVRVNVRIVAATNRDLESEVEKGKFREDLYYRLNVMAIRIPPLRERTADIPELAEFLLGKIGRQQGRPLTVTDSAIRLLMSHRWPGNVRELENCLERSAIMSEDGTITRDVVSLTGVDNESPPLAAPLPEVNLADETLDDRERVIAALEQAGWVQAKAARLLGMTPRQIAYRIQTLNIHMRKI 9556A07EE8A7B209 nifa-nifl 1 2 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association table1 and figure 3 Results of the liquid beta -galactosidase assay with ONPG as substrate to demonstrate NifL-NifA interactions nifa-nifl-1 1 3 unspecified role unspecified role prey prey 2 unspecified role unspecified role bait bait physical association physical association table1 and figure 3 Results of the liquid beta -galactosidase assay with ONPG as substrate to demonstrate NifL-NifA interactions