IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins. Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins. Oncogene (0950-9232) 1998 imex curation Zariwala M., Liu J., Xiong Y. yxiong@unc.edu 23-MAY-2007: Contacted by JYOTI. Returned mail. human HaCaT keratinocyte cell cDNA Only protein-protein interactions human-cls Homo sapiens fibroblast cell line (GM03318) cls Human fibroblast obtained from patients with Coffin-Lowry Syndrome (CLS). GM03318 (NIGMS Human Genetic Cell Repository) Catalog ID GM03318 (NIGMS Human Genetic Cell Repository) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zariwala M., Liu J., Xiong Y. yxiong@unc.edu Accepted 2007-JUN-1 by JYOTI. 23-MAY-2007: Contacted by JYOTI. Returned mail. human HaCaT keratinocyte cell cDNA Oncogene (0950-9232) 1998 Only protein-protein interactions imex curation Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins. Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins. Oncogene (0950-9232) 1998 imex curation Zariwala M., Liu J., Xiong Y. yxiong@unc.edu 23-MAY-2007: Contacted by JYOTI. Returned mail. human HaCaT keratinocyte cell cDNA Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Zariwala M., Liu J., Xiong Y. yxiong@unc.edu Accepted 2007-JUN-1 by JYOTI. 23-MAY-2007: Contacted by JYOTI. Returned mail. human HaCaT keratinocyte cell cDNA Oncogene (0950-9232) 1998 Only protein-protein interactions imex curation Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins. Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins. Oncogene (0950-9232) 1998 imex curation Zariwala M., Liu J., Xiong Y. yxiong@unc.edu 23-MAY-2007: Contacted by JYOTI. Returned mail. human HaCaT keratinocyte cell cDNA Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zariwala M., Liu J., Xiong Y. yxiong@unc.edu Accepted 2007-JUN-1 by JYOTI. 23-MAY-2007: Contacted by JYOTI. Returned mail. human HaCaT keratinocyte cell cDNA Oncogene (0950-9232) 1998 Only protein-protein interactions imex curation Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins. Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins. Oncogene (0950-9232) 1998 imex curation Zariwala M., Liu J., Xiong Y. yxiong@unc.edu 23-MAY-2007: Contacted by JYOTI. Returned mail. human HaCaT keratinocyte cell cDNA Only protein-protein interactions human-ct10 Homo sapiens ct10 SV40 transformed human fbroblasts strains CT10 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zariwala M., Liu J., Xiong Y. yxiong@unc.edu Accepted 2007-JUN-1 by JYOTI. 23-MAY-2007: Contacted by JYOTI. Returned mail. human HaCaT keratinocyte cell cDNA Oncogene (0950-9232) 1998 Only protein-protein interactions imex curation Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins. Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins. Oncogene (0950-9232) 1998 imex curation Zariwala M., Liu J., Xiong Y. yxiong@unc.edu 23-MAY-2007: Contacted by JYOTI. Returned mail. human HaCaT keratinocyte cell cDNA Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Zariwala M., Liu J., Xiong Y. yxiong@unc.edu Accepted 2007-JUN-1 by JYOTI. 23-MAY-2007: Contacted by JYOTI. Returned mail. human HaCaT keratinocyte cell cDNA Oncogene (0950-9232) 1998 Only protein-protein interactions imex curation cks1_human Cyclin-dependent kinases regulatory subunit 1 PNAS-16 PNAS-143 CKS1B CKS1 protein protein human Homo sapiens Human MSHKQIYYSDKYDDEEFEYRHVMLPKDIAKLVPKTHLMSESEWRNLGVQQSQGWVHYMIHEPEPHILLFRRPLPKKPKK E64207B140FA38D5 ccne2_human G1/S-specific cyclin-E2 CCNE2 protein protein human Homo sapiens Human MSRRSSRLQAKQQPQPSQTESPQEAQIIQAKKRKTTQDVKKRREEVTKKHQYEIRNCWPPVLSGGISPCIIIETPHKEIGTSDFSRFTNYRFKNLFINPSPLPDLSWGCSKEVWLNMLKKESRYVHDKHFEVLHSDLEPQMRSILLDWLLEVCEVYTLHRETFYLAQDFFDRFMLTQKDINKNMLQLIGITSLFIASKLEEIYAPKLQEFAYVTDGACSEEDILRMELIILKALKWELCPVTIISWLNLFLQVDALKDAPKVLLPQYSQETFIQIAQLLDLCILAIDSLEFQYRILTAAALCHFTSIEVVKKASGLEWDSISECVDWMVPFVNVVKSTSPVKLKTFKKIPMEDRHNIQTHTNYLAMLEEVNYINTFRKGGQLSPVCNGGIMTPPKSTEKPPGKH DIP protein O96020 original sequence version: 126 D7DC9BEEF3FD62EC cdn1b_human Cyclin-dependent kinase inhibitor 1B Cyclin-dependent kinase inhibitor p27 p27Kip1 CDKN1B KIP1 protein protein human Homo sapiens Human MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDVSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT DIP protein P46527 original sequence version: 120 DIP protein P46527 original sequence version: 115 DIP protein P46527 original sequence version: 154 DIP protein P46527 original sequence version: 168 DIP protein P46527 original sequence version: 164 DIP protein P46527 original sequence version: 118 1118D58901CDF3FC ccnh_human Cyclin-H MO15-associated protein p37 p34 CCNH protein protein human Homo sapiens Human MYHNSSQKRHWTFSSEEQLARLRADANRKFRCKAVANGKVLPNDPVFLEPHEEMTLCKYYEKRLLEFCSVFKPAMPRSVVGTACMYFKRFYLNNSVMEYHPRIIMLTCAFLACKVDEFNVSSPQFVGNLRESPLGQEKALEQILEYELLLIQQLNFHLIVHNPYRPFEGFLIDLKTRYPILENPEILRKTADDFLNRIALTDAYLLYTPSQIALTAILSSASRAGITMESYLSESLMLKENRTCLSQLLDIMKSMRNLVKKYEPPRSEEVAVLKQKLERCHSAELALNVITKKRKGYEDDDYVSKKSKHEEEEWTDDDLVESL DIP protein P51946 original sequence version: 160 DIP protein P51946 original sequence version: 113 BB48D55DA397A0E4 cdk2_human Cyclin-dependent kinase 2 CDK2 p33 protein kinase Cell division protein kinase 2 CDKN2 protein protein human Homo sapiens Human MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL DIP protein P24941 original sequence version: 142 DIP protein P24941 original sequence version: 118 DIP protein P24941 original sequence version: 143 DIP protein P24941 original sequence version: 144 DIP protein P24941 original sequence version: 152 DIP protein P24941 original sequence version: 182 DIP protein P24941-1 original sequence version: 204 DIP protein P24941 original sequence version: 90 DIP protein P24941 original sequence version: 190 DIP protein P24941 original sequence version: 168 F90A0F4E70910B51 ccnd1_human G1/S-specific cyclin-D1 CCND1 BCL1 PRAD1 PRAD1 oncogene BCL-1 oncogene B-cell lymphoma 1 protein protein protein human Homo sapiens Human MEHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSMRKIVATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLTAEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI DIP protein P24385 original sequence version: 163 DIP protein P24385 original sequence version: 165 DIP protein P24385 original sequence version: 160 DIP protein P24385 original sequence version: 121 DIP protein P24385 original sequence version: 122 DIP protein P24385 original sequence version: 143 DIP protein P24385 original sequence version: 127 3CC00C9905F58D3A cdkn3_human Cyclin-dependent kinase inhibitor 3 CDKN3 CDI1 CIP2 KAP CDK2-associated dual-specificity phosphatase Kinase-associated phosphatase Cyclin-dependent kinase-interacting protein 2 Cyclin-dependent kinase interactor 1 protein protein human Homo sapiens Human MKPPSSIQTSEFDSSDEEPIEDEQTPIHISWLSLSRVNCSQFLGLCALPGCKFKDVRRNVQKDTEELKSCGIQDIFVFCTRGELSKYRVPNLLDLYQQCGIITHHHPIADGGTPDIASCCEIMEELTTCLKNYRKTLIHCYGGLGRSCLVAACLLLYLSDTISPEQAIDSLRDLRGSGAIQTIKQYNYLHEFRDKLAAHLSSRDSQSRSVSR DIP protein Q16667 original sequence version: 104 D87FAC8E28F6525F cdn1c_human Cyclin-dependent kinase inhibitor 1C Cyclin-dependent kinase inhibitor p57 p57Kip2 CDKN1C KIP2 protein protein human Homo sapiens Human MSDASLRSTSTMERLVARGTFPVLVRTSACRSLFGPVDHEELSRELQARLAELNAEDQNRWDYDFQQDMPLRGPGRLQWTEVDSDSVPAFYRETVQVGRCRLLLAPRPVAVAVAVSPPLEPAAESLDGLEEAPEQLPSVPVPAPASTPPPVPVLAPAPAPAPAPVAAPVAAPVAVAVLAPAPAPAPAPAPAPAPVAAPAPAPAPAPAPAPAPAPAPDAAPQESAEQGANQGQRGQEPLADQLHSGISGRPAAGTAAASANGAAIKKLSGPLISDFFAKRKRSAPEKSSGDVPAPCPSPSAAPGVGSVEQTPRKRLR A94CCBF09F8CB3E4 ccne1_human G1/S-specific cyclin-E1 CCNE1 CCNE protein protein human Homo sapiens Human MPRERRERDAKERDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPWDNNAVCADPCSLIPTPDKEDDDRVYPNSTCKPRIIAPSRGSPLPVLSWANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA DIP protein P24864 original sequence version: 114 DIP protein P24864 original sequence version: 150 424DF0B253B7047E mpip2_human M-phase inducer phosphatase 2 Dual specificity phosphatase Cdc25B CDC25B CDC25HU2 protein protein human Homo sapiens Human MEVPQPEPAPGSALSPAGVCGGAQRPGHLPGLLLGSHGLLGSPVRAAASSPVTTLTQTMHDLAGLGSETPKSQVGTLLFRSRSRLTHLSLSRRASESSLSSESSESSDAGLCMDSPSPMDPHMAEQTFEQAIQAASRIIRNEQFAIRRFQSMPVRLLGHSPVLRNITNSQAPDGRRKSEAGSGAASSSGEDKENDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKTLEKEEEKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKRRRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVDKFVIVDCRYPYEYEGGHIKTAVNLPLERDAESFLLKSPIAPCSLDKRVILIFHCEFSSERGPRMCRFIRERDRAVNDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFKDELKTFRLKTRSWAGERSRRELCSRLQDQ EDE24B0E84AC1BE3 cdn1a_human Cyclin-dependent kinase inhibitor 1 CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1 p21 CDK-interacting protein 1 Melanoma differentiation-associated protein 6 protein protein human Homo sapiens Human MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP DIP protein P38936 original sequence version: 120 DIP protein P38936 original sequence version: 165 DIP protein P38936 original sequence version: 160 98D1E7C519ADFCA9 cdk3_human Cyclin-dependent kinase 3 CDK3 Cell division protein kinase 3 CDKN3 protein protein human Homo sapiens Human MDMFQKVEKIGEGTYGVVYKAKNRETGQLVALKKIRLDLEMEGVPSTAIREISLLKELKHPNIVRLLDVVHNERKLYLVFEFLSQDLKKYMDSTPGSELPLHLIKSYLFQLLQGVSFCHSHRVIHRDLKPQNLLINELGAIKLADFGLARAFGVPLRTYTHEVVTLWYRAPEILLGSKFYTTAVDIWSIGCIFAEMVTRKALFPGDSEIDQLFRIFRMLGTPSEDTWPGVTQLPDYKGSFPKWTRKGLEEIVPNLEPEGRDLLMQLLQYDPSQRITAKTALAHPYFSSPEPSPAARQYVLQRFRH DIP protein Q00526 original sequence version: 154 1128BE0096EA262A ccnd2_human G1/S-specific cyclin-D2 CCND2 protein protein human Homo sapiens Human MELLCHEVDPVRRAVRDRNLLRDDRVLQNLLTIEERYLPQCSYFKCVQKDIQPYMRRMVATWMLEVCEEQKCEEEVFPLAMNYLDRFLAGVPTPKSHLQLLGAVCMFLASKLKETSPLTAEKLCIYTDNSIKPQELLEWELVVLGKLKWNLAAVTPHDFIEHILRKLPQQREKLSLIRKHAQTFIALCATDFKFAMYPPSMIATGSVGAAICGLQQDEEVSSLTCDALTELLAKITNTDVDCLKACQEQIEAVLLNSLQQYRQDQRDGSKSEDELDQASTPTDVRDIDL E4E5FEF476D76D90 cdk2-ccne2-1 2 10 unspecified role unspecified role bait bait P24941:p.Lys33Met mutation mutation certain certain sequence position certain certain certain sequence position certain K M Wed May 02 17:27:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys33met' Tue Jun 30 16:41:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys33Met' 7 unspecified role unspecified role prey prey physical association physical association CDK2 is catalytically inactive. We used a catalytically inactive mutant of CDK2, CDK2K33M, to search for additional CDK2-interacting protein(s) by the yeast two-hybrid assay. cdk2-cks1-1 2 10 unspecified role unspecified role bait bait P24941:p.Lys33Met mutation mutation certain certain sequence position certain certain certain sequence position certain K M Wed May 02 16:35:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys33met' Tue Jun 30 17:35:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys33Met' 6 unspecified role unspecified role prey prey physical association physical association CDK2 is catalytically inactive. We used a catalytically inactive mutant of CDK2, CDK2K33M, to search for additional CDK2-interacting protein(s) by the yeast two-hybrid assay. cdk2-cdkn3-2 2 10 unspecified role unspecified role bait bait P24941:p.Lys33Met mutation mutation certain certain sequence position certain certain certain sequence position certain K M Wed May 02 15:04:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys33met' Tue Jun 30 18:09:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys33Met' 12 unspecified role unspecified role prey prey physical association physical association CDK2 is catalytically inactive. We used a catalytically inactive mutant of CDK2, CDK2K33M, to search for additional CDK2-interacting protein(s) by the yeast two-hybrid assay. cdk2-ccnd1-1 2 10 unspecified role unspecified role bait bait P24941:p.Lys33Met mutation mutation certain certain sequence position certain certain certain sequence position certain K M Wed May 02 16:58:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys33met' Tue Jun 30 16:58:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys33Met' 11 unspecified role unspecified role prey prey physical association physical association CDK2 is catalytically inactive. We used a catalytically inactive mutant of CDK2, CDK2K33M, to search for additional CDK2-interacting protein(s) by the yeast two-hybrid assay. cdk2-ccnd2-1 2 10 unspecified role unspecified role bait bait P24941:p.Lys33Met mutation mutation certain certain sequence position certain certain certain sequence position certain K M Wed May 02 17:16:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys33met' Tue Jun 30 19:36:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys33Met' 18 unspecified role unspecified role prey prey physical association physical association CDK2 is catalytically inactive. We used a catalytically inactive mutant of CDK2, CDK2K33M, to search for additional CDK2-interacting protein(s) by the yeast two-hybrid assay. cdk2-ccnh-1 2 9 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait P24941:p.Lys33Met mutation mutation certain certain sequence position certain certain certain sequence position certain K M Wed May 02 15:28:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys33met' Tue Jun 30 17:53:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys33Met' physical association physical association CDK2 is catalytically inactive. We used a catalytically inactive mutant of CDK2, CDK2K33M, to search for additional CDK2-interacting protein(s) by the yeast two-hybrid assay. cdk2-cdkn1a-1 2 10 unspecified role unspecified role bait bait P24941:p.Lys33Met mutation mutation certain certain sequence position certain certain certain sequence position certain K M Wed May 02 18:04:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys33met' Tue Jun 30 18:12:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys33Met' 16 unspecified role unspecified role prey prey physical association physical association CDK2 is catalytically inactive. We used a catalytically inactive mutant of CDK2, CDK2K33M, to search for additional CDK2-interacting protein(s) by the yeast two-hybrid assay. cdk2-cdc25b 2 10 unspecified role unspecified role bait bait P24941:p.Lys33Met mutation mutation certain certain sequence position certain certain certain sequence position certain K M Wed May 02 15:28:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys33met' Tue Jun 30 17:24:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys33Met' 15 unspecified role unspecified role prey prey physical association physical association CDK2 is catalytically inactive. We used a catalytically inactive mutant of CDK2, CDK2K33M, to search for additional CDK2-interacting protein(s) by the yeast two-hybrid assay. cdk2-ccne2-2 5 7 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait P24941:p.Lys33Met mutation mutation certain certain sequence position certain certain certain sequence position certain K M Wed May 02 14:30:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys33met' Tue Jun 30 19:53:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys33Met' physical association physical association CDK2 is catalytically inactive. In addition, a novel protein, encoded by clone HT376, was identifed and confrmed to strongly interact with CDK2K33M after re-transformation of the rescued plasmid into yeast cells with CDK2K33M as bait (Figure 1a). 1a cdk3-ccne2-1 5 17 unspecified role unspecified role bait bait Q00526:p.Lys33Met mutation mutation certain certain sequence position certain certain certain sequence position certain K M Wed May 02 18:50:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys33met' Tue Jun 30 18:34:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys33Met' 7 unspecified role unspecified role prey prey physical association physical association CDK2 is catalytically inactive. When tested in yeast cells, both the catalytically inactive CDK3K33M mutant and wild type CDK3 interact with cyclin E2 (Figure 1a), suggesting that cyclin E2 could potentially function as an activating cyclin subunit of CDK3 and that CDK3-cyclin E2 is functionally distinct from the CDK2-cyclin E2 complex. 1a cdk2-ccne2-3 4 10 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Total cell lysates were prepared from CT10 and HSF43 and sequentially immunoprecipitated with the cyclin E2 antibody and immunoblotted (IP-Western) with either anti-cyclin E2 or anti-CDK2 antibody (Figure 2b). 2b A competing cyclin E2 antigen peptide cdkn1a-ccne2-1 5 7 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association All three CIP/KIP inhibitors interact with cyclin E2 as determined by the two-hybrid assay in yeast cells, 3A cdkn1b-ccne2-1 5 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait cyclin bind region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association All three CIP/KIP inhibitors interact with cyclin E2 as determined by the two-hybrid assay in yeast cells, 3A cdkn1c-ccne2-1 5 13 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association All three CIP/KIP inhibitors interact with cyclin E2 as determined by the two-hybrid assay in yeast cells, 3A cdkn1b-ccne2-2 3 8 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Cyclin E2 was readily detected in p27 immunocomplex (Figure 3b), demon- strating an in vivo association between cyclin E2 and CDK inhibitor. 3b cdk2-ccne2-4 1 10 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association The level of cyclin E2 protein as well as cyclin E2-CDK2 complexes in these cells were determined by IP-Western analysis (Figure 7a). 7a A competing cyclin E2 antigen peptide The half-life of the short lived Cyclin E2 protein is increased by expression of the viral E6 and E7 oncoproteins. cdk2-ccne1-4 1 14 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association 7b A competing cyclin E2 antigen peptide The half-life of the short lived Cyclin E1 protein is increased by expression of the viral E7 oncoproteins.