IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A retinoblastoma-binding protein that affects cell-cycle control and confers transforming ability. A retinoblastoma-binding protein that affects cell-cycle control and confers transforming ability. Nat. Genet. (1061-4036) 1998 imex curation Woitach JT., Zhang M., Niu CH., Thorgeirsson SS. Cancer - Interactions investigated in the context of cancer The species of the pRb construct used was ascertained as the plasmid pASRb2 encodes human pRb (Huntley et al., 1998 Plant Mol Biol 37, 155-169). snorri_thorgeirsson@nih.gov 10-APR-2007: Contacted by JYOTI. orchard Only protein-protein interactions rat-b5t Rattus norvegicus RLE (rat liver epithelial) (not sensitive to TGF-beta) B5T cell line b5t RLE (rat epithelial liver) B5T (not sensitive to TGF-beta) cell line These cells have a higher level of BOG than the RLE phi13 cell line. B5T cells have higher levels of BOG than RLE phi13 cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Woitach JT., Zhang M., Niu CH., Thorgeirsson SS. Cancer - Interactions investigated in the context of cancer The species of the pRb construct used was ascertained as the plasmid pASRb2 encodes human pRb (Huntley et al., 1998 Plant Mol Biol 37, 155-169). snorri_thorgeirsson@nih.gov Accepted 2007-APR-19 by ORCHARD. 10-APR-2007: Contacted by JYOTI. Nat. Genet. (1061-4036) 1998 orchard Only protein-protein interactions imex curation A retinoblastoma-binding protein that affects cell-cycle control and confers transforming ability. A retinoblastoma-binding protein that affects cell-cycle control and confers transforming ability. Nat. Genet. (1061-4036) 1998 imex curation Woitach JT., Zhang M., Niu CH., Thorgeirsson SS. Cancer - Interactions investigated in the context of cancer The species of the pRb construct used was ascertained as the plasmid pASRb2 encodes human pRb (Huntley et al., 1998 Plant Mol Biol 37, 155-169). snorri_thorgeirsson@nih.gov 10-APR-2007: Contacted by JYOTI. orchard Only protein-protein interactions rat-rle_phi13 Rattus norvegicus RLE (rat liver epithelial) sensitive to TGF-beta phi13 cell line rle_phi13 RLE (rat epithelial liver) phi 13 sensitive to TGF-beta non-transformed cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Woitach JT., Zhang M., Niu CH., Thorgeirsson SS. Cancer - Interactions investigated in the context of cancer There is not UniProtKb entry for rat p107. Thus this protein was created with the mouse protein as a cross-reference. Revisit as and when there is a UniProtKb entry for this protein. The species of the pRb construct used was ascertained as the plasmid pASRb2 encodes human pRb (Huntley et al., 1998 Plant Mol Biol 37, 155-169). snorri_thorgeirsson@nih.gov Accepted 2007-APR-19 by ORCHARD. 10-APR-2007: Contacted by JYOTI. Nat. Genet. (1061-4036) 1998 orchard Only protein-protein interactions imex curation A retinoblastoma-binding protein that affects cell-cycle control and confers transforming ability. A retinoblastoma-binding protein that affects cell-cycle control and confers transforming ability. Nat. Genet. (1061-4036) 1998 imex curation Woitach JT., Zhang M., Niu CH., Thorgeirsson SS. Cancer - Interactions investigated in the context of cancer The species of the pRb construct used was ascertained as the plasmid pASRb2 encodes human pRb (Huntley et al., 1998 Plant Mol Biol 37, 155-169). snorri_thorgeirsson@nih.gov 10-APR-2007: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Woitach JT., Zhang M., Niu CH., Thorgeirsson SS. Cancer - Interactions investigated in the context of cancer The species of the pRb construct used was ascertained as the plasmid pASRb2 encodes human pRb (Huntley et al., 1998 Plant Mol Biol 37, 155-169). snorri_thorgeirsson@nih.gov Accepted 2007-APR-19 by ORCHARD. 10-APR-2007: Contacted by JYOTI. Nat. Genet. (1061-4036) 1998 orchard Only protein-protein interactions imex curation rbbp9_rat Serine hydrolase RBBP9 Rbbp9 Bog B5T-overexpressed gene protein Retinoblastoma-binding protein 9 protein protein rat Rattus norvegicus (Rat) Rat MASPTKAVIVPGNGGGDVATHGWYGWVRKGLEQIPGFQCLAKNMPDPITARESIWLPFMETELHCDEKTIIIGHSSGAIAAMRYAETHQVYALILVSAYTSDLGDENERASGYFSRPWQWEKIKANCPHIIQFGSTDDPFLPWKEQQEVADRLDAKLYKFTDRGHFQNTEFHELIRVVKSMLTPAL 41EC9779708E5E19 e2f1_rat Transcription factor E2F1 E2f1 protein protein rat Rattus norvegicus (Rat) Rat MAVAPAGGQHAPALEALLGAGALRLLDSSQIVIISTAPDVGAPQVPTGPAAPPAGPRDPDVLLFATPQAPRPAPSAPRPALGRPPVKRRLDLETDHQYLAGSSGPFRGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSHSADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIAKKSKNHIQWLGSRTMVGIGQRLEGLTQDLQQLQESEQQLDHLMHICTTQLQLLSEDSDIQRLAYVTCQDLRSIADPAEQMVIVIKAPPETQLQAVDSAETFQISLKSKQGPIDVFLCPEESAEGISPGRTSYQETSGEDRNADSGTAGPPPSPPSTSPTLDPSQSLLGLEQEAVLPRIGNLRAPMEEDRLSPLVAADSLLEHVKEDFSGLLPGEFISLSPPHEAVDYHFGLEEGEGIRDLFDCDFGDLTPLDF 4E401ADF6E20C13C rb_rat Retinoblastoma-associated protein pRb pp105 Rb1 Rb-1 protein protein rat Rattus norvegicus (Rat) Rat MPPKAPRRTAAAEPPPPPPPPPEDDPAQDSDPEELPLIRLEFEKIEEPEFIALCQKLKVPDHVRERAWLTWEKVSSVDGILEGYIQKKKELWGICIFIAAVDLDEMPFTFTELQKSIETSVYKFFDLLKEIDTSTKVDNAVSRLLKKYNVLCALYSKLERTCGLIYLTQPSSGLSTEINSMLVLKVSWITFLLAKGEVVQMEDDLVISFQLMLCVLDYFIKLSPPALLREPYKTAATPINGSPRTPRRGQNRSARIAKQLESDTRTIEVLCKEHECNVDEVKNVYFKNFIPFISSLGIVSSNGLPELESLSKRYEEVYLKSKDLDARLFLDHDKTLQTDTIDSFETERTPRKSNPDEEANMVTPHTPVRTVMNTIQQLMVILNSASDQPSENLISYFSNCTVNPKENILKRVKDVGHIFKEKFASAVGQGCIDIGAQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSMLQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKVEANLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDGEGPDHLESACSLSLPLQSNHTAADMYLSPIRSPKKRTSTTRVNSAANTETQAASAFHTQKPLKSTSLSLFYKKVYRLAYLRLNTLCARLLSDHPELEHIIWTLFQHTLENEYELMKDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAAQETFKRVLIREEEFDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFSSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGGNPPKPLKKLRFDIEGSDEADGSKHLPAESKFQQKLAEMTSTRTRMQKQKLNDSMEISNKEEK 3BA735EA59927301 rbl2_rat Retinoblastoma-like protein 2 Rbl2 Retinoblastoma-related protein 2 130 kDa retinoblastoma-associated protein pRb2 PPAR-alpha-interacting complex protein 128 protein protein rat Rattus norvegicus (Rat) Rat MASGGNQSSPPPPAAAASSEEEEEDGDTADRAQPAGSPSHQIQQRFEELCSRLNMDEAARAEAWSSYRSMSESYTLEGNDLHWLACALYVACRKSVPTVSKGTAEGNYVSLTRILRCSEQSLIEFFNKMKKWEDMANLPPHFRERTERLERNFTVSAVIFKKYEPIFQDIFKYPQEEQPRQQRGRKQRRQPCTTSEIFHFCWVLFIYAKGNFPMISDDLVNSYHLLLCALDLVYGNALQCSNRKELVNPNFKGVSEDGHPRDSHPSSDPPCVIEKLCSLHDGLVLEAKGIKQHFWKPYIRKLFEKKLLRGKEENLTGFLEPGNFAESFKAVNKAYEEYVLATGSLDERIFLGEDAEEEVGTFSRCVSAASGTESAERTQMRDILQQHLDKSKTLRVCNPLTGVRYVQENSPCVTPVSTATHSLNRLHTMLAGLRNAPSEKLEQILRSCSRDPTRAIADRLREMYEIYSQHFQPDENVSNCAKEMANKHFRFAEMLYYKVLESVIEQEQKRLGDMDLSGVLEQDAFHKSLLACCLEVVAFSYKPPGNFPFIAEIFDVPHYHFYKVIEVFIRAEDGLCREVVKHLNQIEEQILDHLAWKTKSPLWDRIRDNENRVPTCEEVTPPHNLERTDEIYIAGSPLTPRRVGEVRTDAGGLGRSVTSPTTLYDRYSSPTVSTTRRRLFESDSPSEGSTAGRIPPQPLVNAVPVQNVSGETVSVTPVPGQTLVTMATATVTANNGQTVTIPVQGIANENGGITFFPVQVNVGGQAQAVTGSIQPLSAQALAGSLSSQQVTGTTLQVPGPVAIQQISPGGQQQNQGQPLTSSSIRPRKTSSLSLFFRKVYYLAGVRLRDLCTKLDISDELRKKIWTCFEFSIVQCPELMMDRHLDQLLMCAIYVMAKVTKEDKSFQNIMRCYRTQPQARSQVYRSVLIKGKRRNSGSCENRSHQNSPTELNTDRASRDSSPVMRSNSTLPVPQPSSAPPTPTRLTGANSDIEEEERGDLIQFYNNIYRKQIQTFAMKYSQANSQMDTPPLSPYPFVRTGSPRRVQLSQSHPIYISPHKNEAMLSPREKIFYYFSNSPSKRLREINSMIRTGETPTKKRGILLDDGSESPAKRICPENHSALLRRLQDVANDRGSH 6C42A87E163B4298 rb_human Retinoblastoma-associated protein pRb pp110 p105-Rb RB1 p110-RB1 protein protein human Homo sapiens Human MPPKTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPLVRLEFEETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGICIFIAAVDLDEMSFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVLFALFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQLMLCVLDYFIKLSPPMLLKEPYKTAVIPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDARLFLDHDKTLQTDSIDSFETQRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSASDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNSTANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCERLLSEHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFKRVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFPSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKHLPGESKFQQKLAEMTSTRTRMQKQKMNDSMDTSNKEEK DIP protein P06400 original sequence version: 102 DIP protein P06400 original sequence version: 195 DIP protein P06400 original sequence version: 204 DIP protein P06400 original sequence version: 126 DIP protein P06400 original sequence version: 151 DIP protein P06400 original sequence version: 153 DIP protein P06400 original sequence version: 181 DIP protein P06400 original sequence version: 184 DIP protein P06400 original sequence version: 213 DIP protein P06400 original sequence version: 208 DIP protein P06400 original sequence version: 174 DIP protein P06400 original sequence version: 212 DIP protein P06400 original sequence version: 179 DIP protein P06400 original sequence version: 146 C8E746111E19CC32 d3zs62_rat Rbl1 protein protein rat Rattus norvegicus (Rat) Rat MFEDEPHAEGAAAVAAAREALQALCQELNLDEGSAAEALDDFTAIRGNYSLEGEVIHWLACSLYVACRKSIIPTVGKGVMEGNCVSLTRILRSAKLSLIQFFSKMKKWMDMSNLPQEFRERIERLERNFEVSTVIFKKFEPIFLDIFQNPYEELPKLPRSRKQRRIPCSVKDLFNFCWTLFVYTKGNFRMIGDDLVNSYHLLLCCLDLIFANAIMCPNRRDLLNPSFKGLPSDFHAVNFKAAEEPPCIIAVLCDLHDGLLVEAKGIKEHYFKPYISKLFDKKILKGECLLDLSSFTDNSKAVNKEYEEYVLTVGDFDERIFLGADAEEEIGTPRKFAADTQFGKLTSQASVDCNLQQHFEKKRSFAPSTPLTGRRYLQEKEAVTTPVASATQSVSRLQSIVAGLKSAPSEQLLTIFESCMRNPMGNIVKIVKGIGETFCQHYTQSTDKQPGSHIDFAVNRLKLAEILYYKILETIMVQETRRLHGMDMSVLLEQDIFHRSLLACCLEIVLFAYSSPRTFPWIIDVLGLQPFYFYKVIEVVIRSEEGLSRDMVKHLNSIEEQILESLAWTNNSALWEALRASANKVPSCEEVIFPNNFEIGNGGSVQGHLPMMPMSPIIHPRVKEVRTDSGSLRKDMQPLSPISVHERYSSPAAGSAKRRLFGDDPPKETLMEKIMAEGTQLKIAPSSVTAESLSISPGQALLTMATTTVTGTTGRKVTVPLHGIANDAGEITLVPISMNTTQDSTAESLVSLTAQSLIGASPKQTHLTKTQDAPLTGISKPKRTGSLALFYRKVYHLASVRLRDLCLKLDVSNELRRKIWTCFEFTLVHCPDLMKDRHLDQLLLCAFYIMAKVTKEERTFQEIMKSYRNQPQANSHVYRSVLLKSIPGEVVAYNGDYEMTDGDIEDATKTPNCSSEPVKEERGDLIKFYNAIYVGRVKSFALKYDLSNQDHIMDAPPLSPFPHIKQQPGSPRRISQQHSIYVSPHKNASGLTPRSALLYKFNGSPSKSRKVLRNMARQGSRKAKKRVIAISGDAESPAKRLCQENDDVLLKRLQDVVSERANH The sequence has been withdrawn from uniprot. E98265ACDFB07467 lt_sv40 Large T antigen protein protein sv40 sv40 SV40 MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDKGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLFCSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLICKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETKCDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQAVDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLPKMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLNFELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKRTQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALLLMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDDDEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFTCFKKPPTPPPEPET 3E37D4BAFC5D59BD rb1-rbbp9 3 8 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association 2 A To test for a possible interaction between Bog and Rb, we used the yeast two-hybrid system (Fig. 2a). The intensity of X-gal staining for Rb and Bog as compared with Rb and SV40 large T suggested a similar degree of interaction. rb1-tala 3 10 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 2 A To test for a possible interaction between Bog and Rb, we used the yeast two-hybrid system (Fig. 2a). The intensity of X-gal staining for Rb and Bog as compared with Rb and SV40 large T suggested a similar degree of interaction. rb1-rbbp9-1 2 4 unspecified role unspecified role prey prey region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O88350:p.Leu63Glu mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L E Wed May 02 16:39:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu63glu' Tue Jun 30 16:07:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu63Glu' 6 unspecified role unspecified role bait bait physical association physical association 2 C Altering a crucial leucine in the Rb binding site abolishes the ability of Bog to interact with Rb The disruption of the interaction by the mutation leu63glu in Bog indicates that Bog interacts with Rb through the LXCXE sequence. rbbp9-rb1 2 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 2 B, D Rb could be precipitated with both anti-Bog and anti-Rb rb1-e2f1-1 2 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 2 B, C Rb could be precipitated with both anti-Bog and anti-Rb, but E2F-1 was detected only in samples precipitated with anti-Rb. The authors state that in the B5T cells the amount of E2F1 in complex with pRb is dramatically reduced as compared to phi13 cells due to higher levels of BOG, which displaces E2F1 from complex with pRb. Incubation with Bog-MBP disrupts this interaction, but if Bog is mutated in the Rb-binding site it does not. rb1-rbbp9-2 1 4 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 2 B rbbp9-rb1-1 1 4 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 2 B Rb could be precipitated with both anti-Bog and anti-Rb, but E2F-1 was detected only in samples precipitated with anti-Rb. e2f1-rb1 2 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 2 B, C The authors state that in the B5T cells the amount of E2F1 in complex with pRb is dramatically reduced as compared to phi13 cells due to higher levels of BOG, which displaces E2F1 from complex with pRb. Incubation with Bog-MBP disrupts this interaction, but if Bog is mutated in the Rb-binding site it does not. The Rb/E2F-1 complexes were immunoprecipitated with either anti-Rb or anti-E2F-1 antibodies, and incubated with the fusion protein MBP-Bog or the mutated MBP-BogDeltaQ. rbbp9-rbl2 2 7 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 2 D Bog is present in complexes with all the Rb family members rbl1_rat_prot-rbbp9 2 9 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 2 D Bog is present in complexes with all the Rb family members