IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176. NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1998 imex curation Ling L., Cao Z., Goeddel DV. In reference [16] which is stated to describe the IkappaB-alpha expression vector it is stated that the expression vectors for IkappaB-alpha was provided by Dr. Takeshi Kurama. A paper by Dr Kurama describes this expression vector as of human origin (Huang t et al J Biol Chem, Vol. 275, Issue 13, 9501-9509, March 31, 2000 PMID 107340980). goeddel@tularik.com 22-Jan-2008: Contacted by JYOTI. davet Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Ling L., Cao Z., Goeddel DV. In reference [16] which is stated to describe the IkappaB-alpha expression vector it is stated that the expression vectors for IkappaB-alpha was provided by Dr. Takeshi Kurama. A paper by Dr Kurama describes this expression vector as of human origin (Huang t et al J Biol Chem, Vol. 275, Issue 13, 9501-9509, March 31, 2000 PMID 107340980). goeddel@tularik.com Accepted 2007-DEC-21 by DAVET. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1998 davet Only protein-protein interactions imex curation m3k14_human Mitogen-activated protein kinase kinase kinase 14 MAP3K14 NIK Serine/threonine-protein kinase NIK NF-kappa-beta-inducing kinase protein protein human Homo sapiens Human MAVMEMACPGAPGSAVGQQKELPKAKEKTPPLGKKQSSVYKLEAVEKSPVFCGKWEILNDVITKGTAKEGSEAGPAAISIIAQAECENSQEFSPTFSERIFIAGSKQYSQSESLDQIPNNVAHATEGKMARVCWKGKRRSKARKKRKKKSSKSLAHAGVALAKPLPRTPEQESCTIPVQEDESPLGAPYVRNTPQFTKPLKEPGLGQLCFKQLGEGLRPALPRSELHKLISPLQCLNHVWKLHHPQDGGPLPLPTHPFPYSRLPHPFPFHPLQPWKPHPLESFLGKLACVDSQKPLPDPHLSKLACVDSPKPLPGPHLEPSCLSRGAHEKFSVEEYLVHALQGSVSSGQAHSLTSLAKTWAARGSRSREPSPKTEDNEGVLLTEKLKPVDYEYREEVHWATHQLRLGRGSFGEVHRMEDKQTGFQCAVKKVRLEVFRAEELMACAGLTSPRIVPLYGAVREGPWVNIFMELLEGGSLGQLVKEQGCLPEDRALYYLGQALEGLEYLHSRRILHGDVKADNVLLSSDGSHAALCDFGHAVCLQPDGLGKSLLTGDYIPGTETHMAPEVVLGRSCDAKVDVWSSCCMMLHMLNGCHPWTQFFRGPLCLKIASEPPPVREIPPSCAPLTAQAIQEGLRKEPIHRVSAAELGGKVNRALQQVGGLKSPWRGEYKEPRHPPPNQANYHQTLHAQPRELSPRAPGPRPAEETTGRAPKLQPPLPPEPPEPNKSPPLTLSKEESGMWEPLPLSSLEPAPARNPSSPERKATVPEQELQQLEIELFLNSLSQPFSLEEQEQILSCLSIDSLSLSDDSEKNPSKASQSSRDTLSSGVHSWSSQAEARSSSWNMVLARGRPTDTPSYFNGVKVQIQSLNGEHLHIREFHRVKVGDIATGISSQIPAAAFSLVTKDGQPVRYDMEVPDSGIDLQCTLAPDGSFAWSWRVKHGQLENRP DIP protein Q99558 original sequence version: 159 C9D10F67FF7F48AC ikka_human Inhibitor of nuclear factor kappa-B kinase subunit alpha CHUK IKKA TCF16 I-kappa-B kinase 1 Conserved helix-loop-helix ubiquitous kinase Nuclear factor NF-kappa-B inhibitor kinase alpha Transcription factor 16 protein protein human Homo sapiens Human MERPPGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIKSCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPEELNILIHDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKIIHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMSGEVRFSSHLPQPNSLCSLVVEPMENWLQLMLNWDPQQRGGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSAKIISFLLPPDESLHSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLISASQQLKAKLEFFHKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYAEVGVIGYLEDQIMSLHAEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPSDHSYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGAVTPQTSAWLPPTSAEHDHSLSCVVTPQDGETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDWSWLTE DIP protein O15111 original sequence version: 91 DIP protein O15111 original sequence version: 123 DIP protein O15111 original sequence version: 133 DIP protein O15111 original sequence version: 118 DIP protein O15111 original sequence version: 125 DIP protein O15111 original sequence version: 96 4EA55C6FFC66FA16 ikkb_human Inhibitor of nuclear factor kappa-B kinase subunit beta IKBKB IKKB I-kappa-B kinase 2 Nuclear factor NF-kappa-B inhibitor kinase beta Serine/threonine protein kinase IKBKB protein protein human Homo sapiens Human MSWSPSLTTQTCGAWEMKERLGTGGFGNVIRWHNQETGEQIAIKQCRQELSPRNRERWCLEIQIMRRLTHPNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRKYLNQFENCCGLREGAILTLLSDIASALRYLHENRIIHRDLKPENIVLQQGEQRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWHSKVRQKSEVDIVVSEDLNGTVKFSSSLPYPNNLNSVLAERLEKWLQLMLMWHPRQRGTDPTYGPNGCFKALDDILNLKLVHILNMVTGTIHTYPVTEDESLQSLKARIQQDTGIPEEDQELLQEAGLALIPDKPATQCISDGKLNEGHTLDMDLVFLFDNSKITYETQISPRPQPESVSCILQEPKRNLAFFQLRKVWGQVWHSIQTLKEDCNRLQQGQRAAMMNLLRNNSCLSKMKNSMASMSQQLKAKLDFFKTSIQIDLEKYSEQTEFGITSDKLLLAWREMEQAVELCGRENEVKLLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKPRDQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVEEVVSLMNEDEKTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNASRLSQPGQLMSQPSTASNSLPEPAKKSEELVAEAHNLCTLLENAIQDTVREQDQSFTALDWSWLQTEEEEHSCLEQAS DIP protein O14920 original sequence version: 89 DIP protein O14920 original sequence version: 100 DIP protein O14920 original sequence version: 121 DIP protein O14920 original sequence version: 126 DIP protein O14920 original sequence version: 136 DIP protein O14920 original sequence version: 128 DIP protein O14920-1 original sequence version: 151 DIP protein O14920 original sequence version: 142 DIP protein O14920 original sequence version: 159 DIP protein O14920 original sequence version: 93 DIP protein O14920 original sequence version: 124 DIP protein O14920-1 original sequence version: 178 DIP protein O14920 original sequence version: 85 F9CADF671AE9E14E ikba_human NF-kappa-B inhibitor alpha NFKBIA IKBA MAD3 NFKBI I-kappa-B-alpha Major histocompatibility complex enhancer-binding protein MAD3 protein protein human Homo sapiens Human MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL DIP protein P25963 original sequence version: 160 DIP protein P25963 original sequence version: 157 DIP protein P25963 original sequence version: 145 DIP protein P25963 original sequence version: 170 DIP protein P25963 original sequence version: 122 DIP protein P25963 original sequence version: 128 DIP protein P25963 original sequence version: 78 DIP protein P25963 original sequence version: 121 DIP protein P25963 original sequence version: 124 088B313226786395 chuk-chuk-1 1 3 putative self putative self putative self putative self region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O15111:p.Lys44Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A Wed May 02 19:03:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys44ala' Tue Jun 30 18:54:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys44Ala' O15111:p.Ser176Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:03:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser176ala' Tue Jun 30 19:33:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser176Ala' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. phosphorylation phosphorylation reaction phosphorylation true 1 A, 4 A The epitope-tagged proteins were immunoprecipitated with an anti-FLAG antibody, and incubated with [g-32P]ATP. In these assays, wild-type IKK-a, IKK-b, and NIK become autophosphorylated when expressed individually, while mutants of all three kinases containing lysine-to-alanine (KA) substitutions in their ATP-binding sites were not autophosphorylated IKK-alpha underwent autophosphorylation. chuk-nfkbia 1 5 enzyme target enzyme target substrate substrate unspecified role unspecified role ecoli Escherichia coli (strain K12) 3 enzyme enzyme unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O15111:p.Lys44Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:42:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys44ala' Tue Jun 30 16:18:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys44Ala' O15111:p.Ser176Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 14:35:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser176ala' Tue Jun 30 17:10:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser176Ala' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. phosphorylation phosphorylation reaction phosphorylation 1 A IkappaB-alpha was phosphorylated. NIK - Coexpression of NIK with IKK-alpha stimulated IKK-alpha phosphorylation of IkappaB-alpha but did not stimulate IKK-alpha(S176A) to the same extent. ikbkb-nfkbia 1 4 enzyme enzyme unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O14920:p.Lys44Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A Wed May 02 15:44:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys44ala' Tue Jun 30 19:33:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys44Ala' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 5 enzyme target enzyme target substrate substrate unspecified role unspecified role ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 1 A, 4 B IkappaB-alpha was phosphorylated. The ser177ala mutation in IKK-beta (equivalent to ser176ala in IKK-alpha) did not reduce autophosphorylation or phosphorylation of IkappaB-alpha. mutation of the equivalent serine in IKK-b results in a kinase, IKK-b(S177A), that is fully active in autophosphorylation and in phosphorylation of IkB-a ikbkb-ikbkb-1 1 4 putative self putative self putative self putative self region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O14920:p.Lys44Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A Wed May 02 19:27:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys44ala' Tue Jun 30 18:08:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys44Ala' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. phosphorylation phosphorylation reaction phosphorylation true 1 A, 4 B IKK-beta was auphosphorylated. The epitope-tagged proteins were immunoprecipitated with an anti-FLAG antibody, and incubated with [g-32P]ATP. In these assays, wild-type IKK-a, IKK-b, and NIK become autophosphorylated when expressed individually, while mutants of all three kinases containing lysine-to-alanine (KA) substitutions in their ATP-binding sites were not autophosphorylated The ser177ala mutation in IKK-beta (equivalent to ser176ala in IKK-alpha) did not reduce autophosphorylation or phosphorylation of IkappaB-alpha. mutation of the equivalent serine in IKK-b results in a kinase, IKK-b(S177A), that is fully active in autophosphorylation and in phosphorylation of IkB-a map3k14-map3k14 1 2 putative self putative self putative self putative self region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q99558:p.[Lys429Ala;Lys430Ala] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain K A Wed May 02 17:56:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys429ala,lys430ala' Tue Jun 30 16:45:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys429Ala;Lys430Ala]' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. phosphorylation phosphorylation reaction phosphorylation true 1 A The epitope-tagged proteins were immunoprecipitated with an anti-FLAG antibody, and incubated with [g-32P]ATP. In these assays, wild-type IKK-a, IKK-b, and NIK become autophosphorylated when expressed individually, while mutants of all three kinases containing lysine-to-alanine (KA) substitutions in their ATP-binding sites were not autophosphorylated NIK is autophosphorylated. map3k14-chuk-1 1 3 enzyme target enzyme target substrate substrate unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined lys44ala biological feature biological feature certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O15111:p.Ser176Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 14:58:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser176ala' Tue Jun 30 18:06:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser176Ala' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 2 enzyme enzyme unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q99558:p.[Lys429Ala;Lys430Ala] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain K A Wed May 02 19:48:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys429ala,lys430ala' Tue Jun 30 19:22:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys429Ala;Lys430Ala]' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. phosphorylation phosphorylation reaction phosphorylation 1 B, 3 To examine its ability to phosphorylate IKK-a and IKK-b, we coexpressed NIK with the catalytically inactive IKK mutants. We found that NIK can phosphorylate IKK-a(KA), but only weakly phosphorylates IKK-b(KA) IKK-alpha (lys44ala) is catalytically inactive. IKK-alpha is phosphorylated. Ser-176 represents the major site of IKK-alpha phosphorylation by NIK. map3k14-ikbkb-1 1 4 enzyme target enzyme target substrate substrate unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined lys44ala biological feature biological feature certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 2 enzyme enzyme unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q99558:p.[Lys429Ala;Lys430Ala] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain K A Wed May 02 20:08:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys429ala,lys430ala' Tue Jun 30 16:33:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys429Ala;Lys430Ala]' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. phosphorylation phosphorylation reaction phosphorylation 1 B To examine its ability to phosphorylate IKK-a and IKK-b, we coexpressed NIK with the catalytically inactive IKK mutants. We found that NIK can phosphorylate IKK-a(KA), but only weakly phosphorylates IKK-b(KA) IKK-beta (lys44ala) is only weakly phosphorylated. IKK-beta (lys44ala) is phosphorylated. IKK-beta(KA) (lys44ala) is catalytically inactive. chuk-nfkbia-2 1 5 enzyme target enzyme target substrate substrate unspecified role unspecified role ecoli Escherichia coli (strain K12) 3 enzyme enzyme unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O15111:p.Ser176Glu mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S E Wed May 02 19:46:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser176glu' Tue Jun 30 16:19:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser176Glu' human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 phosphorylation phosphorylation reaction phosphorylation 7 A IkappaB-alpha was phosphorylated. At equivalent levels of expression, IKK-a(S176E) had significantly greater kinase activity than IKK-a, as measured by either autophosphorylation or phosphorylation of IkB-a chuk-chuk-2 1 3 enzyme enzyme unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O15111:p.Ser176Glu mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S E Wed May 02 15:40:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser176glu' Tue Jun 30 16:39:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser176Glu' human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 3 enzyme target enzyme target substrate substrate unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O15111:p.Ser176Glu mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S E Wed May 02 17:33:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser176glu' Tue Jun 30 16:18:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser176Glu' human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 phosphorylation phosphorylation reaction phosphorylation 7 A IkappaB-alpha was phosphorylated. At equivalent levels of expression, IKK-a(S176E) had significantly greater kinase activity than IKK-a, as measured by either autophosphorylation or phosphorylation of IkB-a NIK - The activity of wild-type IKK-alpha was strongly enhanced by coexpression with NIK. IKK-alpha(S176E) was similarly active when expressed either with or without NIK, suggesting that IKK-alpha(S176E) activity is independent of upstream activation.