IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ An activity-dependent network of interactions links the Rel protein Dorsal with its cytoplasmic regulators. An activity-dependent network of interactions links the Rel protein Dorsal with its cytoplasmic regulators. Development (0950-1991) 1997 Edwards DN., Towb P., Wasserman SA. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Edwards DN., Towb P., Wasserman SA. Accepted 2007-OCT-21 by ORCHARD. Development (0950-1991) 1997 orchard An activity-dependent network of interactions links the Rel protein Dorsal with its cytoplasmic regulators. An activity-dependent network of interactions links the Rel protein Dorsal with its cytoplasmic regulators. Development (0950-1991) 1997 Edwards DN., Towb P., Wasserman SA. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Edwards DN., Towb P., Wasserman SA. Accepted 2007-OCT-21 by ORCHARD. ovarian and embryonic cDNA libraries Development (0950-1991) 1997 orchard An activity-dependent network of interactions links the Rel protein Dorsal with its cytoplasmic regulators. An activity-dependent network of interactions links the Rel protein Dorsal with its cytoplasmic regulators. Development (0950-1991) 1997 Edwards DN., Towb P., Wasserman SA. orchard drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Edwards DN., Towb P., Wasserman SA. Accepted 2007-OCT-21 by ORCHARD. Development (0950-1991) 1997 orchard kpel_drome Serine/threonine-protein kinase pelle CG5974 pll protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSGVQTAEAEAQAQNQANGNRTRSRSHLDNTMAIRLLPLPVRAQLCAHLDALDVWQQLATAVKLYPDQVEQISSQKQRGRSASNEFLNIWGGQYNHTVQTLFALFKKLKLHNAMRLIKDYVSEDLHKYIPRSVPTISELRAAPDSSAKVNNGPPFPSSSGVSNSNNNRTSTTATEEIPSLESLGNIHISTVQRAAESLLEIDYAELENATDGWSPDNRLGQGGFGDVYRGKWKQLDVAIKVMNYRSPNIDQKMVELQQSYNELKYLNSIRHDNILALYGYSIKGGKPCLVYQLMKGGSLEARLRAHKAQNPLPALTWQQRFSISLGTARGIYFLHTARGTPLIHGDIKPANILLDQCLQPKIGDFGLVREGPKSLDAVVEVNKVFGTKIYLPPEFRNFRQLSTGVDVYSFGIVLLEVFTGRQVTDRVPENETKKNLLDYVKQQWRQNRMELLEKHLAAPMGKELDMCMCAIEAGLHCTALDPQDRPSMNAVLKRFEPFVTD 4B29E2B40ACB81A8 cact_drome NF-kappa-B inhibitor cactus CG5848 cact protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MPSPTKAAEAATKATATSDCSCSAASVEQRAPSNAANPSSSLATSGKIGGKTQDQTAAINKQKEFAVPNETSDSGFISGPQSSQIFSEEIVPDSEEQDKDQQESAPQKEQPVVLDSGIIDEEEDQEEQEKEEEHQDTTTATADSMRLKHSADTGIPQWTVESHLVSRGEQLNNLGQSSSTQITGRSKVQSSTASTANANPSGSGATSSAPPSSINIMNAWEQFYQQNDDGDTPLHLACISGSVDVVAALIRMAPHPCLLNIQNDVAQTPLHLAALTAQPNIMRILLLAGAEPTVRDRHGNTALHLSCIAGEKQCVRALTEKFGATEIHEAHRQYGHRSNDKAVSSLSYACLPADLEIRNYDGERCVHLAAEAGHIDILRILVSHGADINAREGKSGRTPLHIAIEGCNEDLANFLLDECEKLNLETATYAGLTAYQFACIMNKSRMQNILEKRGAETVTPPDSDYDSSDIEDLDDTKMYDRFGDPRYFVSYNGGNPMTVA 9E9711B97CDB499F dors_drome Embryonic polarity protein dorsal dl CG6667 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MFPNQNNGAAPGQGPAVDGQQSLNYNGLPAQQQQQLAQSTKNVRKKPYVKITEQPAGKALRFRYECEGRSAGSIPGVNSTPENKTYPTIEIVGYKGRAVVVVSCVTKDTPYRPHPHNLVGKEGCKKGVCTLEINSETMRAVFSNLGIQCVKKKDIEAALKAREEIRVDPFKTGFSHRFQPSSIDLNSVRLCFQVFMESEQKGRFTSPLPPVVSEPIFDKKAMSDLVICRLCSCSATVFGNTQIILLCEKVAKEDISVRFFEEKNGQSVWEAFGDFQHTDVHKQTAITFKTPRYHTLDITEPAKVFIQLRRPSDGVTSEALPFEYVPMDSGKHTFWNLHRHLKRKPDEDLFQQILRLDAKREVQPPTIEVIDLDTPKIDVQREIPSEMEFNHEESQQSEPALEQEQSVQQEQYTQEQSLQQEQYTQEQSLQQEQYLQQLEQQQSFQLEEPMQQDQELPAQQSFDQAIDHLPDHTSDHIPEDMEAADAHAEAEAHRLRSEQEKEIDTIIDEKVRELEQLDLGQQLEPRPLTANDKITEWMKSSEIEQQVHEPSPTAEADVLDSALEISKADKTLDELLETVAELDEIYTDFKVQRDTYKNTIQNELAGLQGRAPLQVEDSFDDAATYTSLQIAFKNPVLIPMDDIMPPTPPMSQCAPEDAHQHYDPVEVNSQARKPETPMRPVPPVPPAILTIQYPPEEDKLPPLPPKRIRKQDSNAENRSIEANTVQTKPSTGESPLNKRLPPAPKNPNFNTLPRQKKPGFFSKLFSRRKSKPDLAQGQENSSILDSKANSREPSIGHFNMQDPMRASLRSSKSAAPFISNPAPAKSSPVKAKKPGSKLTKPVGRSVSSVSGKRPAYLNADVVHIPLKGDSVNSLPQQQRTEGYSQSSTISVGAGLDRRTASALQLADIPISEGGMELVAIADRQSLHNLVSSIEGHFNVQLDPNLDLTEAEHFALYTSIPPLAAASEFDETSAYYAPVDAGEILTPDEVAKRLAAANGI DIP protein P15330 original sequence version: 136 E29C6594AC07D662 flna_drome Filamin-A cher Actin-binding protein 280 Filamin-1 Filamin1 cheerio sko CG3937 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MEAERDLAEDAQWKKIQQNTFTRWANEHLKTIDRSINNLETDLSDGLRLIALIEVLSQKRMPKYNKRPTFRSQKLENVSVALKFLQDEGIKIVNIDSSDIVDCKLKLILGLIWTLILHYSISMPMWDGEDDKQLNGSGHTPKQRLLNWIHAKIPDLPINNFTNDWTTGKAVGALVDACAPGLCPDWELWDPKDAVQNASEAMGLADDWLNVRQLIKPEELVNPNVDEQSMMTYLSQYPNSKLKTGAPLRPKTNPNRVRAYGPGIEPIGPVVGAPANFTVETFSAGKGSVDVDIQGPNGEIEKADVRFNNDKNLTYTVSYIPKSEGSHKVAVKFSGRDIPKSPFPVKVEGHAGDASKVKVTGPGIQPNGVTIKKPTFFDILAKDAGRGVPEVIIIDPANHKTSVAAKVRQLENDTWRCEYVTALQGLHSVNVFYAGTPIPNSPFPVKVAPLSDARKVRASGRGLQATGVRVGDDADFKIYTEGAGEGEPEVRVIGPGGMNQNVMQSKVDGNTYECHYYPTKEGRYVIMVTFAGQEVAKSPFEVKVGPKKESSIVAYGPGLSSGVIGYPAAFVVETNGETGALGFTVAGPSQAEIECHDNGDGSALVKYHPTAVGEYAVHILCDNEDIPKSPFIAQILPRTDFHPELVKASGPGLEKNGVTINQPTSFTVDPSKAGNAPLDVVVQDVFGTKLPVELKNNPDGTKKVTYTPTSGVPHTVEVNYGGVSTPNSPHRVYVGVPVDAAKVQAFGPWLQPGVRPNAATHFNVDAREAGDAELKVKIIHEETKIEVPCRIIDNEDNTYSVEVIPPSKGAYTTTMTYGGQRVPLGEKVVVEQTVDVSKIKVDGLEPSVIMNAATDFMVDMSKVGSNIDSGKLSCAIFDPMGHVLPSKIVQGPTDDIFRIMYTPFEAGRHTIELMYDNIPVPGSPFVVNVKSGCDPARCKAYGPGLEKGLTNQKNKFTVETKGAGNGGLSLAIEGPSEAKMTCTDNRDGSCDVDYLATDPGEYDITIRFADKHIPGSPFRVLVEETVDPSKVKVYGPGIEHGQVRESVPTFFNVDVGEAGPGRIAVKLTNSEGIPVDNLRVEDKGNCIYAVHYVPPKAGSVLTCQVKFSEVEVPCSPFVMTVFPKSEPTKVKVKGVNEKKKTPASLPAEFEIDTKQAGQADINVAIKNPKGKAMQPRLEEVSTGTYVVSFVPDECGTYQCSIKYGDKEIEGSPFKLEAFPTGEAKKCKLVEQAPKIQTSGSQSHLKVDAREAGDGAVTCKITNKAGSEIVDIDVIEKDGFFDILYALNDPGDYDINVKFGGKDIPNGSFSIKAVESIEQYSHSEYIEEHTTKVVQQTTQSELVNGKSEITYRSVAFEKLPLPTTGGNVTAEVRMPSGKVDKPVIQDNRDGTVSVKYDPREEGSHELVVKYNGEPVQGSPFKFHVDSITSGYVTAYGPGLTHGVTGEPANFTISTKGASAGGLTMAVEGPSKADINYHDNKDGTVSVQYLPTAPGEYQVSVRFGDKHIKGSPYFAKITGEGRKRNQISVGSCSEVTMPGDITDDDLRALNASIQAPSGLEEPCFLKRMPTGNIGISFTPREIGEHLVSVKRLGKHINNSPFKVTVCEREVGDAKKVKVSGTGLKEGQTHADNIFSVDTRNAGFGGLSVSIEGPSKAEIQCTDKDDGTLNISYKPTEPGYYIVNLKFADHHVEGSPFTVKVAGEGSNRKREKIQRERDAVPITEIGSQCKLTFKMPGITSFDLAACVTSPSNVTEDAEIQEVEDGLYAVHFVPKELGVHTVSVRYSEMHIPGSPFQFTVGPLRDSGSHLVKAGGSGLERGVVGEAAEFNVWTREAGGGSLAISVEGPSKADIEFKDRKDGSCDVSYKVTEPGEYRVGLKFNDRHIPDSPFKVYVSPDAGDAHKLEVQQFPQGNIQADAPYQFMVRKNGAKGELDAKIVAPSGTDDDCFIQVIDGEMYSVRFYPRENGIHAIHVKFNGVHIPDSPFRIKVGKDVADPAAVHASGNGLDEVKTGHKADFIINTCNAGVGTLAVSIDGPSKVAMDCTEVEEGYKVRYTPLLPGEHYITVKYNNMHIVGSPFKVNATGDKLADEGAQETSTVIVETVQKVAKGGKNTGVHLPTFKSDASKVVSKGMGLKKAYIGKQNQFSISATDAGNNILYVGMYGPKGPCEEFHVKHAGHNNYNVQYLVRDRGQYVLLIKWGEEHIPGSPFQIDV A600974971A3FD84 tube_drome Protein Tube CG10520 tub tube protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAYGWNGCGMGVQVNGSNGAIGLSSKYSRNTELRRVEDNDIYRLAKILDENSCWRKLMSIIPKGMDVQACSGAGCLNFPAEIKKGFKYTAQDVFQIDEAANRLPPDQSKSQMMIDEWKTSGKLNERPTVGVLLQLLVQAELFSAADFVALDFLNESTPARPVDGPGALISLELLEEEMEVDNEGLSLKYQSSTATLGADAQGSVGLNLDNFEKDIVRRDKSVPQPSGNTPPIAPPRRQQRSTTNSNFATLTGTGTTSTTIPNVPNLTILNPSEQIQEPVLQPRPMNIPDLSILISNSGDLRATVSDNPSNRTSSTDPPNIPRITLLIDNSGDVNSRPNHAPAKASTATTSTASSNNLPMISALNISKGSRETLRPESRSSSSSLSKDDDDDNDGEEDGEEEYPDAFLPNLSNSEQQSSNNDSSLTTVTGTSGDNSFELTNDSSSTSNDDYACNIPDLSELQQ DDFBB9D72B8036FC toll_drome Protein toll Tl CG5490 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSRLKAASELALLVIILQLLQWPGSEASFGRDACSEMSIDGLCQCAPIMSEYEIICPANAENPTFRLTIQPKDYVQIMCNLTDTTDYQQLPKKLRIGEVDRVQMRRCMLPGHTPIASILDYLGIVSPTTLIFESDNLGMNITRQHLDRLHGLKRFRFTTRRLTHIPANLLTDMRNLSHLELRANIEEMPSHLFDDLENLESIEFGSNKLRQMPRGIFGKMPKLKQLNLWSNQLHNLTKHDFEGATSVLGIDIHDNGIEQLPHDVFAHLTNVTDINLSANLFRSLPQGLFDHNKHLNEVRLMNNRVPLATLPSRLFANQPELQILRLRAELQSLPGDLFEHSTQITNISLGDNLLKTLPATLLEHQVNLLSLDLSNNRLTHLPDSLFAHTTNLTDLRLEDNLLTGISGDIFSNLGNLVTLVMSRNRLRTIDSRAFVSTNGLRHLHLDHNDIDLQQPLLDIMLQTQINSPFGYMHGLLTLNLRNNSIIFVYNDWKNTMLQLRELDLSYNNISSLGYEDLAFLSQNRLHVNMTHNKIRRIALPEDVHLGEGYNNNLVHVDLNDNPLVCDCTILWFIQLVRGVHKPQYSRQFKLRTDRLVCSQPNVLEGTPVRQIEPQTLICPLDFSDDPRERKCPRGCNCHVRTYDKALVINCHSGNLTHVPRLPNLHKNMQLMELHLENNTLLRLPSANTPGYESVTSLHLAGNNLTSIDVDQLPTNLTHLDISWNHLQMLNATVLGFLNRTMKWRSVKLSGNPWMCDCTAKPLLLFTQDNFERIGDRNEMMCVNAEMPTRMVELSTNDICPAEKGVFIALAVVIALTGLLAGFTAALYYKFQTEIKIWLYAHNLLLWFVTEEDLDKDKKFDAFISYSHKDQSFIEDYLVPQLEHGPQKFQLCVHERDWLVGGHIPENIMRSVADSRRTIIVLSQNFIKSEWARLEFRAAHRSALNEGRSRIIVIIYSDIGDVEKLDEELKAYLKMNTYLKWGDPWFWDKLRFALPHRRPVGNIGNGALIKTALKGSTDDKLELIKPSPVTPPLTTPPAEATKNPLVAQLNGVTPHQAIMIANGKNGLTNLYTPNGKSHGNGHINGAFIINTNAKQSDV DIP protein P08953 original sequence version: 128 D1BFC42245E3EABE dl-tub-1 1 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey physical association physical association 2, Table 1 we confirmed the interactions between Tube and Pelle and between Dorsal and Cactus dl-dl-1 1 6 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2, Table 1 we confirmed the interactions between Tube and Pelle and between Dorsal and Cactus dl-cact 1 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2, Table 1 we confirmed the interactions between Tube and Pelle and between Dorsal and Cactus dl-pll-1 1 4 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2, Table 1 we confirmed the interactions between Tube and Pelle and between Dorsal and Cactus dl-cact-2 1 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey physical association physical association 2, Table 1 we confirmed the interactions between Tube and Pelle and between Dorsal and Cactus cact-cact 1 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey physical association physical association 2, Table 1 we confirmed the interactions between Tube and Pelle and between Dorsal and Cactus pll-tub-2 1 8 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2, Table 1 we confirmed the interactions between Tube and Pelle and between Dorsal and Cactus pll-dl 1 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 2, Table 1 we confirmed the interactions between Tube and Pelle and between Dorsal and Cactus dl-tub-2 1 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2, Table 1 we confirmed the interactions between Tube and Pelle and between Dorsal and Cactus pll-tub-3 1 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-1393804]The shifted ranges (25-256) couldn't be applied as the new full feature sequence is different from the previous one. [EBI-1393804]25-257 [EBI-1393804]P22812,3 4 unspecified role unspecified role prey prey physical association physical association 2, Table 1 we confirmed the interactions between Tube and Pelle and between Dorsal and Cactus dl-cact-3 3 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 3 We observed coimmunoprecipitation of Dorsal with both anti-Cactus and anti-Tube sera dl-tub-3 3 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 3 We observed coimmunoprecipitation of Dorsal with both anti-Cactus and anti-Tube sera dl-cact-4 3 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association 3 We observed coimmunoprecipitation of Dorsal with both anti-Cactus and anti-Tube sera tub-cact 3 5 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 3 We observed coimmunoprecipitation of Dorsal with both anti-Cactus and anti-Tube sera tub-cher 1 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 5 The Filamin DNA-binding domain fusion failed to interact with activation domain fusions for Pelle, Dorsal and Cactus, but had a readily detectable interaction (five-fold above background) with a Tube activation domain fusion tl-cher 1 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 5 The Toll/IL-1 receptor homology domain appears to be both necessary and sufficient for the interaction of Toll with Filamin tl-cher-1 2 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Toll829-1036 interacting clones encode portions of a Drosophila homolog of ABP280 or Filamin, an actin-binding protein that localizes to the inner surface of the cell membrane