IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches. The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches. Curr. Biol. (0960-9822) 1997 imex curation Winter D., Podtelejnikov AV., Mann M., Li R. Extract was first fractionated by two ion-exchange columns, followed by fractionation through a nickel column which binds to the histidine tag, and then further purification by gel filtration. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast chromatography chromatography technology column chromatography chromatography fingerprinting peptide massfingerprinting fingerprinting Winter D., Podtelejnikov AV., Mann M., Li R. Extract was first fractionated by two ion-exchange columns, followed by fractionation through a nickel column which binds to the histidine tag, and then further purification by gel filtration. Accepted 2007-JUN-8 by DAVET. Curr. Biol. (0960-9822) 1997 Only protein-protein interactions imex curation arp3_yeast Actin-related protein 3 ARP3 ACT4 YJR065C J1760 Actin-like protein ARP3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSYLNNPAVVMDNGTGLTKLGFAGNDSPSWVFPTAIATAAPSNTKKSSGVGAPSAVSNEASYFGNSTSATNFNGATGGLLSNNLSGKRGTEDLDFYIGNEALVASQGPSYSLSYPIRHGQVENWDHMERFWENSIFKYLRTEPEDHFFLLTEPPLNPPENREQVAEIFFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVIDSGDGVTHVIPVAEGYVIGSAIKNIPIAGRDITLFIQSLLRERGEADTSLRTAEKIKQEYCYVCPDIVKEFNKFDKDPSKFAQFVVENQEKTRRKVVDIGYERFLAPEIFFNPEIASSDFLTPLPTVVDQTIQACPIDVRKGLYNNIVLSGGSTMFKDFGRRLQRDLKSIVNNRIAQSELLSGTKSTGVDVSVISHRKQRNAVWFGGSLLAQTAEFKGYCHTKKDYEEYGPEIVRNFSLFNMV 5BBD87E83F476E9F 5BBD87E83F476E9F arpc3_yeast Actin-related protein 2/3 complex subunit 3 ARC18 YLR370C L8039.15 Arp2/3 complex 21 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MPAYHSTFPVDPNTDRMVGNFALLPLNTKFRGPAYPSNSDYDIIDECLDLFRANSFFKNFEIKSPADRVLIYGILFINDCLAHLKITTSFNEAVKVLTNVALDNFTLPGTPGFPLNNVYQVPVQDHNSMDLLKTYIQQFRQELAMRLLERVYSSTDSKEYPSKFWLAFTRRRFMNKSL 456EABC2B9CC4860 456EABC2B9CC4860 arpc4_yeast Actin-related protein 2/3 complex subunit 4 ARC19 YKL013C YKL166 Arp2/3 complex 20 kDa protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQSLRPYLTAVRYSLEAALTLSNFSSQEVERHNRPEVEVPNTSAELLLQPMHISRNENEQVLIEPSVNSVRMSLMVKQADEIEQILVHKFTRFLEQRAEAFYILRRVPIPGYSISFLITNKHTESMKTGKLVDFIIEFMEDVDKEISEIKLFLNARARFVAEAYLDEFVY B80CBD6642E39C1C B80CBD6642E39C1C arp2_yeast Actin-related protein 2 ACT2 YDL029W D2778 ARP2 Actin-like protein ARP2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDPHNPIVLDQGTGFVKIGRAGENFPDYTFPSIVGRPILRAEERASVATPLKDIMIGDEASEVRSYLQISYPMENGIIKNWTDMELLWDYAFFEQMKLPSTSNGKILLTEPPMNPLKNREKMCEVMFEKYDFGGVYVAIQAVLALYAQGLSSGVVVDSGDGVTHIVPVYESVVLSHLTRRLDVAGRDVTRHLIDLLSRRGYAFNRTADFETVRQIKEKLCYVSYDLDLDTKLARETTALVESYELPDGRTIKVGQERFEAPECLFQPGLVDVEQPGVGELLFNTVQSADVDIRSSLYKAIVLSGGSSMYPGLPSRLEKELKQLWFSRVLHNDPSRLDKFKVRIEDPPRRKHMVFIGGAVLASIMADKDHMWLSKQEWQESGPSAMTKFGPR 4C9E8952B477A1B9 4C9E8952B477A1B9 arpc5_yeast Actin-related protein 2/3 complex subunit 5 ARC15 Arp2/3 complex 16 kDa subunit YIL062C protein protein yeast Saccharomyces cerevisiae Baker's yeast MEADWRRIDIDAFDPESGRLTAADLVPPYETTVTLQELQPRMNQLRSLATSGDSLGAVQLLTTDPPYSADAPTKEQYFKSVLEALTQVRQADIGNVIKNLSDSQRDVLVKYLYKGMSVPQGQKQGGVLLAWLERITQVSGVTPIVHYISDRRTV 10E4DAF0F0801B02 10E4DAF0F0801B02 arpc2_yeast Actin-related protein 2/3 complex subunit 2 ARC35 YNR035C N3296 Arp2/3 complex 34 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MLHLQPQNLLIQKTLNEAIEALRKGSPLTMDRIVSDFDYTTYHISNTAEDKSILLLSVKTKAWVSVSECQLDGSLTLLKFLADHYSSLGGVTIPSEVEPGYDYTLQITLAELVQESILQLSVLKTIILSFPFELAISKFIELSQQQPAPVEAEITGGEVAANGDNTLFTIKYRDEENIFIKPSNDRVTIIFETIFQDETDKIFGKVFLQEFVDARKRNRQIQSAPQVLYSHEPPLELKRLYQPPKVAEQSRRFITFVLFPRHFQTKELQFHSICQLTLFRNYFHYHIKCSKAYMHSRMRFRVDSFIKVLNRAKVDEDDENDELSAEGRQQARRTFTGRKIVY 9AF71A93688729F6 9AF71A93688729F6 arp2-arp3-1 1 4 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 2 unspecified role unspecified role bait bait region His_Myc tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Identification of the subunits of the Arp2/3 complex by mass spectrometry 5,6,7