IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A role for the transcription factors Mbp1 and Swi4 in progression from G1 to S phase. A role for the transcription factors Mbp1 and Swi4 in progression from G1 to S phase. Science (0036-8075) 1993 Koch C., Moll T., Neuberg M., Ahorn H., Nasmyth K. Column purified on a heparin agarose column and eluted with an ammonium sulfate gradient. Fractions containing the peak of MBF were further purified by DNA affinity chromatography with MCB elements from the TMPl promoter. Swi6 identified by comigration on a gel with a previously purified protein sample, Mbp1 was identified by peptide mapping. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom partial id prot seq partial identification of protein sequence partial id prot seq Koch C., Moll T., Neuberg M., Ahorn H., Nasmyth K. Column purified on a heparin agarose column and eluted with an ammonium sulfate gradient. Fractions containing the peak of MBF were further purified by DNA affinity chromatography with MCB elements from the TMPl promoter. Swi6 identified by comigration on a gel with a previously purified protein sample, Mbp1 was identified by peptide mapping. Accepted 2007-AUG-5 by JYOTI. Science (0036-8075) 1993 A role for the transcription factors Mbp1 and Swi4 in progression from G1 to S phase. A role for the transcription factors Mbp1 and Swi4 in progression from G1 to S phase. Science (0036-8075) 1993 Koch C., Moll T., Neuberg M., Ahorn H., Nasmyth K. Column purified on a heparin agarose column and eluted with an ammonium sulfate gradient. Fractions containing the peak of MBF were further purified by DNA affinity chromatography with MCB elements from the TMPl promoter. Swi6 identified by comigration on a gel with a previously purified protein sample, Mbp1 was identified by peptide mapping. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa partial id prot seq partial identification of protein sequence partial id prot seq Koch C., Moll T., Neuberg M., Ahorn H., Nasmyth K. Column purified on a heparin agarose column and eluted with an ammonium sulfate gradient. Fractions containing the peak of MBF were further purified by DNA affinity chromatography with MCB elements from the TMPl promoter. Swi6 identified by comigration on a gel with a previously purified protein sample, Mbp1 was identified by peptide mapping. Accepted 2007-AUG-5 by JYOTI. Science (0036-8075) 1993 swi6_yeast Regulatory protein SWI6 SWI6 YLR182W L9470.8 Cell-cycle box factor subunit SWI6 Trans-acting activator of HO endonuclease gene MBF subunit P90 protein protein yeast Saccharomyces cerevisiae Baker's yeast MALEEVVRYLGPHNEIPLTLTRDSETGHFLLKHFLPILQQYHDTGNINETNPDSFPTDEERNKLLAHYGIAVNTDDRGELWIELEKCLQLLNMLNLFGLFQDAFEFEEPETDQDEEDPSHSKLPENKTKSENSKDNISSKRINNLQDMSLDSDAHRELGSPLKKLKIDTSVIDAESDSTPNTARGKPNDDINKGPSGDNENNGTDDNDRTAGPIITFTHDLTSDFLSSPLKIMKALPSPVVNDNEQKMKLEAFLQRLLFPEIQEMPTSLNNDSSNRNSEGGSSNQQQQHVSFDSLLQEVNDAFPNTQLNLNIPVDEHGNTPLHWLTSIANLELVKHLVKHGSNRLYGDNMGESCLVKAVKSVNNYDSGTFEALLDYLYPCLILEDSMNRTILHHIIITSGMTGCSAAAKYYLDILMGWIVKKQNRPIQSGTNEKESKPNDKNGERKDSILENLDLKWIIANMLNAQDSNGDTCLNIAARLGNISIVDALLDYGADPFIANKSGLRPVDFGAGTSKLQNTNGGDENSKMVSKGDYDGQKNGKAKKIRSQLLKNPPETTSLINDVQNLLNSISKDYENETVQYNEKLEKLHKELNEQREELANSREQLANVKQLKDEYSLMQEQLTNLKAGIEEEEESFREESKKLGIIADESSGIDWDSSEYDADEPFKVEFLSDFLEDKLQKNYEGDISKLLEAESKEQIMEQIRNQLPAEKIQSMLPPTVLLKARINAYKRNDKHLTNVLDTISTKQSELENKFRRVLSLCLKIDENKVDNMLDGLLQAISSEDPQDIDTDEMQDFLKKHAS 9B317FCACEEC493C 9B317FCACEEC493C mbp1_yeast Transcription factor MBP1 YDL056W MBP1 MBF subunit p120 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNQIYSARYSGVDVYEFIHSTGSIMKRKKDDWVNATHILKAANFAKAKRTRILEKEVLKETHEKVQGGFGKYQGTWVPLNIAKQLAEKFSVYDQLKPLFDFTQTDGSASPPPAPKHHHASKVDRKKAIRSASTSAIMETKRNNKKAEENQFQSSKILGNPTAAPRKRGRPVGSTRGSRRKLGVNLQRSQSDMGFPRPAIPNSSISTTQLPSIRSTMGPQSPTLGILEEERHDSRQQQPQQNNSAQFKEIDLEDGLSSDVEPSQQLQQVFNQNTGFVPQQQSSLIQTQQTESMATSVSSSPSLPTSPGDFADSNPFEERFPGGGTSPIISMIPRYPVTSRPQTSDINDKVNKYLSKLVDYFISNEMKSNKSLPQVLLHPPPHSAPYIDAPIDPELHTAFHWACSMGNLPIAEALYEAGTSIRSTNSQGQTPLMRSSLFHNSYTRRTFPRIFQLLHETVFDIDSQSQTVIHHIVKRKSTTPSAVYYLDVVLSKIKDFSPQYRIELLLNTQDKNGDTALHIASKNGDVVFFNTLVKMGALTTISNKEGLTANEIMNQQYEQMMIQNGTNQHVNSSNTDLNIHVNTNNIETKNDVNSMVIMSPVSPSDYITYPSQIATNISRNIPNVVNSMKQMASIYNDLHEQHDNEIKSLQKTLKSISKTKIQVSLKTLEVLKESSKDENGEAQTNDDFEILSRLQEQNTKKLRKRLIRYKRLIKQKLEYRQTVLLNKLIEDETQATTNNTVEKDNNTLERLELAQELTMLQLQRKNKLSSLVKKFEDNAKIHKYRRIIREGTEMNIEEVDSSLDVILQTLIANNNKNKGAEQIITISNANSHA BB7C35E29802BBD5 BB7C35E29802BBD5 mbp-probe MBP containing probe - TMP1 gene dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid chemical synthesis Chemical synthesis (Chemical synthesis) MBP probe consisting of the TMPl promoter carrying two MCB elements swi6-mbp1-1 1 4 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component physical association physical association MBF contains Swi6 and a 120-kilodalton protein (p120). MBF was purified and the gene encoding p120 (termed MBPI) was cloned. Figure 1 swi6-mbp1-2 2 4 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component association association Cotranslation of Mbpl and Swi6 not only decreased the amount of binary Mbp1-DNA complexes but also generated new protein-DNA complexes which comigrate with MBF from yeast (Fig. 4B, lane 6). Figure 4 mbp1-mbp-dna-1 2 5 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association The NH2-terminal 124 residues of MBP1 were translated in vitro and tested for binding to the TIMP1 promoter. Figure 4B