IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Isolation and characterization of the Saccharomyces cerevisiae SDH4 gene encoding a membrane anchor subunit of succinate dehydrogenase. Isolation and characterization of the Saccharomyces cerevisiae SDH4 gene encoding a membrane anchor subunit of succinate dehydrogenase. J. Biol. Chem. (0021-9258) 1994 imex curation Bullis BL., Lemire BD. Mitochondria isolated then protein complexes separated by blue native gel electrophoresis. Proteins then separated in the second dimension by SDS-PAGE. SDH1 and SDH2 identified by western blot, SDH1, SDH3 and SDH4 identifed by Edman sequencing. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast bn-page blue native page bn-page western blot western blot Immuno blot Immuno blot Bullis BL., Lemire BD. Mitochondria isolated then protein complexes separated by blue native gel electrophoresis. Proteins then separated in the second dimension by SDS-PAGE. SDH1 and SDH2 identified by western blot, SDH1, SDH3 and SDH4 identifed by Edman sequencing. Accepted 2007-MAY-10 by JYOTI. J. Biol. Chem. (0021-9258) 1994 Only protein-protein interactions imex curation sdh3_yeast Succinate dehydrogenase [ubiquinone] cytochrome b subunit, mitochondrial SDH3 CYB3 YKL141W YKL4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAMMVKLGLNKSALLLKPSAFSRAAALSSSRRLLFNTARTNFLSTSPLKNVASEMNTKAAIAEEQILNKQRAKRPISPHLTIYQPQLTWYLSSLHRISLVLMGLGFYLFTILFGVSGLLGLGLTTEKVSNWYHQKFSKITEWSIKGSFAYLFAIHYGGAIRHLIWDTAKELTLKGVYRTGYALIGFTAVLGTYLLTL F210FE99AE97607A sdha_yeast Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial SDH1 SDHA YKL148C YKL602 Flavoprotein subunit of complex II protein protein yeast Saccharomyces cerevisiae Baker's yeast MLSLKKSALSKLTLLRNTRTFTSSALVRQTQGSVNGSASRSADGKYHIIDHEYDCVVIGAGGAGLRAAFGLAEAGYKTACISKLFPTRSHTVAAQGGINAALGNMHKDNWKWHMYDTVKGSDWLGDQDSIHYMTREAPKSIIELEHYGVPFSRTENGKIYQRAFGGQTKEYGKGAQAYRTCAVADRTGHALLHTLYGQALRHDTHFFIEYFALDLLTHNGEVVGVIAYNQEDGTIHRFRAHKTIIATGGYGRAYFSCTSAHTCTGDGNAMVSRAGFPLQDLEFVQFHPSGIYGSGCLITEGARGEGGFLVNSEGERFMERYAPTAKDLACRDVVSRAITMEIREGRGVGKKKDHMYLQLSHLPPEVLKERLPGISETAAIFAGVDVTKEPIPIIPTVHYNMGGIPTKWNGEALTIDEETGEDKVIPGLMACGEAACVSVHGANRLGANSLLDLVVFGRAVAHTVADTLQPGLPHKPLPSDLGKESIANLDKLRNANGSRSTAEIRMNMKQTMQKDVSVFRTQSSLDEGVRNITAVEKTFDDVKTTDRSMIWNSDLVETLELQNLLTCASQTAVSAANRKESRGAHAREDYPNRDDEHWMKHTLSWQKDVAAPVTLKYRRVIDHTLDEKECPSVPPTVRAY A657E440B8ED58E2 A657E440B8ED58E2 sdhb_yeast Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial YLL041C SDH2 SDH SDHB Iron-sulfur subunit of complex II protein protein yeast Saccharomyces cerevisiae Baker's yeast MLNVLLRRKAFCLVTKKGMATATTAAATHTPRLKTFKVYRWNPDEPSAKPHLQSYQVDLNDCGPMVLDALLKIKDEQDSTLTFRRSCREGICGSCAMNIGGRNTLACICKIDQNESKQLKIYPLPHMFIVKDLVPDLTNFYQQYKSIQPYLQRSSFPKDGTEVLQSIEDRKKLDGLYECILCACCSTSCPSYWWNQEQYLGPAVLMQAYRWLIDSRDQATKTRKAMLNNSMSLYRCHTIMNCTRTCPKGLNPGLAIAEIKKSLAFA C14170F3670F6930 C14170F3670F6930 dhsd_yeast Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial SDH4 ACN18 YDR178W YD9395.11 Succinate-ubiquinone reductase membrane anchor subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MMLPRSMKFMTGRRIFHTATVRAFQSTAKKSLTIPFLPVLPQKPGGVRGTPNDAYVPPPENKLEGSYHWYMEKIFALSVVPLATTAMLTTGPLSTAADSFFSVMLLGYCYMEFNSCITDYISERVYGVWHKYAMYMLGLGSAVSLFGIYKLETENDGVVGLVKSLWDSSEKDNSQKIEAKK 80D76A32E7E6DA70 sdh1-sdh2-1 1 3 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component 2 unspecified role unspecified role neutral component neutral component physical association physical association subunits. We purified succinate dehydrogenase by blue native gel electrophoresis, determined the amino-terminal sequence of the Sdh4p subunit and used this information to clone the SDH4 gene. 1