IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Several endoplasmic reticulum stress proteins, including ERp72, interact with thyroglobulin during its maturation. Several endoplasmic reticulum stress proteins, including ERp72, interact with thyroglobulin during its maturation. J. Biol. Chem. (0021-9258) 1994 Kuznetsov G., Chen LB., Nigam SK. No contact email address given. DAVET rat-frtl_5 Rattus norvegicus thyroid cell line FRTL-5 frtl_5 Rat thyroid cell line FRTL-5 This thyroid cell line has been established from normal thyroid glands from 5 to 6-week-old Fischer rats. FRTL-5 is a clonal line of the continuously cultured FRTL cells derived by successive clonal isolation. The cells are characterised by maintaining highly differentiated thyroid features such as secretion of thyroglobulin and concentration of iodide. They display a sensitivity to thyroid-stimulating hormone (TSH)-induced increase in cAMP levels detecting TSH as absolute growth requirement. density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Kuznetsov G., Chen LB., Nigam SK. No contact email address given. Accepted 2007-Not a month!-12 by DAVET. J. Biol. Chem. (0021-9258) 1994 DAVET Several endoplasmic reticulum stress proteins, including ERp72, interact with thyroglobulin during its maturation. Several endoplasmic reticulum stress proteins, including ERp72, interact with thyroglobulin during its maturation. J. Biol. Chem. (0021-9258) 1994 Kuznetsov G., Chen LB., Nigam SK. No contact email address given. DAVET rat-frtl_5 Rattus norvegicus thyroid cell line FRTL-5 frtl_5 Rat thyroid cell line FRTL-5 This thyroid cell line has been established from normal thyroid glands from 5 to 6-week-old Fischer rats. FRTL-5 is a clonal line of the continuously cultured FRTL cells derived by successive clonal isolation. The cells are characterised by maintaining highly differentiated thyroid features such as secretion of thyroglobulin and concentration of iodide. They display a sensitivity to thyroid-stimulating hormone (TSH)-induced increase in cAMP levels detecting TSH as absolute growth requirement. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kuznetsov G., Chen LB., Nigam SK. No contact email address given. Immunoprecipitation was performed both on lysates from uncross-linked and cross-linked cells. A unique UniProtKB entry could not be associated with PDI since the exact gene product of the interactor cannot be ascertained. Accepted 2007-Not a month!-12 by DAVET. J. Biol. Chem. (0021-9258) 1994 DAVET Several endoplasmic reticulum stress proteins, including ERp72, interact with thyroglobulin during its maturation. Several endoplasmic reticulum stress proteins, including ERp72, interact with thyroglobulin during its maturation. J. Biol. Chem. (0021-9258) 1994 Kuznetsov G., Chen LB., Nigam SK. No contact email address given. DAVET rat-pccl3 Rattus norvegicus thyroid follicular cell line PCCl3 pccl3 Rat thyroid follicular cell line PCCL3 cells are derived from rat thyroid follicular cells and are dependent on TSH for growth. They also retain differentiated properties as they trap iodide, express thyroglobulin, thyroid peroxidase, and the TSH receptor in a TSH-dependent manner. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kuznetsov G., Chen LB., Nigam SK. No contact email address given. Accepted 2007-Not a month!-12 by DAVET. J. Biol. Chem. (0021-9258) 1994 DAVET Several endoplasmic reticulum stress proteins, including ERp72, interact with thyroglobulin during its maturation. Several endoplasmic reticulum stress proteins, including ERp72, interact with thyroglobulin during its maturation. J. Biol. Chem. (0021-9258) 1994 Kuznetsov G., Chen LB., Nigam SK. No contact email address given. DAVET rat-frtl_5 Rattus norvegicus thyroid cell line FRTL-5 frtl_5 Rat thyroid cell line FRTL-5 This thyroid cell line has been established from normal thyroid glands from 5 to 6-week-old Fischer rats. FRTL-5 is a clonal line of the continuously cultured FRTL cells derived by successive clonal isolation. The cells are characterised by maintaining highly differentiated thyroid features such as secretion of thyroglobulin and concentration of iodide. They display a sensitivity to thyroid-stimulating hormone (TSH)-induced increase in cAMP levels detecting TSH as absolute growth requirement. anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Kuznetsov G., Chen LB., Nigam SK. No contact email address given. Accepted 2007-Not a month!-12 by DAVET. J. Biol. Chem. (0021-9258) 1994 DAVET pdia4_rat Protein disulfide-isomerase A4 Endoplasmic reticulum resident protein 72 Calcium-binding protein 2 Pdia4 Cabp2 Erp70 Endoplasmic reticulum resident protein 70 protein protein rat Rattus norvegicus (Rat) Rat MKLRKAWLLVLLLALTQLLAAASAEDAHEDASDSENPIEDDDDEEEDEEDEDDLEVKEENGVWVLNDENFDNFVADKDTVLLEFYAPWCGHCKQFAPEYEKIASTLKDNDPPIAVAKIDATSASMLASKFDVSGYPTIKILKKGQAVDYDGSRTQEEIVAKVREVSQPDWTPPPEVTLTLTKENFDDVVNNADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATEQTDLAKRFDVSGYPTLKIFRKGRPFDYNGPREKYGIVDYMVEQSGPPSKEILTLKQVQEFLKDGDDVVILGVFQGVGDPGYLQYQDAANTLREDYKFHHTFSTEIAKFLKVSLGKLVLMQPEKFQSKYEPRMHVMDVQGSTEASAIKDYVVKHALPLVGHRKTSNDAKRYSKRPLVVVYYSVDFSFDYRTATQFWRNKVLEVAKDFPEYTFAIADEEDYATEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDALQEFVMAFKKGKLKPVIKSQPVPKNNKGPVRVVVGKTFDAIVMDPKKDVLIEFYAPWCGHCKQLEPVYTSLGKKYKGQKDLVIAKMDATANDITNDRYKVEGFPTIYFAPSGDKKNPIKFEGGNRDLEHLSKFIDEHATKRSRTKEEL C5BC8536834543ED q0r337_rat Grp94 protein protein rat Rattus norvegicus (Rat) Rat MRVLWVLGLCCVLLTFG 2A032499EAA7F077 bip_rat Endoplasmic reticulum chaperone BiP Hspa5 Grp78 Immunoglobulin heavy chain-binding protein Steroidogenesis-activator polypeptide 78 kDa glucose-regulated protein Binding-immunoglobulin protein Heat shock protein 70 family protein 5 Heat shock protein family A member 5 protein protein rat Rattus norvegicus (Rat) Rat MKFTVVAAALLLLCAVRAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSPEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSGGPPPTGEEDTSEKDEL 9D686C6484150108 thyg_rat Thyroglobulin Tg protein protein rat Rattus norvegicus (Rat) Rat MMTLVLWVSTLLSSVCLVAANIFEYQVDAQPLRPCELQREKAFLKQDEYVPQCSEDGSFQTVQCQNDGQSCWCVDSDGTEVPGSRQLGRPTACLSFCQLHKQRILLSSYINSTDALYLPQCQDSGNYAPVQCDLQQVQCWCVDTEGMEVYGTRQQGRPTRCPRSCEIRSRRLLHGVGDKSPPQCDADGEFMPVQCKFVNTTDMMIFDLIHNYNRFPDAFVTFSAFRNRFPEVSGYCYCADSQGRELAETGLELLLDEIYDTIFAGLDQASTFTQSTMYRILQRRFLAIQLVISGRFRCPTKCEVEQFTATSFGHPYIPSCHRDGHYQTVQCQMERMCWCVDAQGIEIPGTRQQGQPLFCAKDQSCASERQQALSRLYFETPGYFSPQDLLSSEDRLVPVSGARLDISCPPRIKELFVDSGLLRSIAVERYQQLSESRSLLREAIRAIFPSRELAGLALQFTTNPKRLQQNLFGGTFLVNAAQLNLSGALGTRSTFNFSQFFQQFGLPGFLVRDRATDLAKLLPVSLDSSPTPVPLRVPEKRVAMNKSVVGTFGFKVNLQENQDALKFLVSLMELPEFLVFLQRAVSVPEDRARDLGDVMEMVFSAQACKQTSGRFFVPSCTAEGSYEDIQCYAGECWCVNSQGKEVEGSRVSGGHPRCPTKCEKQRAQMQNLAGAQPAGSSFFVPTCTSEGYFLPVQCFNSECYCVDAEGQVIPGTQSTIGEPKLCPSVCQLQAEQAFLGVVGVLLSNSSMVPPISSVYIPQCSTSGQWMPVQCDGPHEQVFEWYERWNTQNSDGQELTTATLLMKLMSYREVASTNFSLFLQSLYDAGQQSIFPVLAQYPSLQDVPQVVLEGATIQPGENIFLDPYIFWQILNGQLSQYPGPYSDFSMPLEHFNLRSCWCVDEAGQELDGTRTRAGEIPACPGPCEEVKFRVLKFIKETEEIVSASNASSFPLGESFLVAKGIQLTSEELGLPPLYPSREAFSEKFLRGSEYAIRLAAQSTLTFYQKLRASLGESNGTASLLWSGPYMPQCNTIGGWEPVQCHPGTGQCWCVDGWGELIPGSLMARSSQMPQCPTSCELSRANGLISAWKQAGHQRNPGPGDLFTPVCLQTGEYVRQQTSGTGAWCVDPSSGEGVPTNTNSSAQCPGLCDALKSRVLSRKVGLGYTPVCEALDGGFSPVQCDLAQGSCWCVLASGEEVPGTRVVGTQPACESPQCPLPFSGSDVTDGVVFCETASSSGVTTVQQCQLFCRQGLRNVFSPGPLICNLESQRWVTLPLPRACQRPQLWQTMQTQAHFQLLLPPGKMCSIDYSGLLQAFQVFILDELITRGFCQIQVKTFGTLVSRTVCDNSSIQVGCLTAERLGVNATWKLQLEDISVGSLPNLHSIERALMGQDLLGRFANLIQSGKFQLHLDSKTFSADTILYFLNGDRFVTSPMTQLGCLEGFYRVSTTSQDPLGCVKCPEGSFSQDGKCTPCPAGTYQGQAGSSACIPCPRGRTTTITGAFSKTHCVTDCQRDEAGLQCDQNGQYQANQKDMDSGEVFCVDSEGQRLQWLQTEAGLSESQCLMMRKFEKAPESKVIFDASSPVIVKSRVPSANSPLVQCLADCADDEACSFVTVSSMSSEVSCDLYSWTRDNFACVTSDQEEDAVDSLKETSFGSLRCQVKVRNSGKDSLAVYVKKGHEFTASGQKSFEPTGFQNVLSGLYSSVVFSALGTNLTDTHLFCLLACDQDSCCDGFIVTQVKEGPTICGLLSAPDILVCHINDWRDASDTQANGTCAGVTYDQGSRQMTMSLGGQEFLQGLTLLEGTQDSFISFQQVYLWKDSDIGSRPESMGCGRGMVPKSEAPEGADMATELFSPVDITQVIVNTSHSLPSQQYWLSTHLFSAEQANLWCLSRCAQEPVFCQLADIMESSSLYFTCSLYPEAQVCDNDVESNAKNCSQILPRQPTALFQRKVVLNDRVKNFYTRLPFQKLSGISIRDRIPMSEKLISNGFFECERLCDRDPCCTGFGFLNVSQMQGGEMTCLTLNSMGIQTCSEENGATWRILDCGSEDTEVHTYPFGWYQKPAVWSDAPSFCPSAALQSLTEEKVALDSWQTLALSSVIIDPSIKHFDVAHISISATRNFSLAQDFCLQECSRHQDCLVTTLQIQQGVVRCVFYPDIQSCEHSLRSKTCWLLLHEEAAYIYRKSGAPLHQSDGISTPSVHIDSFGQLQGGSQVVKVGTAWKQVYQFLGVPYAAPPLAENRFQAPEVLNWTGSWDATKLRSSCWQPGTRTPTPPQISEDCLYLNVFVPENLVSNASVLVFFHNTVEMEGSGGQLNIDGSILAAVGNLIVVTANYRLGVFGFLSSGSDEVAGNWGLLDQVAALTWVQTHIGAFGGDPQRVTLAADRGGADVASIHLLITRPTRLQLFRKALLMGGSALSPAAIISPDRAQQQAAALAKEVGCPNSSVQEVVSCFRQKPANILNEAQTKLLAVSGPFHYWGPVVDGQYLRELPSRRLKRPLPVKVDLLIGGSQDDGLINRAKAVKQFEESQGRTNSKTAFYQALQNSLGGEDSDARILAAAIWYYSLEHSTDDYASFSRALENATRDYFIICPIVNMASLWARRTRGNVFMYHVPESYGHGSLELLADVQYAFGLPFYSAYQGYFSTEEQSLSLKVMQYFSNFIRSGNPNYPHEFSQKAAEFATPWPDFVPGAGGESYKELSAQLPNRQGLKKADCSFWSKYIQTLKDADGAKDAQLTKSGEEDLEVGPGSEEDFSGSLEPVPKSYSK 290DD6943FF23F3D tg-hspa5 4 7 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2 Tg antibodies coprecipitated a 75-kDa polypeptide (BiP), and BiP antibodies coprecipitated a 300-kDa polypeptide (Tg) ATP and Mg2+ - Tg did not coimmunoprecipitate BiP when cells were lysed in the presence of 2 mM ATP and 2 mM MgCl2. This suggests that the dissociation of Tg and BiP requires an ATP-hydrolyzing reaction. hspa5-tg 4 7 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2 Tg antibodies coprecipitated a 75-kDa polypeptide (BiP), and BiP antibodies coprecipitated a 300-kDa polypeptide (Tg) ATP and Mg2+ - Tg did not coimmunoprecipitate with BiP when cells were lysed in the presence of 2 mM ATP and 2 mM MgCl2. This suggests that the dissociation of Tg and BiP requires an ATP-hydrolyzing reaction. tg-hspa5-1 2 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait association association 3 This interaction occurred in cells cross-linked using DSP. The amount of ERp72 coimmunoprecipitated with Tg was increased more than 10-fold in the cross-linked cells as compared to untreated cells. tg-hspa5-2 2 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait association association 3 These interactions occurred in uncross-linked cells. In the uncrossed-linked cell lysates grp94 was not coimmunoprecipitated with Tg. hspa5-tg-1 2 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 1 C the same proteins appeared to be coimmunoprecipitated with anti-BiP antiserum (Fig. lA, lanes 5-8). The identities of Tg, This interaction occurred inl ysates from cells cross-linked with DSP and untreated cells. pdia4-tg 3 8 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 4 B This interaction only occurred in lysates from cells cross-linked with DSP. pdia4-grp94 3 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey association association 4 D These interactions only occurred in lysates from cells cross-linked with DSP. grp94-hspa5 1 5 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component physical association physical association 5 The amount of ERp72 cosedimenting with Tg was increased in lysates from DSP cross-linked cells. The amount of grp74 cosedimenting with Tg was markedly increased in lysates from DSP cross-linked cells, and very little was cosedimented in untreated cells. The participant EBI-916036(P06761) was duplicated. It is posssible that originally this interaction did contain more participants pointing to different Trembl proteins which have been merged to a single swissprot protein later.