IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Components of a new human protein kinase signal transduction pathway. Components of a new human protein kinase signal transduction pathway. J. Biol. Chem. (0021-9258) 1995 Zhou G., Bao ZQ., Dixon JE. HeLa cell cDNA in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Zhou G., Bao ZQ., Dixon JE. Accepted 2007-APR-22 by DAVET. HeLa cell cDNA J. Biol. Chem. (0021-9258) 1995 Components of a new human protein kinase signal transduction pathway. Components of a new human protein kinase signal transduction pathway. J. Biol. Chem. (0021-9258) 1995 Zhou G., Bao ZQ., Dixon JE. HeLa cell cDNA in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Zhou G., Bao ZQ., Dixon JE. Accepted 2007-APR-22 by DAVET. HeLa cell cDNA J. Biol. Chem. (0021-9258) 1995 Components of a new human protein kinase signal transduction pathway. Components of a new human protein kinase signal transduction pathway. J. Biol. Chem. (0021-9258) 1995 Zhou G., Bao ZQ., Dixon JE. HeLa cell cDNA yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Zhou G., Bao ZQ., Dixon JE. Accepted 2007-APR-22 by DAVET. HeLa cell cDNA J. Biol. Chem. (0021-9258) 1995 mk07_human Mitogen-activated protein kinase 7 MAPK7 BMK1 ERK5 PRKM7 Extracellular signal-regulated kinase 5 Big MAP kinase 1 protein protein human Homo sapiens Human MAEPLKEEDGEDGSAEPPGPVKAEPAHTAASVAAKNLALLKARSFDVTFDVGDEYEIIETIGNGAYGVVSSARRRLTGQQVAIKKIPNAFDVVTNAKRTLRELKILKHFKHDNIIAIKDILRPTVPYGEFKSVYVVLDLMESDLHQIIHSSQPLTLEHVRYFLYQLLRGLKYMHSAQVIHRDLKPSNLLVNENCELKIGDFGMARGLCTSPAEHQYFMTEYVATRWYRAPELMLSLHEYTQAIDLWSVGCIFGEMLARRQLFPGKNYVHQLQLIMMVLGTPSPAVIQAVGAERVRAYIQSLPPRQPVPWETVYPGADRQALSLLGRMLRFEPSARISAAAALRHPFLAKYHDPDDEPDCAPPFDFAFDREALTRERIKEAIVAEIEDFHARREGIRQQIRFQPSLQPVASEPGCPDVEMPSPWAPSGDCAMESPPPAPPPCPGPAPDTIDLTLQPPPPVSEPAPPKKDGAISDNTKAALKAALLKSLRSRLRDGPSAPLEAPEPRKPVTAQERQREREEKRRRRQERAKEREKRRQERERKERGAGASGGPSTDPLAGLVLSDNDRSLLERWTRMARPAAPALTSVPAPAPAPTPTPTPVQPTSPPPGPVAQPTGPQPQSAGSTSGPVPQPACPPPGPAPHPTGPPGPIPVPAPPQIATSTSLLAAQSLVPPPGLPGSSTPGVLPYFPPGLPPPDAGGAPQSSMSESPDVNLVTQQLSKSQVEDPLPPVFSGTPKGSGAGYGVGFDLEEFLNQSFDMGVADGPQDGQADSASLSASLLADWLEGHGMNPADIESLQREIQMDSPMLLADLPDLQDP 27729FE31658CE3B mp2k5_human Dual specificity mitogen-activated protein kinase kinase 5 MAP2K5 MEK5 MKK5 PRKMK5 MAPK/ERK kinase 5 protein protein human Homo sapiens Human MLWLALGPFPAMENQVLVIRIKIPNSGAVDWTVHSGPQLLFRDVLDVIGQVLPEATTTAFEYEDEDGDRITVRSDEEMKAMLSYYYSTVMEQQVNGQLIEPLQIFPRACKPPGERNIHGLKVNTRAGPSQHSSPAVSDSLPSNSLKKSSAELKKILANGQMNEQDIRYRDTLGHGNGGTVYKAYHVPSGKILAVKVILLDITLELQKQIMSELEILYKCDSSYIIGFYGAFFVENRISICTEFMDGGSLDVYRKMPEHVLGRIAVAVVKGLTYLWSLKILHRDVKPSNMLVNTRGQVKLCDFGVSTQLVNSIAKTYVGTNAYMAPERISGEQYGIHSDVWSLGISFMELALGRFPYPQIQKNQGSLMPLQLLQCIVDEDSPVLPVGEFSEPFVHFITQCMRKQPKERPAPEELMGHPFIVQFNDGNAAVVSMWVCRALEERRSQQGPP F23BB327E2A9C7DC map2k5-mapk7-1 3 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait Q13163:p.[Ser311Ala;Thr315Ala] mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain T A Wed May 02 16:49:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser311ala,thr315ala' Tue Jun 30 17:12:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser311Ala;Thr315Ala]' Q13163:p.Lys195Met mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K M Wed May 02 19:50:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys195met' Tue Jun 30 17:40:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys195Met' physical association physical association It should be noted that wild-type MEK5 also interacted with ERK5, although we did not isolate any ERK5 clones from the initial yeast two-hybrid screen. Fig. 3, A-C Erk5 is an ambiguous identifier, identity taken from sequence in Fig. 4 map2k5-mapk7-2 2 4 unspecified role unspecified role prey prey in vitro In vitro 5 unspecified role unspecified role bait bait Q13163:p.Lys195Met mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K M Wed May 02 16:05:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys195met' Tue Jun 30 20:18:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys195Met' region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association As shown in Fig. 3D, ERK5 binds to GST-MEK5 and the GST-MEK5 mutant K195M, but not to GST alone or to GST-MEK1 Fig. 3D Erk5 is an ambiguous identifier, identity taken from sequence in Fig. 4 mapk7-1 1 4 putative self putative self putative self putative self region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain [EBI-1214040]The shifted ranges (1-432) couldn't be applied as the new full feature sequence is different from the previous one. [EBI-1214040]1-432 [EBI-1214040]Q13164,1 ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true Autophosphorylation of recombinant GST-ERK5 (1-431). Fig. 5 Erk5 is an ambiguous identifier, identity taken from sequence in Fig. 4