IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Acetylation-Dependent Signal Transduction for Type I Interferon Receptor. Acetylation-Dependent Signal Transduction for Type I Interferon Receptor. Cell (0092-8674) 2007 imex curation Tang X., Gao JS., Guan YJ., McLane KE., Yuan ZL., Ramratnam B., Chin YE. HeLa cells treated with or without Interferon alpha for 30 min prior to assay. y_eugene_chin@brown.edu 13-Nov-2007: Contacted by JYOTI. davet Only protein-protein interactions 23 human-u6a STAT2 -/- fibrosarcma line u6a STAT2 -/- human fibrosarcoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Tang X., Gao JS., Guan YJ., McLane KE., Yuan ZL., Ramratnam B., Chin YE. HeLa cells treated with or without Interferon alpha for 30 min prior to assay. y_eugene_chin@brown.edu Accepted 2007-DEC-16 by DAVET. 13-Nov-2007: Contacted by JYOTI. Cell (0092-8674) 2007 davet Only protein-protein interactions 23 imex curation Acetylation-Dependent Signal Transduction for Type I Interferon Receptor. Acetylation-Dependent Signal Transduction for Type I Interferon Receptor. Cell (0092-8674) 2007 imex curation Tang X., Gao JS., Guan YJ., McLane KE., Yuan ZL., Ramratnam B., Chin YE. HeLa cells treated with or without Interferon alpha for 30 min prior to assay. y_eugene_chin@brown.edu 13-Nov-2007: Contacted by JYOTI. davet Only protein-protein interactions 23 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tang X., Gao JS., Guan YJ., McLane KE., Yuan ZL., Ramratnam B., Chin YE. HeLa cells treated with or without Interferon alpha for 30 min prior to assay. y_eugene_chin@brown.edu Accepted 2007-DEC-16 by DAVET. Cell (0092-8674) 2007 davet Only protein-protein interactions 23 imex curation Acetylation-Dependent Signal Transduction for Type I Interferon Receptor. Acetylation-Dependent Signal Transduction for Type I Interferon Receptor. Cell (0092-8674) 2007 imex curation Tang X., Gao JS., Guan YJ., McLane KE., Yuan ZL., Ramratnam B., Chin YE. HeLa cells treated with or without Interferon alpha for 30 min prior to assay. y_eugene_chin@brown.edu 13-Nov-2007: Contacted by JYOTI. davet Only protein-protein interactions 23 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Tang X., Gao JS., Guan YJ., McLane KE., Yuan ZL., Ramratnam B., Chin YE. HeLa cells treated with or without Interferon alpha for 30 min prior to assay. y_eugene_chin@brown.edu Accepted 2007-DEC-16 by DAVET. 13-Nov-2007: Contacted by JYOTI. Cell (0092-8674) 2007 davet Only protein-protein interactions 23 imex curation Acetylation-Dependent Signal Transduction for Type I Interferon Receptor. Acetylation-Dependent Signal Transduction for Type I Interferon Receptor. Cell (0092-8674) 2007 imex curation Tang X., Gao JS., Guan YJ., McLane KE., Yuan ZL., Ramratnam B., Chin YE. HeLa cells treated with or without Interferon alpha for 30 min prior to assay. y_eugene_chin@brown.edu 13-Nov-2007: Contacted by JYOTI. davet Only protein-protein interactions 23 in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Tang X., Gao JS., Guan YJ., McLane KE., Yuan ZL., Ramratnam B., Chin YE. HeLa cells treated with or without Interferon alpha for 30 min prior to assay. y_eugene_chin@brown.edu Accepted 2007-DEC-16 by DAVET. 13-Nov-2007: Contacted by JYOTI. Cell (0092-8674) 2007 davet Only protein-protein interactions 23 imex curation Acetylation-Dependent Signal Transduction for Type I Interferon Receptor. Acetylation-Dependent Signal Transduction for Type I Interferon Receptor. Cell (0092-8674) 2007 imex curation Tang X., Gao JS., Guan YJ., McLane KE., Yuan ZL., Ramratnam B., Chin YE. HeLa cells treated with or without Interferon alpha for 30 min prior to assay. y_eugene_chin@brown.edu 13-Nov-2007: Contacted by JYOTI. davet Only protein-protein interactions 23 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Tang X., Gao JS., Guan YJ., McLane KE., Yuan ZL., Ramratnam B., Chin YE. HeLa cells treated with or without Interferon alpha for 30 min prior to assay. Source of STAT1 and STAT2 identified by residue mapping to known sequences y_eugene_chin@brown.edu Accepted 2007-DEC-16 by DAVET. 13-Nov-2007: Contacted by JYOTI. Cell (0092-8674) 2007 davet Only protein-protein interactions 23 imex curation Acetylation-Dependent Signal Transduction for Type I Interferon Receptor. Acetylation-Dependent Signal Transduction for Type I Interferon Receptor. Cell (0092-8674) 2007 imex curation Tang X., Gao JS., Guan YJ., McLane KE., Yuan ZL., Ramratnam B., Chin YE. HeLa cells treated with or without Interferon alpha for 30 min prior to assay. y_eugene_chin@brown.edu 13-Nov-2007: Contacted by JYOTI. davet Only protein-protein interactions 23 mouse-fibroblast Mus musculus fibroblast Fibroblast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tang X., Gao JS., Guan YJ., McLane KE., Yuan ZL., Ramratnam B., Chin YE. HeLa cells treated with or without Interferon alpha for 30 min prior to assay. y_eugene_chin@brown.edu Accepted 2007-DEC-16 by DAVET. 13-Nov-2007: Contacted by JYOTI. Cell (0092-8674) 2007 davet Only protein-protein interactions 23 imex curation Acetylation-Dependent Signal Transduction for Type I Interferon Receptor. Acetylation-Dependent Signal Transduction for Type I Interferon Receptor. Cell (0092-8674) 2007 imex curation Tang X., Gao JS., Guan YJ., McLane KE., Yuan ZL., Ramratnam B., Chin YE. HeLa cells treated with or without Interferon alpha for 30 min prior to assay. y_eugene_chin@brown.edu 13-Nov-2007: Contacted by JYOTI. davet Only protein-protein interactions 23 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tang X., Gao JS., Guan YJ., McLane KE., Yuan ZL., Ramratnam B., Chin YE. HeLa cells treated with or without Interferon alpha for 30 min prior to assay. y_eugene_chin@brown.edu Accepted 2007-DEC-16 by DAVET. 13-Nov-2007: Contacted by JYOTI. Cell (0092-8674) 2007 davet Only protein-protein interactions 23 imex curation Acetylation-Dependent Signal Transduction for Type I Interferon Receptor. Acetylation-Dependent Signal Transduction for Type I Interferon Receptor. Cell (0092-8674) 2007 imex curation Tang X., Gao JS., Guan YJ., McLane KE., Yuan ZL., Ramratnam B., Chin YE. HeLa cells treated with or without Interferon alpha for 30 min prior to assay. y_eugene_chin@brown.edu 13-Nov-2007: Contacted by JYOTI. davet Only protein-protein interactions 23 mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tang X., Gao JS., Guan YJ., McLane KE., Yuan ZL., Ramratnam B., Chin YE. HeLa cells treated with or without Interferon alpha for 30 min prior to assay. y_eugene_chin@brown.edu Accepted 2007-DEC-16 by DAVET. 13-Nov-2007: Contacted by JYOTI. Cell (0092-8674) 2007 davet Only protein-protein interactions 23 imex curation inar2_human Interferon alpha/beta receptor 2 IFNAR2 IFNARB Type I interferon receptor 2 IFNABR Interferon alpha binding protein protein protein human Homo sapiens Human MLLSQNAFIFRSLNLVLMVYISLVFGISYDSPDYTDESCTFKISLRNFRSILSWELKNHSIVPTHYTLLYTIMSKPEDLKVVKNCANTTRSFCDLTDEWRSTHEAYVTVLEGFSGNTTLFSCSHNFWLAIDMSFEPPEFEIVGFTNHINVMVKFPSIVEEELQFDLSLVIEEQSEGIVKKHKPEIKGNMSGNFTYIIDKLIPNTNYCVSVYLEHSDEQAVIKSPLKCTLLPPGQESESAESAKIGGIITVFLIALVLTSTIVTLKWIGYICLRNSLPKVLNFHNFLAWPFPNLPPLEAMDMVEVIYINRKKKVWDYNYDDESDSDTEAAPRTSGGGYTMHGLTVRPLGQASATSTESQLIDPESEEEPDLPEVDVELPTMPKDSPQQLELLSGPCERRKSPLQDPFPEEDYSSTEGSGGRITFNVDLNSVFLRVLDDEDSDDLEAPLMLSSHLEEMVDPEDPDNVQSNHLLASGEGTQPTFPSPSSEGLWSEDAPSDQSDTSESDVDLGDGYIMR 4D7730D93AA739F4 ep300_human Histone acetyltransferase p300 Histone butyryltransferase p300 Histone crotonyltransferase p300 Protein propionyltransferase p300 EP300 P300 E1A-associated protein p300 Protein 2-hydroxyisobutyryltransferase p300 Protein lactyltransferas p300 protein protein human Homo sapiens Human MAENVVEPGPPSAKRPKLSSPALSASASDGTDFGSLFDLEHDLPDELINSTELGLTNGGDINQLQTSLGMVQDAASKHKQLSELLRSGSSPNLNMGVGGPGQVMASQAQQSSPGLGLINSMVKSPMTQAGLTSPNMGMGTSGPNQGPTQSTGMMNSPVNQPAMGMNTGMNAGMNPGMLAAGNGQGIMPNQVMNGSIGAGRGRQNMQYPNPGMGSAGNLLTEPLQQGSPQMGGQTGLRGPQPLKMGMMNNPNPYGSPYTQNPGQQIGASGLGLQIQTKTVLSNNLSPFAMDKKAVPGGGMPNMGQQPAPQVQQPGLVTPVAQGMGSGAHTADPEKRKLIQQQLVLLLHAHKCQRREQANGEVRQCNLPHCRTMKNVLNHMTHCQSGKSCQVAHCASSRQIISHWKNCTRHDCPVCLPLKNAGDKRNQQPILTGAPVGLGNPSSLGVGQQSAPNLSTVSQIDPSSIERAYAALGLPYQVNQMPTQPQVQAKNQQNQQPGQSPQGMRPMSNMSASPMGVNGGVGVQTPSLLSDSMLHSAINSQNPMMSENASVPSLGPMPTAAQPSTTGIRKQWHEDITQDLRNHLVHKLVQAIFPTPDPAALKDRRMENLVAYARKVEGDMYESANNRAEYYHLLAEKIYKIQKELEEKRRTRLQKQNMLPNAAGMVPVSMNPGPNMGQPQPGMTSNGPLPDPSMIRGSVPNQMMPRITPQSGLNQFGQMSMAQPPIVPRQTPPLQHHGQLAQPGALNPPMGYGPRMQQPSNQGQFLPQTQFPSQGMNVTNIPLAPSSGQAPVSQAQMSSSSCPVNSPIMPPGSQGSHIHCPQLPQPALHQNSPSPVPSRTPTPHHTPPSIGAQQPPATTIPAPVPTPPAMPPGPQSQALHPPPRQTPTPPTTQLPQQVQPSLPAAPSADQPQQQPRSQQSTAASVPTPTAPLLPPQPATPLSQPAVSIEGQVSNPPSTSSTEVNSQAIAEKQPSQEVKMEAKMEVDQPEPADTQPEDISESKVEDCKMESTETEERSTELKTEIKEEEDQPSTSATQSSPAPGQSKKKIFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDIVKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQEIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQGESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAGFVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHASDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPPPNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDYIFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFEGDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLSRGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDLMDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQDRFVYTCNECKHHVETRWHCTVCEDYDLCITCYNTKNHDHKMEKLGLGLDDESNNQQAAATQSPGDSRRLSIQRCIQSLVHACQCRNANCSLPSCQKMKRVVQHTKGCKRKTNGGCPICKQLIALCCYHAKHCQENKCPVPFCLNIKQKLRQQQLQHRLQQAQMLRRRMASMQRTGVVGQQQGLPSPTPATPTTPTGQQPTTPQTPQPTSQPQPTPPNSMPPYLPRTQAAGPVSQGKAAGQVTPPTPPQTAQPPLPGPPPAAVEMAMQIQRAAETQRQMAHVQIFQRPIQHQMPPMTPMAPMGMNPPPMTRGPSGHLEPGMGPTGMQQQPPWSQGGLPQPQQLQSGMPRPAMMSVAQHGQPLNMAPQPGLGQVGISPLKPGTVSQQALQNLLRTLRSPSSPLQQQQVLSILHANPQLLAAFIKQRAAKYANSNPQPIPGQPGMPQGQPGLQPPTMPGQQGVHSNPAMQNMNPMQAGVQRAGLPQQQPQQQLQPPMGGMSPQAQQMNMNHNTMPSQFRDILRRQQMMQQQQQQGAGPGIGPGMANHNQFQQPQGVGYPPQQQQRMQHHMQQMQQGNMGQIGQLPQALGAEAGASLQAYQQRLLQQQMGSPVQPNPMSPQQHMLPNQAQSPHLQGQQIPNSLSNQVRSPQPVPSPRPQSQPPHSSPSPRMQPQPSPHHVSPQTSSPHPGLVAAQANPMEQGHFASPDQNSMLSQLASNPGMANLHGASATDLGLSTDNSDLNSNLSQSTLDIH DIP protein Q09472 original sequence version: 193 DIP protein Q09472 original sequence version: 186 DIP protein Q09472 original sequence version: 107 DIP protein Q09472 original sequence version: 112 DIP protein Q09472 original sequence version: 181 DIP protein Q09472 original sequence version: 183 DIP protein Q09472 original sequence version: 136 DIP protein Q09472 original sequence version: 146 DIP protein Q09472 original sequence version: 165 DIP protein Q09472 original sequence version: 192 DIP protein Q09472 original sequence version: 200 DIP protein Q09472 original sequence version: 206 DIP protein Q09472 original sequence version: 194 DIP protein Q09472 original sequence version: 171 DIP protein Q09472 original sequence version: 142 DIP protein Q09472 original sequence version: 180 8E869E1F174A6FEB irf9_mouse Interferon regulatory factor 9 Isgf3g Transcriptional regulator ISGF3 subunit gamma IFN-alpha-responsive transcription factor subunit Interferon-stimulated gene factor 3 gamma ISGF3 p48 subunit Irf9 protein protein mouse Mus musculus Mouse MASGKVRCTRKLRSWIVEQVESGHFPGVCWDDAAKTMFRIPWKHAGKQDFREDQDAAIFKAWALFKEKHKDGDIGHPAVWKTRLRCALNKSSEFEEVPERGRMDVAEPYKVYRILPAGTLPNQPRNQKSPCKRSISCVSPEREENMENGRTNGVVNHSDSGSNIGGGGNGSNRSDSNSNCNSELEEGAGTTEATIREDPVFLEHQLPLNSDYSLLLTFIYGGRVVGKTQVHSLDCRLVAERSDSESSMEQVEFPKPDPLEPTQHLLNQLDRGVLVASNSRGLFVQRLCPIPISWNAPEAPPGPGPHLLPSNKCVELFKTTYFCRDLAQYFQGQGPPPKFQATLHFWEESPGSSHSQENLITVQMEQAFARHLLEKIPEEEKAALFLLQHTEQSPSALGH 92F9481ACEB669BD inar2_mouse Interferon alpha/beta receptor 2 Type I interferon receptor 2 Ifnar2 protein protein mouse Mus musculus Mouse MRSRCTVSAVGLLSLCLVVSASLETITPSAFDGYPDEPCTINITIRNSRLILSWELENKSGPPANYTLWYTVMSKDENLTKVKNCSDTTKSSCDVTDKWLEGMESYVVAIVIVHRGDLTVCRCSDYIVPANAPLEPPEFEIVGFTDHINVTMEFPPVTSKIIQEKMKTTPFVIKEQIGDSVRKKHEPKVNNVTGNFTFVLRDLLPKTNYCVSLYFDDDPAIKSPLKCIVLQPGQESGLSESAIVGITTSCLVVMVFVSTIVMLKRIGYICLKDNLPNVLNFRHFLTWIIPERSPSEAIDRLEIIPTNKKKRLWNYDYEDGSDSDEEVPTASVTGYTMHGLTGKPLQQTSDTSASPEDPLHEEDSGAEESDEAGAGAGAEPELPTEAGAGPSEDPTGPYERRKSVLEDSFPREDNSSMDEPGDNIIFNVNLNSVFLRVLHDEDASETLSLEEDTILLDEGPQRTESDLRIAGGDRTQPPLPSLPSQDLWTEDGSSEKSDTSDSDADVGDGYIMR 7A645DA5F33C9EA0 irf9_human Interferon regulatory factor 9 Transcriptional regulator ISGF3 subunit gamma IFN-alpha-responsive transcription factor subunit Interferon-stimulated gene factor 3 gamma ISGF3 p48 subunit IRF9 ISGF3G protein protein human Homo sapiens Human MASGRARCTRKLRNWVVEQVESGQFPGVCWDDTAKTMFRIPWKHAGKQDFREDQDAAFFKAWAIFKGKYKEGDTGGPAVWKTRLRCALNKSSEFKEVPERGRMDVAEPYKVYQLLPPGIVSGQPGTQKVPSKRQHSSVSSERKEEEDAMQNCTLSPSVLQDSLNNEEEGASGGAVHSDIGSSSSSSSPEPQEVTDTTEAPFQGDQRSLEFLLPPEPDYSLLLTFIYNGRVVGEAQVQSLDCRLVAEPSGSESSMEQVLFPKPGPLEPTQRLLSQLERGILVASNPRGLFVQRLCPIPISWNAPQAPPGPGPHLLPSNECVELFRTAYFCRDLVRYFQGLGPPPKFQVTLNFWEESHGSSHTPQNLITVKMEQAFARYLLEQTPEQQAAILSLV F8E3784354BFD4A9 stat1_human Signal transducer and activator of transcription 1-alpha/beta STAT1 Transcription factor ISGF-3 components p91/p84 protein protein human Homo sapiens Human MSQWYELQQLDSKFLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAANDVSFATIRFHDLLSQLDDQYSRFSLENNFLLQHNIRKSKRNLQDNFQEDPIQMSMIIYSCLKEERKILENAQRFNQAQSGNIQSTVMLDKQKELDSKVRNVKDKVMCIEHEIKSLEDLQDEYDFKCKTLQNREHETNGVAKSDQKQEQLLLKKMYLMLDNKRKEVVHKIIELLNVTELTQNALINDELVEWKRRQQSACIGGPPNACLDQLQNWFTIVAESLQQVRQQLKKLEELEQKYTYEHDPITKNKQVLWDRTFSLFQQLIQSSFVVERQPCMPTHPQRPLVLKTGVQFTVKLRLLVKLQELNYNLKVKVLFDKDVNERNTVKGFRKFNILGTHTKVMNMEESTNGSLAAEFRHLQLKEQKNAGTRTNEGPLIVTEELHSLSFETQLCQPGLVIDLETTSLPVVVISNVSQLPSGWASILWYNMLVAEPRNLSFFLTPPCARWAQLSEVLSWQFSSVTKRGLNVDQLNMLGEKLLGPNASPDGLIPWTRFCKENINDKNFPFWLWIESILELIKKHLLPLWNDGCIMGFISKERERALLKDQQPGTFLLRFSESSREGAITFTWVERSQNGGEPDFHAVEPYTKKELSAVTFPDIIRNYKVMAAENIPENPLKYLYPNIDKDHAFGKYYSRPKEAPEPMELDGPKGTGYIKTELISVSEVHPSRLQTTDNLLPMSPEEFDEVSRIVGSVEFDSMMNTV 054A813522364BA6 cbp_human CREB-binding protein CREBBP CBP Histone lysine acetyltransferase CREBBP Protein-lysine acetyltransferase CREBBP protein protein human Homo sapiens Human MAENLLDGPPNPKRAKLSSPGFSANDSTDFGSLFDLENDLPDELIPNGGELGLLNSGNLVPDAASKHKQLSELLRGGSGSSINPGIGNVSASSPVQQGLGGQAQGQPNSANMASLSAMGKSPLSQGDSSAPSLPKQAASTSGPTPAASQALNPQAQKQVGLATSSPATSQTGPGICMNANFNQTHPGLLNSNSGHSLINQASQGQAQVMNGSLGAAGRGRGAGMPYPTPAMQGASSSVLAETLTQVSPQMTGHAGLNTAQAGGMAKMGITGNTSPFGQPFSQAGGQPMGATGVNPQLASKQSMVNSLPTFPTDIKNTSVTNVPNMSQMQTSVGIVPTQAIATGPTADPEKRKLIQQQLVLLLHAHKCQRREQANGEVRACSLPHCRTMKNVLNHMTHCQAGKACQVAHCASSRQIISHWKNCTRHDCPVCLPLKNASDKRNQQTILGSPASGIQNTIGSVGTGQQNATSLSNPNPIDPSSMQRAYAALGLPYMNQPQTQLQPQVPGQQPAQPQTHQQMRTLNPLGNNPMNIPAGGITTDQQPPNLISESALPTSLGATNPLMNDGSNSGNIGTLSTIPTAAPPSSTGVRKGWHEHVTQDLRSHLVHKLVQAIFPTPDPAALKDRRMENLVAYAKKVEGDMYESANSRDEYYHLLAEKIYKIQKELEEKRRSRLHKQGILGNQPALPAPGAQPPVIPQAQPVRPPNGPLSLPVNRMQVSQGMNSFNPMSLGNVQLPQAPMGPRAASPMNHSVQMNSMGSVPGMAISPSRMPQPPNMMGAHTNNMMAQAPAQSQFLPQNQFPSSSGAMSVGMGQPPAQTGVSQGQVPGAALPNPLNMLGPQASQLPCPPVTQSPLHPTPPPASTAAGMPSLQHTTPPGMTPPQPAAPTQPSTPVSSSGQTPTPTPGSVPSATQTQSTPTVQAAAQAQVTPQPQTPVQPPSVATPQSSQQQPTPVHAQPPGTPLSQAAASIDNRVPTPSSVASAETNSQQPGPDVPVLEMKTETQAEDTEPDPGESKGEPRSEMMEEDLQGASQVKEETDIAEQKSEPMEVDEKKPEVKVEVKEEEESSSNGTASQSTSPSQPRKKIFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDIVKNPMDLSTIKRKLDTGQYQEPWQYVDDVWLMFNNAWLYNRKTSRVYKFCSKLAEVFEQEIDPVMQSLGYCCGRKYEFSPQTLCCYGKQLCTIPRDAAYYSYQNRYHFCEKCFTEIQGENVTLGDDPSQPQTTISKDQFEKKKNDTLDPEPFVDCKECGRKMHQICVLHYDIIWPSGFVCDNCLKKTGRPRKENKFSAKRLQTTRLGNHLEDRVNKFLRRQNHPEAGEVFVRVVASSDKTVEVKPGMKSRFVDSGEMSESFPYRTKALFAFEEIDGVDVCFFGMHVQEYGSDCPPPNTRRVYISYLDSIHFFRPRCLRTAVYHEILIGYLEYVKKLGYVTGHIWACPPSEGDDYIFHCHPPDQKIPKPKRLQEWYKKMLDKAFAERIIHDYKDIFKQATEDRLTSAKELPYFEGDFWPNVLEESIKELEQEEEERKKEESTAASETTEGSQGDSKNAKKKNNKKTNKNKSSISRANKKKPSMPNVSNDLSQKLYATMEKHKEVFFVIHLHAGPVINTLPPIVDPDPLLSCDLMDGRDAFLTLARDKHWEFSSLRRSKWSTLCMLVELHTQGQDRFVYTCNECKHHVETRWHCTVCEDYDLCINCYNTKSHAHKMVKWGLGLDDEGSSQGEPQSKSPQESRRLSIQRCIQSLVHACQCRNANCSLPSCQKMKRVVQHTKGCKRKTNGGCPVCKQLIALCCYHAKHCQENKCPVPFCLNIKHKLRQQQIQHRLQQAQLMRRRMATMNTRNVPQQSLPSPTSAPPGTPTQQPSTPQTPQPPAQPQPSPVSMSPAGFPSVARTQPPTTVSTGKPTSQVPAPPPPAQPPPAAVEAARQIEREAQQQQHLYRVNINNSMPPGRTGMGTPGSQMAPVSLNVPRPNQVSGPVMPSMPPGQWQQAPLPQQQPMPGLPRPVISMQAQAAVAGPRMPSVQPPRSISPSALQDLLRTLKSPSSPQQQQQVLNILKSNPQLMAAFIKQRTAKYVANQPGMQPQPGLQSQPGMQPQPGMHQQPSLQNLNAMQAGVPRPGVPPQQQAMGGLNPQGQALNIMNPGHNPNMASMNPQYREMLRRQLLQQQQQQQQQQQQQQQQQQGSAGMAGGMAGHGQFQQPQGPGGYPPAMQQQQRMQQHLPLQGSSMGQMAAQMGQLGQMGQPGLGADSTPNIQQALQQRILQQQQMKQQIGSPGQPNPMSPQQHMLSGQPQASHLPGQQIATSLSNQVRSPAPVQSPRPQSQPPHSSPSPRIQPQPSPHHVSPQTGSPHPGLAVTMASSIDQGHLGNPEQSAMLPQLNTPSRSALSSELSLVGDTTGDTLEKFVEGL DIP protein Q92793 original sequence version: 134 DIP protein Q92793-1 original sequence version: 171 DIP protein Q92793 original sequence version: 171 DIP protein Q92793 original sequence version: 185 DIP protein Q92793 original sequence version: 106 DIP protein Q92793 original sequence version: 104 DIP protein Q92793 original sequence version: 112 DIP protein Q92793 original sequence version: 135 DIP protein Q92793 original sequence version: 139 DIP protein Q92793 original sequence version: 157 DIP protein Q92793 original sequence version: 177 DIP protein Q92793 original sequence version: 168 DIP protein Q92793-1 original sequence version: 174 DIP protein Q92793 original sequence version: 146 DIP protein Q92793 original sequence version: 161 DIP protein Q92793 original sequence version: 172 3BEA9B8558BA1A5E stat2_human Signal transducer and activator of transcription 2 p113 STAT2 protein protein human Homo sapiens Human MAQWEMLQNLDSPFQDQLHQLYSHSLLPVDIRQYLAVWIEDQNWQEAALGSDDSKATMLFFHFLDQLNYECGRCSQDPESLLLQHNLRKFCRDIQPFSQDPTQLAEMIFNLLLEEKRILIQAQRAQLEQGEPVLETPVESQQHEIESRILDLRAMMEKLVKSISQLKDQQDVFCFRYKIQAKGKTPSLDPHQTKEQKILQETLNELDKRRKEVLDASKALLGRLTTLIELLLPKLEEWKAQQQKACIRAPIDHGLEQLETWFTAGAKLLFHLRQLLKELKGLSCLVSYQDDPLTKGVDLRNAQVTELLQRLLHRAFVVETQPCMPQTPHRPLILKTGSKFTVRTRLLVRLQEGNESLTVEVSIDRNPPQLQGFRKFNILTSNQKTLTPEKGQSQGLIWDFGYLTLVEQRSGGSGKGSNKGPLGVTEELHIISFTVKYTYQGLKQELKTDTLPVVIISNMNQLSIAWASVLWFNLLSPNLQNQQFFSNPPKAPWSLLGPALSWQFSSYVGRGLNSDQLSMLRNKLFGQNCRTEDPLLSWADFTKRESPPGKLPFWTWLDKILELVHDHLKDLWNDGRIMGFVSRSQERRLLKKTMSGTFLLRFSESSEGGITCSWVEHQDDDKVLIYSVQPYTKEVLQSLPLTEIIRHYQLLTEENIPENPLRFLYPRIPRDEAFGCYYQEKVNLQERRKYLKHRLIVVSNRQVDELQQPLELKPEPELESLELELGLVPEPELSLDLEPLLKAGLDLGPELESVLESTLEPVIEPTLCMVSQTVPEPDQGPVSQPVPEPDLPCDLRHLNTEPMEIFRNCVKIEEIMPNGDPLLAGQNTVDEVYVSRPSHFYTDGPLMPSDF DIP protein P52630 original sequence version: 116 E4C74674CB7A3215 stat3_human Signal transducer and activator of transcription 3 Acute-phase response factor STAT3 APRF protein protein human Homo sapiens Human MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATSPM 6C00632211C8012D DIP protein P40763 original sequence version: 157 DIP protein P40763-1 original sequence version: 147 DIP protein P40763-1 original sequence version: 135 DIP protein P40763-1 original sequence version: 157 DIP protein P40763 original sequence version: 165 DIP protein P40763 original sequence version: 182 DIP protein P40763 original sequence version: 135 6C00632211C8012D inar1_human Interferon alpha/beta receptor 1 IFNAR1 IFNAR Type I interferon receptor 1 Cytokine receptor family 2 member 1 Cytokine receptor class-II member 1 protein protein human Homo sapiens Human MMVVLLGATTLVLVAVAPWVLSAAAGGKNLKSPQKVEVDIIDDNFILRWNRSDESVGNVTFSFDYQKTGMDNWIKLSGCQNITSTKCNFSSLKLNVYEEIKLRIRAEKENTSSWYEVDSFTPFRKAQIGPPEVHLEAEDKAIVIHISPGTKDSVMWALDGLSFTYSLVIWKNSSGVEERIENIYSRHKIYKLSPETTYCLKVKAALLTSWKIGVYSPVHCIKTTVENELPPPENIEVSVQNQNYVLKWDYTYANMTFQVQWLHAFLKRNPGNHLYKWKQIPDCENVKTTQCVFPQNVFQKGIYLLRVQASDGNNTSFWSEEIKFDTEIQAFLLPPVFNIRSLSDSFHIYIGAPKQSGNTPVIQDYPLIYEIIFWENTSNAERKIIEKKTDVTVPNLKPLTVYCVKARAHTMDEKLNKSSVFSDAVCEKTKPGNTSKIWLIVGICIALFALPFVIYAAKVFLRCINYVFFPSLKPSSSIDEYFSEQPLKNLLLSTSEEQIEKCFIIENISTIATVEETNQTDEDHKKYSSQTSQDSGNYSNEDESESKTSEELQQDFV 24A01779DB7F356F ifnar2-crebbp-1 2 13 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait association association In HeLa cells or mouse fibroblasts, CBP was associated with IFNalphaR2, and this association was enhanced upon IFNalpha treatment (Figures 1C and S1B). Similarly, IRF9 and IFNalphaR2 also coprecipitated (Figure 1C). Figure 1C IFNalpha - addition enhanced interaction ifnar2-crebbp-2 3 9 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association The interaction between CBP and IFNalphaR2 detected in HeLa cells was confirmed by recovering IFNalphaR2 from anti-CBP precipitates prepared from 293T cells transiently transfected with HA-tagged IFNalphaR2 and HA-tagged CBP (Figure 1D) Figure 1D ifnar2-crebbp-3 5 15 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P48551:p.Ser364Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain S A Wed May 02 18:06:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser364ala' Tue Jun 30 18:14:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser364Ala' P48551:p.Ser384Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 19:23:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser384ala' Tue Jun 30 16:43:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser384Ala' physical association physical association In 293T cells, the Myc-IFNalphaR2 C-terminal truncation mutants were cotransfected with HA-CBP for an association analysis. Figure 1F, 1G ifnar2-stat2-1 5 16 unspecified role unspecified role bait bait n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Anti-Myc precipitates, prepared from 293T cells cotransfected with IFNalphaR2 and STAT2 or IRF9, were probed with anti-HA (IFNalphaR2) or anti-Myc (IRF9 and STAT2). Fig. 3A ifnar2-isgf3g-1 5 13 unspecified role unspecified role bait bait n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Anti-Myc precipitates, prepared from 293T cells cotransfected with IFNalphaR2 and STAT2 or IRF9, were probed with anti-HA (IFNalphaR2) or anti-Myc (IRF9 and STAT2). Fig. 3A ifnar2-isgf3g-2 3 13 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Myc-tagged forms of IFNalphaR2 C-terminal region as indicated were cotransfected with IRF9 Fig. 3B ifnar2-stat2-2 3 16 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association After further truncation up to Ser392 (265�392), the interaction with IRF9 was disrupted (Figure 3B, left panel) without apparently affecting its interaction with STAT2 (Figure 3B, right panel). Fig. 3B ifnar2-isgf3g 7 13 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait P48551:p.Lys399Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:03:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys399ala' Tue Jun 30 16:23:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys399Ala' P48551:p.Ser400Ala mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 14:57:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser400ala' Tue Jun 30 20:09:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser400Ala' P48551:p.Lys399_Ser400delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain KS AA Thu Jul 07 12:07:00 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was lys399ala_ser400ala Fri Jul 22 10:55:10 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'lys399ala,ser400ala' Wed May 02 15:17:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys_ser399-400ala_ala' Tue Jun 30 16:16:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys399_Ser400delinsAlaAla' physical association physical association When both K399R and S400A were introduced, however, the activity of IFNalphaR2 in IRF9 interaction was severely affected (Figure 3C). Figure 3C, S2A ifnar2-isgf3g-3 4 13 unspecified role unspecified role bait bait n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ser-400 (possible) opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain lys-399 (required) naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain in vitro In vitro physical association physical association Fig. 3D ifnar2-isgf3g-4 8 11 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey physical association physical association Consistently, IFNalpha failed to induce the interaction between IFNalphaR2 and IRF9 (Figure 3E) or IRF9 nuclear translocation (data not shown) in CBP-/- mouse embryonic fibroblast (MEF). Fig. 3E Interaction was not observed in CBP-/- cells - this was shown to be due to CBP being responsible for IFNalphaR2 acetylation ifnar2-isgf3g-5 3 13 unspecified role unspecified role prey prey n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q00978:p.Arg292Gln mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R Q Wed May 02 19:54:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg292gln' Tue Jun 30 16:00:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg292Gln' 9 unspecified role unspecified role bait bait physical association physical association IH2D (257�338) of IRF9 is responsible for IFNalphaR2 binding. Fig. 4C & 4E isgf3g-stat2-3 5 13 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 16 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 4D IH1D (339-393) of IRF9 interacts with STAT2. isgf3g-isgf3g-1 3 13 unspecified role unspecified role bait bait c-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role prey prey n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q00978:p.Lys81Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K R Wed May 02 15:44:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys81arg' Tue Jun 30 19:13:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys81Arg' 86 89 physical association physical association Coimmunoprecipitation results revealed that IRF-DBD but not IRF9-DBD with K81R substitution associated with IRF9 either full-length or the C-terminal 118�393 fragment Fig. 5F stat1-stat2-1 3 14 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range dbd region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association STAT2 DBD interacts with STAT1. Fig. 6E stat1-stat2-2 7 14 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey P52630:p.Lys384Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K R Wed May 02 16:45:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys384arg' Tue Jun 30 20:11:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys384Arg' P52630:p.Lys390Arg mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain K R Wed May 02 20:16:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys390arg' Tue Jun 30 18:36:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys390Arg' P52630:p.Lys415Arg mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K R Wed May 02 15:44:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys415arg' Tue Jun 30 17:07:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys415Arg' P52630:p.Lys419Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K R Wed May 02 19:03:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys419arg' Tue Jun 30 18:38:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys419Arg' physical association physical association While STAT2 with a K384R or K419R substitution moderately weakened the STAT2 and STAT1 interactions, STAT2-K390R and to a lesser extent STAT2-K415R interacted more strongly with STAT1 (Figure 6F, left panel). Fig. 6F, 6G isgf3g-stat2-1 7 13 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey physical association physical association In contrast, STAT2 DBD acetylation seemed to be irrelevant to STAT2's interaction with IRF9 (Figure 6F, right panel) Figure 6F stat1-stat2-3 2 14 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey physical association physical association Indeed, we repeatedly detected a stable STAT1:STAT2 interaction, which was slightly diminished following IFNalpha treatment in HeLa cells (Figure 6H). Figure 6H isgf3g-stat2-2 1 13 unspecified role unspecified role prey prey n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 14 unspecified role unspecified role bait bait n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 16 unspecified role unspecified role prey prey n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association To estimate STAT2-K390R mutant on ISGF3 complex formation, Myc-tagged forms of IRF9 and STAT1 were cotransfected with Myc-tagged STAT2 wild-type or K390R mutant in STAT2 null U6A cells. Figure 6I K390R mutated STAT2 shows increased binding to STAT1 but decreased binding to ISGF3G ifnar2-crebbp-4 6 15 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association In HeLa cells or mouse fibroblasts, CBP was associated with IFNalphaR2, and this association was enhanced upon IFNalpha treatment (Figures 1C and S1B). Figure S1B IFNalpha - addition enhanced interaction and results in nuclear transfer It is worthwhilevto note that the CBP and IFNaR2 coprecipitation was only detected in the cytoplasmic fraction, suggesting that IFNaR2 recruits CBP from the cytoplasm rather than from the nucleus ifnar2-ep300-1 2 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association In addition, both IFNalphaR2 and IFNalphaR1 were able to recruit p300 (Figures S1C and S1D). Figure S1C ifnar1-ep300-1 2 10 unspecified role unspecified role prey prey 18 unspecified role unspecified role bait bait physical association physical association In addition, both IFNalphaR2 and IFNalphaR1 were able to recruit p300 (Figures S1C and S1D). Figure S1C isgf3g-stat2-4 3 13 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey n-teminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association this domain of STAT2 (1�314) and IRF9 coprecipitated Fig. S3B, S3C stat2-stat3-1 7 17 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait physical association physical association STAT3, which can also form heterodimer with STAT2 (Figure S3E). Fig. S3E