IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Antagonistic Regulation of PIN Phosphorylation by PP2A and PINOID Directs Auxin Flux. Antagonistic Regulation of PIN Phosphorylation by PP2A and PINOID Directs Auxin Flux. Cell (0092-8674) 2007 imex curation Michniewicz M., Zago MK., Abas L., Weijers D., Schweighofer A., Meskiene I., Heisler MG., Ohno C., Zhang J., Huang F., Schwab R., Weigel D., Meyerowitz EM., Luschnig C., Offringa R., Friml J. Transgenic Arabidopsis plants expressing epitope tagged protein constructs were used in this study. jifri@psb.ugent.be 14-May-2008: Contacted by JYOTI. Only protein-protein interactions 4 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Michniewicz M., Zago MK., Abas L., Weijers D., Schweighofer A., Meskiene I., Heisler MG., Ohno C., Zhang J., Huang F., Schwab R., Weigel D., Meyerowitz EM., Luschnig C., Offringa R., Friml J. Transgenic Arabidopsis plants expressing epitope tagged protein constructs were used in this study. jifri@psb.ugent.be Accepted 2008-APR-7 by CDEROW. 14-May-2008: Contacted by JYOTI. Cell (0092-8674) 2007 Only protein-protein interactions 4 imex curation Antagonistic Regulation of PIN Phosphorylation by PP2A and PINOID Directs Auxin Flux. Antagonistic Regulation of PIN Phosphorylation by PP2A and PINOID Directs Auxin Flux. Cell (0092-8674) 2007 imex curation Michniewicz M., Zago MK., Abas L., Weijers D., Schweighofer A., Meskiene I., Heisler MG., Ohno C., Zhang J., Huang F., Schwab R., Weigel D., Meyerowitz EM., Luschnig C., Offringa R., Friml J. Transgenic Arabidopsis plants expressing epitope tagged protein constructs were used in this study. jifri@psb.ugent.be 14-May-2008: Contacted by JYOTI. Only protein-protein interactions 4 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Michniewicz M., Zago MK., Abas L., Weijers D., Schweighofer A., Meskiene I., Heisler MG., Ohno C., Zhang J., Huang F., Schwab R., Weigel D., Meyerowitz EM., Luschnig C., Offringa R., Friml J. Transgenic Arabidopsis plants expressing epitope tagged protein constructs were used in this study. The myelin basic protein was purchased from Sigma and the source organism is not stated. As this could be one of a number of source organisms avaliable this protein was not annotated in this entry. jifri@psb.ugent.be Accepted 2008-APR-7 by CDEROW. 14-May-2008: Contacted by JYOTI. Cell (0092-8674) 2007 Only protein-protein interactions 4 imex curation 2aaa_arath Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform PP2AA1 EER1 RCN1 REGA F2J7.19 At1g25490 Protein enhancer of ethylene-response 1 Protein ROOTS CURL IN NAPHTHYLPHTHALAMIC ACID 1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAMVDEPLYPIAVLIDELKNDDIQLRLNSIRRLSTIARALGEERTRKELIPFLSENSDDDDEVLLAMAEELGVFIPFVGGIEFAHVLLPPLESLCTVEETCVREKAVESLCKIGSQMKENDLVESFVPLVKRLAGGEWFAARVSACGIFHVAYQGCTDVLKTELRATYSQLCKDDMPMVRRAAASNLGKFATTVESTFLIAEIMTMFDDLTKDDQDSVRLLAVEGCAALGKLLEPQDCVARILPVIVNFSQDKSWRVRYMVANQLYELCEAVGPDCTRTDLVPAYVRLLRDNEAEVRIAAAGKVTKFCRLLNPELAIQHILPCVKELSSDSSQHVRSALASVIMGMAPILGKDSTIEHLLPIFLSLLKDEFPDVRLNIISKLDQVNQVIGIDLLSQSLLPAIVELAEDRHWRVRLAIIEYVPLLASQLGIGFFDDKLGALCMQWLQDKVYSIREAAANNLKRLAEEFGPEWAMQHLVPQVLDMVNNPHYLHRMMVLRAISLMAPVMGSEITCSKFLPVVVEASKDRVPNIKFNVAKLLQSLIPIVDQSVVDKTIRQCLVDLSEDPDVDVRYFANQALNSIDGSTAAQS F27E294E85B8DAFA pin2_arath Auxin efflux carrier component 2 Auxin efflux carrier AGR Polar-auxin-transport efflux component AGR1 Protein AGRAVITROPIC 1 Ethylene-insensitive root 1 Protein WAVY 6 PIN2 AGR AGR1 EIR1 WAV6 MUL3.3 At5g57090 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MITGKDMYDVLAAMVPLYVAMILAYGSVRWWGIFTPDQCSGINRFVAVFAVPLLSFHFISSNDPYAMNYHFLAADSLQKVVILAALFLWQAFSRRGSLEWMITLFSLSTLPNTLVMGIPLLRAMYGDFSGNLMVQIVVLQSIIWYTLMLFLFEFRGAKLLISEQFPETAGSITSFRVDSDVISLNGREPLQTDAEIGDDGKLHVVVRRSSAASSMISSFNKSHGGGLNSSMITPRASNLTGVEIYSVQSSREPTPRASSFNQTDFYAMFNASKAPSPRHGYTNSYGGAGAGPGGDVYSLQSSKGVTPRTSNFDEEVMKTAKKAGRGGRSMSGELYNNNSVPSYPPPNPMFTGSTSGASGVKKKESGGGGSGGGVGVGGQNKEMNMFVWSSSASPVSEANAKNAMTRGSSTDVSTDPKVSIPPHDNLATKAMQNLIENMSPGRKGHVEMDQDGNNGGKSPYMGKKGSDVEDGGPGPRKQQMPPASVMTRLILIMVWRKLIRNPNTYSSLFGLAWSLVSFKWNIKMPTIMSGSISILSDAGLGMAMFSLGLFMALQPKIIACGKSVAGFAMAVRFLTGPAVIAATSIAIGIRGDLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTAVIFGMLVALPVTVLYYVLLGL D0FAFF72031FFB33 pid_arath Protein kinase PINOID Protein kinase ABRUPTUS T31E10.1 PID ABR At2g34650 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLRESDGEMSLGTTNSPISSGTESCSSFSRLSFDAPPSTIPEEESFLSLKPHRSSDFAYAEIRRRKKQGLTFRDFRLMRRIGAGDIGTVYLCRLAGDEEESRSSYFAMKVVDKEALALKKKMHRAEMEKTILKMLDHPFLPTLYAEFEASHFSCIVMEYCSGGDLHSLRHRQPHRRFSLSSARFYAAEVLVALEYLHMLGIIYRDLKPENILVRSDGHIMLSDFDLSLCSDSIAAVESSSSSPENQQLRSPRRFTRLARLFQRVLRSKKVQTLEPTRLFVAEPVTARSGSFVGTHEYVAPEVASGGSHGNAVDWWAFGVFLYEMIYGKTPFVAPTNDVILRNIVKRQLSFPTDSPATMFELHARNLISGLLNKDPTKRLGSRRGAAEVKVHPFFKGLNFALIRTLTPPEIPSSVVKKPMKSATFSGRSSNKPAAFDYF DIP protein O64682 original sequence version: 112 DIP protein O64682 original sequence version: 79 29A33E80FF96B032 pini_arath Auxin efflux carrier component 1 Protein PIN-FORMED PIN1 F6D5.2 At1g73590 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MITAADFYHVMTAMVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIAANNPYAMNLRFLAADSLQKVIVLSLLFLWCKLSRNGSLDWTITLFSLSTLPNTLVMGIPLLKGMYGNFSGDLMVQIVVLQCIIWYTLMLFLFEYRGAKLLISEQFPDTAGSIVSIHVDSDIMSLDGRQPLETEAEIKEDGKLHVTVRRSNASRSDIYSRRSQGLSATPRPSNLTNAEIYSLQSSRNPTPRGSSFNHTDFYSMMASGGGRNSNFGPGEAVFGSKGPTPRPSNYEEDGGPAKPTAAGTAAGAGRFHYQSGGSGGGGGAHYPAPNPGMFSPNTGGGGGTAAKGNAPVVGGKRQDGNGRDLHMFVWSSSASPVSDVFGGGGGNHHADYSTATNDHQKDVKISVPQGNSNDNQYVEREEFSFGNKDDDSKVLATDGGNNISNKTTQAKVMPPTSVMTRLILIMVWRKLIRNPNSYSSLFGITWSLISFKWNIEMPALIAKSISILSDAGLGMAMFSLGLFMALNPRIIACGNRRAAFAAAMRFVVGPAVMLVASYAVGLRGVLLHVAIIQAALPQGIVPFVFAKEYNVHPDILSTAVIFGMLIALPITLLYYILLGL DIP protein Q9C6B8 original sequence version: 72 333E90DB12F62C83 pp2aa1-pin1 1 3 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component colocalization colocalization 5F Quantitative analysis demonstrated that about 7% of the detected PP2AA1 signal colocalizes with PIN1,whereas 80% of PIN1 overlaps with PP2AA1. The authors state that "Quantitative analysis demonstrated that about 7% of the detected PP2AA1 signal colocalizes with PIN1, whereas 80% of PIN1 overlaps with PP2AA1". pp2aa1-pin2 1 3 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role neutral component neutral component colocalization colocalization 5C Closer examination by immunolocalization revealed colocalization of a fraction of PP2AA1:GFP with PIN1 and PIN2 at the plasma membrane pid-pin2-1 1 5 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role neutral component neutral component colocalization colocalization 5I Quantitative analysis of colocalization revealed that 23% of PID:YFP colocalizes with PIN2, whereas 89% of PIN2 colocalizes with PID:YFP. The authors note that colocalisation occured in the apical plasma membrane of epidermal cells and that a "quantitative analysis of colocalization revealed that 23% of PID:YFP colocalizes with PIN2, whereas 89% of PIN2 colocalizes with PID:YFP". pid-pin1 2 6 enzyme target enzyme target substrate substrate neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 enzyme enzyme neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 7B GST:PID or HIS:PID autophosphorylates and efficiently phosphorylates the large hydrophilic loop of PIN1 (HIS:PIN1HL) and Myelin Basic Protein (MBP) (B) or the loop of PIN2 (HIS:PIN2HL) (C) in vitro. phosphorylation of PIN1 protein.