IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Xenopus Cep57 is a novel kinetochore component involved in microtubule attachment. Xenopus Cep57 is a novel kinetochore component involved in microtubule attachment. Cell (0092-8674) 2007 imex curation Emanuele MJ., Stukenberg PT. Xenopus eggs are cytostatic factor (CSF)-arrested. pts7h@virginia.edu 13-Nov-2007: Contacted by JYOTI. davet Only protein-protein interactions 7 xenla-oocyte Xenopus laevis oocyte Oocyte anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Emanuele MJ., Stukenberg PT. Xenopus eggs are cytostatic factor (CSF)-arrested. pts7h@virginia.edu Accepted 2007-NOV-18 by DAVET. 13-Nov-2007: Contacted by JYOTI. Cell (0092-8674) 2007 davet Only protein-protein interactions 7 imex curation Xenopus Cep57 is a novel kinetochore component involved in microtubule attachment. Xenopus Cep57 is a novel kinetochore component involved in microtubule attachment. Cell (0092-8674) 2007 imex curation Emanuele MJ., Stukenberg PT. Xenopus eggs are cytostatic factor (CSF)-arrested. pts7h@virginia.edu 13-Nov-2007: Contacted by JYOTI. davet Only protein-protein interactions 7 xenla-oocyte Xenopus laevis oocyte Oocyte fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Emanuele MJ., Stukenberg PT. Xenopus eggs are cytostatic factor (CSF)-arrested. pts7h@virginia.edu Accepted 2007-NOV-18 by DAVET. Cell (0092-8674) 2007 davet Only protein-protein interactions 7 imex curation zwint_xenla_protein ZW10 interactor protein protein xenla Xenopus laevis (African clawed frog) African clawed frog An amino acid sequence which is not represented in UniProt dctn1_xenla Dynactin subunit 1 dctn1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSVEATGKPLKVGSRVEVIGKGYRGTVAYVGATLFATGKWVGVILDDSKGKNDGTVQGRRYFTCEENHGIFVRQSQIQVIEDGADTTSPETPEPTASKGLKKDVMETPKSSKLPTRPSSSAASSGTASASCGEISSSEPSTPAQTPLAAPIIPSPSSAITSPVAPLPGPGPSKEEENLRAQVKDLEEKLETLKMKRAEDKAKLKEMEKSKLQLEQVQEWKSKMQEQQADIQRQLKEAKKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVDTLKDKVEEHKIDLEILKHEIEEKGSDGAASSYQVKQLEEQNARLKEALVRMRDLSASEKQEHIKVQKQMEKKNTELDTLRQQKEKLQEEASHMEKTIDELKEQVDAALGAEEMVETLAERNLDLEEKVRELRETVSDLEAINEMNDELQENARETELELREQLDMAGARVREAEKRVEAAQETVADYQQTIKKYRDLTAHLQEVNSELRNQQEASVEKEQQPSPEMFDFKIKFAETKAHAKAIEMELRKMEVTQANRHVSLLTSFMPDSFLRHGGDHDCILVLLLIPRLICKAELISKQAQEKFELSEVGEQKSGMRGAVGEQMSFAAGLVYSLTLLQATLHKYEQALDKCSVEVYKKVGMLYPEMSVHERSLDFLIELLHKDQLDETVNVEPLTKAIKYYQHLYSIHLADQAEECTMQLSDHIKFTQSALDCMGVEVSRLRAFLHAGQESSDFAILLKDLETSCSDIRQFCKKIRRRMPGTEAAGIPAALGFGQQVCETLLDCRKYLKCVVAVFQEVAAAGAQMIAPMGENEGLQALKLEDVAFKATEQIYGTKGSNPYECLRQSCSVVIATMNKMATAMQEGEYDAEKPQSKSPPPVEQRAAALRAEITDAEGLGLKLEDRETVIKELKKSLKIKGEELSEANVRLSLLEKKLDSASKEADDRVEKIQTKLEETQTVLKKKEKEFEETMDALQADIDQLESEKAELRQRLNNQSKRTIEGLRGVPASGVASIVSGLAGGVSSGQSLINGSGPVQVKDSPLLLQQIDALRLSMKHLKHENNKLKAHQIKTDLSSLPALHVPKLTLPKDRQKEEAMSGTLYRKTSQLLDALQQMSANAKVVDITHKKAGNPAAQLLEQTARLKSLSDTIDKLKNEVMKETVSQCPGANVPTDFATFPSTDFIKAKEEKKEDTVYIGKVTLSCQPGQGQIHKLVLTPEQLHELHERLIC ED5FC8DA07C93018 clip1_xenla_protein protein protein xenla Xenopus laevis (African clawed frog) African clawed frog An amino acid sequence which is not represented in UniProt a4ii13_xentr Protein MIS12 homolog LOC549562 protein protein xentr Xenopus tropicalis (Western clawed frog) Silurana tropicalis MSVHAMCYESQFFEFTPQTCILRVYIAFQDYLFEMLLVVEKVIMKKLESMEGSKISHFQIRESTEKYLNFIKERFNILFQKMESWLLKLVLSIPSNVLLLEDKVHAQYSYSKEEFEHLQTETETLQKKCKAESLAKQRLLAELEEQKQVQAELEKTLTWFDGLDKICREHGNIDLKESFGFMTQTSRKLQDKVKEIDMKHKKLKVDGSTLTPICPRIRKPK 9CB6DD19BC1F7591 cep57_xenla_protein Centrosomal protein of 57 kDa protein protein xenla Xenopus laevis (African clawed frog) African clawed frog An amino acid sequence which is not represented in UniProt ndc80_xenla Kinetochore protein NDC80 homolog kntc2 Kinetochore protein Hec1 Kinetochore-associated protein 2 ndc80 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MRRSSVTNRQSLLPLRVQDANRMGLTTPQNKDRQGFGKLSMSKPHSGTSEKKTSFFGKRASNGTARTSQYGAFGGTEKIKDPRPLHDKAFIQQCIRQLCEFLNENGYSQALTVKSLQGPSTKDFLKIFAFIYTFICPNYENPESKFEEEIPRIFKELGYPFALSKSSMYTVGAPHTWPQIVAALVWLIDCVKLCCVLRSENPVFEDPPMGEQSENGIDFNQLFLDYTVRCYDQFMEGRDTFEEYDTDVCIRLKEAYHVDESNLEALQQESRRLMEEIERLEKEKENEPDRLASMRKLKASLQADIQKYQNYLTEIESHSTLLDQRVSSISEELEATELESRAIQQENLRLKNILDNQKYSVADIERIKYEETELQQTIAKLTKDLDEDKQQLWHEELKYAKMKESVETQLSEFHKIGRKVRLIPPTAEFANGYDFQIQCNLDSEQSSLMHYRNKINVPLVEILSQSEGHIASSTNKKMGVEDMVEQLNTLIGEKKNEVKVQKDEAQKLEEIYQQKVEESEEHEKRWISEIESLEKHRQLLESGVNKSLDEAMKDLQKEQQELQLVEHRTEEEMRQVENKLVRVVHAVANHVAVIEKHLEEKRLKVEREYEEFMKEDLLLDLRELLEKYKEKARVLDSL 6D725844D425F29E tba_xenla Tubulin alpha chain tuba tuba1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVADITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGADSADAEDEGEEY 9A5B8497FA27FF8C kntc2-cep57-1 2 8 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization Confocal images of spindles immunostained with anti-xCep57, anti-Ndc80, and anti-tubulin antibodies Fig. 1E kntc2-cep57-2 1 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait association association Fig. 4B kntc2-cep57-4 1 8 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey association association Fig. 4B kntc2-cep57-5 1 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey association association Fig. 4B kntc2-cep57-6 1 8 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey association association Fig. 4B tuba-cep57-1 2 9 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization Immunofluorescent images localize xCep57 to the spindle poles and to punctuate double dots on metaphase chromatin (Merge: xCep57, red; tubulin, green; DNA, blue; scale bar = 5 μM). Fig. 1D kntc2-cep57-3 1 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey association association Fig. 4B