IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Purification of a plant mediator from Arabidopsis thaliana identifies PFT1 as the Med25 subunit. Purification of a plant mediator from Arabidopsis thaliana identifies PFT1 as the Med25 subunit. Mol. Cell (1097-2765) 2007 imex curation Backstrom S., Elfving N., Nilsson R., Wingsle G., Bjorklund S. The author has not responded to email requests to resolve enquires regarding what proteins coip with Med6. To reduce 'on hold' enteries the experiment and interactions are recorded from the paper as best I could do. Any suggestions welcome. A. thaliana cell suspension cultures (ecotype Columbia) were grown in MSAR media. Cells were maintained by weekly subculturing of 7.5 ml saturated culture into 42.5 ml of fresh MSAR and grown at under 24 degrees centigrade continuous light on a gyratory shaker at 120 rpm. All given TAIR identifiers were mapped to UniProt protein accession numbers using the PICR tool (https://webmail.ebi.ac.uk/picr). Some proteins could not be matched using PICR or by sequence similarity using MPsrch with a 98% query sequence similarity cutoff threshold. These proteins were : At2g48110, At1g44910, At1g23230, At4g04920, At1g15780, At5g03500 .The unmapped proteins were assigned protein identifiers of the type EBI-XXXXXXX and stored in the IntAct database for future identification. stefan.backstrom@medchem.umu.se 01-April-2008: Contacted by JYOTI. orchard Only protein-protein interactions arath-cell-culture Arabidopsis thaliana cell culture plant_cell_culture plant cellular culture The culture conditions should always be cited in the experimental modifications annotation Either whole plant or undifferentiated plant cells maintained in culture. anti bait coip anti bait coimmunoprecipitation anti bait coip fingerprinting peptide massfingerprinting fingerprinting Backstrom S., Elfving N., Nilsson R., Wingsle G., Bjorklund S. The author has not responded to email requests to resolve enquires regarding what proteins coip with Med6. To reduce 'on hold' enteries the experiment and interactions are recorded from the paper as best I could do. Any suggestions welcome. A. thaliana cell suspension cultures (ecotype Columbia) were grown in MSAR media. Cells were maintained by weekly subculturing of 7.5 ml saturated culture into 42.5 ml of fresh MSAR and grown at under 24 degrees centigrade continuous light on a gyratory shaker at 120 rpm. All given TAIR identifiers were mapped to UniProt protein accession numbers using the PICR tool (https://webmail.ebi.ac.uk/picr). Some proteins could not be matched using PICR or by sequence similarity using MPsrch with a 98% query sequence similarity cutoff threshold. These proteins were : At2g48110, At1g44910, At1g23230, At4g04920, At1g15780, At5g03500 .The unmapped proteins were assigned protein identifiers of the type EBI-XXXXXXX and stored in the IntAct database for future identification. stefan.backstrom@medchem.umu.se Accepted 2008-FEB-29 by ORCHARD. 01-April-2008: Contacted by JYOTI. Mol. Cell (1097-2765) 2007 orchard Only protein-protein interactions imex curation Purification of a plant mediator from Arabidopsis thaliana identifies PFT1 as the Med25 subunit. Purification of a plant mediator from Arabidopsis thaliana identifies PFT1 as the Med25 subunit. Mol. Cell (1097-2765) 2007 imex curation Backstrom S., Elfving N., Nilsson R., Wingsle G., Bjorklund S. The author has not responded to email requests to resolve enquires regarding what proteins coip with Med6. To reduce 'on hold' enteries the experiment and interactions are recorded from the paper as best I could do. Any suggestions welcome. A. thaliana cell suspension cultures (ecotype Columbia) were grown in MSAR media. Cells were maintained by weekly subculturing of 7.5 ml saturated culture into 42.5 ml of fresh MSAR and grown at under 24 degrees centigrade continuous light on a gyratory shaker at 120 rpm. All given TAIR identifiers were mapped to UniProt protein accession numbers using the PICR tool (https://webmail.ebi.ac.uk/picr). Some proteins could not be matched using PICR or by sequence similarity using MPsrch with a 98% query sequence similarity cutoff threshold. These proteins were : At2g48110, At1g44910, At1g23230, At4g04920, At1g15780, At5g03500 .The unmapped proteins were assigned protein identifiers of the type EBI-XXXXXXX and stored in the IntAct database for future identification. stefan.backstrom@medchem.umu.se 01-April-2008: Contacted by JYOTI. orchard Only protein-protein interactions arath-cell-culture Arabidopsis thaliana cell culture plant_cell_culture plant cellular culture The culture conditions should always be cited in the experimental modifications annotation Either whole plant or undifferentiated plant cells maintained in culture. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Backstrom S., Elfving N., Nilsson R., Wingsle G., Bjorklund S. The author has not responded to email requests to resolve enquires regarding what proteins coip with Med6. To reduce 'on hold' enteries the experiment and interactions are recorded from the paper as best I could do. Any suggestions welcome. A. thaliana cell suspension cultures (ecotype Columbia) were grown in MSAR media. Cells were maintained by weekly subculturing of 7.5 ml saturated culture into 42.5 ml of fresh MSAR and grown at under 24 degrees centigrade continuous light on a gyratory shaker at 120 rpm. Proteins were immunoprecipitation of a possible A. thaliana Mediator complex directly from the 200 mM potassium acetate eluate of the DEAE-Sephacel column. All given TAIR identifiers were mapped to UniProt protein accession numbers using the PICR tool (https://webmail.ebi.ac.uk/picr). Some proteins could not be matched using PICR. stefan.backstrom@medchem.umu.se Accepted 2008-FEB-29 by ORCHARD. 01-April-2008: Contacted by JYOTI. Mol. Cell (1097-2765) 2007 Only protein-protein interactions imex curation nrpa1_arath DNA-directed RNA polymerase I subunit 1 NRPA1 DNA-directed RNA polymerase I subunit RPA1 Nuclear RNA polymerase A1 RPA1 F15B8.150 At3g57660 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAHAQTTEVCLSFHRSLLFPMGASQVVESVRFSFMTEQDVRKHSFLKVTSPILHDNVGNPFPGGLYDLKLGPKDDKQACNSCGQLKLACPGHCGHIELVFPIYHPLLFNLLFNFLQRACFFCHHFMAKPEDVERAVSQLKLIIKGDIVSAKQLESNTPTKSKSSDESCESVVTTDSSEECEDSDVEDQRWTSLQFAEVTAVLKNFMRLSSKSCSRCKGINPKLEKPMFGWVRMRAMKDSDVGANVIRGLKLKKSTSSVENPDGFDDSGIDALSEVEDGDKETREKSTEVAAEFEEHNSKRDLLPSEVRNILKHLWQNEHEFCSFIGDLWQSGSEKIDYSMFFLESVLVPPTKFRPPTTGGDSVMEHPQTVGLNKVIESNNILGNACTNKLDQSKVIFRWRNLQESVNVLFDSKTATVQSQRDSSGICQLLEKKEGLFRQKMMGKRVNHACRSVISPDPYIAVNDIGIPPCFALKLTYPERVTPWNVEKLREAIINGPDIHPGATHYSDKSSTMKLPSTEKARRAIARKLLSSRGATTELGKTCDINFEGKTVHRHMRDGDIVLVNRQPTLHKPSLMAHKVRVLKGEKTLRLHYANCSTYNADFDGDEMNVHFPQDEISRAEAYNIVNANNQYARPSNGEPLRALIQDHIVSSVLLTKRDTFLDKDHFNQLLFSSGVTDMVLSTFSGRSGKKVMVSASDAELLTVTPAILKPVPLWTGKQVITAVLNQITKGHPPFTVEKATKLPVDFFKCRSREVKPNSGDLTKKKEIDESWKQNLNEDKLHIRKNEFVCGVIDKAQFADYGLVHTVHELYGSNAAGNLLSVFSRLFTVFLQTHGFTCGVDDLIILKDMDEERTKQLQECENVGERVLRKTFGIDVDVQIDPQDMRSRIERILYEDGESALASLDRSIVNYLNQCSSKGVMNDLLSDGLLKTPGRNCISLMTISGAKGSKVNFQQISSHLGQQDLEGKRVPRMVSGKTLPCFHPWDWSPRAGGFISDRFLSGLRPQEYYFHCMAGREGLVDTAVKTSRSGYLQRCLMKNLESLKVNYDCTVRDADGSIIQFQYGEDGVDVHRSSFIEKFKELTINQDMVLQKCSEDMLSGASSYISDLPISLKKGAEKFVEAMPMNERIASKFVRQEELLKLVKSKFFASLAQPGEPVGVLAAQSVGEPSTQMTLNTFHLAGRGEMNVTLGIPRLQEILMTAAANIKTPIMTCPLLKGKTKEDANDITDRLRKITVADIIKSMELSVVPYTVYENEVCSIHKLKINLYKPEHYPKHTDITEEDWEETMRAVFLRKLEDAIETHMKMLHRIRGIHNDVTGPIAGNETDNDDSVSGKQNEDDGDDDGEGTEVDDLGSDAQKQKKQETDEMDYEENSEDETNEPSSISGVEDPEMDSENEDTEVSKEDTPEPQEESMEPQKEVKGVKNVKEQSKKKRRKFVRAKSDRHIFVKGEGEKFEVHFKFATDDPHILLAQIAQQTAQKVYIQNSGKIERCTVANCGDPQVIYHGDNPKERREISNDEKKASPALHASGVDFPALWEFQDKLDVRYLYSNSIHDMLNIFGVEAARETIIREINHVFKSYGISVSIRHLNLIADYMTFSGGYRPMSRMGGIAESTSPFCRMTFETATKFIVQAATYGEKDTLETPSARICLGLPALSGTGCFDLMQRVEL 2F43F2907D4B2C8F med6_arath Mediator of RNA polymerase II transcription subunit 6 MED6_1 MHC9 At3g21350 MED6 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDSSLLSAATADTFNGNAADQIPPPLQPPGTDMTGISFRDQLWINSYPLDRNYIFDYFALSPFYDTTCNNEILRRRSIHPLDLSHLSKMTGLEYMLTDATEPNLFVFRKQKRDGPEKVTPMLTYYILDGSIYQAPQLCSVFAARVSRTIYNISKAFTDAASKLETIRQDLQVCLVAIVLSLSVNLGSYFLLIFKLMANGEQVYGFNKFLFDTENQNEPAESKPASETVDLKEMKRVDVILTSLYRKLAPPPPPPPFPEGYVSQEALGEKEELGTQGGESQPPQVDPIIDQGPAKRMKF DIP protein F4IXJ7 original sequence version: 22 000D39D2EDD7E4E7 md33a_arath Mediator of RNA polymerase II transcription subunit 33A MED33A MED24A MED5A MED5_2 REF4-related 1 protein REF4-resembling 1 protein RFR1 MDB19.7 At3g23590 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVVPGRRTVWDCVIELTKMAQENCVDPRLWASQLSSNLKFFAVELPSTELAEVIVSYICWDNNVPIVWKFLERAMALKLVSPLVVLALLADRVVPTRSTQQAAYRIYLELLKRNMFTIKDHISGPHYQKVMISVSNILRLSELFDLDTSKPGVLLVEFVFKMVSQLLDAALSDEGLLELSQDSSSQWLVKSQDMEIDAPERYNEKTGSLEKLQSLNTIMAIELIAEFLRNTVIARLLYLVSSNRASKWHEFVQKVQLLGENSSALKHSKVLNSGDLLQLISNRRFGYSYDSKVTSARKSNAIVDFGSLSSYAGLCHGASLSSLWLPLDLVFEDAMDGYQVNPTSAIEIITGLAKTLKEINGSTWHDTFLGLWIAALRLVQRERDPIEGPIPRLDTRLCMSLCIVPLVVANLIEEGKYESVMEKLRDDLVTSLQVLGDFPGLLAPPKCVVSAANKAATKAILFLSGGNVGKSCFDVINMKDMPVNCSGNMRHLIVEACIARNILDMSAYSWPGYVNGRINQIPQSLPNEVPCWSSFVKGAPLNAAMVNTLVSVPASSLAELEKLFEVAVKGSDDEKISAATVLCGASLTRGWNIQEHTVEYLTRLLSPPVPADYSRAENHLIGYACMLNVVIVGIGSVDSIQIFSLHGMVPQLACSLMPICEEFGSYTPSVSWTLPSGEAISAYSVFSNAFTLLLKLWRFNHPPIEHGVGDVPTVGSQLTPEHLLSVRNSYLVSSEILDRDRNRKRLSEVARAASCQPVFVDSFPKLKVWYRQHQRCIAATLSGLTHGSPVHQTVEALLNMTFGKVRGSQTLNPVNSGTSSSSGAASEDSNIRPEFPAWDILKAVPYVVDAALTACTHGRLSPRQLATGLKDLADFLPASLATIVSYFSAEVSRGVWKPVFMNGVDWPSPATNLSTVEEYITKILATTGVDIPSLAPGGSSPATLPLPLAAFVSLTITYKIDKASERFLNLAGPALECLAAGCPWPCMPIVASLWTQKAKRWFDFLVFSASRTVFLHNQDAVIQLLRNCFSATLGLNAAPMSNDGGVGALLGHGFGSHFYGGISPVAPGILYLRMYRALRDTVSVSEEILSLLIHSVEDIAQNRLSKEKLEKLKTVKNGSRYGQSSLATAMTQVKLAASLSASLVWLTGGLGVVHVLIKETIPSWFLSTDKSDREQGPSDLVAELRGHALAYFVVLCGALTWGVDSRSSASKRRRQAILGSHLEFIASALDGKISVGCETATWRTYISGLVSLMVSCLPLWVTEIDTEVLKSLSNGLRKWGKDELAIVLLSLGGLKTMDYAADFIIHLRS 4E85EBC186409D89 sdn3_arath Small RNA degrading nuclease 3 At5g67240 SDN3 K21H1.20 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEHKLATAEKKLLVDLVKLVQKRGLEGENGGWKEFLNVYDKKLGSSLSDPARRSNDVLVAFLLTFKKKEDLQLIARVMQCGANRELIEKFKQETPDKETPEQRLVRLTITHDDYPGNYTFPSYAEDWYVTELGKKKSKVIKSTRMLSIDCEMVTCEDGSQALVRVGAVDRDLKVVLDKFVKPDKPVIDYKTDITGVTAEDLERATLSVADIQKKLRRFLSVGTILVGHGLHNDLQVLRIDHARVIDTSYVFEFVDAPKTQRPSLNNLCKSVLGQEVRMDGAAHNCVHDAAAAMKLVLAAVEKGAATLIQPTEEMMVAEKRRQEARQEAGKAQLFLHKIPHDVPSEELHGVLSGNFTLVVKPPKTGGYSTAVVDFSSPEEANEAFENVEGDVAKDKSGLPQKKAVLKLSSGLAVSLFVRKMVQDDSPCEISTSERARAEENNVSSKRQKTEDETEETKEATVNQREADKTKLFLHKIPHDVPSQELHGVLNGDFTLDVKPPKRKGGYYNAVVDFNSPEEANEAFENVEGDVVKDKTGLPQKMVVFKLSSGSGVSLYVRKMVHDDSPGEISTTKRARTEESNMSSKRQKTEDESEETKEANAKQREADKTKLLLHKIPLNVPSQELKVVITGQFTLEVMPPKRKGRYYNAVVTFNSPEEANKAFEKVKGEAVKEKGGLAQKMVAFKLSSGSGACLYVRKMVQDESEETKEANANHCEDDHLKEMEELKEKLKAMEFAISCEGHSKEIEELKQKLNAKEHQIQAQDKIIANLKMKLEKKQSKSRS 427EDB62E043DBC5 med18_arath Mediator of RNA polymerase II transcription subunit 18 MED18 MED18_1 F14M13.23 At2g22370 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSMECVVQGIIETQHVEALEILLQGLCGVQRERLRVHELCLRSGPNLGVVSSEVRLLCDLDQPEPTWTVKHVGGAMRGAGADQISVLVRNMIESKVSKNALRMFYALGYKLDHELLKVGFAFHFQRTAHISVSVSSVNKMPKVHAIDEAVPVTPGMQIVDVTAPATSENYSEVAAAVSSFCEFLAPLVHLSKPSISTGVVPTAAAAAASLMSDGGGTTL FE53D1C081F36F55 med7a_arath Mediator of RNA polymerase II transcription subunit 7a MED7A MED7_1 F15A17.250 F9G14.160 At5g03220 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MATATYPPPPPYYRLYKDYSENPNSAPEPPPPIEGTYVCFGGNYTTEDVLPSLEEQGVPQLYPKDSNLDYKNELRSLNRELQLHILELADVLVDRPSQYAKRIGEISSIFKNLHHLLNSLRPHQARATLIHIMELQIQQRKQAVEDIKRRREEAQRLLKDAYLTLDGQ 65F18BBE9588207A md22b_arath Mediator of RNA polymerase II transcription subunit 22b MED22_1 T6D22.4 At1g07950 MED22B protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MNKGGGSGGGSGGGSGPTAAAAAAALQKQKALLQRVETDITSVVDNFTQIVNVSRVSDPPVKNSQETYMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIEEFDQEAEKTNRLLARIADDASANLKELESHYYSSAQRLTLDI B456EE8594840519 med25_arath Mediator of RNA polymerase II transcription subunit 25 MED25 MED25_1 PFT1 Phytochrome and flowering time 1 protein Phytochrome and flowering time regulatory protein 1 F2J7.4 At1g25540 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSSEVKQLIVVAEGTAALGPYWQTIVSDYLEKIIRSFCGSELNGERNPVSTVELSLVIFNSHGSYCACLVQRSGWTRDVDIFLHWLSSIQFGGGGFNEVATAEGLAEALMMFSPPSGQAQPSNDLKRHCILITASNPHILPTPVYRPRLQNVERNENGDAQAESRLSDAETVASYFAKCSVSLSVVCPKQLPTIRALYNAGKPNQQSADLSIDTAKNTFYLVLISENFVEACAALSHSATNLPQTQSPVKVDRATVAPSIPVTGQPPAPVSSANGPIQNRQPVSVGPVPTATVKVEPSTVTSMAPVPSFPHIPAVARPATQAIPSIQTSSASPVSQDMVSNAENAPDIKPVVVSGMTPPLRTGPPGGANVNLLNNLSQVRQVMSSAALAGAASSVGQSAVAMHMSNMISTGMATSLPPSQTVFSTGQQGITSMAGSGALMGSAQTGQSPGPNNAFSPQTTSNVASNLGVSQPMQGMNQGSHSGAMMQGGISMNQNMMSGLGQGNVSSGTGGMMPTPGVGQQAQSGIQQLGGSNSSAPNMQLSQPSSGAMQTSQSKYVKVWEGNLSGQRQGQPVLITRLEGYRSASASDSLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMSQHGFLGQLQDKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPGDMVVFKPQIPNQQQQQQQQLHQQQQQQQQIQQQQQQQQHLQQQQMPQLQQQQQQHQQQQQQQHQLSQLQHHQQQQQQQQQQQQQHQLTQLQHHHQQQQQASPLNQMQQQTSPLNQMQQQTSPLNQMQQQQQPQQMVMGGQAFAQAPGRSQQGGGGGQPNMPGAGFMG 9A9C81527BE4F384 med9_arath Mediator of RNA polymerase II transcription subunit 9 MED9_1 T7N22.3 At1g55080 MED9 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDQFSGGGGNWSMIPNVQAQGNFGTPTNHDQQLFLQQQQLQQQQQQQQQQQFHLQQQQQTQQQQQQFQPQQQQEMQQYQQFQQQQHFIQQQQFQQQQRLLQSPPLQPQSLQSPPPQQTMVHTPQSMMHTPQQQQQLVQTPVQTPQQHQSLASHFHLYPMVEKLADVIENGTRDQNSDALVNELNSHFDKCQQLLNSISGSLGSKTMTVDGQKRNVEESEQLLQQRRDLIVEYRKSIEEIVTMEH BF795E4ED4047FEE nrpbc_arath DNA-directed RNA polymerases II, IV and V subunit 12 NRPB12 DNA-directed RNA Polymerase II subunit K NRPD12 NRPE12 MEE6.8 At5g41010 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDPAPEPVTYVCGDCGQENTLKSGDVIQCRECGYRILYKKRTRRVVQYEAR B3B32CFFC943AE5A nrpa2_arath DNA-directed RNA polymerase I subunit 2 DNA-directed RNA polymerase I subunit RPA2 Nuclear RNA polymerase A2 NRPA2 RPA2 F1N18.2 T1P2.15 At1g29940 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVVNAKDSTVPTMEDFKELHNLVTHHIESFDYMTLKGLDVMFNRIKPVSVYDPNTENELSIWLENPLVFAPQKESFKSTSRKEPLLPFECRQAKISYTGTFMADVCFKYNDGVVVRDKFDFGQFPIMLMSKLCSLKGADCRKLLKCKESTSEMGGYFILNGIERVFRCVIAPKRNHPTSMIRNSFRDRKEGYSSKAVVTRCVRDDQSSVTVKLYYLRNGSARVGFWIVGREYLLPVGLVLKALTNSCDEEIYESLNCCYSEHYGRGDGAIGTQLVRERAKIILDEVRDLGLFTREQCRKHLGQHFQPVLDGVKKESLSIVAEAVLRDYLFVHLDNDHDKFNLLIFIIQKLYSLVDQTSLPDNPDSLQNQEILVPGHVITIYLKEKLEEWLRKCKSLLKDELDNTNSKFSFESLADVKKLINKNPPRSIGTSIETLLKTGALKTQSGLDLQQRAGYTVQAERLNFLRFLSFFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGTPCGLLNHMTRTSRITSQFDSKGNIRDFLKIRKSVVDVLTGAGMVPSLPKLVRAGPPKVIHVLLDGQVVGTLSSNLVTKVVSYIRRLKVEAPSVIPEDLEVGYVPTSMGGSYPGLYLASCPARFIRPVKNISIPSDNIELIGPFEQVFMEISCPDGGNGGRNNSSLATHEEIHPTGMISVVANLTPWSDHNQSPRNMYQCQMAKQTMAYSTQALQFRADQKIYHLQTPQSPVVRTKTYTTYSIDENPTGTNAIVAVLAHTGFDMEDAMILNKSSVERGMCHGQIYQTENIDLSDQNSRFDSGSKSFRRSTNKAEHFRIDADGLPSVGQKLYPDEPYCSIYDEVTNKTRHMKRKGTDPVIVDFVSVDMKSKKHPQRANIRFRHARNPIIGDKFSSRHGQKGVCSQLWPDIDMPFNGVTGMRPDLIINPHAFPSRMTIAMLLESIAAKGGSLHGKFVDATPFRDAVKKTNGEEESKSSLLVDDLGSMLKEKGFNHYGTETLYSGYLGVELKCEIFMGPVYYQRLRHMVSDKFQVRSTGQVDQLTHQPIKGRKRGGGIRFGEMERDSLLAHGASYLLHDRLHTSSDHHIADVCSLCGSLLTSSVVNVQQKKLIQEIGKLPPGRTPKKVTCYSCKTSKGMETVAMPYVFRYLAAELASMNIKMTLQLSDREGVTD 9660FCAEF8FD6086 tgt3b_arath Trihelix transcription factor GT-3b GT1L GT3B Transcription factor GT-1-like Trihelix DNA-binding protein GT-3b F16M14.18 GT-3B At2g38250 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDGHQHHHLHQLQYLNKHHLHTQSQTPEIASPVAVGDRFPQWSVEETKELIGIRGELDQTFMETKRNKLLWEVISNKMRDKSFPRSPEQCKCKWKNLVTRFKGCETMEAETARQQFPFYDDMQNIFTTRMQRMLWAESEGGGGGTSGAARKREYSSDEEEENVNEELVDVSNDPKILNPKKNIAKKRKGGSNSSNSNNGVREVLEEFMRHQVRMESEWREGWEAREKERAEKEEEWRRKMEELEKERLAMERMWRDREEQRRSREEMRAEKRDSLINALLAKLTRDGSL CEE46E6022724754 med11_arath Mediator of RNA polymerase II transcription subunit 11 MED11 MED11_1 T13O15 At3g01435 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDPQTQNTSLQRLQNVENRVVKVLELAGGVMEELASPSGPKKEFVNSHCREFMQSMKDIQVTLREEIKSACEYRPFEKCDYNARIANEICFQKLEYVLTQLEDLKQTADRYPSSD 0A667258161F58C6 med30_arath Mediator of RNA polymerase II transcription subunit 30 MED30 MED30_1 MLE2.11 At5g63480 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLQKQVSTTTTMTTQELAMEGEKQLEETIEAAFQIISAMNDELCNPSLWSTSATPSSAATTTGSNGSALVSADAAAIDGTSHHSESAGGGGGGGSGNSVLDEASLRYKNSVTSLRAVLAAIPNSQKAKASEMQNGLGSPESEDEIEKLEEQALSLRMEIAKKNVHVKELIDKLRELIADISTWQSPCSV 23BCD525B18004C2 stkls_arath GLABROUS1 enhancer-binding protein-like GEBPL Mediator-associated protein 1 Protein GeBPL Storekeeper-like protein At4g25210 Transcription factor At4g25210 F24A6.50 At4g25210 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAPKKAEEVVESPPVSSEEEESGSSGEESESSAEVPKKVESSQKPESDSEGESESESSSGPEPESEPAKTIKLKPVGTKPIPETSGSAATVPESSTAKRPLKEAAPEAIKKQKTSDTEHVKKPITNDEVKKISSEDAKKMFQRLFSETDEIALLQGIIDFTSTKGDPYEDIDAFCIYVKKLIDFDATKNQIVTKLQRLKKKFNNAVKNSLKKGKTEDDIEFAKDLEQKGFELSRKIWGSNGVLVTGKSSRKKVGGTPAPKEMKLVAHSTPKKQQEEAKKPERTEAKVVNTGLSIGKEIASFLNADNGSSCGLDESTLTAVWAKVADGAEKREVEEKWKKLKAKQFELCLQRSGLVNETAKMIFKAYES 52B8EF99109BC8E4 rpb8b_arath DNA-directed RNA polymerases II, IV and V subunit 8B NRPB8B NRPD8B NRPE8B RNA polymerase Rpb8 T16L24.150 At3g59600 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASNIIMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPTLNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAELYVSFGGLLMLLQGDPAHISHFELDQRLFLLMRKL 230F6E9995E368B7 med31_arath Mediator of RNA polymerase II transcription subunit 31 MED31 MED31_1 Mediator complex subunit 31 Mediator complex subunit SOH1 F28I16.60 At5g19910 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASPEEMGDDASEIPSPPKNTYKDPDGGRQRFLLELEFIQCLANPTYIHYLAQNRYFEDEAFIGYLKYLQYWQRPEYIKFIMYPHCLYFLELLQNPNFRTAMAHPANKELAHRQQFYYWKNYRNNRLKHILPRPLPEPVPPQPPVAPSTSLPPAPSATAALSPALSPMQYNNMLSKNDTRNMGATGIDRRKRKKGI 706E3EB75C8F0A19 med32_arath Mediator of RNA polymerase II transcription subunit 32 MED32 MED2 MED29 MED2_1 Mediator of RNA polymerase II transcription subunit 2 F25C20.9 At1g11760 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDNIVDSLNKAYEKFVLASAGVLESKESAGGQKALLTDTALENFKEKWELFRVACDQAEEFVESVKQRIGSECLVDEATGLTTTAAGGQAPAAVTGAATSLPPISAVRLEQMSRAVRWLVLELQRGSGVAPGSVHSSSTGFDSRFSEDSTQ 9D2B07AECF1C9F30 rpb8a_arath DNA-directed RNA polymerases II and V subunit 8A NRPB8A NRPE8A RNA polymerase I, II and III 16.5 kDa subunit RPABC16.5 F20D21.7 At1g54250 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASNIILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPTLNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAELYVSFGGLLMLLKGDPAHISHFELDQRLFLLMRKL F4EB3FB9C4DE4D75 med8_arath Mediator of RNA polymerase II transcription subunit 8 MED8_1 Protein SETH 10 SETH10 T17M13.24 At2g03070 MED8 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress METQPQQPPPPPVAEKLNPKLEKELNLESLKTRAVSLAKAIARILEDFDAYGRTNTTPKWQDILGQYSMVNLELFNIVEEVKRVSNAFVVLPKNVNAMNAAILPVMLSSKLLPEMETDDNAKREQLLQGVQSLPIPMQIERLKARMDMIAAACENAERVLADTRKAYGFGTRQGPSMLPTMDKGQAAKIQEQEKMLRDAVNDGKGTQLPPDQRQITTALPPHLADVLIINDAGKIALPGQSNNINNQGMMQVSGTQFVGRSAASPSGPNFDNTTSPLPYSNSPRATGMVNVPSPQHQIQQQQFQQQQQRSKLMQLPQHQQQQLLAQQQQQLRQSSMQGLGQSQIPALHDMHGQAQQKFQTSHGQHQMPYSQPMGAHQQFQARQLSGGHIQHSMSQGQLNPAMNRHLNQFSGGANSALFTSAQGSPSSQMIPNMSSMQSQTLVPRMQQFGVSGTNPQRSHSSQMLGDQMFNTSGMMQTQQTQIQQSQQQQQQQQQGGYGNMQTNQQSLQPNNMMQNAQQRHQNPQ 8DB534DFA531189A med27_arath Mediator of RNA polymerase II transcription subunit 27 MED27 MED3 MED3_1 Mediator of RNA polymerase II transcription subunit 3 F3L24.5 MZB10.22 At3g09180 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQTLHQSQLLQNPAEAANNQSESDAPPKQVAQAMERLNQAARVIADIRLGADRILEAMFVASQPRHTDMPLQLFLREDASMRQHLQDLRLIGKKLEESGVLTESLRSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHIDDGLKMEPSSKKHRASHLLLENGREEPVDYKTLPDIQSRLEKLVPSVKVSTYGRLNWLKRANSLPGSGSDDPTEASKPIFQSSSKLRSGLQTEVVDKIAVIELSFPSLFRAIVSLSPAGSVDPDAVAFFSPDEGGSYLHARGFSVYHVYKHITEHAATALQYFLGFGTGTALYSLLLWICSFESVFSKPCTKCGRLLAMDKKSALILPPLHRAYQELPLALNLDVCEAYHSSCSQDDT F780151B45FF3A11 q42483_arath AtRPAC14 ATRPAC14 ATRPC14 RNA POLYMERASE 14 KDA SUBUNIT RNApolymerase 14 kDa subunit RPC14 F16P2.8 F16P2_8 At2g29540 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEHGSFTNVSHASFTLSEEDHTLANAVRFVLNQDPRVTVAAYTIPHPSLEQVNIRVQTTGDPAREVFKDACQELMQMNRHVRSVFDKAVAEYKDEQKRKEEAEEEELKRQRDLFGSMDIENN 19F9106400768094 med17_arath Mediator of RNA polymerase II transcription subunit 17 MED17 MED17_1 F5O24.60 At5g20170 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDSDMEISLDRLPIKRLESIEENGAERFPSDVDYDDKRVSLIRRIDFAWALEEEDELKKKKQKKSSKDSVEQWKWKGMVENLQLAHQELTVIIDLIDTVQANDAVTVAGMTRPKPMPNEILSDLAVSTATKLQGYRNLGNYFKQSAKALEQKINREARFYGALIRLQRNWKVKRQRMLASNASNEGFTIDLSDSSLYDPTSGFRPSTLSTIRVDHDSAGMLAINVPQDSWYSLRFGYVGLNPIGNSNESDEHIDSTTGHDIPGTSEKLSASDDKYVKETHSLLREVHKSIFAEQLFDMLNREAFNEGVGFNISGLRENFMEMSIGQGASLFVSLHPSGKNPSIKKSESATLLIESSGRVEPAEGGDYRLKKLGFPNRTSYEIYLQQIFHEHAFGKAKDQLKSKSIRASNQTEKDSNSGLLDHFCLSLTHRIFSNRVLVHLESVVCKVPYLHLISHPTWNSRTSSWTVFMTVPPSIIPQGRSETQSPDGKRNLKTQFRTKVVVKDDCISVEAECTPNVVGLLKSSSCNLFSINKYECDVADLPVMILQQVASQIVCWLLEEARTVGTKASREFLSLSLEIVEGERVSLVAHVNPEDAKGCISWWLVMENGCTEEREGVSESRKLLGHLSLDVLYSVLMDLINLCGTGRNALERL D06CDC043C9AA5AD med4_arath Mediator of RNA polymerase II transcription subunit 4 MED4 MED4_1 F9G14.160 At5g02850 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLQHQIVQSPARLGLTGPGSPSVQNPTPTRHGHPTSSSSSQSQHQQIQQQPNLLPSSTVAAASSASASAAVSSSALLSLLPPLPRAQALLQQMAVLTSKLFDVSPNRAIWLSAFRGSLPSFLSSHSLPPPPPLENPSPSSTKEILSQFNSLQTQLFEAVTELQEILDLQDAKQKVAREIKSKDSSLLAFANKLKDAERVLDMLVDDYSDYRKPKRSKIEEDDEDNDNESSSSSTTVSSQLKLKDILAYAHKISYTTFAPPEFGAGQAPLRGALPPAPQDEQMRASQLYTFADLDIGLPKTVENMEKKVEALIEPPPPPEAMDISAIHNLLPPNIAVPSGWKPGMPVELPRDLPLPPPGWKPGDPVVLPPLESIAAPRAEDHQHMRPSQGLHRPPDVIQVRAVQLDILEDDDSSDYSSDDASSDDED 4AC6A38586479FCE f4hrl9_arath ATRPAC42 AAC42 RNA POLYMERASE I SUBUNIT 42 F23C21.3 F23C21_3 At1g60850 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVTKAEKQFAKNFNIDDLPDVPAGLPPHLKAQQTRVVSKNNAPAHTASAIYSGTYVSSTEEDDNVKLGNFYDNFKVDVVSLTKTDMEFDMIGIDAAFANAFRRILIAEVPSMAIEKVLIAYNTSVIIDEVLAHRMGLIPIAADPRLFEYLSEHDQANEKNTIVFKLHVKCPKNRPRLKVLTSDLKWLPNGSELLRESENKTSKPKTYTSFSCSQDSLPEFANNPITPCDLDILIAKLAPGQEIELEAHAVKGIGKTHAKWSPVGTAWYRMHPEVRSIYINLSYALWETALCFVFPHPNIYVS 340ABD7F0E10CC3B med28_arath Mediator of RNA polymerase II transcription subunit 28 MED28 MED28_1 F8J2.30 At3g52860 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDYQQKPPQSSDPSPSPPDRPPGIRSPETPSNNQNNDIEDIMACVTALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFMGLKREDRAPSRAESLKKDIAVMEEELKTKDELIKKHMRLFQESQKLVKEQIEKHRDELEKV 43C35226F4041346 mda2_arath Mediator-associated protein 2 MUB3.20 At5g64680 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDFDFKVSGDFIVSGAEQLDDTDLTRSDEFWLIQAPLGQFPEIEENTLKIEPDKDGLFGEFKDSNAGSLHLCLGAKYDLASFHSQDAGAELIIPSEESMIVGKITRRVALVRYPEPNELLQKMKARTQQKLVGSVTNSSKKSSNLTQSSRHKSGTRSSREKSMFSGFTETPKSPKRKNSESSSGKHRSSTSTVSGSSERSAKSKKKVKKEE FFCAB7DDB9A4B760 med14_arath Mediator of RNA polymerase II transcription subunit 14 MED14 MED14_1 Protein STRUWWELPETER SWP F7O18.23 At3g04740 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAELGQQTVDFSALVGRAAEESFLSFKELVDKSKSTELSDTEKKVSLLKYVAKTQQRMLRLNALAKWCKQVPLINYFQDLGSTLSAHDICFTQAADSLFFMHEGLQQARAPVYDVPSAVEILLTGSYQRLPKCLDDVGMQSSLDEHQQKPALRKLEVLVRSKLLEITLPKEITEVKISKGTVTLSVDGEFKVLVTLGYRGHLSMWRILHLDLLVGERSGPIKLEVTRRHILGDDLERRMSVAENPFTILYAVLHELCVAIVMDTVIRQVRALLQGRWKDAIRFDLISDTGTTPANQEGEADSVSLRTPGMKLFYWSDSDKNSGPFIKIEPGSDLQIKCSHSTFVIDPLTGKEAEFSLDQSCIDVEKLLLKAICCNRYTRLLEIQKELLRNTRICRTPSDVILQALLDEPGIEGDNMVDSKERVEPEVLRVRAYGSSFFTLGINIRTGRFLLQSSKSILTSSILEEFEDALNQGSISAVDAFINLRSKSILHFFAAIGKFLGLEVYEHGFGINKVPKSLLDGSSILTLGFPDCESSHLLLMELEKDFTPLFKLLETQMDGSGKPQSFNDPSNILRAKKIDIGQIRILEDDLNLITSDVVKFVSSFSDAEGINQASGHRQPGLVDEALTEMSGSQLSFSSVVDGVFGLQKVTSALMSIDGHGLVPKNLSAVTGHGKAPMLTSYHSDSLYNRQGPLQSSSYNMLSSPPGKGSAMKKIAISNSDQELSLILSPSLSTGNGVSESGSRLVTESSLSPLPLSQTADLATSSAGPLLRKDQKPRKRSASDLLRLIPSLQVVEGVASPNKRRKTSELVQSELVKSWSPASQTLSTAVSTSTKTIGCSYGNLIAEANKGNAPSSVFVYALLHVVRHSSLSIKHAKLTSQMEALDIQYVEEMGLRDAFSDIWFRLPFAQNDSWQHICLQLGRPGSMCWDVKINDQHFRDLWELQKGSKTTPWGSGVHIANSSDVDSHIRYDPEGVVLSYQSVEADSIKKLVADIQRLSNARMFSLGMRKLLGIKPDEKTEECSANSTMKGSTGGKGSGEPVDRWRAFKIEAVGLTSLWFSFGSGVLARFVVEWESGKDGCTMHVSPDQLWPHTKFLEDFINGAEVESLLDCIRLTAGPLHALAAATRPARASTATGMPVVPATASSRQSNQIQQTQGIIAPSTLAAPNATGQSASATSGNTVASSAPSPLGGGFHGVAMLAAAGRSGPGIVPSSLLPIDVSVVLRGPYWIRIIYRKRFAVDMRCFAGDQVWLQPATPPKGGASIGGSLPCPQFRPFIMEHVAQELNGLEPNLTGSQGATNPNSGNPTVNGVNRVNFSPSSARAAMNRVASVASGSLVVSSGLPVRRTPGTAVPAHVRGELNTAIIGLGDDGGYGGGWVPLVALKKVLRGILKYLGVLWLFAQLPDLLREILGSILKDNEGALLNLDQEQPALRFFVGGYVFAVSVHRVQLLLQVLSVRRFHHQAQQNGSSAAAQEELTQSEIGEICDYFSRRVASEPYDASRVASFITLLTLPISVLREFLKLIAWKKGLSQSQQAGEIAPAQRPRIELCLENHSGTDLDNNCAAKSNIHYDRPHNTVDFALTVVLDPVHIPHINAAGGAAWLPYCVSVRLRYTFGENPSVTFLGMEGSHGGRACWQRVDDWEKCKQRVSRTVEVNGSAAGDLTQGKLKLVADSVQRTLHLCLQGLREGGNNNNNTHQKEFTI 5C68D7E02FB66A39 md10b_arath Mediator of RNA polymerase II transcription subunit 10b MED10B MED10_1 T24P13.4 At1g26665 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDPTQNTSAGIGGSNGTIRYQTNDGTSTVTVADDSKENLSQVINSIEKTLGVLHQLHLTVTSFTPASQLHLLQRLNSLVMELDNMTKLSEKCNIQIPMEVLNLIDDGKNPDEFTKDVLNSCIARNQVTKGKTDAFKDLRKHILEELEQNFPDEVDMYREIRASSAAVTKRLAQSQSVLPNGDAKVKNEL C654B120A63A0FAC fbrl2_arath rRNA 2'-O-methyltransferase fibrillarin 2 FIB2 FLP Fibrillarin-like protein 2 Histone-glutamine methyltransferase MED36A MED36_1 L73G19.10 At4g25630 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MRPPLTGSGGGFSGGRGRGGYSGGRGDGGFSGGRGGGGRGGGRGFSDRGGRGRGRGPPRGGARGGRGPAGRGGMKGGSKVIVEPHRHAGVFIAKGKEDALVTKNLVPGEAVYNEKRISVQNEDGTKTEYRVWNPFRSKLAAAILGGVDNIWIKPGAKVLYLGAASGTTVSHVSDLVGPEGCVYAVEFSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKSGGHFVISIKANCIDSTVPAEAVFQTEVKKLQQEQFKPAEQVTLEPFERDHACVVGGYRMPKKPKAATAA DIP protein Q94AH9 original sequence version: 65 0E3DF1733F52CFDC gyra_arath DNA gyrase subunit A, chloroplastic/mitochondrial GYRA At3g10690 F13M14.2 T7M13.23 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTPVLCHSTASIPNPNSLMSLSSTLRLSSSLLRRSFFRFPLTDPLCRLRRTEPSATRFFSSRTPRSGKFVVGAGKRGDEQVKEESGANNGGLVVSGDESRIVPFELHKEATESYMSYALSVLLGRALPDVRDGLKPVHRRILFAMHELGMSSKKPYKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQSFSLRCPLIQGHGNFGSIDADPPAAMRYTECRLDPLAEAVLLSDLDQDTVDFVANFDNSQKEPAVLPARLPALLLNGASGIAVGMATNIPPHNLGELVDVLCALIHNPEATLQELLEYMPAPDFPTGGIIMGNLGVLDAYRTGRGRVVVRGKAEVELLDPKTKRNAVIITEIPYQTNKATLVQKIAELVENKTLEGISDIRDESDRNGMRVVIELKRGGDPALVLNNLYRHTALQSSFSCNMVGICDGEPKLMGLKELLQAFIDFRCSVVERRARFKLSHAQQRKHIIEGIVVGLDNVDEVIELITKASSHSSATAALQSEYGLSEKQAEAILEITLRRLTALERKKFTDESSSLTEQITKLEQLLSTRTNILKLIEQEAIELKDRFSSPRRSMLEDSDSGDLEDIDVIPNEEMLMAVSEKGYVKRMKADTFNLQHRGTIGKSVGKLRVDDAMSDFLVCHAHDHVLFFSDRGIVYSTRAYKIPECSRNAAGTPLVQILSMSEGERVTSIVPVSEFAEDRYLLMLTVNGCIKKVSLKLFSGIRSTGIIAIQLNSGDELKWVRCCSSDDLVAMASQNGMVALSTCDGVRTLSRNTKGVTAMRLKNEDKIASMDIIPASLRKDMEEKSEDASLVKQSTGPWLLFVCENGYGKRVPLSSFRRSRLNRVGLSGYKFAEDDRLAAVFVVGYSLAEDGESDEQVVLVSQSGTVNRIKVRDISIQSRRARGVILMRLDHAGKIQSASLISAADEEETEGTLSNEAVEAVSL 3998C233EA512797 md10a_arath Mediator of RNA polymerase II transcription subunit 10a MED10_2 MJC20.1 At5g41910 MED10A protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDQTQNTIAGIGEINGSIKTEINAIATVDDSNEKLNQIINSNQKILELLHQLKLTVSSFTPASQLHLLQRLNSLVMELDNMAKLSDKCNIQVPIEVLNLIDDGKNPDEFTKDVLNKNCIAKNQVTKGKSDAFKGLRKHLLEELEQAFPDEVDRYRDIRASYAAEAKRLAQTQSVLPNGDAKVKSEL F0D36D9DAC5DC160 md22a_arath Mediator of RNA polymerase II transcription subunit 22a MED22A MED22_2 F3O9.23 At1g16430 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MMNKGGGSGGGSGPTAAAAAAALQKQKALLQRVDTDITSVVDNFNQIVNVARVSDPPMKNSQEAYMMEMRASRLVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIAEFDQEAEKTNRLLARIADDASASLKELESHYYSSAQRSTLD 79315188A83381E2 md20a_arath Mediator of RNA polymerase II transcription subunit 20a MED20A MED20_1 T3B23.10 At2g28230 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MPVKWVLHWQPNQGSTVSSQILNEATQCVESINGVKEGRWKATLNYYKPMLKDQANQLEFPRDFLGISLADQPNKYYFIIRTQRIVLEADSSIQLIMEKLQSYKSKVALYFDGFQYQLGDFRLRVGKVVPTHSENVRGIVMEVEYLPISSMEKAQKVMEEFLEIWNEALAKRSLPGKFVNIDLNFGEFGLGDIYTPQHTAVRYALVMAHMIATVQAVRG C3AC5D1BBA3936EC mda3_arath Mediator-associated protein 3 DEK-domain containing protein At5g42060 KELP-like protein MJC20.17 At5g42060 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEVAREEVEIDKDLRRKIKKTVKKILESSNLYKITEIKAREEASLKLDLDLSQDPYKVIVKEEVENFLEEAVKLIGNKLAMLPKRIESTSI 217732172809BE69 recq2_arath ATP-dependent DNA helicase Q-like 2 RECQL2 MED34 MED34_1 RECQ2 RQL2 RecQ-like protein 2 T19E23.16 At1g31360 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MESEAIQEDLQNLDVELKDVQGQISALIEHQDRLYERKSELKTLLKALAASGSPVASSGGSSAIENWSETFEWDSRADDVRFNVFGISKYRANQKEIINAIMTGRDVLVIMAAGGGKSLCYQLPAMLRGGTTLVVSPLLSLIQDQVMGLAALGISAYMLTSTSGKENEKFVYKALEKGEDDLKILYVTPEKVSKSKRFMSKLEKCHNAGRLSLISIDEAHCCSQWGHDFRPDYKNLSILKTQFPKVPMVALTATATQKVQNDLIEMLHIPKCVKFVSSVNRPNLFYSVREKSAVGKLVVDEIAEFIRESYSNNESGIVYCFSRKECEQIAGDLRERGISADYYHADMDANMREKVHMRWSKNKLQVIVGTVAFGMGINKPDVRFVIHHSLSKSMETYYQESGRAGRDGLPSECILFFRSADVPRQSSMVFYEYSGLQNLYDIVRYCQSKTKCRRSAFFRHFGEPSQDCNGMCDNCALSSEVKEVDVSDLSKLVVSMVQETQAKDQRVTMLQLGDKLRNKHKDLIAELKRDEVEHLVIKLIVDSVLKEEFQHTPYSTNAYVTMGPLANQLLQGRKTIKMETSSRQTKKLKRSITFSGLELKLDELRKEISAADGSILPHTVLSTQQIGSISSQKPVSLQELESIIGKLKTEKYGDRILEEVMRHEAVSEQLVEDPTKEETCKSRLRKRAKTQKDVVLVESSGEEEA 7976A97A8015DD90 q9lu97_arath DNA-directed RNA polymerase subunit At3g25940 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEKSRESEFLFCNLCGTMLVLKSTKYAECPHCKTTRNAKDIIDKEIAYTVSAEDIRRELGISLFGEKTQAEAELPKIKKACEKCQHPELVYTTRQTRSADEGQTTYYTCPNCAHRFTEG 08A40715558D67A0 md19a_arath Mediator of RNA polymerase II transcription subunit 19a MED19_1 MXC9.19 At5g12230 MED19A protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEPERLKFGGPRELCGAADLISQFKLVQHHEFFCKKSLPVSLSDSHYLHNVVGDTEIRKGEGMQLDQLIESISQSRETNIRIQPFDIDELQESFQLNDMTPVELPPAEKGAPTIPSKSKSESKDRDRKHKKHKDRDKDKDREHKKHKHKHKDRSKDKDKDKDRDRKKDKNGHHDSGDHSKKHHDKKRKHDGDEDLNDVQRHKKNKHKSSKLDEVGAIRVAG DIP protein Q9FMP0 original sequence version: 56 177F2531AD9E599B bip1_arath Heat shock 70 kDa protein BIP1 BIP1 HSP70-11 Heat shock 70 kDa protein 11 Heat shock protein 70-11 Luminal-binding protein 1 MED37A MED37_5 T26D3.10 T26D3_50 At5g28540 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MARSFGANSTVVLAIIFFGCLFALSSAIEEATKLGSVIGIDLGTTYSCVGVYKNGHVEIIANDQGNRITPSWVGFTDSERLIGEAAKNQAAVNPERTVFDVKRLIGRKFEDKEVQKDRKLVPYQIVNKDGKPYIQVKIKDGETKVFSPEEISAMILTKMKETAEAYLGKKIKDAVVTVPAYFNDAQRQATKDAGVIAGLNVARIINEPTAAAIAYGLDKKGGEKNILVFDLGGGTFDVSVLTIDNGVFEVLSTNGDTHLGGEDFDHRVMEYFIKLIKKKHQKDISKDNKALGKLRRECERAKRALSSQHQVRVEIESLFDGVDFSEPLTRARFEELNNDLFRKTMGPVKKAMDDAGLQKSQIDEIVLVGGSTRIPKVQQLLKDFFEGKEPNKGVNPDEAVAYGAAVQGGILSGEGGDETKDILLLDVAPLTLGIETVGGVMTKLIPRNTVIPTKKSQVFTTYQDQQTTVSIQVFEGERSLTKDCRLLGKFDLNGIPPAPRGTPQIEVTFEVDANGILNVKAEDKASGKSEKITITNEKGRLSQEEIDRMVKEAEEFAEEDKKVKEKIDARNALETYVYNMKNQVNDKDKLADKLEGDEKEKIEAATKEALEWLDENQNSEKEEYDEKLKEVEAVCNPIITAVYQRSGGAPGGAGGESSTEEEDESHDEL 20F38DA029F3F3F2 q9m0z8_arath AT4g04780 At4g04780 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSTNSYYSSASSSGFRVCPPGVPSKCWCGEEIITFTSKTKENPYRRFYRCAIAMKRENEEHLFKWVDEALLDEIKMVNEKCKRVVENISDLRMNVMANMELLNKNAKQMEEELIKKMEGELLTMKENVEELGHVMAKSALKTVGVAVVIVASIVWLWGRVRFKETTEIVPYEFYGIRAQKSKGKATLKAHFKNLRAQTQNAERSLLRTTKRPNSQRLSVVATPNRLCVAGEILSTGLLRFWKMDIISQLQEQVNTIAAITFNAFGTLQRDAPPVQLSPNYPEPPATTTVTDDATPFPEQPKQLSAGLVKAAKQFDALVAALPLSEGGEGAQLKRIAELQVENDLVGQELQKQLEAAEKELKQVQELFGQAADNCLNMKKPE 71405B7196A07E23 med6-med7 2 4 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 1 C we decided to affinity purify the anti-AtMed6 antibodies to use them for immunoprecipitation of a possible A. thaliana Mediator complex directly from the 200 mM potassium acetate eluate of the DEAE-Sephacel column. Western blot analysis showed that AtMed7 partially coimmunoprecipitated with AtMed6 med4-med6 1 14 unspecified role unspecified role prey prey 41 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 32 unspecified role unspecified role prey prey 29 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 38 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 33 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 21 unspecified role unspecified role prey prey 27 unspecified role unspecified role prey prey 40 unspecified role unspecified role prey prey 39 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 28 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 42 unspecified role unspecified role prey prey 35 unspecified role unspecified role prey prey 25 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 36 unspecified role unspecified role prey prey 30 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 31 unspecified role unspecified role prey prey 34 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait 26 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 37 unspecified role unspecified role prey prey association association Table 1 Table 2 Mediator and RNA Polymerase Subunits of Arabidopsis thaliana Identified by LC-ESI-MS/MS at1g11760-med6-med7 2 4 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 20 unspecified role unspecified role bait bait association association 4C In order to further characterize the A. thaliana Mediator, the α-At1g11760 antibodies were affinity purified and used for immunoprecipitation experiments using the 200 mM potassium acetate eluate from the DEAE-Sephacel column. Figure 4C shows that both AtMed6 and AtMed7 are coimmunoprecipitated with At1g11760.