IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The 20S proteasome of Schistosoma mansoni: a proteomic analysis. The 20S proteasome of Schistosoma mansoni: a proteomic analysis. Proteomics (1615-9853) 2007 imex curation Castro-Borges W., Cartwright J., Ashton PD., Braschi S., Guerra Sa R., Rodrigues V., Wilson RA., Curwen RS. Adult worm extracts were treated to remove lipid and nucleic acids. The treated extract was loaded on to a high capacity anion exchange column and chymotrypsin activity measured for each fraction from the column, concentrated and loaded onto a Phenyl sepharose column, prior to SDS-PAGE separation. wb5@york.ac.uk 07-Sept-2007: Contacted by JYOTI. orchard Only protein-protein interactions 1 schma Schistosoma mansoni (Blood fluke) Blood fluke ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom fingerprinting peptide massfingerprinting fingerprinting Castro-Borges W., Cartwright J., Ashton PD., Braschi S., Guerra Sa R., Rodrigues V., Wilson RA., Curwen RS. Adult worm extracts were treated to remove lipid and nucleic acids. The treated extract was loaded on to a high capacity anion exchange column and chymotrypsin activity measured for each fraction from the column, concentrated and loaded onto a Phenyl sepharose column, prior to SDS-PAGE separation. wb5@york.ac.uk Accepted 2007-SEP-31 by ORCHARD. 07-Sept-2007: Contacted by JYOTI. Proteomics (1615-9853) 2007 orchard Only protein-protein interactions 1 imex curation g4vsw3_schma Proteasome subunit beta protein protein schma Schistosoma mansoni (Blood fluke) Blood fluke MSSLVVDRRSGGFSFENCYRNKLIEQKGFELPKAVKTGTTICGVVFKNGVVLGADTRATEGTVVAEKNCSKIHRISENIYCCGAGTAADTQMVTLMISSQVELHSLNTGRTPRVIAPLRLLKRYLYQYQGYVGAALVLGGVDSTGPHLYSIAPHGSTDKLPYITMGSGSLACMSVLESRFKFDMEQDEAVKLVRDGIAAGIFNDMGSGSNVDICIITKDGTTYIRSYDEANVKGKRAEKYNPPEGTTSVLHKSVHHVEFDVVTTRVVRDIPAHSVETMDLS F788E2F82371339A g4v926_schma Proteasome endopeptidase complex protein protein schma Schistosoma mansoni (Blood fluke) Blood fluke MLSVLNANQKKLNDFTDDTKGISTGTTLFACEYDGGVVIGADSRTSSGTYVVNRVTDKLTQLTKSIYCCRSGSAADTQAVADMVRYQLDFHRLEMNREPTVLEAAVSCKHFCYNYRDDLVAGIIVAGWDEELGGQIYSIPLGGMLIRQPIVIGGSGSTYTYGYVDHGFRKGMTREECVSFVLKGVALAINRDGSSGGCVRLAIISKDGVERILTKGDEVPVYRFS BEF5633EE06EE447 g4vdg3_schma Proteasome subunit alpha type Smp_032580.2 protein protein schma Schistosoma mansoni (Blood fluke) Blood fluke MFLTRTEYDRGVNTFSPEGRLFQVEYAIEATKLGSTGIGIKTSEGVVMAVEKRVNSTLIIPSSIEKIFEVDKHIACAVSGLVADARTLIERARTEAAHHWFVYNEKMTIEDVTKAVSNLALAFGDDDMESGAMSRPFGVALLFAGVDERGPQLYHMDPSGTYIRYEAKAIGSGSEGAQQALQEIYHKNMTLHEGCKHALSILKQVMEEKLDSTNVEMATVSIKDNYHLFNKDEVQKIIEEINQSSS The sequence has been withdrawn from uniprot. 7944EA038ACEA24F g4vbu6_schma Proteasome endopeptidase complex Smp_207080 peptide peptide polypeptide oligopeptide oligopeptide polypeptide schma Schistosoma mansoni (Blood fluke) Blood fluke MMSASTPSGFDRFMTIFSPEGRLYQVEYTFKAISLDGHLSVGVRGNDCAVVAAQLKLPDKLIDRRSMTRIFRLTDSTGCIMTGMAPDCRAQVQRARYEAASFKHKFGYEVPCDVLLHRIGEINQVYTQSAEMRPLGCAMLAISYDEELGKPQLYKSDPSGFVAGHRAVAVGDKQTEAMRHLENEFRKQENYTLEEAVELAIGCLSHTHSMDFKATELEIAVVSKADPIFR The sequence has been withdrawn from uniprot. 55B191A041D12550 g4vaq6_schma Proteasome subunit beta protein protein schma Schistosoma mansoni (Blood fluke) Blood fluke MFNPYSSNGGTVMGIAGNDFSLIASDTRLCDGDFVILSRNSPRLFKLGPGNILGCAGFHGDVLTLTKLLENKVKTFRYDHGKNISTKALAGLLSVTLYNRRFFPFYVSNVLVGLDEGRGVLYSYDPVGSYESEGYRASGSSSAILQPFLDSTVGNKTSKYHVSRLDKETAVKLTHDIFISAAERDIQCGDSVIICTITKDGLSEVSYPLRRD 3B7AFEB921E904E2 g4vrc3_schma Proteasome subunit beta Smp_074500.2 protein protein schma Schistosoma mansoni (Blood fluke) Blood fluke MECIIGIKMNDFVLLAADMRCAHSILTIKHDELKMFKFSKRVLAAVCGESGDTSQFSEFIQQNMQLYEIRNGYELTPSAAANFTRNNMAESLRSRSPYFVNLLIAGFDLQTGPELYYLDYLASLIKVPFAVHGYGALVALSILDRMHRPDMTVNEAVALLRLCIQEVQKRLVINLDRYMVRIVTKDGIEELPDLTDFAVKE The sequence has been withdrawn from uniprot. 170FA6C0872BC850 g4m183_schma Proteasome subunit alpha type protein protein schma Schistosoma mansoni (Blood fluke) Blood fluke MARRYDTRTTIFSPEGRLYQVEYAMEAVGHAGTCLGVVAKDGIVLAAEKRFINNLLDETVFSEKIYKINDDIACAVAGITADATVLINEMRLIAQRYLLSYQEPMPVEQLVCHICDLQHGYTMYGGRRPFGVSMLFIGWDKRYGYQLYQTDPSGNFLGWNATCIGSNSTAAGSILEQDYNADATVEEATKLCIKVLYKTMTMSKLTSEKVEIGVLQRRNEKTYVRLINQSQVDTLIKTVEGEETQKK 48A59B6F96F4452D g4vhx9_schma Proteasome subunit alpha type-3 protein protein schma Schistosoma mansoni (Blood fluke) Blood fluke MSSIGSGYDLTASQFSRGGNVFQIEYACKAVENSGTAIAIQGKDCVVFAVENIVTSKLYEPGCVKRIYNIDDHIGMVVMGFQTDAKAIVEAAREECANYRNSFGSRIPLYSLTERLAMYMHAHTLYSAVRPFGVSVLLGSYENDGPHLFVIEPSGLSYAYFGCAIGKAKQNATTEIEKLKMNDLSPEELIKEAAKIIYTVHDEIKDKNFELDLSWVGANTKGIHQPVPRKLFDEAETFAKQALEEADDLDDEVE 5B38EF5AFF9D6A74 g4lz69_schma Proteasome subunit alpha type protein protein schma Schistosoma mansoni (Blood fluke) Blood fluke MSTKYDRAITIFSPDGHLFQVEYAQEAVKKGSTAIGVRGKDCVVLCVEKKATTKLQNDRTVRKIALLDDHVAVAFAGLTADARILISRIRVECKSYKLTVEDPVSLEYIARYMAQLKQKYTQSNGRRPFGVSTLICGFNQDGTPHLYQTDPSGTHFEWLANAIGKNAKVAREFLEKNYTEDAVADEYGTVKLAVKALMEVVQSGAKYMELAVMRWKAPSKPGEPFVDWQMLEFADIQRYVQAIEKEREEEAEKKRQKKEAAAAL 39FC9004E9A5F385 g4vp46_schma Proteasome endopeptidase complex Smp_164840 protein protein schma Schistosoma mansoni (Blood fluke) Blood fluke MALLDITGFEMVNGLEKGPGARSVCTHLTEVDLYRSQKAENYLKPIEYGIQCKETKINFFHGTTTLALKYSGGVIVATDSRATAGPYIASGTTKKIIEINKYLLGTMAGGAADCQFWERVLAKHCRLFELRNKERISVAAASKLLANIVYNYKGMGLSIGTTIVGWDKNGSGIYYVDTDGNRTPGNLFSVGSGSPYAYGVLDTGYKYEMSDEDAYELARRSIFHATHRDAASGGFVNPQFDLPRFRQWDNITGLHTSGMSIRDWQMKRAFQASLYLHNPNAVIILGDILDEGKWATNDDFNYLVERFRDIFHHDKTKTLVKTVVGNHDIGFHYATNEFLNHRFHRDVGDNVYTPPIYLWSFFGIHFVIANSIAFEGDNCDLCFKANFILRLIARYLDCLKLSTPSNAKNPSSFVYSRPVILQHFPLYRSSDRGCSTKPIDAMPKHLRKTVNRPKLDCLSKEATKQLLESLRPRLILSGHTHYSCKMSHQFGNQSDTAVEWSVASFSWRNLANPGFLMV The sequence has been withdrawn from uniprot. BFBDF0384F851892 g4luj6_schma Proteasome subunit beta protein protein schma Schistosoma mansoni (Blood fluke) Blood fluke MSILAHNGSAVIAMSGKDCAAIACDLRFGVNLQTISTDFTKVYPLGPRLYVGFPGLATDNQTVFQRLQFRKNMYELRENKTIKPQTITTMLSNLLYERRFGPYFVEPVIAGIDHTTNKPYVACMDLIGCVCEANDFVVSGTCTEQMYGMCETLWEENMNSKELFECISQCILNALNRDAVSGWGARVYLLEKDAVTISDLKMRMD 50A2525AB92903FC g4vhl4_schma Proteasome endopeptidase complex Smp_170730 protein protein schma Schistosoma mansoni (Blood fluke) Blood fluke MFRNQYDHDVSIWSPQGRIHQIEYAMEAVKQGSAAVALKNHDYAIIVALKRAPSELSSYQEKIIQIDDHVGVAISGLTADGRLLSRSMRRECADSRWAYDEPLPISRLLSKIALKMQVPTQRYGRRPFGVGMLVTGCDALGPHVYYLCPSSNAYDCKCIAIGSRSQSARTYLERHLNEFKGSSLDDLICHGLKALNSTLPNEVSLNIKNCSLAIVGKDMKFTIFEDDDIDPYLKLFEATQVDAPAAAIPDSGPTPMEQDQSALLCDIQLACVKLAELKAYVTSTQFCNWHFTPSELITLRSDCNATALKRLQSTKEVEVRNNNNENMNNTLVVGLDPDQEFKIVKHYVYLMKILFDKFTTPQLPNEVYGFAATYFKRFYLNHSVMDFYPRDIMLTCLYVACKAADFPIGLQTFISHIPRNQERYSYLVLNSELFLMESLGYDLWVYTPYQALTGFVIDLVAYQRRICGAHSDSYLLENRSDAQLVDELCKDGVNLINLWYQTDLCLTAHPSQFALAVLVELGHTRPQLDVEVFVRDELCGCDPVQKFKQHSEEEDGDESDTKPKKDTKCDPETRWQELSSRLDFIRQTINEFQFVTDLGPVGEEEVILDECRNPLYNIESDEYAQAKSKADSLMAMLE The sequence has been withdrawn from uniprot. 104CB626D4B0A097 g4lye6_schma Proteasome subunit beta protein protein schma Schistosoma mansoni (Blood fluke) Blood fluke MDSLQVKFPKSSTDVHGDYKQHTMTPICTGTSVIGIKYRNGVVLAADMLVSYGSLAKYMDFERMFKVNQSTVMCCSGDVADFQFLKHHIEELTHQDSLLSDGFRLSPHALHSWITRVLYNRRSRLQPLWNTCLVGGLEANGEPFIGYCSMLGVSFTENYVATGFGAYLAMPILRECLEIKAGGDPNNITEEDAISTMTSAMKQLYFRDCRAFDTYQMAIVNKEGIQINGPLRLKCDWSIAEYVKGYD 594F38F9329DC359 g4lzy3_schma Proteasome subunit alpha type Smp_067890 protein protein schma Schistosoma mansoni (Blood fluke) Blood fluke MSERYSFSLTTFSPSGKLVQIEYALKAVEAGAPSVGIRAANGVVLAAVKKFTSKLMDESTVTKIEQVTKGIGMVYSGLSPDYRVLVKQARKSSQAYQLAYGEPISPEQLVIRIAAVMQEYTQSGGVRPFGVSLLVAGWDRDLQRPFLYQCDPSGTYFPWKATALGQNSQNGKSFLEKRNILFLFNYSSCCFTIKIQKKLILSVSTLFYNN The sequence has been withdrawn from uniprot. E2BCB07E3A5ED135 sm01587-sm12504 1 4 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component 2 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component physical association physical association Table 2 Mass spectrometric data for the identified α and β 20S proteasome subunits from S.mansoni. None of the proteins mapped to a UniProt accession number.