IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A role for the HOXB7 homeodomain protein in DNA repair. A role for the HOXB7 homeodomain protein in DNA repair. Cancer Res. (0008-5472) 2007 Rubin E., Wu X., Zhu T., Cheung JC., Chen H., Lorincz A., Pandita RK., Sharma GG., Ha HC., Gasson J., Hanakahi LA., Pandita TK., Sukumar S. Cancer - Interactions investigated in the context of cancer Cell extracts from MCF10A were fractionated by affinity chromatography on GST-HOXB7-Sepharose. Eluates were subjected to SDS-PAGE and both Coomassie and Silver staining. To precisely identify the eluted proteins, direct sequencing from PVDF membranes identified Ku80 from an N-terminal sequence, for the 100 and 70 kDa bands, peptide mass fingerprints were obtained by MALDI-TOF. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. saras@jhmi.edu 01-Feb-2008: Contacted by JYOTI. orchard crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Rubin E., Wu X., Zhu T., Cheung JC., Chen H., Lorincz A., Pandita RK., Sharma GG., Ha HC., Gasson J., Hanakahi LA., Pandita TK., Sukumar S. Cancer - Interactions investigated in the context of cancer Cell extracts from MCF10A were fractionated by affinity chromatography on GST-HOXB7-Sepharose. Eluates were subjected to SDS-PAGE and both Coomassie and Silver staining. To precisely identify the eluted proteins, direct sequencing from PVDF membranes identified Ku80 from an N-terminal sequence, for the 100 and 70 kDa bands, peptide mass fingerprints were obtained by MALDI-TOF. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. saras@jhmi.edu Accepted 2008-FEB-8 by ORCHARD. 01-Feb-2008: Contacted by JYOTI. Cancer Res. (0008-5472) 2007 orchard A role for the HOXB7 homeodomain protein in DNA repair. A role for the HOXB7 homeodomain protein in DNA repair. Cancer Res. (0008-5472) 2007 Rubin E., Wu X., Zhu T., Cheung JC., Chen H., Lorincz A., Pandita RK., Sharma GG., Ha HC., Gasson J., Hanakahi LA., Pandita TK., Sukumar S. Cancer - Interactions investigated in the context of cancer Cell extracts from MCF10A were fractionated by affinity chromatography on GST-HOXB7-Sepharose. Eluates were subjected to SDS-PAGE and both Coomassie and Silver staining. To precisely identify the eluted proteins, direct sequencing from PVDF membranes identified Ku80 from an N-terminal sequence, for the 100 and 70 kDa bands, peptide mass fingerprints were obtained by MALDI-TOF. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. saras@jhmi.edu 01-Feb-2008: Contacted by JYOTI. orchard in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Rubin E., Wu X., Zhu T., Cheung JC., Chen H., Lorincz A., Pandita RK., Sharma GG., Ha HC., Gasson J., Hanakahi LA., Pandita TK., Sukumar S. Cancer - Interactions investigated in the context of cancer Cell extracts from MCF10A were fractionated by affinity chromatography on GST-HOXB7-Sepharose. Eluates were subjected to SDS-PAGE and both Coomassie and Silver staining. To precisely identify the eluted proteins, direct sequencing from PVDF membranes identified Ku80 from an N-terminal sequence, for the 100 and 70 kDa bands, peptide mass fingerprints were obtained by MALDI-TOF. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. saras@jhmi.edu Accepted 2008-FEB-8 by ORCHARD. 01-Feb-2008: Contacted by JYOTI. Cancer Res. (0008-5472) 2007 orchard A role for the HOXB7 homeodomain protein in DNA repair. A role for the HOXB7 homeodomain protein in DNA repair. Cancer Res. (0008-5472) 2007 Rubin E., Wu X., Zhu T., Cheung JC., Chen H., Lorincz A., Pandita RK., Sharma GG., Ha HC., Gasson J., Hanakahi LA., Pandita TK., Sukumar S. Cancer - Interactions investigated in the context of cancer Cell extracts from MCF10A were fractionated by affinity chromatography on GST-HOXB7-Sepharose. Eluates were subjected to SDS-PAGE and both Coomassie and Silver staining. To precisely identify the eluted proteins, direct sequencing from PVDF membranes identified Ku80 from an N-terminal sequence, for the 100 and 70 kDa bands, peptide mass fingerprints were obtained by MALDI-TOF. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. saras@jhmi.edu 01-Feb-2008: Contacted by JYOTI. orchard human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Rubin E., Wu X., Zhu T., Cheung JC., Chen H., Lorincz A., Pandita RK., Sharma GG., Ha HC., Gasson J., Hanakahi LA., Pandita TK., Sukumar S. Cancer - Interactions investigated in the context of cancer Cell extracts from MCF10A were fractionated by affinity chromatography on GST-HOXB7-Sepharose. Eluates were subjected to SDS-PAGE and both Coomassie and Silver staining. To precisely identify the eluted proteins, direct sequencing from PVDF membranes identified Ku80 from an N-terminal sequence, for the 100 and 70 kDa bands, peptide mass fingerprints were obtained by MALDI-TOF. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. saras@jhmi.edu Accepted 2008-FEB-8 by ORCHARD. 01-Feb-2008: Contacted by JYOTI. Cancer Res. (0008-5472) 2007 orchard A role for the HOXB7 homeodomain protein in DNA repair. A role for the HOXB7 homeodomain protein in DNA repair. Cancer Res. (0008-5472) 2007 Rubin E., Wu X., Zhu T., Cheung JC., Chen H., Lorincz A., Pandita RK., Sharma GG., Ha HC., Gasson J., Hanakahi LA., Pandita TK., Sukumar S. Cancer - Interactions investigated in the context of cancer Cell extracts from MCF10A were fractionated by affinity chromatography on GST-HOXB7-Sepharose. Eluates were subjected to SDS-PAGE and both Coomassie and Silver staining. To precisely identify the eluted proteins, direct sequencing from PVDF membranes identified Ku80 from an N-terminal sequence, for the 100 and 70 kDa bands, peptide mass fingerprints were obtained by MALDI-TOF. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. saras@jhmi.edu 01-Feb-2008: Contacted by JYOTI. orchard in vitro In vitro pull down pull down protein sequence protein sequence identification protein sequence Rubin E., Wu X., Zhu T., Cheung JC., Chen H., Lorincz A., Pandita RK., Sharma GG., Ha HC., Gasson J., Hanakahi LA., Pandita TK., Sukumar S. Cancer - Interactions investigated in the context of cancer Cell extracts from MCF10A were fractionated by affinity chromatography on GST-HOXB7-Sepharose. Eluates were subjected to SDS-PAGE and both Coomassie and Silver staining. To precisely identify the eluted proteins, direct sequencing from PVDF membranes identified Ku80 from an N-terminal sequence, for the 100 and 70 kDa bands, peptide mass fingerprints were obtained by MALDI-TOF. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. saras@jhmi.edu Accepted 2008-FEB-8 by ORCHARD. 01-Feb-2008: Contacted by JYOTI. Cancer Res. (0008-5472) 2007 orchard A role for the HOXB7 homeodomain protein in DNA repair. A role for the HOXB7 homeodomain protein in DNA repair. Cancer Res. (0008-5472) 2007 Rubin E., Wu X., Zhu T., Cheung JC., Chen H., Lorincz A., Pandita RK., Sharma GG., Ha HC., Gasson J., Hanakahi LA., Pandita TK., Sukumar S. Cancer - Interactions investigated in the context of cancer Cell extracts from MCF10A were fractionated by affinity chromatography on GST-HOXB7-Sepharose. Eluates were subjected to SDS-PAGE and both Coomassie and Silver staining. To precisely identify the eluted proteins, direct sequencing from PVDF membranes identified Ku80 from an N-terminal sequence, for the 100 and 70 kDa bands, peptide mass fingerprints were obtained by MALDI-TOF. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. saras@jhmi.edu 01-Feb-2008: Contacted by JYOTI. orchard human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Rubin E., Wu X., Zhu T., Cheung JC., Chen H., Lorincz A., Pandita RK., Sharma GG., Ha HC., Gasson J., Hanakahi LA., Pandita TK., Sukumar S. Cancer - Interactions investigated in the context of cancer Cell extracts from MCF10A were fractionated by affinity chromatography on GST-HOXB7-Sepharose. Eluates were subjected to SDS-PAGE and both Coomassie and Silver staining. To precisely identify the eluted proteins, direct sequencing from PVDF membranes identified Ku80 from an N-terminal sequence, for the 100 and 70 kDa bands, peptide mass fingerprints were obtained by MALDI-TOF. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. saras@jhmi.edu Accepted 2008-FEB-8 by ORCHARD. 01-Feb-2008: Contacted by JYOTI. Cancer Res. (0008-5472) 2007 orchard A role for the HOXB7 homeodomain protein in DNA repair. A role for the HOXB7 homeodomain protein in DNA repair. Cancer Res. (0008-5472) 2007 Rubin E., Wu X., Zhu T., Cheung JC., Chen H., Lorincz A., Pandita RK., Sharma GG., Ha HC., Gasson J., Hanakahi LA., Pandita TK., Sukumar S. Cancer - Interactions investigated in the context of cancer Cell extracts from MCF10A were fractionated by affinity chromatography on GST-HOXB7-Sepharose. Eluates were subjected to SDS-PAGE and both Coomassie and Silver staining. To precisely identify the eluted proteins, direct sequencing from PVDF membranes identified Ku80 from an N-terminal sequence, for the 100 and 70 kDa bands, peptide mass fingerprints were obtained by MALDI-TOF. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. saras@jhmi.edu 01-Feb-2008: Contacted by JYOTI. orchard human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Rubin E., Wu X., Zhu T., Cheung JC., Chen H., Lorincz A., Pandita RK., Sharma GG., Ha HC., Gasson J., Hanakahi LA., Pandita TK., Sukumar S. Cancer - Interactions investigated in the context of cancer Cell extracts from MCF10A were fractionated by affinity chromatography on GST-HOXB7-Sepharose. Eluates were subjected to SDS-PAGE and both Coomassie and Silver staining. To precisely identify the eluted proteins, direct sequencing from PVDF membranes identified Ku80 from an N-terminal sequence, for the 100 and 70 kDa bands, peptide mass fingerprints were obtained by MALDI-TOF. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. A unique UniProtKB entry could not be associated with PARP, since the exact gene product of the interactor could not be ascertained. saras@jhmi.edu Accepted 2008-FEB-8 by ORCHARD. 01-Feb-2008: Contacted by JYOTI. Cancer Res. (0008-5472) 2007 orchard prkdc_human DNA-dependent protein kinase catalytic subunit PRKDC HYRC HYRC1 DNPK1 p460 protein protein human Homo sapiens Human MAGSGAGVRCSLLRLQETLSAADRCGAALAGHQLIRGLGQECVLSSSPAVLALQTSLVFSRDFGLLVFVRKSLNSIEFRECREEILKFLCIFLEKMGQKIAPYSVEIKNTCTSVYTKDRAAKCKIPALDLLIKLLQTFRSSRLMDEFKIGELFSKFYGELALKKKIPDTVLEKVYELLGLLGEVHPSEMINNAENLFRAFLGELKTQMTSAVREPKLPVLAGCLKGLSSLLCNFTKSMEEDPQTSREIFNFVLKAIRPQIDLKRYAVPSAGLRLFALHASQFSTCLLDNYVSLFEVLLKWCAHTNVELKKAALSALESFLKQVSNMVAKNAEMHKNKLQYFMEQFYGIIRNVDSNNKELSIAIRGYGLFAGPCKVINAKDVDFMYVELIQRCKQMFLTQTDTGDDRVYQMPSFLQSVASVLLYLDTVPEVYTPVLEHLVVMQIDSFPQYSPKMQLVCCRAIVKVFLALAAKGPVLRNCISTVVHQGLIRICSKPVVLPKGPESESEDHRASGEVRTGKWKVPTYKDYVDLFRHLLSSDQMMDSILADEAFFSVNSSSESLNHLLYDEFVKSVLKIVEKLDLTLEIQTVGEQENGDEAPGVWMIPTSDPAANLHPAKPKDFSAFINLVEFCREILPEKQAEFFEPWVYSFSYELILQSTRLPLISGFYKLLSITVRNAKKIKYFEGVSPKSLKHSPEDPEKYSCFALFVKFGKEVAVKMKQYKDELLASCLTFLLSLPHNIIELDVRAYVPALQMAFKLGLSYTPLAEVGLNALEEWSIYIDRHVMQPYYKDILPCLDGYLKTSALSDETKNNWEVSALSRAAQKGFNKVVLKHLKKTKNLSSNEAISLEEIRIRVVQMLGSLGGQINKNLLTVTSSDEMMKSYVAWDREKRLSFAVPFREMKPVIFLDVFLPRVTELALTASDRQTKVAACELLHSMVMFMLGKATQMPEGGQGAPPMYQLYKRTFPVLLRLACDVDQVTRQLYEPLVMQLIHWFTNNKKFESQDTVALLEAILDGIVDPVDSTLRDFCGRCIREFLKWSIKQITPQQQEKSPVNTKSLFKRLYSLALHPNAFKRLGASLAFNNIYREFREEESLVEQFVFEALVIYMESLALAHADEKSLGTIQQCCDAIDHLCRIIEKKHVSLNKAKKRRLPRGFPPSASLCLLDLVKWLLAHCGRPQTECRHKSIELFYKFVPLLPGNRSPNLWLKDVLKEEGVSFLINTFEGGGCGQPSGILAQPTLLYLRGPFSLQATLCWLDLLLAALECYNTFIGERTVGALQVLGTEAQSSLLKAVAFFLESIAMHDIIAAEKCFGTGAAGNRTSPQEGERYNYSKCTVVVRIMEFTTTLLNTSPEGWKLLKKDLCNTHLMRVLVQTLCEPASIGFNIGDVQVMAHLPDVCVNLMKALKMSPYKDILETHLREKITAQSIEELCAVNLYGPDAQVDRSRLAAVVSACKQLHRAGLLHNILPSQSTDLHHSVGTELLSLVYKGIAPGDERQCLPSLDLSCKQLASGLLELAFAFGGLCERLVSLLLNPAVLSTASLGSSQGSVIHFSHGEYFYSLFSETINTELLKNLDLAVLELMQSSVDNTKMVSAVLNGMLDQSFRERANQKHQGLKLATTILQHWKKCDSWWAKDSPLETKMAVLALLAKILQIDSSVSFNTSHGSFPEVFTTYISLLADTKLDLHLKGQAVTLLPFFTSLTGGSLEELRRVLEQLIVAHFPMQSREFPPGTPRFNNYVDCMKKFLDALELSQSPMLLELMTEVLCREQQHVMEELFQSSFRRIARRGSCVTQVGLLESVYEMFRKDDPRLSFTRQSFVDRSLLTLLWHCSLDALREFFSTIVVDAIDVLKSRFTKLNESTFDTQITKKMGYYKILDVMYSRLPKDDVHAKESKINQVFHGSCITEGNELTKTLIKLCYDAFTENMAGENQLLERRRLYHCAAYNCAISVICCVFNELKFYQGFLFSEKPEKNLLIFENLIDLKRRYNFPVEVEVPMERKKKYIEIRKEAREAANGDSDGPSYMSSLSYLADSTLSEEMSQFDFSTGVQSYSYSSQDPRPATGRFRRREQRDPTVHDDVLELEMDELNRHECMAPLTALVKHMHRSLGPPQGEEDSVPRDLPSWMKFLHGKLGNPIVPLNIRLFLAKLVINTEEVFRPYAKHWLSPLLQLAASENNGGEGIHYMVVEIVATILSWTGLATPTGVPKDEVLANRLLNFLMKHVFHPKRAVFRHNLEIIKTLVECWKDCLSIPYRLIFEKFSGKDPNSKDNSVGIQLLGIVMANDLPPYDPQCGIQSSEYFQALVNNMSFVRYKEVYAAAAEVLGLILRYVMERKNILEESLCELVAKQLKQHQNTMEDKFIVCLNKVTKSFPPLADRFMNAVFFLLPKFHGVLKTLCLEVVLCRVEGMTELYFQLKSKDFVQVMRHRDDERQKVCLDIIYKMMPKLKPVELRELLNPVVEFVSHPSTTCREQMYNILMWIHDNYRDPESETDNDSQEIFKLAKDVLIQGLIDENPGLQLIIRNFWSHETRLPSNTLDRLLALNSLYSPKIEVHFLSLATNFLLEMTSMSPDYPNPMFEHPLSECEFQEYTIDSDWRFRSTVLTPMFVETQASQGTLQTRTQEGSLSARWPVAGQIRATQQQHDFTLTQTADGRSSFDWLTGSSTDPLVDHTSPSSDSLLFAHKRSERLQRAPLKSVGPDFGKKRLGLPGDEVDNKVKGAAGRTDLLRLRRRFMRDQEKLSLMYARKGVAEQKREKEIKSELKMKQDAQVVLYRSYRHGDLPDIQIKHSSLITPLQAVAQRDPIIAKQLFSSLFSGILKEMDKFKTLSEKNNITQKLLQDFNRFLNTTFSFFPPFVSCIQDISCQHAALLSLDPAAVSAGCLASLQQPVGIRLLEEALLRLLPAELPAKRVRGKARLPPDVLRWVELAKLYRSIGEYDVLRGIFTSEIGTKQITQSALLAEARSDYSEAAKQYDEALNKQDWVDGEPTEAEKDFWELASLDCYNHLAEWKSLEYCSTASIDSENPPDLNKIWSEPFYQETYLPYMIRSKLKLLLQGEADQSLLTFIDKAMHGELQKAILELHYSQELSLLYLLQDDVDRAKYYIQNGIQSFMQNYSSIDVLLHQSRLTKLQSVQALTEIQEFISFISKQGNLSSQVPLKRLLNTWTNRYPDAKMDPMNIWDDIITNRCFFLSKIEEKLTPLPEDNSMNVDQDGDPSDRMEVQEQEEDISSLIRSCKFSMKMKMIDSARKQNNFSLAMKLLKELHKESKTRDDWLVSWVQSYCRLSHCRSRSQGCSEQVLTVLKTVSLLDENNVSSYLSKNILAFRDQNILLGTTYRIIANALSSEPACLAEIEEDKARRILELSGSSSEDSEKVIAGLYQRAFQHLSEAVQAAEEEAQPPSWSCGPAAGVIDAYMTLADFCDQQLRKEEENASVIDSAELQAYPALVVEKMLKALKLNSNEARLKFPRLLQIIERYPEETLSLMTKEISSVPCWQFISWISHMVALLDKDQAVAVQHSVEEITDNYPQAIVYPFIISSESYSFKDTSTGHKNKEFVARIKSKLDQGGVIQDFINALDQLSNPELLFKDWSNDVRAELAKTPVNKKNIEKMYERMYAALGDPKAPGLGAFRRKFIQTFGKEFDKHFGKGGSKLLRMKLSDFNDITNMLLLKMNKDSKPPGNLKECSPWMSDFKVEFLRNELEIPGQYDGRGKPLPEYHVRIAGFDERVTVMASLRRPKRIIIRGHDEREHPFLVKGGEDLRQDQRVEQLFQVMNGILAQDSACSQRALQLRTYSVVPMTSRLGLIEWLENTVTLKDLLLNTMSQEEKAAYLSDPRAPPCEYKDWLTKMSGKHDVGAYMLMYKGANRTETVTSFRKRESKVPADLLKRAFVRMSTSPEAFLALRSHFASSHALICISHWILGIGDRHLNNFMVAMETGGVIGIDFGHAFGSATQFLPVPELMPFRLTRQFINLMLPMKETGLMYSIMVHALRAFRSDPGLLTNTMDVFVKEPSFDWKNFEQKMLKKGGSWIQEINVAEKNWYPRQKICYAKRKLAGANPAVITCDELLLGHEKAPAFRDYVAVARGSKDHNIRAQEPESGLSEETQVKCLMDQATDPNILGRTWEGWEPWM DIP protein P78527 original sequence version: 118 DIP protein P78527-1 original sequence version: 117 DIP protein P78527 original sequence version: 170 DIP protein P78527 original sequence version: 179 DIP protein P78527 original sequence version: 114 DIP protein P78527 original sequence version: 125 AC6E747FEB09F3E5 xrcc5_human X-ray repair cross-complementing protein 5 XRCC5 G22P2 ATP-dependent DNA helicase II 80 kDa subunit Lupus Ku autoantigen protein p86 86 kDa subunit of Ku antigen Ku86 Ku80 Thyroid-lupus autoantigen CTC box-binding factor 85 kDa subunit Nuclear factor IV DNA repair protein XRCC5 X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) ATP-dependent DNA helicase 2 subunit 2 protein protein human Homo sapiens Human MVRSGNKAAVVLCMDVGFTMSNSIPGIESPFEQAKKVITMFVQRQVFAENKDEIALVLFGTDGTDNPLSGGDQYQNITVHRHLMLPDFDLLEDIESKIQPGSQQADFLDALIVSMDVIQHETIGKKFEKRHIEIFTDLSSRFSKSQLDIIIHSLKKCDISLQFFLPFSLGKEDGSGDRGDGPFRLGGHGPSFPLKGITEQQKEGLEIVKMVMISLEGEDGLDEIYSFSESLRKLCVFKKIERHSIHWPCRLTIGSNLSIRIAAYKSILQERVKKTWTVVDAKTLKKEDIQKETVYCLNDDDETEVLKEDIIQGFRYGSDIVPFSKVDEEQMKYKSEGKCFSVLGFCKSSQVQRRFFMGNQVLKVFAARDDEAAAVALSSLIHALDDLDMVAIVRYAYDKRANPQVGVAFPHIKHNYECLVYVQLPFMEDLRQYMFSSLKNSKKYAPTEAQLNAVDALIDSMSLAKKDEKTDTLEDLFPTTKIPNPRFQRLFQCLLHRALHPREPLPPIQQHIWNMLNPPAEVTTKSQIPLSKIKTLFPLIEAKKKDQVTAQEIFQDNHEDGPTAKKLKTEQGGAHFSVSSLAEGSVTSVGSVNPAENFRVLVKQKKASFEEASNQLINHIEQFLDTNETPYFMKSIDCIRAFREEAIKFSEEQRFNNFLKALQEKVEIKQLNHFWEIVVQDGITLITKEEASGSSVTAEEAKKFLAPKDKPSGDTAAVFEEGGDVDDLLDMI DIP protein P13010 original sequence version: 128 DIP protein P13010 original sequence version: 127 DIP protein P13010 original sequence version: 122 DIP protein P13010 original sequence version: 174 DIP protein P13010 original sequence version: 153 DIP protein P13010 original sequence version: 155 DIP protein P13010 original sequence version: 142 2363CA84834E74A3 xrcc6_human X-ray repair cross-complementing protein 6 XRCC6 G22P1 ATP-dependent DNA helicase II 70 kDa subunit Lupus Ku autoantigen protein p70 70 kDa subunit of Ku antigen Thyroid-lupus autoantigen CTC box-binding factor 75 kDa subunit DNA repair protein XRCC6 X-ray repair complementing defective repair in Chinese hamster cells 6 ATP-dependent DNA helicase 2 subunit 1 5'-deoxyribose-5-phosphate lyase Ku70 protein protein human Homo sapiens Human MSGWESYYKTEGDEEAEEEQEENLEASGDYKYSGRDSLIFLVDASKAMFESQSEDELTPFDMSIQCIQSVYISKIISSDRDLLAVVFYGTEKDKNSVNFKNIYVLQELDNPGAKRILELDQFKGQQGQKRFQDMMGHGSDYSLSEVLWVCANLFSDVQFKMSHKRIMLFTNEDNPHGNDSAKASRARTKAGDLRDTGIFLDLMHLKKPGGFDISLFYRDIISIAEDEDLRVHFEESSKLEDLLRKVRAKETRKRALSRLKLKLNKDIVISVGIYNLVQKALKPPPIKLYRETNEPVKTKTRTFNTSTGGLLLPSDTKRSQIYGSRQIILEKEETEELKRFDDPGLMLMGFKPLVLLKKHHYLRPSLFVYPEESLVIGSSTLFSALLIKCLEKEVAALCRYTPRRNIPPYFVALVPQEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD DIP protein P12956 original sequence version: 184 DIP protein P12956 original sequence version: 138 DIP protein P12956 original sequence version: 185 DIP protein P12956 original sequence version: 164 DIP protein P12956 original sequence version: 166 BBD3CD434526DFCB hxb7_human Homeobox protein Hox-B7 HOXB7 HOX2C Homeobox protein Hox-2C Homeobox protein HHO.C1 protein protein human Homo sapiens Human MSSLYYANTLFSKYPASSSVFATGAFPEQTSCAFASNPQRPGYGAGSGASFAASMQGLYPGGGGMAGQSAAGVYAAGYGLEPSSFNMHCAPFEQNLSGVCPGDSAKAAGAKEQRDSDLAAESNFRIYPWMRSSGTDRKRGRQTYTRYQTLELEKEFHYNRYLTRRRRIEIAHTLCLTERQIKIWFQNRRMKWKKENKTAGPGTTGQDRAEAEEEEEE E92D63F437D34628 hoxb7-xrcc5-4 4 9 unspecified role unspecified role prey prey human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line 10 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line association association 3 A PARP was also identified as an interactor, but as the isoform of PARP is not clear, it cannot be added to this interaction. DNA-PK (CS) was also inferred as an interactor but definitely identified and thus has not been added as an interactor. hoxb7-xrcc5-3 4 8 unspecified role unspecified role prey prey human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion 10 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion association association 3 A PARP was also identified as an interactor, but as the isoform of PARP is not clear, it cannot be added to this interaction. DNA-PK (CS) was also inferred as an interactor but definitely identified and thus has not been added as an interactor. hoxb7-g22p1-1 2 8 unspecified role unspecified role prey prey human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line 7 unspecified role unspecified role prey prey human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line 10 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line association association 3 B PARP was also identified as an interactor, but the isoform of PARP is not clear and thus it could not be added to the interaction. hoxb7-xrcc5 6 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Immunoprecipitation with GFP antibodies (which also recognize the YFP variant) showed that Ku70 and Ku80 associated with HOXB7 in vivo 3 C P09629 transfection xrcc5-hoxb7 3 10 unspecified role unspecified role prey prey region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait physical association physical association P09629 transfection 3 D Complementary immunoprecipitation using Ku80 (Fig. 3D, lanes 4-6) or DNA-PKcs (Fig. 3D, lanes 7-9) antibodies confirmed the presence of HOXB7-YFP in their complexes prkdc-hoxb7 3 7 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association P09629 transfection 3 D Complementary immunoprecipitation using Ku80 (Fig. 3D, lanes 4-6) or DNA-PKcs (Fig. 3D, lanes 7-9) antibodies confirmed the presence of HOXB7-YFP in their complexes xrcc6-hoxb7 5 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey association association 3 E depletion of DNA in the extracts using ethidium bromide did not reduce the interactions between endogenous HOXB7 and Ku70 or Ku80 The participation of DNA-PK (CS) in this interaction is dependent on the presence of DNA, as it did not interact in the presence of Ethidium Bromide. xrcc5-hoxb7-1 5 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey association association 3 E depletion of DNA in the extracts using ethidium bromide did not reduce the interactions between endogenous HOXB7 and Ku70 or Ku80 The participation of DNA-PK (CS) in this interaction is dependent on the presence of DNA, as it did not interact in the presence of Ethidium Bromide. xrcc6-prkdc 5 9 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey association association 3 E treatment with an intercalating agent (ethidium bromide) effectively blocked the interaction between Ku70/80 and DNA-PK because this reaction was completely dependent on the presence of DNA The participation of DNA-PK (CS) in this interaction is dependent on the presence of DNA, as it did not interact in the presence of Ethidium Bromide. xrcc5-prkdc 5 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey association association 3 E treatment with an intercalating agent (ethidium bromide) effectively blocked the interaction between Ku70/80 and DNA-PK because this reaction was completely dependent on the presence of DNA The participation of DNA-PK (CS) in this interaction is dependent on the presence of DNA, as it did not interact in the presence of Ethidium Bromide. hoxb7-xrcc5-1 1 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 4 A Experiments introducing Fl-HOXB7 into CHO cells (Fig. 4A) showed that coexpression of human Ku70 and human Ku80 was required for the association of either Ku subunit with HOXB7. P09629; P12956; P13010 transfection As coexpression of Ku70 and Ku80 was required for the association of either Ku subunit with HOXB7, it is possible that Ku70/Ku80 heterodimer formation is a prerequisite for HOXB7 binding. hoxb7-xrcc5-2 6 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis range range range range region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey association association 4 B P09629 transfection The results showed that deletion of helix 3 from the homeodomain in HOXB7 (lane 3) completely abolished the interaction between HOXB7 and Ku70/80 proteins.