MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Distinct roles for Mediator Cdk8 module subunits in Drosophila development. Distinct roles for Mediator Cdk8 module subunits in Drosophila development. EMBO J. (0261-4189) 2007 imex curation Loncle N., Boube M., Joulia L., Boschiero C., Werner M., Cribbs DL., Bourbon HM. bourbon@cict.fr orchard Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Loncle N., Boube M., Joulia L., Boschiero C., Werner M., Cribbs DL., Bourbon HM. bourbon@cict.fr Accepted 2007-OCT-27 by ORCHARD. EMBO J. (0261-4189) 2007 orchard Only protein-protein interactions imex curation Distinct roles for Mediator Cdk8 module subunits in Drosophila development. Distinct roles for Mediator Cdk8 module subunits in Drosophila development. EMBO J. (0261-4189) 2007 imex curation Loncle N., Boube M., Joulia L., Boschiero C., Werner M., Cribbs DL., Bourbon HM. bourbon@cict.fr orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Loncle N., Boube M., Joulia L., Boschiero C., Werner M., Cribbs DL., Bourbon HM. bourbon@cict.fr Accepted 2007-OCT-27 by ORCHARD. EMBO J. (0261-4189) 2007 orchard Only protein-protein interactions imex curation ccnc_drome Cyclin-C CycC CLND CG7281 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAGNFWQSSHSQQWILDKPDLLRERQHDLLALNEDEYQKVFIFFANVIQVLGEQLKLRQQVIATATVYFKRFYARNSLKNIDPLLLAPTCILLASKVEEFGVISNSRLISICQSAIKTKFSYAYAQEFPYRTNHILECEFYLLENLDCCLIVYQPYRPLLQLVQDMGQEDQLLTLSWRIVNDSLRTDVCLLYPPYQIAIACLQIACVILQKDATKQWFAELNVDLDKVQEIVRAIVNLYELWKDWKEKDEIQMLLSKIPKPKPPPQR DA73CB7EA89149FE cdk8_drome Cyclin-dependent kinase 8 Cdk8 CG10572 Cell division protein kinase 8 Mediator of RNA polymerase II transcription subunit Cdk8 Mediator complex subunit Cdk8 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDYDFKMKTQIERTKVEDLFNYEGCKVGRGTYGHVYKAKWKETSDGKEYALKQIDGTGLSMSACREIALLRELKHQNVITLIRVFLSHNDRKVFLLIDYAEHDLWHIIKFHRAAKATKKQVVVPRGMVKSLLYQILDGIHYLHSNWVLHRDLKPANILVMGDGNERGRVKIADMGFARLFNAPLKPLADLDPVVVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEDIKTSNPYHHDQLDRIFNVMGFPQDKDWEDIKKMPEHHTLTKDFKRSTYSTCSLAKYMERHKIKPDSKAFHLLQKLLLMDPNKRITSEQAMQDQYFQEEPQPTQDVFAGCPIPYPKREFLTDDDQEDKSDNKRQQQQQQQQQQQQQQQQQQQQQQQQQQQQMNAEPNAKRVRLSGAGNQQDFHHQQQQQQQQQQQQQQQQQQMMFNQQQNFQRFN DIP protein Q9VT57 original sequence version: 95 D73F6219E2A03C98 med12_drome Mediator of RNA polymerase II transcription subunit 12 kto CG8491 Trap230 Mediator complex subunit 12 Mediator complex subunit Kohtalo dTRAP230 Med12 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MLSMLQEKRPLKRTRLGPPDIYPQDAKQREDELTPTNVKHGFTTTPPLSDEFGTAHNSNVNASKVSAFFSGVLAKKEELMTLPDTGRKKQQINCKDNFWPVSPRRKCTVDAWFKDLAGNKPLLSLAKRAPSFNKKEEIFITLCENQVNMQRATWFIKLSAAYTLSFTESKNKKRSIYDPAAEWTGNMIKFMKELLPKLQEYYQQNHDKSSSNGTTSGSLTAAGNGPASNGSTGTSSINSVTGSSASTNVIPVPSMASPLPPIHSPANGQQAAPGGGVNAGSVMPTTGSLGGVVGGPGSSVVGGAAGAGAAVPPGSTISGIGSQFEDSRNALKYWKYCHQLSKYMYEESLLDRQEFLNWILDLLDKMRTQASFDEPLKKLVLSFALQYMHDFVQSERLCRKMAYIVSKKLAQLLNTVVEQQTIKELDEPKLQQDPYELALQEQMSCPHHRDIVLYLSTILQIITIECPTALVWSGIAAHRAPSSLLGSPLDHLPLAPSVLPMPTRCPRTNHEIRRQLRAAESDIVLRTQHAEQRWFAAKWLSAGKNQYTSVLATLDHLDTHCFDRMEHNNSIDTLYAQIFPSPTVSRRREEDQVEPRPPYEPKQDKDTVRILCEWAVSGQRWGEHRAMVVAILLDKRQIDVTSTPADQQSSDKDDKDSLASGAGLIDGLPVFQHVLMHFLDHDAPVLDEHVSSPQQRTEFTNLVQLFSALIRHDVFSHNAYMHTLISRGDLLLESVLVIKSGTTATKTSPPPPAPPPTTTHGFDDDGFGGGLDFKHNEFDDSNVDDDLDKLVQNIKEKGQQHEAPDSPKIGPPGDGETNPGGSISRHYVYTKHFPIPQDDPSMSSYSSESNQRYILLFGVGKERDEKKHAVKKMSKEIGKLFTKKFSIDVAAAGHVKKHSRNEFNFEATTSKCQQMAYFDQHVVTAQCAANVLEQLNGFALGNNNYLPVQEHVAFLFDLMELALNIYSLLELCDSLLKELPEVEHQLQLKKSNLVRSYTTSLALYIVSILRRYHSCLLLSPEQTLSVFEGVCRTIRHVSNPSECTSAERCIIAYLSDLHESCVLLQGKEQSTEYYQQLQCIKRFKDIFNTPEQLDLPPQGYNPLLLQELFMAPRRGGKLDPHWLGTLHESPANVYSFVSNALIAVCRETDNERLNDVALACAELTASCNVLSEEWIYALQSLCSGSKSPRYPHLGGQVDIGQLKTHNALAVFVCILVARHCFSLADFVSKFALPTLARSVSAGGAELSVDAEAGARLTCHLVLKLFKTLEIPQPGMYSVSTSPNPLHAVGNDFSIRLSCDRHLLVGAHKTIPIAAVLAVLKAILIVVDNAALKTPLASGSGTSSGGLGGAFGSGKRSGFNTPVHPGSTPKSNEQRPADLSQILGTSDLQLGSSLTSEPEALQQPSVGGMEQISLLEFAQAVLKQICAQEHVLERCLKNAEQLCDMIIDEMLTAKQAQRVLHMICYPEPEFNIISELDQRSMIVRILENLGQWTLRISWLDLQLMYRQSLSNNAELNVWLDTVARAAIDVFHMEEVVLPGAVKATHKPKPSTWLVAPLIAKLTPAVQGRILRVAGQVLESMNYFSKVSKSDCNSSGSGDEREKSNSCHSSNSYGLGGVPARNKKMPLDYQPFLGLILTCLKGQDEYKENLLVSLYAQLSQCLQSFAELDTIGGIDEPQAREEILDALQLRFSLVGGMFEAIQKNSTPTTDWAILLAQLVCQGVVDLSCNRELFTTVVDMLATLVHSTLVSDDERHYMNLMKKLKKEIGEKNNASIRVIRQLLPLYKQPTEVIACEHSGMDTKGNKICDIDKKQLRISDKQRISVWDILEGHKNPAPLSWVWFGAVKLERKPLTYEEAHRNLKYHTHSLVKPSSYYYEPLPLPPEDIEPVPEKICIKDEMKADTPSSVDQSPSAVVGGTGRGRGKGTTTRKRKPKNPKTPPVVNTQQQQPQLAQQPQQPQNVQQQQLQQQQQQQQHMQQQHMQQQQMQPNQMGQMPMNMPMNMQQFAPNPNNMMQQNAMLQQQQQQQMQQMGNNPMQQQLNVGGGNGQPNPQMNFMQQGPGGGGAGPQGMPGQQQQWHNAPQQQQPPQPYHNQYAPHQQNMQSNRIERPPLNANSKQALSQMLRQRQPFQQQAQQGPGGGFNPMQQQPQASQQQPGPQQQMNPNQMRQQQMNPQQNPQSVAAFNAMQQQPQQNAQQQQMNPNQQQQQQFMRGGNMRPGMAPNQMNQMNMGGQGMSQNPMMQQQIPQNMVGMVNPNANQMMQSGGAQGGNGVGVGVGVGVGGAGNNPNMGMGGMPQQGMIQQQPQQQPQQQVQFQNFQNQYQQQQQQGMQQQGGGAGVGVGVGMAPNQQQQQQANMMGNFNPQMQQGNRNNPDFMAAAAVAQQQQQQQQQQRVVPGGMMAGNRNQYMNQAPNVTMSTMMGPGPGGVVGQVPPYARQQSAGGGKPGVLNTQQQFQQQQQQQQQLRHQMMQLQGMGGGAGGGMGAGPQQGGGAVGGGAGGGMVPQQQSMNQQQTPNLVAQLQRQNMMGQQQYQPPPY DIP protein Q9VW47 original sequence version: 40 68B0CD469E83A9F5 med13_drome Mediator of RNA polymerase II transcription subunit 13 skd bli pap Med13 Mediator complex subunit 13 Mediator complex subunit Skuld Protein blind spot Protein poils aux pattes Trap240 CG9936 dTRAP240 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTHQNHQTNGASLEDCHTNFYALTDLCGIKWRKFVNGERPNASSDPLADPILRSYSRCIQADMLCVWRRVQSTKTDHADPNALTFEMTTSTKVHPPLSLAAAKELWIFWYGEEPDLSELVDAELLRVAANQALWNGTWKGALTYECRSLLFKALHNLMERFVLTKDIVRFGKWFVQPCTSSDRLFGRSSQHLSFSFTFFVHGDTVCASIDLREHPAVRPLTKEHLTEAAAAFAAASSPPGSNGSAASAGGAVPNPGQDPNGASMDGLDGGEGAAKAAPPPHARKVMLAPFGIAGILTGNSYKASDPIAEKILEDWASFFPLCNKDNTDVPPVVEVVSGGHKMYHPTNYVLVTDLDDMEHMEFVEMQKMQSSVAGAAAESAVLASLSCPPGAASAPSSMGAAPSATAVPLGPASLNAPALAGAGVGATASSAAKEISRKAAPQAVSALERLAFQPYYDQRPTSGFTFNTNNTHIPASAAVEMPERTWQDCVMNTLHVDAAAAAAAVASSTPASGTGSLSADGDENEQNKPPQDSKQLVQQQIQQQQQRQKLWNFVDPMQKAPCICTKHLGNTPHGTPHGGASTYSRNSLGGDSSMPVASVESPATPAPSPHPNSAHSQPTSVPPAEQLLNMSPHAPTSVSNLQQPPTPIDHLLDKNTPAPTPTDQHDSKSITASPYVHQTPSVEPPSYTDHAAGGGPAGGQGLGTGPGSVPAQQPATPTAATSAGGAGSGGPSNAIGANAVGTISVKKLEMQQQTPSAAMAIKQEPGAQGRGVGGVTSTTEALNNFKRLYNPPKLTLKDPDSFYDEEWLKEVIYDFQYQEYWDYSTVKRPKMEKQRRPRYAKNLYEGQNHVKPVMPSPGSVYGSQLLSLDESASQAGGRGGGGQAAGSSGGGLVGIANSTASGSDVEADGSSFFQGLNIKTEPGLHSPSCKETSKSSGGNSSGGGSGSGGNLFTAEGLNPSLNDLEQLFETSSNDECSSVQIHTPPDSNNPSNGGCSAVTNTIEDLKRSTAVASAAVAAAAAAAASGAGNIQAEDLTKMFPTPPSHEQQHPNSSPCQTDVVMTDLSVDTTTTIITSSITTTCNTTITSSINTTTTACSNPSNSIMLAAAQAPVTVVAIQTVSKMVKQEYNLELGSPMEEPINDWDYVYRPPQQEKFVGSTRYAPLTNLPSQTQPPLTLPTGCFYQPTWSSHKSRAATLAKAAAAQQQQHQKHQALQQRIQLHQQKLQQLQLQNQQQQQAAAAAAAAASGGVGHQKHQHQHLHDLLSAAPRTPLTPSTVPQPLSSGGSQYLLNQLNCPQAPPGASMQQLMHRAGMSPISPGPGMGPYAARSSPMSRATPTHPPPPYPYDLAVASPATSTSSYLNRPLHSQEHPHMHGLGGGATGVVGHGTGGGGHMGMVAYTGDAGIVSGGTAMAAGSSSLLQELPEVNSVLVNILLYDTALNVFRDHNFDSSSVCVCNADTQKIGNIRGADSGVYVPLPGVSFNPFPSGAGGALAGQRMLNGPSSAGFGGMRMISAFGGSPASASMPGAGSGHGHGPNGGSNSSSCTPPSSNPHITGYVDDDPVECTCGFSAVVNRRLSHRAGLFYEDEVEITGIADDPGRNKQPTLLSIIQSLSRKNQNKQGPGETSSALDKIGAGGLPNGQLEQLGHAVFDLLLDQCSIIQTSSSSVHRALQSHRRRMSRQRRIFGNNGAPTASLASIANVLEFMDAHDVISLALEQSRLAFENQRMDNMMDFHGNGSSSSHQQQQLTAFHAPPPALRHKLAGIGAGRLTVHKWPYLPVGFTRSNKEIVRTMNAIQPMLQNAFHCKSRGGSGSKDASSYNTVSGPLTWRQFHRLAGRASGQCEPQPIPSVVVGYEKDWISVAPHSIHYWDKFLLEPYSYARDVVYVVVCPDNEHVVNCTRSYFRELSSTYEMCKLGKHTPIRGWDGFLQVGAARNNVPADRETTPLDDWLRTLEHAALAEQIRRYAVAFIHQLAPYLSRVPNDKTLLNPPDGSGNSHSKGGSSCSSNSSSVSGLPGGDLPTDNIKLEPGTEPQVQPMETNEIKQEPGVGKGGTAAGETKPTLILGDPLGMGETLEDINPSAIVLYVVNPFTFASDSCELERLALIALLRCYAELLKAVPDSVRSQMNIQIISLESVMELGPCGNRKRFSDEIRCLALNIFSQCRRHLVHAQSVKSLTGFGTAANMEAFLKTKDEPNRRAYKMYTAPFVLAPMHERNDKTDFSRSAGSMHGQNEHRYSVMYCNYCLSEDQAWLLATATDERGEMLEKICINIDVPNRARRRKAPARYVALKKLMDFIMGIISQTSQMWRLVIGRIGRIGHSELKSWSFLLSKQQLQKASKQFKDMCKQCTLMYPPTILSACLVTLEPDAKLRVMPDQFTPDERFSQISMQNPLATPQDVTCTHILVFPTSAVCAPFTRQFQNEPQVDDDFLTFEEEGNEDFSDADIGDLFWDTHMDRVSNHGSPGRMDDNRSWQSAGGNNFKCTPPQEVEEVGSLNQQPISVGYMVSTAPTGRMPAWFWSACPHLEDVCPVFLKTALHLHVPSIQSADDILNSTNAHQSGNDHPLDSNLTADVLRFVLEGYNALSWLALDSNTHDRLSCLPINVQTLMDLYYLTAAIA DIP protein Q7KTX8 original sequence version: 37 735A8A502076844E ccnc-cdk8-1 1 4 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 3 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) physical association physical association Fig 1B ccnc-med12-1 1 5 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 3 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) physical association physical association Fig 1B ccnc-med13-1 1 6 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 3 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) physical association physical association Fig 1B cdk8-med13-1 1 6 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 4 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) physical association physical association Fig 1B cdk8-med12-1 1 4 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) 5 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association Fig 1B ccnc-med12-2 2 3 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Fig 1C ccnc-med13-2 2 6 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association Fig 1C ccnc-cdk8-2 2 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association Fig 1C