IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Systematic identification of the subproteome of Escherichia coli cell envelope reveals the interaction network of membrane proteins and membrane-associated peripheral proteins. Systematic identification of the subproteome of Escherichia coli cell envelope reveals the interaction network of membrane proteins and membrane-associated peripheral proteins. J. Proteome Res. (1535-3893) 2006 imex curation Huang CZ., Lin XM., Wu LN., Zhang DF., Liu D., Wang SY., Peng XX. Ecoli were cultured in luria broth medium. Membrane vesicle preparartions were used in subsequent assays. Protein complexes were in 0.5% Triton X-100 were isolated by the native-PAGE. Individual proteins in each complex were consequently resolved into a vertical line through the SDS-PADE gel and then identified by MS. wangpeng@xmu.edu.cn 10-APR-2007: Contacted by JYOTI. orchard Only protein-protein interactions ecoli Escherichia coli (strain K12) comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel ms participant Identification by mass spectrometry ms participant Huang CZ., Lin XM., Wu LN., Zhang DF., Liu D., Wang SY., Peng XX. Ecoli were cultured in luria broth medium. Membrane vesicle preparartions were used in subsequent assays. Protein complexes were in 0.5% Triton X-100 were isolated by the native-PAGE. Individual proteins in each complex were consequently resolved into a vertical line through the SDS-PADE gel and then identified by MS. wangpeng@xmu.edu.cn Accepted 2007-APR-19 by ORCHARD. 10-APR-2007: Contacted by JYOTI. J. Proteome Res. (1535-3893) 2006 orchard Only protein-protein interactions imex curation Systematic identification of the subproteome of Escherichia coli cell envelope reveals the interaction network of membrane proteins and membrane-associated peripheral proteins. Systematic identification of the subproteome of Escherichia coli cell envelope reveals the interaction network of membrane proteins and membrane-associated peripheral proteins. J. Proteome Res. (1535-3893) 2006 imex curation Huang CZ., Lin XM., Wu LN., Zhang DF., Liu D., Wang SY., Peng XX. Ecoli were cultured in luria broth medium. Membrane vesicle preparartions were used in subsequent assays. Protein complexes were in 0.5% Triton X-100 were isolated by the native-PAGE. Individual proteins in each complex were consequently resolved into a vertical line through the SDS-PADE gel and then identified by MS. wangpeng@xmu.edu.cn 10-APR-2007: Contacted by JYOTI. orchard Only protein-protein interactions ecoli Escherichia coli (strain K12) anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Huang CZ., Lin XM., Wu LN., Zhang DF., Liu D., Wang SY., Peng XX. Ecoli were cultured in luria broth medium. Membrane vesicle preparartions were used in subsequent assays. Protein complexes were in 0.5% Triton X-100 were isolated by the native-PAGE. Individual proteins in each complex were consequently resolved into a vertical line through the SDS-PADE gel and then identified by MS. wangpeng@xmu.edu.cn Accepted 2007-APR-19 by ORCHARD. 10-APR-2007: Contacted by JYOTI. J. Proteome Res. (1535-3893) 2006 orchard Only protein-protein interactions imex curation Systematic identification of the subproteome of Escherichia coli cell envelope reveals the interaction network of membrane proteins and membrane-associated peripheral proteins. Systematic identification of the subproteome of Escherichia coli cell envelope reveals the interaction network of membrane proteins and membrane-associated peripheral proteins. J. Proteome Res. (1535-3893) 2006 imex curation Huang CZ., Lin XM., Wu LN., Zhang DF., Liu D., Wang SY., Peng XX. Ecoli were cultured in luria broth medium. Membrane vesicle preparartions were used in subsequent assays. Protein complexes were in 0.5% Triton X-100 were isolated by the native-PAGE. Individual proteins in each complex were consequently resolved into a vertical line through the SDS-PADE gel and then identified by MS. wangpeng@xmu.edu.cn 10-APR-2007: Contacted by JYOTI. orchard Only protein-protein interactions ecoli Escherichia coli (strain K12) comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel ms participant Identification by mass spectrometry ms participant Huang CZ., Lin XM., Wu LN., Zhang DF., Liu D., Wang SY., Peng XX. Ecoli were cultured in luria broth medium. Membrane vesicle preparartions were used in subsequent assays. Protein complexes were in 0.5% Triton X-100 were isolated by the native-PAGE. Individual proteins in each complex were consequently resolved into a vertical line through the SDS-PADE gel and then identified by MS. wangpeng@xmu.edu.cn Accepted 2007-APR-19 by ORCHARD. 10-APR-2007: Contacted by JYOTI. J. Proteome Res. (1535-3893) 2006 orchard Only protein-protein interactions imex curation Systematic identification of the subproteome of Escherichia coli cell envelope reveals the interaction network of membrane proteins and membrane-associated peripheral proteins. Systematic identification of the subproteome of Escherichia coli cell envelope reveals the interaction network of membrane proteins and membrane-associated peripheral proteins. J. Proteome Res. (1535-3893) 2006 imex curation Huang CZ., Lin XM., Wu LN., Zhang DF., Liu D., Wang SY., Peng XX. Ecoli were cultured in luria broth medium. Membrane vesicle preparartions were used in subsequent assays. Protein complexes were in 0.5% Triton X-100 were isolated by the native-PAGE. Individual proteins in each complex were consequently resolved into a vertical line through the SDS-PADE gel and then identified by MS. wangpeng@xmu.edu.cn 10-APR-2007: Contacted by JYOTI. orchard Only protein-protein interactions ecoli Escherichia coli (strain K12) far western blotting far western blotting Affinity blotting Affinity blotting antibody detection identification by antibody antibody detection Huang CZ., Lin XM., Wu LN., Zhang DF., Liu D., Wang SY., Peng XX. Ecoli were cultured in luria broth medium. Membrane vesicle preparartions were used in subsequent assays. Protein complexes were in 0.5% Triton X-100 were isolated by the native-PAGE. Individual proteins in each complex were consequently resolved into a vertical line through the SDS-PADE gel and then identified by MS. wangpeng@xmu.edu.cn Accepted 2007-APR-19 by ORCHARD. 10-APR-2007: Contacted by JYOTI. J. Proteome Res. (1535-3893) 2006 orchard Only protein-protein interactions imex curation pyrd_ecoli Dihydroorotate dehydrogenase (quinone) Dihydroorotate oxidase DHOdehase pyrD b0945 JW0928 protein protein ecoli Escherichia coli (strain K12) MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPAKPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFRLVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICMEKIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKIAPDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKSTEIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVTHI 973227EAE6B83622 973227EAE6B83622 ydga_ecoli Protein YdgA ydgA b1614 JW1606 protein protein ecoli Escherichia coli (strain K12) MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQLKLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFPLAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLKPLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLKTDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQLDYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELYQEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLAQEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFRLTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ DIP protein P77804 original sequence version: null D061576FA5DBAC4C atpb_ecoli ATP synthase subunit beta atpD papB uncD b3732 JW3710 ATP synthase F1 sector subunit beta F-ATPase subunit beta protein protein ecoli Escherichia coli (strain K12) MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQQQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEIGEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNMMELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRLRVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPAVDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVARARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSIEEAVEKAKKL DIP protein P0ABB4 original sequence version: 61 18DB33154B1AE6FE odp1_eco57 Pyruvate dehydrogenase E1 component aceE Z0124 ECs0118 protein protein eco57 Escherichia coli O157:H7 MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEARKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDLELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQEQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLKDTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKIINELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYVREHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTIKGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHTYLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLKNKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQILQEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFLIGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEKQENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVREAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPAVASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGELAKRGEIDKKVVADAIAKFNIDADKVNPRLA 7FB3811DE11BDD02 glpd_ecoli Aerobic glycerol-3-phosphate dehydrogenase glpD glyD b3426 JW3389 protein protein ecoli Escherichia coli (strain K12) METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSASSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIRIGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVVRKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGMHLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYKGDPKAVKIEESEINYLLNVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDYTLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIEGDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAELKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQRLSLAS 1C4D341E9E4536AB 1C4D341E9E4536AB slp_ecoli Outer membrane protein Slp slp b3506 JW3474 protein protein ecoli Escherichia coli (strain K12) MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQPGLYVGQQARFGGKVINVINGKTDTLLEISVLPLDSYAKPDIEANYQGRLLARQSGFLDPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYGYGAFWPEPGWGAPYYTNAVSQVTPELVK B2CDBC40E05ADB30 atpa_ecoli ATP synthase subunit alpha atpA papA uncA b3734 JW3712 ATP synthase F1 sector subunit alpha F-ATPase subunit alpha protein protein ecoli Escherichia coli (strain K12) MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGLADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRGLLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIGRGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGALANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLLLRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQAGDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGGIRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFAAERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFKATQSW DIP protein P0ABB0 original sequence version: 49 DIP protein P0ABB0 original sequence version: 64 DD1F3859375E9103 rl9_ecoli 50S ribosomal protein L9 rplI Large ribosomal subunit protein bL9 JW4161 b4203 protein protein ecoli Escherichia coli (strain K12) MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNIEFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADAVTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE DIP protein P0A7R1 original sequence version: 58 DIP protein P0A7R1 original sequence version: 65 DIP protein P0A7R1 original sequence version: 69 F8FF527DBB9458CA rl1_ecoli 50S ribosomal protein L1 rplA Large ribosomal subunit protein uL1 JW3947 b3984 protein protein ecoli Escherichia coli (strain K12) MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDVAVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLADQIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQVRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTMGAGVAVDQAGLSASVN DIP protein P0A7L0 original sequence version: 52 DIP protein P0A7L0 original sequence version: 59 D751704B34748D53 dada_ecoli D-amino acid dehydrogenase dadA dadR b1189 JW1178 D-alanine dehydrogenase protein protein ecoli Escherichia coli (strain K12) MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANAGQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYMENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDAGVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKFRFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYSLTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMVVRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQLLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS EE747358845B6280 ybjp_ecoli Uncharacterized lipoprotein YbjP ybjP b0865 JW0849 protein protein ecoli Escherichia coli (strain K12) MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNVAQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPDSAHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHATAGTLRQSIENR 3501F02576ACEF0F ompw_ecoli Outer membrane protein W yciD ompW b1256 JW1248 protein protein ecoli Escherichia coli (strain K12) MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGGTLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPPTLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVGVDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF FE6C1208CCF11224 dcea_ecoli Glutamate decarboxylase alpha gadA gadS b3517 JW3485 protein protein ecoli Escherichia coli (strain K12) MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQIINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT 86F963E710553E22 86F963E710553E22 frda_ecoli Fumarate reductase flavoprotein subunit frdA Quinol-fumarate reductase flavoprotein subunit JW4115 b4154 protein protein ecoli Escherichia coli (strain K12) MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRSHTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGCPWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLDILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMALSHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLGEPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICELAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRLGSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWAKIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIELGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEYSDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG DIP protein P00363 original sequence version: 131 DIP protein P00363 original sequence version: null 3306D7FF6E198AE9 frdb_ecoli Fumarate reductase iron-sulfur subunit frdB Quinol-fumarate reductase iron-sulfur subunit JW4114 b4153 protein protein ecoli Escherichia coli (strain K12) MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYIKDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERDLVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACPQFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKHVDPAAAIQQGKVESSKDFLIATLKPR AC1D7A73244D7AC0 metq_ecoli D-methionine-binding lipoprotein MetQ metQ b0197 JW0193 yaeC protein protein ecoli Escherichia coli (strain K12) MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQVAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYKLVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDGVGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGIFVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW DIP protein P28635 original sequence version: 91 DIP protein P28635 original sequence version: null B50CBC6FB5CD2BF7 ace-frda-frdb 1 16 unspecified role unspecified role neutral component neutral component 19 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component physical association physical association Figure 3 Figure 4A Table 3 Line 1 complex included seven proteins, in which OmpW, Slp, and YbjP are OMPs; YdgA is an IMP and may function in the periplasmic space as a dimer;18 and Frd flavoprotein subunit (FrdA), Frd iron-sulfur protein (FrdB), and pyruvate dehydrogenase (Odp1) are not membrane proteins annotated from the Swiss-Prot database. glpd-dcea-metq 1 20 unspecified role unspecified role neutral component neutral component 17 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component physical association physical association Figure 3 Table 3 Line 2 complex included D-methionine-binding lipoprotein (MetQ), and aerobic glycerol-3-phosphate dehydrogenase (GlpD) and glutamate decarboxylase alpha (DceA), respectively, atpa-atpb-dada-pyrid 1 7 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component physical association physical association Figure 3 Table 3 Line 3 complex consisted of four IMPs or peripheral membrane proteins, ATP synthase alpha chain (AtpA), ATP synthase beta chain (AtpB), D-amino acid dehydrogenase small subunit (DadA), and dehydroorotate dehydrogenase (PyrD), and two cytoplasimic proteins ribosomal proteins, 50S ribosomal protein L1 (RL1) and 50S ribosomal protein L9 (RL9). ompw-acee-frdb-1 2 19 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait association association Figure 4 D Further, Co-IP indicated that OmpW, FrdB and Odp1 could be complexed ompw-frdb 4 19 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey physical association physical association Figure 4 C Furthermore, a far-Western blotting technique was applied to investigate whether OmpW could be bound with the other proteins in the complex. Our result indicated that the bait protein is FrdB when OmpW was used as the prey protein ydga 3 6 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component physical association physical association Figure 3 B Out of the seven proteins in line 1 complex, Slp and YbjP moved to line 2; Odp1, FrdA, FrdB, and OmpW disappeared from the gel; and only YdgA kept at the same location. YdgA was reported to be oligomeric. glpd-dcea-metq-1 3 10 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component 17 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component 20 unspecified role unspecified role neutral component neutral component physical association physical association Figure 3 B Out of the seven proteins in line 1 complex, Slp and YbjP moved to line 2; Odp1, FrdA, FrdB, and OmpW disappeared from the gel; and only YdgA kept at the same location. atpa-atpb-dada 3 11 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component physical association physical association Figure 3 B In line 3 complex, RL1 and RL9 disappeared.