UniProt UniProt http://www.uniprot.org The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in mitochondrial trafficking. The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in mitochondrial trafficking. Biochem. Biophys. Res. Commun. (0006-291X) 2006 imex curation Fransson S., Ruusala A., Aspenstrom P. some problems with the mutations, as I see some weaker bands in fig 6. But the expression seems to vary a lot upon co-transfection (TCL bands). So I leave with the authors: Co-immunoprecipitation experiments with the Miro-1 V13, N18, V13V427, N432, and K208K328 mutants showed that the interactions with GRIF-1 and OIP106 were neither dependent on the GTP/GDP loaded status of Miro, nor on calcium-binding capacity of the EF-hands, since all mutants-bound with a similar apparent affinity to GRIF-1 and OIP106. HA-tagged GRIF-1 or OIP106 together with Myc-tagged Miro were transfected in HEK293T cells. pontus.aspenstrom@licr.uu.se davet Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Fransson S., Ruusala A., Aspenstrom P. some problems with the mutations, as I see some weaker bands in fig 6. But the expression seems to vary a lot upon co-transfection (TCL bands). So I leave with the authors: Co-immunoprecipitation experiments with the Miro-1 V13, N18, V13V427, N432, and K208K328 mutants showed that the interactions with GRIF-1 and OIP106 were neither dependent on the GTP/GDP loaded status of Miro, nor on calcium-binding capacity of the EF-hands, since all mutants-bound with a similar apparent affinity to GRIF-1 and OIP106. HA-tagged GRIF-1 or OIP106 together with Myc-tagged Miro were transfected in HEK293T cells. pontus.aspenstrom@licr.uu.se Accepted 2007-OCT-20 by DAVET. Biochem. Biophys. Res. Commun. (0006-291X) 2006 davet Only protein-protein interactions imex curation The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in mitochondrial trafficking. The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in mitochondrial trafficking. Biochem. Biophys. Res. Commun. (0006-291X) 2006 imex curation Fransson S., Ruusala A., Aspenstrom P. some problems with the mutations, as I see some weaker bands in fig 6. But the expression seems to vary a lot upon co-transfection (TCL bands). So I leave with the authors: Co-immunoprecipitation experiments with the Miro-1 V13, N18, V13V427, N432, and K208K328 mutants showed that the interactions with GRIF-1 and OIP106 were neither dependent on the GTP/GDP loaded status of Miro, nor on calcium-binding capacity of the EF-hands, since all mutants-bound with a similar apparent affinity to GRIF-1 and OIP106. HA-tagged GRIF-1 or OIP106 together with Myc-tagged Miro were transfected in HEK293T cells. pontus.aspenstrom@licr.uu.se davet Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 confocal microscopy confocal microscopy anti tag immunost anti tag immunostaining anti tag immunost Fransson S., Ruusala A., Aspenstrom P. HA-tagged GRIF-1 or OIP106 together with Myc-tagged Miro were transfected in COS-7 cells. pontus.aspenstrom@licr.uu.se Accepted 2007-OCT-20 by DAVET. Biochem. Biophys. Res. Commun. (0006-291X) 2006 davet Only protein-protein interactions imex curation trak2_rat Trafficking kinesin-binding protein 2 Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 3 protein homolog GABA-A receptor-interacting factor 1 O-GlcNAc transferase-interacting protein of 98 kDa Trak2 Als2cr3 Grif1 Oip98 protein protein rat Rattus norvegicus (Rat) Rat MSLSQNAIFKSQTGEENLMSSNHRDSESITDVCSNEDLPEVELVNLLEEQLPQYKLRVDSLFLYENQDWSQSSHQQQDASETLSPVLAEETFRYMILGTDRVEQMTKTYNDIDMVTHLLAERDRDLELAARIGQALLKRNHVLSEQNESLEEQLGQAFDQVNQLQHELSKKEELLRIVSIASEESETDSSCSTPLRFNESFSLSQGLLQLDMMHEKLKELEEENMALRSKACHIKTETFTYEEKEQKLINDCVNELRETNAQMSRMTEELSGKSDELLRYQEEISSLLSQIVDLQHKLKEHVIEKEELRLHLQASKDAQRQLTMELHELQDRNMECLGMLHESQEEIKELRNKAGPSAHLCFSQAYGVFAGESLAAEIEGTMRKKLSLDEESVFKQKAQQKRVFDTVKVANDTRGRSVTFPVLLPIPGSNRSSVIMTAKPFESGVQQTEDKTLPNQGSSTEVPGNSHPRDPPGLPEDSDLATALHRLSLRRQNYLSEKQFFAEEWERKLQILAEQEEEVSSCEALTENLASFCTDQSETTELGSAGCLRGFMPEKLQIVKPLEGSQTLHHWQQLAQPNLGTILDPRPGVITKGFTQMPKDAVYHISDLEEDEEVGITFQVQQPLQLEQKPAPPPPVTGIFLPPMTSAGGPVSVATSNPGKCLSFTNSTFTFTTCRILHPSDITQVTPSSGFPSLSCGSSAGSASNTAVNSPAASYRLSIGESITNRRDSTITFSSTRSLAKLLQERGISAKVYHSPASENPLLQLRPKALATPSTPPNSPSQSPCSSPVPFEPRVHVSENFLASRPAETFLQEMYGLRPSRAPPDVGQLKMNLVDRLKRLGIARVVKTPVPRENGKSREAEMGLQKPDSAVYLNSGGSLLGGLRRNQSLPVMMGSFGAPVCTTSPKMGILKED D0E135DBEC30C28C trak1_human Trafficking kinesin-binding protein 1 Protein Milton TRAK1 KIAA1042 OIP106 106 kDa O-GlcNAc transferase-interacting protein protein protein human Homo sapiens Human MALVFQFGQPVRAQPLPGLCHGKLIRTNACDVCNSTDLPEVEIISLLEEQLPHYKLRADTIYGYDHDDWLHTPLISPDANIDLTTEQIEETLKYFLLCAERVGQMTKTYNDIDAVTRLLEEKERDLELAARIGQSLLKKNKTLTERNELLEEQVEHIREEVSQLRHELSMKDELLQFYTSAAEESEPESVCSTPLKRNESSSSVQNYFHLDSLQKKLKDLEEENVVLRSEASQLKTETITYEEKEQQLVNDCVKELRDANVQIASISEELAKKTEDAARQQEEITHLLSQIVDLQKKAKACAVENEELVQHLGAAKDAQRQLTAELRELEDKYAECMEMLHEAQEELKNLRNKTMPNTTSRRYHSLGLFPMDSLAAEIEGTMRKELQLEEAESPDITHQKRVFETVRNINQVVKQRSLTPSPMNIPGSNQSSAMNSLLSSCVSTPRSSFYGSDIGNVVLDNKTNSIILETEAADLGNDERSKKPGTPGTPGSHDLETALRRLSLRRENYLSERRFFEEEQERKLQELAEKGELRSGSLTPTESIMSLGTHSRFSEFTGFSGMSFSSRSYLPEKLQIVKPLEGSATLHHWQQLAQPHLGGILDPRPGVVTKGFRTLDVDLDEVYCLNDFEEDDTGDHISLPRLATSTPVQHPETSAHHPGKCMSQTNSTFTFTTCRILHPSDELTRVTPSLNSAPTPACGSTSHLKSTPVATPCTPRRLSLAESFTNTRESTTTMSTSLGLVWLLKERGISAAVYDPQSWDRAGRGSLLHSYTPKMAVIPSTPPNSPMQTPTSSPPSFEFKCTSPPYDNFLASKPASSILREVREKNVRSSESQTDVSVSNLNLVDKVRRFGVAKVVNSGRAHVPTLTEEQGPLLCGPPGPAPALVPRGLVPEGLPLRCPTVTSAIGGLQLNSGIRRNRSFPTMVGSSMQMKAPVTLTSGILMGAKLSKQTSLR E834BE07CF41D9A4 miro1_human Mitochondrial Rho GTPase 1 Ras homolog gene family member T1 Rac-GTP-binding protein-like protein RHOT1 ARHT1 protein protein human Homo sapiens Human MKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHISDGVADSGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFKVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAVTVTRDKKIDLQKKQTQRNVFRCNVIGVKNCGKSGVLQALLGRNLMRQKKIREDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHVTQADLKSSTFWLRASFGATVFAVLGFAMYKALLKQR DIP protein Q8IXI2 original sequence version: 111 FE47AC3A4AF6F6C2 miro2_human Mitochondrial Rho GTPase 2 RHOT2 ARHT2 C16orf39 Ras homolog gene family member T2 protein protein human Homo sapiens Human MRRDVRILLLGEAQVGKTSLILSLVGEEFPEEVPPRAEEITIPADVTPEKVPTHIVDYSEAEQTDEELREEIHKANVVCVVYDVSEEATIEKIRTKWIPLVNGGTTQGPRVPIILVGNKSDLRSGSSMEAVLPIMSQFPEIETCVECSAKNLRNISELFYYAQKAVLHPTAPLYDPEAKQLRPACAQALTRIFRLSDQDLDQALSDEELNAFQKSCFGHPLAPQALEDVKTVVCRNVAGGVREDRLTLDGFLFLNTLFIQRGRHETTWTILRRFGYSDALELTADYLSPLIHVPPGCSTELNHLGYQFVQRVFEKHDQDRDGALSPVELQSLFSVFPAAPWGPELPRTVRTEAGRLPLHGYLCQWTLVTYLDVRSCLGHLGYLGYPTLCEQDQAHAITVTREKRLDQEKGQTQRSVLLCKVVGARGVGKSAFLQAFLGRGLGHQDTREQPPGYAIDTVQVNGQEKYLILCEVGTDGLLATSLDATCDVACLMFDGSDPKSFAHCASVYKHHYMDGQTPCLFVSSKADLPEGVAVSGPSPAEFCRKHRLPAPVPFSCAGPAEPSTTIFTQLATMAAFPHLVHAELHPSSFWLRGLLGVVGAAVAAVLSFSLYRVLVKSQ C72ECBCA33D04B6B rhot1-trak2-1 1 5 unspecified role unspecified role bait bait miro-like Miro-like sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 3 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 6A RHOT1/MIRO1 mutations V13 (constititively bound GTP), N18 (defective in nucleotide binding), 13V427, K208K328 and N432 did not change the interaction. rhot1-trak1-1 1 5 unspecified role unspecified role bait bait miro-like Miro-like sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 4 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 6B RHOT1/MIRO1 mutations V13 (constititively bound GTP), N18 (defective in nucleotide binding), 13V427, K208K328 and N432 did not change the interaction. rhot2-trak2-2 1 3 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 6 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 6A RHOT2/MIRO2 mutations V13 (constititively bound GTP) and N18 (defective in nucleotide binding) did not change the interaction. rhot2-trak1-1 1 6 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 4 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 6B RHOT2/MIRO2 mutations V13 (constititively bound GTP) and N18 (defective in nucleotide binding) did not change the interaction. rhot1-trak2-2 2 5 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 3 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 colocalization colocalization 5 rhot2-trak2-3 2 3 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 6 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 colocalization colocalization 5B rhot1-trak1-2 2 5 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 4 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 colocalization colocalization 6D rhot2-trak1-2 2 6 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 4 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 colocalization colocalization 5D