UniProt UniProt http://www.uniprot.org Cip1 and Cip2 are novel RNA-recognition-motif proteins that counteract Csx1 function during oxidative stress. Cip1 and Cip2 are novel RNA-recognition-motif proteins that counteract Csx1 function during oxidative stress. Mol. Biol. Cell (1059-1524) 2006 imex curation Martin V., Rodriguez-Gabriel MA., McDonald WH., Watt S., Yates JR., Bahler J., Russell P. prussell@scripps.edu 22-Jan-2008: Contacted by JYOTI. orchard Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast tap tandem affinity purification TAP TAP tap mudpit multidimensional protein identification technology mudpit Martin V., Rodriguez-Gabriel MA., McDonald WH., Watt S., Yates JR., Bahler J., Russell P. prussell@scripps.edu Accepted 2007-Not a month!-12 by ORCHARD. Mol. Biol. Cell (1059-1524) 2006 orchard Only protein-protein interactions imex curation csx1_schpo RNA-binding post-transcriptional regulator csx1 csx1 SPAC17A2.09c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSIDCLYRRSSLFDTSFVPLHSSIPATSKMSASNSDVNAPISPVVDEGKSELVSPTLERLVAPFNCSPSSTPLQDVAGVGSKMSDTLWMGDLEPWMDATFIQQLWASLNEPVNVKVMRSKASSSETLISYCFVQFSSSAAAERALMKYNNTMIPGAHCTFKLNWATGGGIQHNNFVSRDPEFSIFVGDLLPTTEDSDLFMTFRSIYPSCTSAKIIVDPVTGLSRKYGFVRFSSEKEQQHALMHMQGYLCQGRPLRISVASPKSRASIAADSALGIVPTSTSNRQPNQDLCSMDPLNTTVFVGGLASNLSEKDLQVCFQPFGRILNIKIPFGKGCGFVQYSEKSAAEKAINTMQGALVGTSHIRLAWGHNTLPVSALSQSQSQVSDEGFDRTLSANQIFGMNQSVIGANSGSSNSSGSSLKSAPVSPRTAAAQSLLPNSVVSSINGMNSVNFSTISPPPLSRSASISPTLSGSGSGLTPLSSHFPSAATGLVGGQVYPQSSVLQSSKINGSAKVQPSVKLPEWLQPFSGNNHNSFATQDLLTRVSSLKLVDDEQPASLNGSAFQARASRPWNLGRERQSSLIDLRHELEQNENGLEKSGFGLNLRGRLPPRSYSTFNCTGQYLQPSLRLSRDS 3852A0BDBCBD0C85 cip2_schpo RNA-binding post-transcriptional regulator cip2 Csx1-interacting protein 2 cip2 SPAC12G12.03 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MAEGTSQRPLTPLSQAFLSTNTLSPASSPLTFHSEAASLRRKRSNFFPKLDELNGAQDEEMRGLNIPGGSGYLENRYGRDKFTFSDAAVNSAGMGGSGIFSHNNADDGLGDGSSTVLPSSLKQMLDPEISTANNPSAKPRRTRLQTAWKEATNSAGLSLRSASALDVLKNSGPATTSLLSSTTYPFDSLNAFNEPSHSHVPNSASSTALSRGMSNSVTLTAPPEPDEETIPTAIVIKNIPFSLKKEVLFKVFTALDIPRPYAFNYHFDNGVFRGLAFANFHSPEEAKTVVQVLNGYEITGRRLRVEWKRQLPPAERERVERGKQEKRAVEERKNQLKSPFSVANIGAGIDFDLNDPAILNVYSHILLFYYRSDNTNDLVFDSSTTQEERRVAALLASRLNLNHSVTGDGEAKQVVITMPSTHFTPANNSSANHSPLMAPNASTLNPSSLGASNLSQPSLANHLSSSGLFDNGLFSSGGLSSNFSSLRRPAPSMHLADSIRSLRGLNDSKAFSDIRSMPATPLELATPFANLNVSSPLDRNATNSSNTLNGSAMNDYFASLTPSNTGAIGSRTFTKN 41FBD27201EE7D07 cip1_schpo RNA-binding post-transcriptional regulator cip1 Csx1-interacting protein 1 cip1 SPBC16A3.18 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MPNEIFPWKIRVDESRGLAGNSGTKKSNHEALSRLQSPLNSPKLQPIGSPQASRKTSGSGSSAPLYPKWSGALSLASSRAASPAPSDSFPTFGYSQLGGLENSSKGSALFNSANSIGTPYLSSRNSNSANEASAMAFHNVSPPSGAESSSESKSFSASGKGNKADTSAEPSLDAFNSTQIKAGSTANSNSTPVEPGEDTIPTAIVVKNIPFSLEKDTLLDHFKQLGIPRPYAFNYHYDNGIFRGLAFANFYRPEEAQVVVQTLNGYEINGRRLRVEWKRQLPAAEREKVEKAKKRQAEERRRKQQYKMFEVSFTDQGLNLNDTGTLETYSRLLLFAHQCIPSREITFETTSKDGNLLNAIRIFCLYFDLDYYARPNGEVLKLVVTHPNKKNTSVSQSQPASPNLRFNMPAPLATRFLQEHSLNGTKSAPITPPPSFAVPLTNQLRSIDDKIYGNESPLQKASTLSSPFNSKNDNDASTSASKQSFGVSHF CCE63830E9B83981 csx1-cip1-1 1 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 2 unspecified role unspecified role bait bait c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association Fig 1A We found two novel proteins that coprecipitated with Csx1-TAP, which we termed Cip1 and Cip2. Cip1 (Csx1-interacting protein 1) was identified by peptides covering 18.4% of its 490 amino acid primary sequence (Figure 1A).