UniProt UniProt http://www.uniprot.org Mon2, a relative of large Arf exchange factors, recruits Dop1 to the Golgi apparatus. Mon2, a relative of large Arf exchange factors, recruits Dop1 to the Golgi apparatus. J. Biol. Chem. 281 (2273-2280) 2006 Gillingham AK., Whyte JR., Panic B., Munro S. sean@mrc-lmb.cam.ac.uk 22-Jan-2008: Contacted by JYOTI. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Gillingham AK., Whyte JR., Panic B., Munro S. sean@mrc-lmb.cam.ac.uk Accepted 2007-DEC-30 by ORCHARD. J. Biol. Chem. 281 (2273-2280) 2006 orchard dop1_yeast Protein dopey DOP1 YD9302.17C YDR141C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLPLKPLTIDSNNKQLDSKQKKFRANVERALERFDSVTEWADYIASLGTLLKALQSWSPKFQNVRYYVPSPYQVSRRLTSSLSPALPAGVHQKTLEVYTYIFEHIGLETLATECNIWIPGILPLMTYASMSVRSHLIELYDNYILLLPQTTLRLLIRPLISSLLPGIDDESNDFLPLTLKLIETLQENLDDDSLFWQTLFLVMTANKGRRLGGLTWLTRKFPSLNAVPHLVNKIKMEAEENPSETETNDSHLDRKKRKEEAFKVLLPAAKDLVTPEPGLLIRCLVGCLEDENDILIKRSVLDLLLQRLRLDSPVLNVLITSEDKKLLIMSCCRTTLSKDMSLNRRIWNWLLGPTAGGMLNNNGGNSMEYTTSVKSANEESNVYFTKYGLSALLEGLSDLLSEEESVLTAFRISMAVMDRWEIGSLVIPELFIPLLYSSEKFKQNEQIMKTARTFFDNTETNIIWGKLFQELEDIKNLKILDFVLTNFNIGNDEEIIVRHLPLILLTLLALPSNDKDFDNIYKLQKFSLYNKLLNYIPERALLPLSHSKLKHDDEVSCEELLAKIRGFYTNVSNPSSILEKENIAERLPPFTTEDLTFLIADLIQKKLLSSLWDLENINESSKLFIAIFEKIPESEELKGRSHISWSDKKITQSIFEAIPRLCESNNDAKSEEIVGIVEIFGNYLYSRMEFIESMKLLKVVMMAVWKSLKDPRHQILGVKNLKTLNRFIPSKFIESALVYTFVEEEDISERLSVLDLLWTQLDSDSNLIRRPLELILGELFDDQNPFYLTVSKWILSILNSGSASRLFYILTDNILKVNRLEKERLDERDDLDMLTYEFQMLAYVLKTNNGRTRKVFSTELTSIKSSTIWKNEDVSTYKSLLLVTLMRFLNIKSNTHAKSIRSALILLDILLDGTEQNFKDIVIFLLQMSSKYIAEEGIEPELIAVSLLDIVSKVLRLSHDNGIKLDIFDDNAAHLKYIDFLVTSVSNMKSPLIVTAYVKLLSESIVYFENSIFRMILPLSASLVQCVQRLFLLEKREGGYYQPIALLLGGLEELLEISHGYLVTEEREGYFSGSNLKGDFIQSVVSNVFSSDSSNEESKIQGERDVILQSFRQVISCCLDIWYWAHNISCKSNDDSSLDATNHNSYKFKFRSKKLLETLFLLEPLELLENLISIRSDNTTVTLVHVLDGNKPAITIPHLLYGVIIRYNRTASVKFSNRDGSRSSTTKLTKGEPSMLKRLSGESIIAFLFNYVDSVENSAMEEFYGDFLLFFREVATNYNLYSDVSLSILKLVALISGKVSKTQFGEQKRVRREISDVFFKYLPNAFINFTNLYRGHPDSFKDLEFVVWRVQYIVNDQIGGDKFNTTLATIVNQCLTPYIKPKSEKTIPGYVLELAAVVSHLGSKVKSWRLLIAELFQNDKKLSVIGSDQTWEKIIYEWSIYPENKSKILNDLLLEIGSKRSSVTPTLITFNLGSDSEVEYKCQNLLKISYLLMVSPNDAYLLHFSSLISCIFHYLVSKDIKLKGSCWILLRVLLLRFSESHFNDYWSMISYCLQTNLQEFYESLQIQSEVDPQTILQVCKTLDLLLLLNMEGFTSTNEWIFVIDTINCVYKTNSFVALVDEIAEFKDYEITKTDDLELPTTLKDGLPLLRGIHKIERHTQLRSFFQNLSYLHYEKVYGLGSVDLYGCGEDLKKDILS 119459CD3785526C 119459CD3785526C mon2_yeast Protein MON2 YNL297C N0453 MON2 YSL2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAMNTGGFDSMQRQLEAELRSLSSESKRRNSTIRHASDKSIEILKRVHSFEELERHPDFALPFVLACQSRNAKMTTLAMQCLQGLSTVPSIPRSRLSEILDAFIEATHLAMEIQLKVLQVVPIFFKTYGKFIYGPLCKKLLLCCSNLLHVPNKAPVVVGTASATLQQLIDEIFDRLSIESVVDDKQYEVLISNSESIKVNVYRYDANKLFDNICSLNEISSNGAVSDEEMLLDIGDIPIDYGLEILESILKNSQKNLLECQDLQYLLRVKAIPLLLRCISSSRHFSTAVRSCRCLKLLIRKEYLSILELELEVILSLLIHGISVESNLSGWQRVLSLELFKDLSQDPEIVNTLYMDYDNYPDKKHVFKYLLKECIVLLNSPEYITFLAPSKVVEKMDSPLITTENSTVKTKFMHLLDKSNAPSINITYIISLILTICNHLCEGLNKSALESSPLEKKIEDKEREEGTGNDSTVVKVYSGLFSGLFELNKLFLYSTSLETSIFHLVVRAFQKLAHSAGVLSLKDKLRACMKLFSILITNNVTSSNQYSFNDTSKSAKNQHTRNISTSSVTTSPVESTKNPSRSIADSAQNKEMKRRLHPRNISSRQVSLLRALISLSISLGPIFDSESWRYTFLTWQWITYYIYGPSADFKESFYSEDIPPPPILTKSDVTSIESSLTKFFESTSSYSCSTFHLVLTRLILDSKNTLTLEQTNLNLNNDIGYHPLDAKDEIIPCIYNKAFFVNKIGELATYNCKKFLFGKNGKELWSLISTYMIKLISNREMDNDSLRLYTVRVFTDIIKKATNEVGNSDEQDNKVKQFGTLENLVIDSLMATINSIKQLDIGKQEIYNGTINVESDILFQLLLTLKEILNEFGELLMNSWTNIFNIINSPFEWTVEDTDFSVNEDIDDSSLFEGIVQKHKNMIQVSYDVFKLISDDFLQSLPMSVIKFVIDTLVNFVSQKRNLNISFSSISQFWLVGDYLRVRFNPETLNLSDEKRRSLSEKINNQKLIEIITSSSSHDWELYNGLWIYLLKNLINCTNDDRVEVKNGAVQTFFRIIDSHSVCFPPWDLIFLEVIEPLLTKEWSTEELENETDFINVTLQGLIKLYPEHFKDFKNNTTCAKEWSMLLDFLKRLLSSTSNNTKNAVILNYQTLLKEIITIEDVPSDILKKCCEIFTDYNITYSDLSTNASSKTEYDCIYELITGFPPLYQLISKYDAMTDEFVEKVLLLFNSAIKYPLLPEFVQDKTKPSSMQKAILSGLDIFMTNDSKDTEILILLQLSTISILAFDTREKITKKLGPKLPKASLNRLPTFEAISYMSCSNLRNRIAKIDQFGISTLKAKHILRILKNLAEIIKRKSLITGSESDEIPIWVLASNCFCDLSNKIFKSLQEDAENPLKDNFCDLFINVIVVTLQRINPELDNLTEIDDLNEYSKYREILLENRIIDLFNERQLDTFIYAVWDCSFLYEFDELENALMQDCGTFSELSQKLSSFDFSCIFGSTTNPRFQTKYKCSLECLQDLVNFMLNTNEKLRKLTAPYLSARIALALRRYISDEYLIGRAPIPKLRKTELATLLNGLCVILRGVLDQNSTLGNKQIGVENLQTLSPLILRTIPVSHKMDGLQDKVLELSLGFTKLD F0B3D8E6B0D994D6 mon2-dop1 1 2 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait c-terminus zz tag zz tag ZZ tag ZZ tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 2A mon2-dop1-1 1 3 unspecified role unspecified role prey prey 2 unspecified role unspecified role bait bait c-terminus zz tag zz tag ZZ tag ZZ tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 2B Immunoprecipitation of Dop1-ZZ gives a second prominent band, identified by mass spectrometry as Mon2.