IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants. AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants. Cell Death Differ. (1350-9047) 2006 imex curation Kang CH., Jung WY., Kang YH., Kim JY., Kim DG., Jeong JC., Baek DW., Jin JB., Lee JY., Kim MO., Chung WS., Mengiste T., Koiwa H., Kwak SS., Bahk JD., Lee SY., Nam JS., Yun DJ., Cho MJ. Apoptosis - Interactions involving proteins with a function related to apoptosis The CaM::HRP overlay assay was performed in the absence (5mM EGTA) or presence (1mM CaCl2)of CaCl2. The interacting proteins were detected by enhanced chemiluminescent" (ECL) detection method. djyun@gsnu.ac.kr orchard Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting immunostaining immunostaining Kang CH., Jung WY., Kang YH., Kim JY., Kim DG., Jeong JC., Baek DW., Jin JB., Lee JY., Kim MO., Chung WS., Mengiste T., Koiwa H., Kwak SS., Bahk JD., Lee SY., Nam JS., Yun DJ., Cho MJ. Apoptosis - Interactions involving proteins with a function related to apoptosis The CaM::HRP overlay assay was performed in the absence (5mM EGTA) or presence (1mM CaCl2)of CaCl2. The interacting proteins were detected by enhanced chemiluminescent" (ECL) detection method. djyun@gsnu.ac.kr Accepted 2007-DEC-23 by ORCHARD. Cell Death Differ. (1350-9047) 2006 orchard Only protein-protein interactions imex curation AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants. AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants. Cell Death Differ. (1350-9047) 2006 imex curation Kang CH., Jung WY., Kang YH., Kim JY., Kim DG., Jeong JC., Baek DW., Jin JB., Lee JY., Kim MO., Chung WS., Mengiste T., Koiwa H., Kwak SS., Bahk JD., Lee SY., Nam JS., Yun DJ., Cho MJ. Apoptosis - Interactions involving proteins with a function related to apoptosis The CaM::HRP overlay assay was performed in the absence (5mM EGTA) or presence (1mM CaCl2)of CaCl2. The interacting proteins were detected by enhanced chemiluminescent" (ECL) detection method. djyun@gsnu.ac.kr orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kang CH., Jung WY., Kang YH., Kim JY., Kim DG., Jeong JC., Baek DW., Jin JB., Lee JY., Kim MO., Chung WS., Mengiste T., Koiwa H., Kwak SS., Bahk JD., Lee SY., Nam JS., Yun DJ., Cho MJ. Apoptosis - Interactions involving proteins with a function related to apoptosis djyun@gsnu.ac.kr Accepted 2007-DEC-12 by ORCHARD. Cell Death Differ. (1350-9047) 2006 orchard Only protein-protein interactions imex curation AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants. AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants. Cell Death Differ. (1350-9047) 2006 imex curation Kang CH., Jung WY., Kang YH., Kim JY., Kim DG., Jeong JC., Baek DW., Jin JB., Lee JY., Kim MO., Chung WS., Mengiste T., Koiwa H., Kwak SS., Bahk JD., Lee SY., Nam JS., Yun DJ., Cho MJ. Apoptosis - Interactions involving proteins with a function related to apoptosis The CaM::HRP overlay assay was performed in the absence (5mM EGTA) or presence (1mM CaCl2)of CaCl2. The interacting proteins were detected by enhanced chemiluminescent" (ECL) detection method. djyun@gsnu.ac.kr orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kang CH., Jung WY., Kang YH., Kim JY., Kim DG., Jeong JC., Baek DW., Jin JB., Lee JY., Kim MO., Chung WS., Mengiste T., Koiwa H., Kwak SS., Bahk JD., Lee SY., Nam JS., Yun DJ., Cho MJ. Apoptosis - Interactions involving proteins with a function related to apoptosis The CaM::HRP overlay assay was performed in the absence (5mM EGTA) or presence (1mM CaCl2)of CaCl2. The interacting proteins were detected by enhanced chemiluminescent" (ECL) detection method. djyun@gsnu.ac.kr Accepted 2007-DEC-23 by ORCHARD. A cDNA library of 4-week-old A. thaliana (ecotype Columbia) plants that were treated with heat shock (37deree celsius) for 2 h. Cell Death Differ. (1350-9047) 2006 orchard Only protein-protein interactions imex curation hs701_arath Heat shock 70 kDa protein 1 HSP70-1 ERD2 HSC70-1 Heat shock cognate 70 kDa protein 1 Heat shock cognate protein 70-1 Heat shock protein 70-1 MED37E MED37_4 Protein EARLY-RESPONSIVE TO DEHYDRATION 2 T22P11.90 At5g02500 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNPVNTVFDAKRLIGRRFSDSSVQSDMKLWPFKIQAGPADKPMIYVEYKGEEKEFAAEEISSMVLIKMREIAEAYLGVTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHFVQEFKRKSKKDITGNPRALRRLRTSCERAKRTLSSTAQTTIEIDSLYEGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQGAILSGEGNEKVQDLLLLDVTPLSLGLETAGGVMTTLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKDEIEKMVQEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTIQDEKIGEKLPAADKKKIEDSIEQAIQWLEGNQLAEADEFEDKMKELESICNPIIAKMYQGAGGEAGGPGASGMDDDAPPASGGAGPKIEEVD BD689BA0C936A420 calm1_arath Calmodulin-1/4 CAM1 At5g37780 K22F20.2 T31G3.3 CAM4 At1g66410 T27F4.1 F28G11.13 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MADQLTDEQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVEEMIREADVDGDGQINYEEFVKIMMAK 805B06B97552ECC1 bag3_arath BAG family molecular chaperone regulator 3 BAG3 Bcl-2-associated athanogene 3 T28J14_160 At5g07220 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MMKMNTGTSPSVIGGGTSGNEWESRPGGMVVQRRTDQNSDVPRVFRVRVKYGSVYHEININSQSSFGELKKMLSDQVGLHHEDMKVLYKDKERDSKMFLDLCGVKDRSKLVVKEDPISQEKRLLAKRKNAAIEKASKSISDISFEVDRLAGQVSAFETVINKGGKVEEKSLVNLIEMLMNQLLRLDAIIADGDVKLMRKMQVQRVQKYVEALDLLKVKNSAKKVEVNKSVRHKPQTQTRFEQRDLLSFVEEEEEEPRNSNASSSSGTPAVVASKWEMFDSASTAKAAETVKPVPPRFKWEFFD EB3464BD401A9D37 bag6_arath BAG family molecular chaperone regulator 6 BAG6 Bcl-2-associated athanogene 6 CAMBP1 CaM-binding protein 1 T3F17.11 At2g46240 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MMPVYMDPSQPCQMRPQEYYYQGFGNNSQHMAMDAPPPCHGSCVHGNFPAYWPPCYPPQVPYHQCCMNRSAFHPPHASYAPSCYVHPPFPVGYQPWFDVEKDVPGKHHCGKCSSQMCDLKKDRGVVIEEHEPEIEKGEAVLPVRSTNCPYPIIWIPHENARNQEYRSSLGLGKHNQPPAEVRAPDNMTIQKSFPESWRGCFPFDESSMKSLVQNQDSKKAQNGKTVEAPFDISKFKSLLQGQDMKEAQIQKNKEELGQLTYPTSWVPSRRKRDDVEASESSNEDRKKMQNGKTVEYPFDISMIKSLIQGQDVKEAQNQKNKEEPGQVPYPIFWIPSYGKRKDVEASESKESSNEGRNLESCPSDLHRNEGQITQAKGKEGNFECNVLSDAEEKSSVINIPVANHLQEPRNIPVKLSENHLPKPTEPTKRIAKNEPVKSTKKEQSSSSSEASKLPPVCLRVDPLPKERNGGSKSVSHPKRMEKSKETKIAAPLSSKKAESRTVPEACNVKCEDANAEMKMAEGSLNALRTEKGSVESNSNLQEESNGEIIKPCEAKENREQPAKKSFTEEEAARIIQSMYRGYDVRRWEPIKKLKEIATVREQMGDVKKRIEALEASTDQHIEEKEIVVNGELVMNLLLKLDAVEGLHPSIREFRKALATELSSIQDKLDSLKNSCASAEKEAVKEQVEIKSQPSDSPVNLEHSQLTEENKMVSDTNLEKVLRLSPEEHPMSVLNRTDEKQAESAAETEEGYGLFETLATDSKQATENAAAASSTTIPEKIGEVETVVPGNPPSADGNGMTVTNVEENKAMVVESLEEPINELPQMVEETETNSIRDPENASEVSEAETNSSENENRKGEDDIVLHSEKNVELSELPVGVIDEETQPLSQDPSSSYTREGNMTAMDPKTASQEETEVDHSPNNSKGIGQQTSEPQDEKEQSPETEVIVKEQPLETEVILNEQAPEPEITEPGISKETKKLMEENQRFKETMETLVKAGREQLEVISKLTSRVKSLEKKLSHKKKTQIRRRASKPMSVSPTDAVL B5E271A02299177B calm6_arath Calmodulin-6 CAM6 F13M11 At5g21274 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLSDEEVDEMIREADVDGDGQINYEEFVKVMMAK AA6E088A90AD508A calm2_arath Calmodulin-2/3/5 CAM2 CAL1 At2g41110 T3K9.12 CAM3 At3g56800 T8M16.130 CAM5 TCH1 At2g27030 T20P8.8 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIKEADVDGDGQINYEEFVKVMMAK 722B188A90AD423A lt_sv40 Large T antigen protein protein sv40 sv40 SV40 MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDKGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLFCSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLICKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETKCDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQAVDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLPKMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLNFELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKRTQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALLLMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDDDEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFTCFKKPPTPPPEPET 3E37D4BAFC5D59BD bag5_arath BAG family molecular chaperone regulator 5, mitochondrial BAG5 Bcl-2-associated athanogene 5 F12F1.7 At1g12060 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKRSRKFSSSTTTTTVIHTFYNDHTTPPATKEIPIETPLPATKANVKTNATAAAARIQSGYRSYRIRNLYKKISSINREANRVQSIIQRQETVDAIRSDEKERLRMNETLMALLLKLDSVPGLDPTIREARRKVSRKIVGMQEILDSISETKDEIQWWNYNDLGGVDSGQGGGAWPLYWEEAVEEEMCRERGGEEMERFCAQYLGFRCFQRFLRE 1F1332D8104A0EF3 calm7_arath Calmodulin-7 CAM7 T28A8.100 At3g43810 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGQINYEEFVKVMMAK EB2B188A90AD508D p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 bag6-cam2 1 9 unspecified role unspecified role prey prey region hrp tag horseradish peroxidase tag hrp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain c-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range O82345:p.Ile575Asn mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain I N Wed May 02 16:08:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile575asn' Tue Jun 30 17:04:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile575Asn' O82345:p.Ile575Ser mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain I S Wed May 02 18:17:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile575ser' Tue Jun 30 16:56:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile575Ser' ecoli Escherichia coli (strain K12) direct interaction direct interaction Four recombinant proteins (D0, D2, D3, and CDD) containing the putative CaMBD interacted with AtCaM2::HRP, Figure 2, Figure 3 The interaction was inhibited by the presence of CaCl (calcium chloride). bag6-cam2-1 2 7 unspecified role unspecified role bait bait O82345:p.Ile575Asn mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain I N Wed May 02 17:39:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile575asn' Tue Jun 30 19:17:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile575Asn' O82345:p.Ile575Ser mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain I S Wed May 02 16:13:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile575ser' Tue Jun 30 19:54:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile575Ser' 9 unspecified role unspecified role prey prey physical association physical association Interactions between AtBAG6 and AtCaM2 in yeast. Figure 3 B p53-sv40t-3 2 13 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association 3B interactions between tumor suppressor p53 (pTD1-1) and simian virus 40 large T-antigen (pVA3-1); bag3-hsp70 1 4 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 7 AtBAG3 and AtBAG5 bound to the AtHSC70, bag5-hsp70 1 4 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 7 AtBAG3 and AtBAG5 bound to the AtHSC70, bag6-cam2-2 3 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association we employed the yeast two-hybrid screening system using full-length AtBAG6 as bait. We identified 33 yeast colonies that expressed both reporter genes (Ade�/lacZ�) in an AtBAG6-dependent manner. Sequence analyses revealed that all the AtBAG6-associated proteins are AtCaM isoforms, specifically, five AtCaM1s (accession no. At5g37780), 14 AtCaM2s (accession no. At2g41110), seven AtCaM3s (accession no. At3g56800), two AtCaM4s (accession no. At1g66410), three AtCaM6s (accession no. At5g21274) and two AtCaM7s (accession no. At3g43810). bag6-cam1 3 7 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey physical association physical association we employed the yeast two-hybrid screening system using full-length AtBAG6 as bait. We identified 33 yeast colonies that expressed both reporter genes (Ade�/lacZ�) in an AtBAG6-dependent manner. Sequence analyses revealed that all the AtBAG6-associated proteins are AtCaM isoforms, specifically, five AtCaM1s (accession no. At5g37780), 14 AtCaM2s (accession no. At2g41110), seven AtCaM3s (accession no. At3g56800), two AtCaM4s (accession no. At1g66410), three AtCaM6s (accession no. At5g21274) and two AtCaM7s (accession no. At3g43810). bag6-cam3 3 7 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey physical association physical association we employed the yeast two-hybrid screening system using full-length AtBAG6 as bait. We identified 33 yeast colonies that expressed both reporter genes (Ade�/lacZ�) in an AtBAG6-dependent manner. Sequence analyses revealed that all the AtBAG6-associated proteins are AtCaM isoforms, specifically, five AtCaM1s (accession no. At5g37780), 14 AtCaM2s (accession no. At2g41110), seven AtCaM3s (accession no. At3g56800), two AtCaM4s (accession no. At1g66410), three AtCaM6s (accession no. At5g21274) and two AtCaM7s (accession no. At3g43810). bag6-cam4 3 7 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey physical association physical association we employed the yeast two-hybrid screening system using full-length AtBAG6 as bait. We identified 33 yeast colonies that expressed both reporter genes (Ade�/lacZ�) in an AtBAG6-dependent manner. Sequence analyses revealed that all the AtBAG6-associated proteins are AtCaM isoforms, specifically, five AtCaM1s (accession no. At5g37780), 14 AtCaM2s (accession no. At2g41110), seven AtCaM3s (accession no. At3g56800), two AtCaM4s (accession no. At1g66410), three AtCaM6s (accession no. At5g21274) and two AtCaM7s (accession no. At3g43810). bag6-cam6 3 7 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey physical association physical association we employed the yeast two-hybrid screening system using full-length AtBAG6 as bait. We identified 33 yeast colonies that expressed both reporter genes (Ade�/lacZ�) in an AtBAG6-dependent manner. Sequence analyses revealed that all the AtBAG6-associated proteins are AtCaM isoforms, specifically, five AtCaM1s (accession no. At5g37780), 14 AtCaM2s (accession no. At2g41110), seven AtCaM3s (accession no. At3g56800), two AtCaM4s (accession no. At1g66410), three AtCaM6s (accession no. At5g21274) and two AtCaM7s (accession no. At3g43810). bag6-cam7 3 7 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role prey prey physical association physical association we employed the yeast two-hybrid screening system using full-length AtBAG6 as bait. We identified 33 yeast colonies that expressed both reporter genes (Ade�/lacZ�) in an AtBAG6-dependent manner. Sequence analyses revealed that all the AtBAG6-associated proteins are AtCaM isoforms, specifically, five AtCaM1s (accession no. At5g37780), 14 AtCaM2s (accession no. At2g41110), seven AtCaM3s (accession no. At3g56800), two AtCaM4s (accession no. At1g66410), three AtCaM6s (accession no. At5g21274) and two AtCaM7s (accession no. At3g43810).